FastQCFastQC Report
Fri 27 May 2016
SRR2139418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8120165
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121000.051.4816061
GTATCAA223950.039.5805551
TCAACGC272000.031.9944934
ATCAACG272250.031.9655063
CAACGCA278550.031.22635
TATCAAC283000.030.9672582
AACGCAG283350.030.7435056
CGCAGAG319200.027.3463138
ACGCAGA323550.026.937217
GCAGAGT354850.023.43169
GTGGTAT54850.023.3023781
GTACATG235000.022.7764451
GAGTACT220500.022.7403612-13
TGGTATC53350.022.649292
TACATGG233000.022.3354022
CAGAGTA332600.021.55886510-11
AGAGTAC310200.021.3696910-11
ACATGGG246600.020.5727923
GTACTTT247150.020.19583514-15
AGTACTT239250.019.59782812-13
Produced by FastQC (version 0.11.5)