##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139421_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8005837
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.817272447590426	33.0	31.0	34.0	30.0	34.0
2	31.489369568728417	33.0	31.0	34.0	30.0	34.0
3	31.786297922378385	33.0	31.0	34.0	30.0	34.0
4	33.15925530334929	37.0	35.0	37.0	28.0	37.0
5	34.52597361150371	37.0	35.0	37.0	30.0	37.0
6	35.172564592559155	37.0	35.0	37.0	32.0	37.0
7	35.276987153248314	37.0	35.0	37.0	32.0	37.0
8	35.4115685842717	37.0	35.0	37.0	32.0	37.0
9	37.15682407723265	39.0	37.0	39.0	33.0	39.0
10-11	37.03674181725159	39.0	37.0	39.0	33.0	39.0
12-13	36.934300623407644	39.0	37.0	39.0	32.5	39.0
14-15	38.259062044355886	40.0	38.0	41.0	33.0	41.0
16-17	38.25010451749142	40.0	38.0	41.0	32.5	41.0
18-19	38.19304571152273	40.0	38.0	41.0	32.5	41.0
20-21	38.257026809093475	40.0	38.0	41.0	32.5	41.0
22-23	38.29656797909014	40.0	38.0	41.0	32.5	41.0
24-25	38.18479493028812	40.0	38.0	41.0	32.0	41.0
26-27	38.084070718107284	40.0	38.0	41.0	32.0	41.0
28-29	38.0684332716403	40.0	38.0	41.0	32.0	41.0
30-31	38.01984567537441	40.0	37.0	41.0	32.0	41.0
32-33	37.9272705648566	40.0	37.0	41.0	32.0	41.0
34-35	37.81717419807311	40.0	37.0	41.0	31.0	41.0
36-37	37.76576411672805	40.0	37.0	41.0	31.0	41.0
38-39	37.67824606511569	40.0	37.0	41.0	31.0	41.0
40-41	37.60791410794185	40.0	37.0	41.0	31.0	41.0
42-43	37.594486810762014	40.0	37.0	41.0	31.0	41.0
44-45	37.53192008846969	40.0	36.0	41.0	31.0	41.0
46-47	37.3989309817354	40.0	36.0	41.0	30.0	41.0
48-49	37.57645599559174	40.0	36.5	41.0	31.0	41.0
50-51	37.51352332846754	40.0	36.0	41.0	30.5	41.0
52-53	37.46262367978361	40.0	36.0	41.0	30.0	41.0
54-55	37.317443550722295	39.0	36.0	41.0	30.0	41.0
56-57	37.1815467955114	39.0	35.0	41.0	30.0	41.0
58-59	37.071804010717244	39.0	35.0	41.0	30.5	41.0
60-61	36.85459932282981	39.0	35.0	41.0	30.0	41.0
62-63	36.68129790390545	38.5	35.0	40.5	30.0	41.0
64-65	36.43167202449093	38.0	35.0	40.0	30.0	41.0
66-67	36.217765458645474	37.5	34.5	40.0	30.0	41.0
68-69	35.97086903218937	37.0	34.0	40.0	30.0	41.0
70-71	35.72894189771579	36.5	34.0	39.0	30.0	41.0
72-73	35.43533402441702	36.0	34.0	39.0	30.0	41.0
74-75	35.263529366105686	36.0	34.0	39.0	30.0	40.5
76-77	35.07595571796089	35.5	34.0	38.5	30.0	40.0
78-79	34.81903294984901	35.0	34.0	37.0	30.0	39.0
80-81	34.56554632281609	35.0	34.0	37.0	30.0	39.0
82-83	34.31564597443055	35.0	34.0	36.0	30.0	39.0
84-85	34.10415465475616	35.0	34.0	36.0	30.0	37.0
86-87	33.91765980333837	35.0	34.0	36.0	29.0	37.0
88-89	33.778233059061535	35.0	34.0	35.0	29.0	37.0
90-91	33.6453087727279	35.0	34.0	35.0	29.0	36.0
92-93	33.600642611308686	35.0	34.0	35.0	29.0	36.0
94-95	33.57811801716029	35.0	34.0	35.0	29.5	36.0
96-97	33.58720722575644	35.0	34.0	35.0	30.0	36.0
98-99	33.623441470244515	35.0	34.0	35.0	30.0	35.5
100-101	33.230229706200035	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	7.0
17	72.0
18	476.0
19	1807.0
20	4690.0
21	10228.0
22	17710.0
23	27774.0
24	42190.0
25	62566.0
26	91602.0
27	127163.0
28	143328.0
29	125369.0
30	148578.0
31	183184.0
32	228754.0
33	298193.0
34	406080.0
35	573772.0
36	876098.0
37	1510186.0
38	2012718.0
39	1065665.0
40	47626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69894715568154	20.619430547986425	14.85183872716869	24.82978356916335
2	13.933801698692177	20.69745836192678	42.16184764394209	23.206892295438948
3	17.328340823139598	26.170392832271023	31.261978745397595	25.23928759919179
4	11.889037156254563	18.682299253343658	38.31791465911522	31.110748931286565
5	12.9872538834604	37.566774933857424	35.75966257509701	13.686308607585165
6	29.36711848143836	38.34083385688246	18.71637920840372	13.575668453275458
7	25.682563091405797	33.016513447864085	22.91834030441196	18.382583156318162
8	24.00077993513198	37.20017027667712	21.217870457321286	17.581179330869617
9	24.791685845429644	17.03962256444283	21.133183533922896	37.03550805620463
10-11	23.73303202272654	26.870937607588942	28.96861274093504	20.42741762874948
12-13	24.62685366002287	24.36847906326542	28.966043797359415	22.038623479352296
14-15	21.888431423626994	25.933827344187133	26.375599180572106	25.802142051613764
16-17	21.65087025373772	29.235184476904696	26.993854225660165	22.120091043697425
18-19	21.617397062807157	27.932170967915564	28.820092829135596	21.630339140141682
20-21	22.240846547414904	27.051729621938552	28.925115054520813	21.782308776125728
22-23	21.85428560146078	27.5177311877459	28.687316021275926	21.94066718951739
24-25	21.903132775195473	27.576964633802554	28.845599200624683	21.674303390377283
26-27	21.906124152641485	27.759122069413177	28.835198886773895	21.499554891171442
28-29	21.338474852024998	27.281329377362646	29.154161894886016	22.22603387572634
30-31	22.006741410829065	27.024791689954252	29.3035767723334	21.66489012688329
32-33	21.60395737175474	27.67228786009861	28.956489433305983	21.76726533484067
34-35	21.616394375918674	27.688996565244604	29.41818241359896	21.276426645237756
36-37	22.02922936886644	27.38710822182769	28.752191583069177	21.831470826236696
38-39	21.773904663280288	27.150594078508444	29.05990306194938	22.015598196261887
40-41	21.84464544740989	27.02655635728069	29.14698337051535	21.981814824794068
42-43	21.963075496358307	27.03020855847581	29.153066934561274	21.853649010604606
44-45	21.742901079283328	27.366925781750894	28.878289548572354	22.011883590393424
46-47	22.2053342416458	27.09552364900791	28.203128246456323	22.49601386288997
48-49	22.034446886584206	27.1785053391869	28.417493435328566	22.36955433890033
50-51	21.80933943498649	27.54580197403139	28.430267647709478	22.21459094327264
52-53	21.882808261749716	27.75760221307299	28.519180095419816	21.840409429757482
54-55	21.764134426012475	27.780003415718824	28.17719712807009	22.27866503019861
56-57	22.09694124408601	28.132257272715893	27.739810088381233	22.030991394816862
58-59	21.631468492582673	28.47612718711406	28.258944967827016	21.633459352476255
60-61	22.12086208152519	28.65018263557179	27.575237877195356	21.653717405707663
62-63	21.754337697052737	28.94234418684964	27.551720474535564	21.75159764156206
64-65	21.731054932982772	28.750498020843036	27.600083153986805	21.918363892187383
66-67	21.875858696027663	28.528159352768416	27.606676833293175	21.989305117910746
68-69	21.90204931370679	28.268925211328487	27.69417152293252	22.1348539520322
70-71	21.997908348181795	27.865415204797035	27.834593338859502	22.302083108161664
72-73	22.08866040559426	27.78804347543392	27.852335054240264	22.27096106473156
74-75	21.98027679236101	27.839542121708625	27.850811411363736	22.329369674566625
76-77	22.109312589484674	27.781339356797012	27.77670172624431	22.332646327474006
78-79	22.084774940765826	27.78989519341662	27.745241738230124	22.380088127587427
80-81	22.1231384968269	27.815477104625643	27.751251839209722	22.310132559337738
82-83	22.11784072247405	27.80748976952617	27.882754004271703	22.191915503728076
84-85	22.21642591196387	27.82827096899817	27.74687305714091	22.20843006189705
86-87	22.077351659881405	27.855094190471043	27.823763455267642	22.24379069437991
88-89	22.116176947200874	27.826946485419413	27.80539394066292	22.251482626716797
90-91	22.13439323604471	27.868940022852662	27.79618091146104	22.200485829641593
92-93	22.198249059358336	27.80287701138176	27.90335398513951	22.095519944120394
94-95	21.977495535357527	27.860685545358898	27.94820486447084	22.213614054812737
96-97	22.011117144576424	27.931129852024796	27.897599145209583	22.1601538581892
98-99	22.296499819168876	28.47636281372351	27.882486450434264	21.344650916673352
100-101	22.961495447182816	31.059585203001394	25.11580353452953	20.863115815286264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2132.0
1	1760.5
2	1942.5
3	2560.5
4	3258.5
5	5194.0
6	6984.0
7	8522.0
8	9619.0
9	9375.0
10	8011.5
11	6625.5
12	7140.0
13	10098.5
14	17387.0
15	27679.5
16	36102.5
17	40824.0
18	41914.0
19	39923.0
20	36865.0
21	33855.0
22	32879.5
23	36385.5
24	43855.0
25	54834.5
26	68313.5
27	83546.5
28	101348.0
29	120589.0
30	138885.5
31	157709.5
32	176828.5
33	193552.0
34	209404.0
35	225390.0
36	242115.0
37	258669.0
38	272744.0
39	284563.5
40	296187.5
41	307540.5
42	316255.0
43	324619.5
44	340960.5
45	362728.0
46	366107.5
47	361889.0
48	352811.5
49	333662.0
50	321370.5
51	309892.0
52	294450.0
53	277139.5
54	259843.0
55	240422.0
56	218916.5
57	196884.5
58	174365.0
59	149263.0
60	122510.0
61	98466.5
62	78021.5
63	60487.5
64	45688.5
65	34014.0
66	25494.0
67	19593.0
68	15038.5
69	11276.5
70	8443.5
71	6272.0
72	4651.5
73	3386.0
74	2355.5
75	1576.5
76	1065.5
77	740.0
78	522.5
79	353.0
80	238.5
81	178.5
82	142.0
83	105.0
84	77.5
85	59.5
86	52.5
87	45.5
88	31.0
89	20.5
90	13.0
91	8.5
92	7.5
93	4.5
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.637217944856984
3	0.0063953338045728385
4	8.031789805363262
5	3.2476304476346447E-4
6	0.07433326459182218
7	0.02267095870175723
8	5.371081124934219E-4
9	2.498177267411265E-4
10-11	0.26435711843746007
12-13	0.2782157568284241
14-15	9.368164752792245E-5
16-17	1.3739974970761957E-4
18-19	0.44604955109628136
20-21	1.9988801274086192E-4
22-23	1.1246776392716438E-4
24-25	0.15854276416824772
26-27	0.1449735942070095
28-29	1.063813144350057E-4
30-31	1.1278278874492932E-4
32-33	8.163821522533875E-5
34-35	3.2118071573044275E-4
36-37	1.8967182538204806E-4
38-39	0.0015256592039682396
40-41	0.0017651100293487915
42-43	0.0011585518050945128
44-45	9.210090133703917E-4
46-47	0.0014200360676073281
48-49	0.0025475278947704664
50-51	2.7935457918026196E-4
52-53	0.001487186083153799
54-55	3.9811657123115185E-4
56-57	0.009448698701950153
58-59	1.1670544376661466E-4
60-61	0.0026362654386294752
62-63	0.0013454822909302721
64-65	0.001473234620475284
66-67	0.00483032376929227
68-69	0.0020466880015746437
70-71	0.0025439156302293642
72-73	0.003618685375443437
74-75	7.427772230290651E-4
76-77	3.1068997043519705E-4
78-79	8.040773614316252E-4
80-81	3.638154143017697E-4
82-83	0.0015728709958597496
84-85	2.530826454823838E-4
86-87	4.0772160768627185E-4
88-89	8.892639966110958E-5
90-91	2.1267373501457918E-4
92-93	0.0
94-95	0.0
96-97	5.107492717353822E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	880.0
20-21	2050.0
22-23	2662.0
24-25	3559.0
26-27	4881.0
28-29	7859.0
30-31	17060.0
32-33	21174.0
34-35	27921.0
36-37	40387.0
38-39	47409.0
40-41	48569.0
42-43	56084.0
44-45	67474.0
46-47	66263.0
48-49	60496.0
50-51	54359.0
52-53	52950.0
54-55	57877.0
56-57	64621.0
58-59	75719.0
60-61	72642.0
62-63	74246.0
64-65	80630.0
66-67	86027.0
68-69	90616.0
70-71	76210.0
72-73	64839.0
74-75	65362.0
76-77	68052.0
78-79	70029.0
80-81	70325.0
82-83	67862.0
84-85	67541.0
86-87	68430.0
88-89	71665.0
90-91	74077.0
92-93	74626.0
94-95	83234.0
96-97	130428.0
98-99	649846.0
100-101	5118896.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.26257366703855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.10774872997362	77.72926312790351
2	7.003208015751339	12.082294947286895
3	1.6882453066608727	4.368971554015969
4	0.602989545145145	2.080617202341486
5	0.24858000334049288	1.0721575425155978
6	0.13642741552815746	0.7061147989320817
7	0.07014876914528001	0.4235849356232758
8	0.03858103777003937	0.2662479690231055
9	0.030058587004704502	0.23336379682383687
>10	0.07287364644437111	0.9290871147475313
>50	8.223339813009573E-4	0.04674960283928793
>100	2.9519339789147304E-4	0.04948397136434588
>500	2.1415856735750303E-5	0.01206343658309694
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.7472659011168975E-5	0.0	1.2490886337056325E-5	1.2490886337056325E-5
3	0.0	3.7472659011168975E-5	0.0	4.99635453482253E-5	1.2490886337056325E-5
4	0.0	1.1241797703350693E-4	0.0	4.99635453482253E-5	1.2490886337056325E-5
5	0.0	1.3739974970761957E-4	0.0	7.494531802233795E-5	1.2490886337056325E-5
6	4.99635453482253E-5	1.3739974970761957E-4	0.0	7.494531802233795E-5	1.2490886337056325E-5
7	4.99635453482253E-5	1.3739974970761957E-4	0.0	7.494531802233795E-5	1.2490886337056325E-5
8	4.99635453482253E-5	1.498906360446759E-4	0.0	9.99270906964506E-5	1.2490886337056325E-5
9	4.99635453482253E-5	2.4981772674112653E-4	0.0	1.6238152238173223E-4	2.498177267411265E-5
10-11	4.99635453482253E-5	2.4981772674112653E-4	0.0	1.7487240871878857E-4	2.498177267411265E-5
12-13	4.99635453482253E-5	2.5606316990965466E-4	0.0	1.7487240871878857E-4	4.371810217969714E-5
14-15	7.494531802233796E-5	3.809720332802179E-4	0.0	1.7487240871878857E-4	8.743620435939427E-5
16-17	1.7487240871878857E-4	4.3093557862844325E-4	0.0	1.7487240871878857E-4	1.3739974970761957E-4
18-19	1.7487240871878857E-4	4.4967190813402773E-4	0.0	2.3108139723554202E-4	1.3739974970761957E-4
20-21	1.7487240871878857E-4	4.684082376396122E-4	0.0	2.5606316990965466E-4	1.4364519287614775E-4
22-23	1.8111785188731672E-4	4.996354534822531E-4	0.0	2.997812720893518E-4	1.9360873822437305E-4
24-25	1.998541813929012E-4	5.808262146731191E-4	0.0	4.184446922913869E-4	2.4981772674112653E-4
26-27	1.998541813929012E-4	6.370352031898726E-4	0.0	5.808262146731191E-4	2.935358289208236E-4
28-29	1.998541813929012E-4	6.495260895269289E-4	0.0	0.0018236694052102234	2.997812720893518E-4
30-31	1.998541813929012E-4	7.68189509728964E-4	0.0	0.006570206213291628	3.0602671525788E-4
32-33	1.998541813929012E-4	7.869258392345485E-4	0.0	0.01818673050675401	3.809720332802179E-4
34-35	2.4357228357259835E-4	8.681166004254146E-4	0.0	0.03316954866805307	3.997083627858024E-4
36-37	2.6230861307818284E-4	9.61798247953337E-4	0.0	0.05676483295875247	4.0595380595433053E-4
38-39	2.6230861307818284E-4	9.992709069645061E-4	0.0	0.0927198492799691	4.184446922913869E-4
40-41	2.6230861307818284E-4	0.001074216224986844	0.0	0.13311537569400927	4.371810217969714E-4
42-43	2.6230861307818284E-4	0.001117934327166541	0.0	0.17168098725967068	4.371810217969714E-4
44-45	2.6230861307818284E-4	0.00122410686103152	0.0	0.2159986519835465	4.4967190813402773E-4
46-47	2.6230861307818284E-4	0.0013240339517279704	0.0	0.2650566080723352	4.933900103137248E-4
48-49	2.6230861307818284E-4	0.0013240339517279704	0.0	0.31447679986489857	5.620898851675346E-4
50-51	2.68554056246711E-4	0.0014864154741097027	0.0	0.3622044765587908	5.620898851675346E-4
52-53	2.7479949941523914E-4	0.0015238881331208717	0.0	0.4085331739829327	6.058079873472318E-4
54-55	2.810449425837673E-4	0.001567606235300569	0.0	0.4595334628971337	6.620169758639852E-4
56-57	2.872903857522955E-4	0.0016113243374802659	0.0	0.5137376641567897	6.807533053695698E-4
58-59	2.872903857522955E-4	0.0016363061101543786	0.0	0.5678856564279288	6.93244191706626E-4
60-61	3.0602671525788E-4	0.0016612878828284913	0.0	0.6242570264670639	6.994896348751543E-4
62-63	3.3725393110052077E-4	0.0017424786440193574	0.0	0.6458350326143287	7.244714075492669E-4
64-65	3.3725393110052077E-4	0.002029769029771653	0.0	0.6530610103603158	7.244714075492669E-4
66-67	3.3725393110052077E-4	0.0020672416887828216	0.0	0.6591690537791364	7.369622938863231E-4
68-69	3.3725393110052077E-4	0.002110959790962519	0.0	0.664521398574565	7.494531802233795E-4
70-71	3.497448174375771E-4	0.002135941563636632	0.0	0.6700048976765327	7.619440665604358E-4
72-73	3.7472659011168974E-4	0.002148432449973688	0.0	0.6739082996568628	8.243984982457174E-4
74-75	3.7472659011168974E-4	0.0022483595406701386	0.0	0.6769310941504305	8.368893845827738E-4
76-77	3.8721747644874605E-4	0.0023233048586924765	0.0	0.6797352981330996	8.368893845827738E-4
78-79	3.997083627858024E-4	0.002373268404040702	0.0	0.6823896114797241	8.368893845827738E-4
80-81	3.997083627858024E-4	0.0023982501767148144	0.0	0.6836636818861039	8.743620435939427E-4
82-83	3.997083627858024E-4	0.002416986506220399	0.0	0.6841508264532491	9.368164752792244E-4
84-85	3.997083627858024E-4	0.002423231949388927	0.0	0.6846879345657424	9.368164752792244E-4
86-87	3.997083627858024E-4	0.002454459165231568	0.0	0.6848065979859446	9.368164752792244E-4
88-89	3.997083627858024E-4	0.0026480679034559407	0.0	0.6848628069744612	9.368164752792244E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9045	0.0	53.16131	1
GTATCAA	16715	0.0	41.55573	1
CTTATAC	14525	0.0	34.892292	1
ATCAACG	20430	0.0	33.342045	3
TCAACGC	20695	0.0	32.980297	4
TATCAAC	21525	0.0	31.989597	2
CAACGCA	21460	0.0	30.414013	5
TATACAC	18000	0.0	30.1154	3
AACGCAG	22220	0.0	29.492538	6
TTATACA	18105	0.0	28.99168	2
ACATCTC	18100	0.0	26.647127	8
ACGCAGA	25175	0.0	25.873005	7
CGCAGAG	25685	0.0	25.421244	8
CATCTCC	21695	0.0	22.053677	9
TACACAT	23145	0.0	21.916761	5
GCAGAGT	30340	0.0	21.35135	9
ACACATC	22660	0.0	21.135937	6
GTGGTAT	4670	0.0	20.652468	1
ATACACA	28495	0.0	20.23883	4
GTACATG	19865	0.0	19.840649	1
>>END_MODULE