##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139420_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7808881
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.797245853791342	33.0	31.0	34.0	30.0	34.0
2	31.46448114653047	33.0	31.0	34.0	28.0	34.0
3	31.765588052884915	33.0	31.0	34.0	30.0	34.0
4	33.13336341019923	37.0	35.0	37.0	28.0	37.0
5	34.50206002627009	37.0	35.0	37.0	30.0	37.0
6	35.131551114685955	37.0	35.0	37.0	32.0	37.0
7	35.265883165590566	37.0	35.0	37.0	32.0	37.0
8	35.41179779791752	37.0	35.0	37.0	32.0	37.0
9	37.153995303552456	39.0	37.0	39.0	33.0	39.0
10-11	37.034682946250555	39.0	37.0	39.0	33.0	39.0
12-13	36.92854949383913	39.0	37.0	39.0	32.5	39.0
14-15	38.256884565150884	40.0	38.0	41.0	32.5	41.0
16-17	38.25923413354615	40.0	38.0	41.0	32.5	41.0
18-19	38.20369499804133	40.0	38.0	41.0	33.0	41.0
20-21	38.26404617618221	40.0	38.0	41.0	32.5	41.0
22-23	38.303226510016984	40.0	38.0	41.0	32.5	41.0
24-25	38.19444298599713	40.0	38.0	41.0	32.0	41.0
26-27	38.102591140450365	40.0	38.0	41.0	32.0	41.0
28-29	38.10595526391601	40.0	38.0	41.0	32.0	41.0
30-31	38.05852193204497	40.0	37.5	41.0	32.0	41.0
32-33	37.9597333331822	40.0	37.0	41.0	32.0	41.0
34-35	37.85214849848239	40.0	37.0	41.0	31.0	41.0
36-37	37.809900817808995	40.0	37.0	41.0	31.0	41.0
38-39	37.74835928354477	40.0	37.0	41.0	31.0	41.0
40-41	37.65846993475878	40.0	37.0	41.0	31.0	41.0
42-43	37.62801573471361	40.0	37.0	41.0	31.0	41.0
44-45	37.562624917917184	40.0	36.5	41.0	31.0	41.0
46-47	37.427873448896015	40.0	36.0	41.0	30.0	41.0
48-49	37.604271144147546	40.0	37.0	41.0	31.0	41.0
50-51	37.56846192589977	40.0	36.0	41.0	31.0	41.0
52-53	37.505963412619316	40.0	36.0	41.0	31.0	41.0
54-55	37.380493541482664	39.5	36.0	41.0	30.5	41.0
56-57	37.2494134996817	39.0	35.5	41.0	30.0	41.0
58-59	37.13775413014455	39.0	35.0	41.0	30.5	41.0
60-61	36.92935698198762	39.0	35.0	41.0	30.5	41.0
62-63	36.747859843126705	38.5	35.0	41.0	30.0	41.0
64-65	36.49076351254419	38.0	35.0	40.0	30.0	41.0
66-67	36.27416665903994	37.5	35.0	40.0	30.0	41.0
68-69	36.02244029886202	37.0	34.0	40.0	30.0	41.0
70-71	35.77367131036087	36.5	34.0	39.0	30.0	41.0
72-73	35.47187013231786	36.0	34.0	39.0	30.0	41.0
74-75	35.2923091718298	36.0	34.0	39.0	30.0	40.5
76-77	35.09655657607763	35.5	34.0	38.5	30.0	40.0
78-79	34.83881448552611	35.0	34.0	37.0	30.0	39.0
80-81	34.58240010365476	35.0	34.0	37.0	30.0	39.0
82-83	34.33235098829035	35.0	34.0	36.0	30.0	39.0
84-85	34.11954098125031	35.0	34.0	36.0	30.0	37.0
86-87	33.93111002426478	35.0	34.0	36.0	29.5	37.0
88-89	33.78930850988746	35.0	34.0	35.0	29.0	37.0
90-91	33.65588518361368	35.0	34.0	35.0	29.0	36.0
92-93	33.610136271619304	35.0	34.0	35.0	29.0	36.0
94-95	33.586863638151186	35.0	34.0	35.0	29.5	36.0
96-97	33.59169255751529	35.0	34.0	35.0	30.0	36.0
98-99	33.62620746831804	35.0	34.0	35.0	30.0	35.5
100-101	33.233039397924756	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	4.0
17	62.0
18	430.0
19	1762.0
20	4808.0
21	10493.0
22	17857.0
23	27230.0
24	41099.0
25	61187.0
26	88847.0
27	122778.0
28	137512.0
29	119310.0
30	142588.0
31	176110.0
32	221668.0
33	288797.0
34	394351.0
35	560276.0
36	855680.0
37	1481723.0
38	1972176.0
39	1037105.0
40	45028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.456936147445454	20.404344745425114	14.841601504748247	24.297117602381185
2	14.252455441182674	20.7781929848406	41.86724448492366	23.10210708905306
3	17.91384747471436	25.856387185876105	30.559626678196157	25.670138661213382
4	12.210974977857466	18.173095048975508	38.74645883174642	30.8694711414206
5	13.245477505406042	37.54867231478524	35.469084195965245	13.736765983843469
6	29.74046184230711	38.209352159981485	18.64766265204145	13.402523345669959
7	26.095440526018958	32.89309274128832	23.077438392298006	17.934028340394715
8	24.000530680341416	37.446298069214855	20.925069062719142	17.628102187724583
9	25.07297795518299	17.32615431307879	21.3828770250222	36.217990706716016
10-11	23.98868608832103	27.055508068644627	28.800507594203523	20.155298248830814
12-13	24.79007937850744	24.573858136545827	29.035911000372867	21.600151484573864
14-15	21.900790965357302	25.987813482235694	26.742307519181217	25.369088033225783
16-17	21.463753929183643	29.167188508868243	27.396901268992174	21.972156292955937
18-19	21.49667019609417	28.128334562973528	29.287026061169197	21.087969179763107
20-21	22.19450771093357	27.289068030981422	29.300676617890364	21.215747640194643
22-23	21.93074013579959	27.233954944178272	29.1639148582027	21.671390061819444
24-25	21.764575555001592	27.35689388573384	29.220490818241775	21.65803974102279
26-27	21.703794967451053	27.55329021549248	29.174876338145424	21.568038478911042
28-29	21.605873430857322	27.476975557501653	29.2517506056464	21.665400405994628
30-31	21.898858608596747	27.397954156529238	29.109116319305944	21.59407091556807
32-33	21.763985691779737	27.482864195826945	29.093224555462378	21.659925556930936
34-35	21.841595166762247	27.50697871257051	29.072596168866898	21.57882995180034
36-37	21.82294911582769	27.518527914094626	28.957048933780307	21.701474036297377
38-39	21.727787485526587	27.524773758787862	29.086055529046977	21.66138322663857
40-41	21.9670010765504	27.3826655073383	28.95376730392597	21.69656611218533
42-43	21.8499307550309	27.488284853504386	28.839661060594146	21.82212333087057
44-45	21.88103136719425	27.60746685002178	28.63610284659609	21.875398936187878
46-47	22.030212864888984	27.577342830323815	28.290675712525537	22.101768592261664
48-49	21.876785820325676	27.710493136538734	28.351444508450708	22.061276534684882
50-51	21.88096603202114	27.868141975251078	28.20899731488356	22.041894677844226
52-53	22.053790686214374	27.831341073003703	28.091151650065832	22.0237165907161
54-55	21.95112458287569	27.78625513805783	28.117539275036634	22.145081004029844
56-57	21.99670445783993	27.885280257013072	28.068127988405877	22.049887296741122
58-59	21.950105221277898	27.95424512307978	28.124137914604177	21.97151174103815
60-61	22.061026249601024	27.897172142792652	28.063502555076465	21.978299052529863
62-63	21.98112490624523	28.017361627717413	28.009634048185482	21.991879417851877
64-65	21.973815312420793	27.96018466933664	27.94253822014768	22.123461798094887
66-67	22.028740528258044	27.98285412162266	27.892437592521684	22.095967757597613
68-69	21.987406236929345	27.90181095691328	27.96792221488953	22.142860591267844
70-71	22.0038127950287	27.733367880063952	27.965804940916154	22.297014383991197
72-73	22.05867048360283	27.76385689471369	27.909571414726514	22.267901206956964
74-75	21.95671760097038	27.870009587320965	27.909905358865622	22.263367452843035
76-77	22.097742341374698	27.78683224034481	27.897285042933788	22.2181403753467
78-79	22.070545323000413	27.818334595007265	27.811035211322142	22.30008487067018
80-81	22.052954515986567	27.868436743316323	27.79788328790101	22.280725452796105
82-83	22.10268377350789	27.846839113897197	27.904715049832703	22.145762062762213
84-85	22.18826462874738	27.82273725216327	27.84233040071032	22.14666771837904
86-87	22.033318464840193	27.865818223130706	27.934910011020037	22.165953301009065
88-89	22.08391711644693	27.824663759080494	27.89490734035742	22.196511784115156
90-91	22.077698733846756	27.890076360788406	27.903977940216684	22.12824696514815
92-93	22.107141436526643	27.860442577242555	27.936836361493704	22.095579624737095
94-95	21.970867501060955	27.89672685565642	27.969233614169998	22.163172029112623
96-97	21.977574729791243	27.941987666601236	27.996750810497073	22.083686793110452
98-99	22.270874596411087	28.494364751989803	28.002417351899762	21.232343299699355
100-101	22.910464188185948	31.104372519860096	25.207612123391932	20.777551168562027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2345.0
1	1856.5
2	2006.0
3	2772.5
4	3418.0
5	5162.5
6	6906.0
7	8756.0
8	10430.5
9	10528.5
10	9040.5
11	7466.0
12	7846.0
13	10585.0
14	17687.0
15	27485.0
16	35526.5
17	40095.5
18	41028.5
19	39268.5
20	36208.5
21	33291.0
22	32694.0
23	35957.0
24	42989.0
25	53896.0
26	68125.5
27	83711.5
28	100632.5
29	118597.5
30	136211.0
31	153913.0
32	171670.5
33	189869.5
34	207722.5
35	223557.5
36	240272.0
37	256380.5
38	269601.0
39	280168.5
40	289744.5
41	299475.5
42	305028.0
43	309561.0
44	316922.5
45	324121.0
46	325386.0
47	323570.0
48	321237.5
49	314681.0
50	305633.0
51	295131.0
52	281959.0
53	266934.0
54	251576.0
55	234261.0
56	214743.0
57	193711.0
58	172174.5
59	149203.0
60	124961.5
61	102043.5
62	81400.0
63	63325.5
64	47714.5
65	35415.5
66	26546.0
67	20289.0
68	15267.5
69	11206.0
70	8295.5
71	6067.5
72	4338.5
73	3057.0
74	2126.0
75	1480.5
76	1036.5
77	714.0
78	485.0
79	351.5
80	246.5
81	164.5
82	124.5
83	90.0
84	64.5
85	48.5
86	39.5
87	32.0
88	21.0
89	12.5
90	8.0
91	5.5
92	4.0
93	2.0
94	1.5
95	2.0
96	1.5
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.633691178031782
3	0.006364548262420697
4	8.087176639008842
5	3.457601671737602E-4
6	0.07463297238106202
7	0.02384464560287191
8	4.73819488349227E-4
9	2.0489491388074683E-4
10-11	0.26597921008144443
12-13	0.28108380701409075
14-15	7.04326266465067E-5
16-17	8.964152482282672E-5
18-19	0.43859036909385607
20-21	1.9212055949349333E-4
22-23	1.4092655881429005E-4
24-25	0.15796528967319942
26-27	0.1348617839811178
28-29	1.2188933603029528E-4
30-31	8.350164803348801E-5
32-33	7.081105306464445E-5
34-35	3.808551230339565E-4
36-37	2.5918763467632487E-4
38-39	0.0014982098021353398
40-41	0.0017035808614484238
42-43	0.0011141304706113477
44-45	8.767386491237462E-4
46-47	0.0013466767334660758
48-49	0.0025265584187257164
50-51	2.8588883348847343E-4
52-53	0.0014735267217217372
54-55	4.5545668158402313E-4
56-57	0.007890934630107052
58-59	1.1911960799839117E-4
60-61	0.0028328493823434647
62-63	0.001730662974965497
64-65	0.0015369483459670725
66-67	0.004637644864808305
68-69	0.0018798709779447248
70-71	0.002496401820837082
72-73	0.0036602481618435005
74-75	6.995250375430574E-4
76-77	3.4162891856910323E-4
78-79	7.897380063332387E-4
80-81	4.802166241700773E-4
82-83	0.001682411692557809
84-85	2.845305932826437E-4
86-87	4.3910696182531816E-4
88-89	8.875243605265956E-5
90-91	2.9369447746694385E-4
92-93	0.0
94-95	0.0
96-97	5.083210030190031E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	793.0
20-21	1997.0
22-23	2616.0
24-25	3367.0
26-27	4540.0
28-29	7479.0
30-31	16332.0
32-33	20040.0
34-35	26487.0
36-37	38067.0
38-39	45094.0
40-41	45385.0
42-43	53233.0
44-45	64264.0
46-47	62983.0
48-49	57711.0
50-51	51055.0
52-53	49062.0
54-55	52772.0
56-57	55587.0
58-59	57919.0
60-61	57355.0
62-63	58013.0
64-65	60653.0
66-67	64253.0
68-69	65648.0
70-71	64132.0
72-73	59634.0
74-75	60724.0
76-77	63815.0
78-79	65920.0
80-81	66304.0
82-83	63862.0
84-85	63017.0
86-87	65124.0
88-89	67470.0
90-91	69881.0
92-93	71287.0
94-95	78911.0
96-97	126372.0
98-99	648324.0
100-101	5151399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.9068524210385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.63547579595841	78.76843919109963
2	6.755631014685323	11.74221255208496
3	1.5142003231277348	3.947831520538525
4	0.5388600435801211	1.873225211320478
5	0.22771494002685821	0.98949943434899
6	0.11478667056609206	0.5985448943273834
7	0.061776343441674056	0.3758151293817869
8	0.04111704856560748	0.2858682617343937
9	0.02756812445744959	0.21562730313735542
>10	0.08118541020080615	1.0560238541963631
>50	0.0014324297159784946	0.08418504594362959
>100	1.8597864053447987E-4	0.029502570726089923
>500	6.58770334275919E-5	0.03322503116043313
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2805932117546675E-5	0.0
2	0.0	2.561186423509335E-5	0.0	1.2805932117546675E-5	1.2805932117546675E-5
3	0.0	2.561186423509335E-5	0.0	1.2805932117546675E-5	1.2805932117546675E-5
4	0.0	6.402966058773338E-5	0.0	2.561186423509335E-5	1.2805932117546675E-5
5	0.0	7.683559270528006E-5	0.0	3.841779635264003E-5	1.2805932117546675E-5
6	0.0	7.683559270528006E-5	0.0	3.841779635264003E-5	3.841779635264003E-5
7	0.0	7.683559270528006E-5	0.0	3.841779635264003E-5	3.841779635264003E-5
8	0.0	7.683559270528006E-5	0.0	3.841779635264003E-5	3.841779635264003E-5
9	0.0	1.024474569403734E-4	0.0	7.683559270528006E-5	5.12237284701867E-5
10-11	0.0	1.024474569403734E-4	0.0	1.2805932117546676E-4	5.12237284701867E-5
12-13	0.0	1.024474569403734E-4	0.0	1.4726821935178678E-4	5.12237284701867E-5
14-15	0.0	1.344622872342401E-4	0.0	1.7288008358688013E-4	5.12237284701867E-5
16-17	0.0	1.4086525329301344E-4	0.0	1.7928304964565344E-4	5.12237284701867E-5
18-19	0.0	1.4086525329301344E-4	0.0	1.7928304964565344E-4	6.402966058773338E-5
20-21	0.0	1.4086525329301344E-4	0.0	1.9849194782197346E-4	6.402966058773338E-5
22-23	0.0	1.5367118541056011E-4	0.0	2.8173050658602687E-4	7.043262664650672E-5
24-25	0.0	1.8568601570442678E-4	0.0	3.0734237082112023E-4	8.323855876405338E-5
26-27	0.0	1.9849194782197346E-4	0.0	6.018788095246937E-4	8.964152482282672E-5
28-29	0.0	2.048949138807468E-4	0.0	0.0017992334625153079	8.964152482282672E-5
30-31	0.0	2.689245744684802E-4	0.0	0.006153250382481178	8.964152482282672E-5
32-33	0.0	2.689245744684802E-4	0.0	0.017255993528394145	1.2805932117546676E-4
34-35	0.0	3.521631332325336E-4	0.0	0.03226454597015885	1.2805932117546676E-4
36-37	0.0	4.225957598790403E-4	0.0	0.05571861064344558	1.4086525329301344E-4
38-39	0.0	4.2899872593781365E-4	0.0	0.09108219218605074	1.5367118541056011E-4
40-41	0.0	4.994313525843203E-4	0.0	0.13031956819421375	1.5367118541056011E-4
42-43	0.0	5.122372847018669E-4	0.0	0.16773850184168515	1.7288008358688013E-4
44-45	0.0	6.338936398185604E-4	0.0	0.2104654943518796	1.9208898176320012E-4
46-47	0.0	7.555499949352539E-4	0.0	0.258250830048505	1.9208898176320012E-4
48-49	0.0	7.683559270528005E-4	0.0	0.3050437060060206	2.048949138807468E-4
50-51	0.0	8.579974518756273E-4	0.0	0.35102340527407194	2.048949138807468E-4
52-53	0.0	8.644004179344006E-4	0.0	0.3948901257427281	2.1129787993952014E-4
54-55	0.0	8.772063500519472E-4	0.0	0.44162537500571464	2.1770084599829348E-4
56-57	0.0	8.836093161107207E-4	0.0	0.49114591450426764	2.1770084599829348E-4
58-59	0.0	8.836093161107207E-4	0.0	0.5426449705149816	2.3050677811584016E-4
60-61	0.0	9.156241464045873E-4	0.0	0.5935741625464648	2.3050677811584016E-4
62-63	0.0	9.348330445809073E-4	0.0	0.613877967918835	2.4331271023338684E-4
64-65	0.0	0.0010949071960502407	0.0	0.6207163356696049	2.561186423509335E-4
66-67	0.0	0.0011141160942265606	0.0	0.6256338136027428	2.561186423509335E-4
68-69	0.0	0.0011269220263441075	0.0	0.6304616500110579	2.625216084097069E-4
70-71	0.0	0.0011269220263441075	0.0	0.6347324283722597	2.8173050658602687E-4
72-73	0.0	0.0011269220263441075	0.0	0.6382092389421736	3.0734237082112023E-4
74-75	0.0	0.0011397279584616541	0.0	0.6411546033292094	3.0734237082112023E-4
76-77	0.0	0.001158936856637974	0.0	0.6437798194133064	3.0734237082112023E-4
78-79	0.0	0.0011653398226967474	0.0	0.6460592753302298	3.0734237082112023E-4
80-81	0.0	0.0011653398226967474	0.0	0.6471797943905151	3.137453368798936E-4
82-83	0.0	0.0012037576190493874	0.0	0.647576778286159	3.201483029386669E-4
84-85	0.0	0.0012293694832844807	0.0	0.6479417473515092	3.201483029386669E-4
86-87	0.0	0.0012421754154020275	0.0	0.6479993740460381	3.201483029386669E-4
88-89	0.0	0.0013254139741660808	0.0	0.6480313888763319	3.201483029386669E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9435	0.0	56.53156	1
GTATCAA	17505	0.0	41.376865	1
ATCAACG	21090	0.0	33.524467	3
TCAACGC	21275	0.0	33.275723	4
TATCAAC	22010	0.0	32.3324	2
CAACGCA	22190	0.0	30.45324	5
AACGCAG	22985	0.0	29.52733	6
ACGCAGA	26385	0.0	25.671316	7
CGCAGAG	26945	0.0	25.164871	8
GTGGTAT	4720	0.0	24.151087	1
TGGTATC	4405	0.0	23.002775	2
GCAGAGT	31115	0.0	21.569788	9
GAGTACT	19430	0.0	19.952892	12-13
GTACATG	20245	0.0	19.649452	1
TACATGG	20205	0.0	19.316875	2
CAGAGTA	30500	0.0	19.149315	10-11
AGAGTAC	28290	0.0	18.72121	10-11
ACATGGG	20875	0.0	18.33399	3
GTACTTT	21440	0.0	18.01352	14-15
CATGGGG	15525	0.0	17.547749	4
>>END_MODULE