##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139419_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4781423
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88640892052429	33.0	33.0	33.0	33.0	33.0
2	32.94677714144931	33.0	33.0	33.0	33.0	33.0
3	33.592433465936814	33.0	33.0	33.0	33.0	37.0
4	36.766480397153735	37.0	37.0	37.0	37.0	37.0
5	36.77983520805417	37.0	37.0	37.0	37.0	37.0
6	36.74858216894845	37.0	37.0	37.0	37.0	37.0
7	36.73860354961274	37.0	37.0	37.0	37.0	37.0
8	36.72997180964746	37.0	37.0	37.0	37.0	37.0
9	36.74771966420875	37.0	37.0	37.0	37.0	37.0
10-11	36.70549530965991	37.0	37.0	37.0	37.0	37.0
12-13	36.81027321782658	37.0	37.0	37.0	37.0	38.5
14-15	35.129620198840385	40.0	37.0	40.0	19.5	40.0
16-17	36.176181023933665	40.0	37.0	40.0	24.0	40.0
18-19	38.397380131396034	40.0	37.0	40.0	37.0	40.0
20-21	38.74175394615223	40.0	37.0	40.0	37.0	40.0
22-23	38.79110664549964	40.0	37.0	40.0	37.0	40.0
24-25	38.809690388168846	40.0	37.0	40.0	37.0	40.0
26-27	38.76161387091943	40.0	37.0	40.0	37.0	40.0
28-29	38.69421435601576	40.0	37.0	40.0	37.0	40.0
30-31	38.56142347646295	40.0	37.0	40.0	37.0	40.0
32-33	38.3929359248047	40.0	37.0	40.0	37.0	40.0
34-35	38.314116508518765	40.0	37.0	40.0	37.0	40.0
36-37	38.2428709001928	40.0	37.0	40.0	37.0	40.0
38-39	38.24366048580991	40.0	37.0	40.0	37.0	40.0
40-41	38.192595692260156	40.0	37.0	40.0	37.0	40.0
42-43	38.15036336976526	40.0	37.0	40.0	37.0	40.0
44-45	38.09166809771828	40.0	37.0	40.0	37.0	40.0
46-47	37.93764573357805	40.0	37.0	40.0	33.0	40.0
48-49	37.74780259595882	40.0	37.0	40.0	33.0	40.0
50-51	37.669405859255946	40.0	37.0	40.0	33.0	40.0
52-53	37.598824083928534	40.0	37.0	40.0	33.0	40.0
54-55	37.40716894469021	40.0	37.0	40.0	33.0	40.0
56-57	37.321370843268184	38.5	37.0	40.0	33.0	40.0
58-59	37.259411812630084	37.0	37.0	40.0	33.0	40.0
60-61	37.140256414113416	37.0	37.0	40.0	33.0	40.0
62-63	36.9579794807277	37.0	37.0	40.0	33.0	40.0
64-65	36.68697628405525	37.0	37.0	40.0	33.0	40.0
66-67	36.37335051599664	37.0	37.0	40.0	33.0	40.0
68-69	36.08722652518215	37.0	37.0	40.0	33.0	40.0
70-71	35.84869310291056	37.0	35.0	37.0	33.0	40.0
72-73	35.612648813462556	37.0	33.0	37.0	33.0	40.0
74-75	34.96911455187482	37.0	33.0	37.0	30.0	40.0
76-77	32.834539339873544	33.0	30.0	37.0	27.0	37.0
78-79	34.666786864858835	37.0	33.0	37.0	30.0	37.0
80-81	34.92177444058858	37.0	33.0	37.0	33.0	37.0
82-83	34.87546673785377	37.0	33.0	37.0	33.0	37.0
84-85	34.81051224292072	37.0	33.0	37.0	33.0	37.0
86-87	34.62650410297802	37.0	33.0	37.0	33.0	37.0
88-89	34.413073884946954	37.0	33.0	37.0	33.0	37.0
90-91	34.26708550151308	37.0	33.0	37.0	27.0	37.0
92-93	34.261411128748875	37.0	33.0	37.0	33.0	37.0
94-95	34.16887103072191	37.0	33.0	37.0	27.0	37.0
96-97	34.08774480286693	37.0	33.0	37.0	27.0	37.0
98-99	34.04534767347529	35.0	33.0	37.0	27.0	37.0
100	34.0129689338531	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	6.0
20	23.0
21	69.0
22	302.0
23	969.0
24	2378.0
25	5137.0
26	9658.0
27	16354.0
28	25992.0
29	38851.0
30	55626.0
31	79648.0
32	113822.0
33	167192.0
34	256168.0
35	436489.0
36	836705.0
37	1610309.0
38	1100332.0
39	25388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.132002626834705	0.0	24.04392418988501	28.82407318328028
2	15.122082827465263	24.06882106335298	39.00120990047863	21.807886208703124
3	16.994239939030066	25.010765736118056	32.863531040142654	25.131463284709227
4	12.944587731281826	23.353974445956787	37.6243674441523	26.07707037860909
5	17.312981646128353	36.72386831525316	32.172115676009625	13.791034362608857
6	27.775083317386173	37.7055746050071	20.37438707997528	14.144954997631453
7	25.974064815165328	33.599727777393184	22.340261108662958	18.08594629877853
8	24.02125659798226	32.91873946173766	22.083994055280208	20.976009884999872
9	24.243184139702862	20.534815704692484	23.58546167315159	31.636538482453062
10-11	24.6997795558006	26.40299048625207	28.576780079851023	20.320449878096312
12-13	25.278920636930362	24.657668061435803	28.638021668008612	21.425389633625223
14-15	22.81243488407433	26.673288207726557	26.977259361152907	23.537017547046204
16-17	21.304285356054045	29.070906297142084	27.94460979503382	21.68019855177005
18-19	21.252731665865998	27.824227222732645	30.259286827373355	20.663754284027995
20-21	22.115624940522043	27.220533077273974	30.299865117064122	20.36397686513986
22-23	21.894792088739262	26.99062248390694	30.096567769283272	21.018017658070523
24-25	21.499406126994753	27.228127700641547	30.37772122907384	20.894744943289865
26-27	21.42640914596329	27.47845492185259	30.156397429847022	20.9387385023371
28-29	21.42896808973224	27.51433106837316	30.15926319815171	20.897437643742894
30-31	21.76614950238124	27.335574721290058	30.086625594837546	20.811650181491153
32-33	21.54841424012181	27.680483066942717	29.908521899509914	20.862580793425554
34-35	21.687962347368078	27.558995550507483	29.82359021259155	20.929451889532892
36-37	21.621188765835672	27.61452282670555	29.841365692440302	20.922922715018476
38-39	21.49649043983944	27.949990887011662	29.71625757119787	20.837261101951025
40-41	21.866741259455274	27.65996324248896	29.458549950224207	21.014745547831563
42-43	21.73253200393434	27.768777751836144	29.319658945260596	21.179031298968916
44-45	21.842317281982133	28.19129266488567	28.813960588616293	21.1524294645159
46-47	21.953524231316237	28.063579641368598	28.468616666922863	21.514279460392302
48-49	21.860681848528365	28.33971981589926	28.352145798192236	21.447452537380133
50-51	21.927589027816463	28.56849280517948	28.038723289461853	21.465194877542203
52-53	22.210687377695397	28.34084791821092	27.835553247850303	21.612911456243378
54-55	22.083207572025792	28.342568799078176	27.984756339898492	21.58946728899754
56-57	22.07452435103638	28.620843158487453	27.779794676141627	21.524837814334543
58-59	22.14843205771449	28.390441460573708	27.848696883293428	21.612429598418377
60-61	22.047476185830014	28.480901243774813	27.971821689902583	21.49980088049259
62-63	22.102523752690846	28.718999432399666	27.753815986395878	21.424660828513606
64-65	22.131313425999203	28.458757838768374	27.71509851481771	21.694830220414715
66-67	22.122993810753254	28.428065501739407	27.73425330923438	21.714687378272963
68-69	22.133271152430957	28.607058522512062	27.731610510708606	21.528059814348378
70-71	22.188475507307892	28.247328983832226	27.732008338438057	21.832187170421825
72-73	22.222615634769895	28.322790337572044	27.703556356011934	21.751037671646127
74-75	21.978725676325542	28.537592115991554	27.745251847217233	21.738430360465667
76-77	22.259575208787908	28.37966283362188	27.50300521208383	21.85775674550639
78-79	22.057355184174575	28.41490130815473	27.6763040378688	21.851439469801896
80-81	22.134138332247737	28.507060031639558	27.528659628486	21.830142007626705
82-83	22.253154736097482	28.328508258022143	27.687118834759193	21.73121817112118
84-85	22.348188216989	28.33675788626005	27.652444837453555	21.662609059297395
86-87	22.08261738250287	28.453927027718585	27.653233856128683	21.81022173364986
88-89	22.205630810691694	28.322511129568085	27.645376845497644	21.826481214242584
90-91	22.25408456820995	28.310829681153884	27.712096960268866	21.722988790367296
92-93	22.179044758770285	28.381195934642662	27.68228286691189	21.757476439675166
94-95	22.287018248622704	28.343589719674622	27.647460299651254	21.721931732051416
96-97	22.27855702172835	28.6141117049485	27.755651794759522	21.35167947856363
98-99	23.25670725521475	30.743744391710425	27.28013384829877	18.719414504776054
100	22.689315663777773	30.40901466672604	21.723034410380283	25.178635259115907
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1015.0
1	964.5
2	1635.5
3	2571.5
4	3812.5
5	6588.5
6	8711.0
7	9902.5
8	10808.0
9	10705.5
10	9929.5
11	9120.0
12	9878.5
13	12947.5
14	20804.0
15	32554.0
16	43322.0
17	50129.0
18	50302.5
19	45250.5
20	38613.5
21	32015.5
22	27209.5
23	25933.0
24	27893.0
25	32658.0
26	40214.0
27	49276.0
28	58696.5
29	69063.0
30	79848.0
31	90119.0
32	101483.0
33	112577.5
34	123281.5
35	133602.5
36	144502.5
37	154704.5
38	160727.5
39	165699.0
40	172576.5
41	178131.0
42	180255.5
43	182604.5
44	184904.5
45	186777.0
46	187515.5
47	186868.0
48	185364.0
49	181581.0
50	176861.5
51	170321.0
52	161039.5
53	150433.5
54	139921.5
55	129153.5
56	117626.5
57	105622.5
58	93425.0
59	81269.0
60	68099.0
61	55451.5
62	44072.0
63	33947.5
64	25551.0
65	19350.5
66	14877.0
67	11434.5
68	8925.5
69	6538.5
70	4656.0
71	3485.5
72	2577.5
73	1856.5
74	1342.5
75	977.0
76	725.0
77	531.5
78	368.5
79	247.0
80	176.5
81	141.0
82	106.5
83	74.0
84	50.5
85	30.5
86	17.0
87	13.0
88	11.0
89	8.0
90	5.5
91	4.0
92	3.0
93	2.5
94	2.5
95	2.0
96	1.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	6.274282781506677E-5
2	7.947424856575124E-4
3	0.0010457137969177796
4	5.646854503356009E-4
5	7.110853819040901E-4
6	1.6731420750684472E-4
7	8.783995894109349E-4
8	7.738282097191568E-4
9	0.0015685706953766693
10-11	0.008250681857681281
12-13	0.2296387498031444
14-15	7.307939498345995
16-17	0.0
18-19	0.0
20-21	0.0
22-23	3.346935138279671E-4
24-25	0.010232549866678663
26-27	0.02227754152672975
28-29	0.007103472235900655
30-31	3.147426396646858E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	9.671917801594577E-5
46-47	0.001861905903172666
48-49	0.03268926402846145
50-51	0.07186362611277394
52-53	0.05186332061037101
54-55	0.034506441202357775
56-57	0.00506778299596591
58-59	0.01536259964963798
60-61	0.0444551796649664
62-63	0.036047795148892246
64-65	3.445726816345977E-4
66-67	0.0067663466753387315
68-69	0.04071362343533676
70-71	0.0843126242089891
72-73	0.07180474505351335
74-75	0.0342581440110161
76-77	0.00733042258983469
78-79	8.488297305765931E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	2.5473005046584395E-5
88-89	1.425080167237044E-4
90-91	0.0019919982354325563
92-93	0.0033870558017443336
94-95	0.0010424070385517785
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	144.0
20-21	511.0
22-23	1227.0
24-25	2606.0
26-27	3495.0
28-29	5365.0
30-31	9496.0
32-33	10112.0
34-35	12344.0
36-37	13748.0
38-39	16534.0
40-41	20740.0
42-43	24852.0
44-45	32794.0
46-47	32236.0
48-49	25730.0
50-51	30516.0
52-53	32821.0
54-55	28303.0
56-57	35265.0
58-59	37464.0
60-61	24446.0
62-63	19649.0
64-65	39589.0
66-67	38600.0
68-69	19718.0
70-71	28471.0
72-73	38871.0
74-75	35108.0
76-77	27407.0
78-79	41869.0
80-81	51130.0
82-83	37635.0
84-85	60479.0
86-87	66549.0
88-89	67900.0
90-91	70047.0
92-93	72585.0
94-95	86296.0
96-97	244601.0
98-99	841521.0
100-101	2492649.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.32974561399344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.9665174205746	83.97641869219393
2	5.376988258531812	9.714039631252163
3	1.0261625227273574	2.7807899890972787
4	0.3187919158691572	1.151855706570343
5	0.130728813816745	0.5904350248242843
6	0.06310606096968352	0.3420212660455604
7	0.035714749453762025	0.22582729629780843
8	0.020923789306148854	0.15120324522441778
9	0.0138023108818911	0.11220833077618289
>10	0.04500304361237447	0.6272297568549589
>50	0.001216233416205151	0.0743096959141542
>100	8.883236269598563E-4	0.17491997223630834
>500	1.5655721322407385E-4	0.07874139271259467
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.091427593835559E-5	0.0	0.0	0.0
3	0.0	2.091427593835559E-5	0.0	0.0	0.0
4	0.0	2.091427593835559E-5	0.0	0.0	0.0
5	0.0	6.274282781506677E-5	0.0	0.0	0.0
6	0.0	6.274282781506677E-5	0.0	0.0	0.0
7	0.0	6.274282781506677E-5	0.0	2.091427593835559E-5	0.0
8	0.0	6.274282781506677E-5	0.0	2.091427593835559E-5	0.0
9	0.0	1.4639993156848914E-4	0.0	2.091427593835559E-5	0.0
10-11	0.0	1.4639993156848914E-4	0.0	2.091427593835559E-5	0.0
12-13	0.0	1.6731420750684472E-4	0.0	2.091427593835559E-5	0.0
14-15	0.0	2.300570353219115E-4	0.0	2.091427593835559E-5	4.182855187671118E-5
16-17	0.0	2.7188558719862266E-4	0.0	3.1371413907533385E-5	4.182855187671118E-5
18-19	0.0	2.927998631369783E-4	0.0	5.2285689845888975E-5	4.182855187671118E-5
20-21	0.0	3.1371413907533386E-4	0.0	7.319996578424457E-5	5.2285689845888975E-5
22-23	0.0	3.4508555298286725E-4	0.0	1.2548565563013354E-4	6.274282781506677E-5
24-25	0.0	3.6599982892122283E-4	0.0	2.300570353219115E-4	6.274282781506677E-5
26-27	0.0	3.7645696689040065E-4	0.0	7.424567958116235E-4	8.365710375342236E-5
28-29	0.0	3.7645696689040065E-4	0.0	0.002049599041958848	8.365710375342236E-5
30-31	0.0	4.391997947054674E-4	0.0	0.006420682713075167	8.365710375342236E-5
32-33	0.0	4.391997947054674E-4	0.0	0.015100107227492738	9.411424172260015E-5
34-35	2.091427593835559E-5	4.914854845513563E-4	0.0	0.024856616952735618	1.0457137969177795E-4
36-37	2.091427593835559E-5	5.542283123664231E-4	0.0	0.0355542690952045	1.0457137969177795E-4
38-39	2.091427593835559E-5	5.751425883047788E-4	0.0	0.04653426396284119	1.0457137969177795E-4
40-41	2.091427593835559E-5	6.169711401814899E-4	0.0	0.055265974167104645	1.0457137969177795E-4
42-43	2.091427593835559E-5	6.692568300273789E-4	0.0	0.06191671391550173	1.1502851766095575E-4
44-45	2.091427593835559E-5	7.215425198732678E-4	0.0	0.06638191182834063	1.2548565563013354E-4
46-47	2.091427593835559E-5	7.319996578424457E-4	0.0	0.07012556722130629	1.4639993156848914E-4
48-49	2.091427593835559E-5	7.319996578424457E-4	0.0	0.07227973764295692	1.4639993156848914E-4
50-51	2.091427593835559E-5	7.842853476883346E-4	0.0	0.07494630782509726	1.4639993156848914E-4
52-53	2.091427593835559E-5	7.947424856575124E-4	0.0	0.07901413449510741	1.7777134547602253E-4
54-55	2.091427593835559E-5	8.051996236266902E-4	0.0	0.08351070382185387	2.091427593835559E-4
56-57	2.091427593835559E-5	8.15656761595868E-4	0.0	0.08680470228214487	2.091427593835559E-4
58-59	2.091427593835559E-5	8.574853134725792E-4	0.0	0.09036012919166533	2.091427593835559E-4
60-61	2.091427593835559E-5	8.574853134725792E-4	0.0	0.09350772772038785	2.091427593835559E-4
62-63	2.091427593835559E-5	8.574853134725792E-4	0.0	0.09542138396874739	2.091427593835559E-4
64-65	2.091427593835559E-5	0.001024799520979424	0.0	0.09760692580430554	2.091427593835559E-4
66-67	2.091427593835559E-5	0.001066628072856135	0.0	0.10136103833524036	2.091427593835559E-4
68-69	2.091427593835559E-5	0.001066628072856135	0.0	0.10427857982864097	2.091427593835559E-4
70-71	2.091427593835559E-5	0.001066628072856135	0.0	0.10595172190370941	2.091427593835559E-4
72-73	2.091427593835559E-5	0.001066628072856135	0.0	0.10805360663551417	2.091427593835559E-4
74-75	2.091427593835559E-5	0.0010875423487944906	0.0	0.1102914341609182	2.091427593835559E-4
76-77	2.091427593835559E-5	0.0011293709006712019	0.0	0.1120796047536476	2.091427593835559E-4
78-79	2.091427593835559E-5	0.0011502851766095575	0.0	0.11294754720508936	2.091427593835559E-4
80-81	2.091427593835559E-5	0.0011502851766095575	0.0	0.11359588975917839	2.091427593835559E-4
82-83	2.091427593835559E-5	0.0011502851766095575	0.0	0.11418148948545234	2.091427593835559E-4
84-85	2.091427593835559E-5	0.0011607423145787352	0.0	0.11458931786625028	2.091427593835559E-4
86-87	2.091427593835559E-5	0.0011711994525479131	0.0	0.11493440341923314	2.091427593835559E-4
88	2.091427593835559E-5	0.0011711994525479131	0.0	0.11496577483314067	2.091427593835559E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8795	0.0	54.07465	1
GTATCAA	16165	0.0	37.901943	1
ATCAACG	19215	0.0	31.421751	3
TCAACGC	19915	0.0	30.403452	4
AACGCAG	20090	0.0	30.094439	6
TATCAAC	20305	0.0	29.90181	2
ACGCAGA	20305	0.0	29.898855	7
CAACGCA	20270	0.0	29.812016	5
CGCAGAG	22495	0.0	27.72643	8
TGGTATC	3425	0.0	26.727531	2
GTGGTAT	3625	0.0	26.427183	1
GAGTACT	16940	0.0	23.97645	12-13
GCAGAGT	24660	0.0	23.830254	9
GTACATG	14670	0.0	23.10252	1
ACATGGG	14350	0.0	22.698862	3
TACATGG	14595	0.0	22.579681	2
CAGAGTA	23560	0.0	22.411194	10-11
GTACTTT	18620	0.0	21.28271	14-15
AGAGTAC	22175	0.0	21.246862	10-11
CATGGGG	10435	0.0	20.524813	4
>>END_MODULE