FastQCFastQC Report
Fri 27 May 2016
SRR2139419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4781423
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87950.054.074651
GTATCAA161650.037.9019431
ATCAACG192150.031.4217513
TCAACGC199150.030.4034524
AACGCAG200900.030.0944396
TATCAAC203050.029.901812
ACGCAGA203050.029.8988557
CAACGCA202700.029.8120165
CGCAGAG224950.027.726438
TGGTATC34250.026.7275312
GTGGTAT36250.026.4271831
GAGTACT169400.023.9764512-13
GCAGAGT246600.023.8302549
GTACATG146700.023.102521
ACATGGG143500.022.6988623
TACATGG145950.022.5796812
CAGAGTA235600.022.41119410-11
GTACTTT186200.021.2827114-15
AGAGTAC221750.021.24686210-11
CATGGGG104350.020.5248134
Produced by FastQC (version 0.11.5)