FastQCFastQC Report
Fri 27 May 2016
SRR2139420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7808881
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94350.056.531561
GTATCAA175050.041.3768651
ATCAACG210900.033.5244673
TCAACGC212750.033.2757234
TATCAAC220100.032.33242
CAACGCA221900.030.453245
AACGCAG229850.029.527336
ACGCAGA263850.025.6713167
CGCAGAG269450.025.1648718
GTGGTAT47200.024.1510871
TGGTATC44050.023.0027752
GCAGAGT311150.021.5697889
GAGTACT194300.019.95289212-13
GTACATG202450.019.6494521
TACATGG202050.019.3168752
CAGAGTA305000.019.14931510-11
AGAGTAC282900.018.7212110-11
ACATGGG208750.018.333993
GTACTTT214400.018.0135214-15
CATGGGG155250.017.5477494
Produced by FastQC (version 0.11.5)