FastQCFastQC Report
Fri 27 May 2016
SRR2139421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8005837
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90450.053.161311
GTATCAA167150.041.555731
CTTATAC145250.034.8922921
ATCAACG204300.033.3420453
TCAACGC206950.032.9802974
TATCAAC215250.031.9895972
CAACGCA214600.030.4140135
TATACAC180000.030.11543
AACGCAG222200.029.4925386
TTATACA181050.028.991682
ACATCTC181000.026.6471278
ACGCAGA251750.025.8730057
CGCAGAG256850.025.4212448
CATCTCC216950.022.0536779
TACACAT231450.021.9167615
GCAGAGT303400.021.351359
ACACATC226600.021.1359376
GTGGTAT46700.020.6524681
ATACACA284950.020.238834
GTACATG198650.019.8406491
Produced by FastQC (version 0.11.5)