##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8120165
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68053543246966	33.0	33.0	33.0	33.0	33.0
2	32.69608548594764	33.0	33.0	33.0	33.0	33.0
3	32.68606697031403	33.0	33.0	33.0	33.0	33.0
4	36.615344146332	37.0	37.0	37.0	37.0	37.0
5	36.635589178298716	37.0	37.0	37.0	37.0	37.0
6	36.591745118479736	37.0	37.0	37.0	37.0	37.0
7	36.56739105670882	37.0	37.0	37.0	37.0	37.0
8	36.571566957075376	37.0	37.0	37.0	37.0	37.0
9	36.585190448716254	37.0	37.0	37.0	37.0	37.0
10-11	36.53169486088029	37.0	37.0	37.0	37.0	37.0
12-13	36.494163357517984	37.0	37.0	37.0	37.0	37.0
14-15	35.4361528367958	40.0	37.0	40.0	19.5	40.0
16-17	35.72077390053035	40.0	37.0	40.0	24.0	40.0
18-19	38.06220396999322	40.0	37.0	40.0	35.0	40.0
20-21	38.39897871272559	40.0	37.0	40.0	37.0	40.0
22-23	38.43479202670482	40.0	37.0	40.0	37.0	40.0
24-25	38.46792949517152	40.0	37.0	40.0	37.0	40.0
26-27	38.407397263395566	40.0	37.0	40.0	37.0	40.0
28-29	38.33663524440015	40.0	37.0	40.0	37.0	40.0
30-31	38.213887429622716	40.0	37.0	40.0	37.0	40.0
32-33	38.02542753791033	40.0	37.0	40.0	35.0	40.0
34-35	37.94332429748417	40.0	37.0	40.0	33.0	40.0
36-37	37.87472973417989	40.0	37.0	40.0	33.0	40.0
38-39	37.87788410944653	40.0	37.0	40.0	33.0	40.0
40-41	37.82298411682833	40.0	37.0	40.0	33.0	40.0
42-43	37.77589084209987	40.0	37.0	40.0	33.0	40.0
44-45	37.72940618749978	40.0	37.0	40.0	33.0	40.0
46-47	37.58940935662628	40.0	37.0	40.0	33.0	40.0
48-49	37.366384671843974	40.0	37.0	40.0	33.0	40.0
50-51	37.27832776857704	40.0	37.0	40.0	33.0	40.0
52-53	37.20177618647327	40.0	37.0	40.0	33.0	40.0
54-55	36.99048131038485	37.0	37.0	40.0	33.0	40.0
56-57	36.88695510611277	37.0	37.0	40.0	33.0	40.0
58-59	36.83649503172642	37.0	37.0	40.0	33.0	40.0
60-61	36.720751248228005	37.0	37.0	40.0	33.0	40.0
62-63	36.54370779328145	37.0	37.0	40.0	33.0	40.0
64-65	36.27590031035783	37.0	37.0	40.0	33.0	40.0
66-67	35.948521291293616	37.0	35.0	40.0	33.0	40.0
68-69	35.6574695793317	37.0	33.0	38.5	33.0	40.0
70-71	35.41523543844957	37.0	33.0	37.0	30.0	40.0
72-73	35.1975498167735	37.0	33.0	37.0	30.0	40.0
74-75	34.916901626414074	37.0	33.0	37.0	27.0	40.0
76-77	32.05870159532726	33.0	30.0	35.0	24.5	37.0
78-79	34.18895500848322	37.0	33.0	37.0	27.0	37.0
80-81	34.513830894235426	37.0	33.0	37.0	27.0	37.0
82-83	34.483407934312325	37.0	33.0	37.0	27.0	37.0
84-85	34.44210286685343	37.0	33.0	37.0	27.0	37.0
86-87	34.265510867771596	37.0	33.0	37.0	27.0	37.0
88-89	34.03735141187226	37.0	33.0	37.0	27.0	37.0
90-91	33.88081562414037	37.0	33.0	37.0	27.0	37.0
92-93	33.898414153023154	37.0	33.0	37.0	27.0	37.0
94-95	33.831905537220834	37.0	33.0	37.0	27.0	37.0
96-97	33.779829041284664	33.0	33.0	37.0	27.0	37.0
98-99	33.780348120276976	33.0	33.0	37.0	27.0	37.0
100	33.82365844566925	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	9.0
20	65.0
21	331.0
22	1224.0
23	3711.0
24	8475.0
25	17685.0
26	31545.0
27	50976.0
28	76281.0
29	108057.0
30	147465.0
31	198739.0
32	264117.0
33	359659.0
34	506067.0
35	782031.0
36	1399353.0
37	2544928.0
38	1595477.0
39	23966.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22673992304091	19.845574433403883	14.23818354273402	23.68950210082119
2	14.668681412736003	20.89146356031246	41.81808665624811	22.621768370703425
3	18.275743035956392	26.061452228445926	30.333357101715496	25.329447633882186
4	12.427418069701805	18.020022822283472	38.66942343562311	30.883135672391614
5	13.392079316683247	37.690941481680525	35.33069184121787	13.586287360418355
6	29.98394737052757	37.77228664688464	18.676492411176373	13.567273571411418
7	26.462627298829517	32.79189523858197	22.86973232686774	17.875745135720763
8	24.460537439818033	36.736507201516226	20.97853922919054	17.8244161294752
9	25.720856657469398	16.90634365188392	21.047527975108878	36.3252717155378
10-11	24.31797876028381	26.817176744561227	28.580681550190175	20.284162944964788
12-13	25.299996982819927	24.390255616727	28.77623176376342	21.53351563668965
14-15	22.503454334496475	25.364142288297227	26.73157566226771	25.400827714938597
16-17	21.767501029843604	28.693875062883574	27.32941387274766	22.20921003452516
18-19	21.693112147351687	27.675392064077514	29.38549278247425	21.24600300609655
20-21	22.507097033775175	26.881576814010206	29.394525001984356	21.216801150230264
22-23	22.19222687665352	26.870156147554763	29.221804515445697	21.715812460346015
24-25	21.961373476494934	27.011766010569232	29.338161520416854	21.68869899251898
26-27	21.93050649113499	27.19837408137657	29.258144728063545	21.612974699424896
28-29	21.825360869013032	27.241740742520363	29.29511705386082	21.637781334605783
30-31	22.158879470810167	27.089212599407432	29.18157158008118	21.57033634970123
32-33	21.929936541031072	27.24748168700749	29.12365882263326	21.698922949328182
34-35	22.06222288580029	27.314319961622154	29.094665053494058	21.528792099083503
36-37	22.085075353179974	27.241470668204933	28.93991205854678	21.733541920068316
38-39	21.88148695492921	27.3785483244535	29.0369234166389	21.70304130397839
40-41	22.21688587022862	27.249292657593827	28.82055278071496	21.7132686914626
42-43	22.128282203054283	27.329662495050695	28.618931150794648	21.923124151100374
44-45	22.202898328219597	27.52676885413077	28.35122307140389	21.91910974624574
46-47	22.307943271670343	27.48739263626731	27.953090891426385	22.25157320063596
48-49	22.2387773729472	27.677741948074917	27.879355543398066	22.20412513557982
50-51	22.27739821013274	27.843512974357687	27.738454965522934	22.140633849986635
52-53	22.38192480451971	27.769207499380826	27.627985825556767	22.2208818705427
54-55	22.35501756314878	27.665044872745913	27.664402051986713	22.315535512118586
56-57	22.443694825457282	27.86298683932301	27.501616847049558	22.19170148817015
58-59	22.316406839418246	27.900679826849416	27.61722248768474	22.165690846047596
60-61	22.411989037155088	27.871998767838363	27.592295465573386	22.123716729433163
62-63	22.320542082019127	28.02660134309482	27.512329592640967	22.140526982245085
64-65	22.30902516668142	27.931156023156863	27.49826975157154	22.261549058590177
66-67	22.384744834583024	27.89357004479993	27.414688838645425	22.30699628197162
68-69	22.340554980853295	27.90147593385925	27.449394887631918	22.30857419765554
70-71	22.366631775060615	27.723077484055743	27.46783212120939	22.44245861967425
72-73	22.421709115060715	27.702855672529115	27.41267460833842	22.462760604071747
74-75	22.292123211359883	27.779152803465312	27.443532171136326	22.485191814038483
76-77	22.41031523618496	27.701682389585947	27.37753462451679	22.510467749712298
78-79	22.38646032166515	27.759725478513158	27.3136308132186	22.54018338660309
80-81	22.381115089908988	27.740281637640646	27.344430413610876	22.53417285883949
82-83	22.447063846381646	27.720151787804028	27.417490059810312	22.415294306004018
84-85	22.543819923556647	27.697038147486875	27.347825970664573	22.411315958291905
86-87	22.332264871601588	27.773344645061304	27.403344612305336	22.491045871031776
88-89	22.4177176337796	27.679989587131796	27.428989823354982	22.473302955733622
90-91	22.481965216293666	27.637849330352076	27.453705120614824	22.426480332739434
92-93	22.477379469131588	27.618435235423565	27.48019111934907	22.42399417609578
94-95	22.341055432391293	27.67070952450054	27.510970916875245	22.477264126232917
96-97	22.344773017123927	27.796585563754444	27.60607006412206	22.25257135499956
98-99	22.558461720381306	28.53348985087957	26.67477640369848	22.233272025040645
100	20.65901705432536	28.198212796346795	27.822990196286618	23.319779953041227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1454.0
1	1414.0
2	2257.5
3	3397.5
4	4890.5
5	8513.5
6	11541.0
7	13313.0
8	14402.0
9	14103.5
10	13080.5
11	11955.0
12	12839.0
13	17314.0
14	27811.5
15	41954.0
16	54284.0
17	62262.0
18	62837.0
19	57231.0
20	49585.5
21	41722.0
22	36122.0
23	35127.0
24	38304.5
25	45476.0
26	56746.0
27	70213.0
28	85175.5
29	101312.5
30	117236.0
31	133675.5
32	151664.0
33	169946.0
34	187943.5
35	204584.0
36	223692.0
37	241724.0
38	252391.5
39	261622.0
40	273513.5
41	283841.0
42	290768.5
43	297524.5
44	303669.0
45	310103.5
46	314251.0
47	316824.5
48	318913.0
49	316659.0
50	310350.0
51	301689.0
52	290327.5
53	275370.0
54	259300.5
55	241988.0
56	222857.5
57	202139.0
58	180287.0
59	157421.0
60	131759.0
61	106547.5
62	84241.5
63	64973.0
64	49075.5
65	37042.5
66	28153.0
67	21383.5
68	16323.0
69	12042.0
70	8874.0
71	6611.5
72	4879.5
73	3540.0
74	2504.5
75	1727.0
76	1218.0
77	837.0
78	557.0
79	408.0
80	291.0
81	194.5
82	143.5
83	102.5
84	75.0
85	61.5
86	50.0
87	40.0
88	26.5
89	14.0
90	7.5
91	5.5
92	5.0
93	4.5
94	3.5
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	8.620514484619463E-5
2	4.310257242309732E-4
3	0.0016502127727700113
4	3.817656414617191E-4
5	8.620514484619463E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.080330264224926
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008501486682082286
26-27	0.023661633828727862
28-29	0.008770336526200457
30-31	1.296838486856264E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.02784244796954452
50-51	0.07235452454025885
52-53	0.060746565558976603
54-55	0.04068684953730596
56-57	0.00524407627402511
58-59	0.009336607456798252
60-61	0.03481210628835423
62-63	0.03561025169097926
64-65	0.0
66-67	0.003276448266407345
68-69	0.03421427635770124
70-71	0.08867187849210217
72-73	0.07986785340866158
74-75	0.04003050358441646
76-77	0.009014900205191733
78-79	0.0012309797298900376
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0017938179760915549
92-93	0.0034647193060628203
94-95	0.0012029618282053997
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	245.0
20-21	808.0
22-23	1894.0
24-25	3802.0
26-27	4784.0
28-29	8192.0
30-31	16121.0
32-33	15971.0
34-35	17226.0
36-37	17165.0
38-39	20089.0
40-41	25441.0
42-43	32372.0
44-45	40406.0
46-47	40011.0
48-49	30061.0
50-51	29380.0
52-53	27230.0
54-55	28822.0
56-57	35217.0
58-59	51201.0
60-61	33478.0
62-63	35573.0
64-65	45413.0
66-67	62204.0
68-69	47174.0
70-71	41012.0
72-73	58231.0
74-75	44964.0
76-77	25049.0
78-79	38069.0
80-81	61577.0
82-83	59598.0
84-85	78173.0
86-87	89315.0
88-89	98627.0
90-91	103560.0
92-93	111786.0
94-95	133277.0
96-97	322260.0
98-99	62421.0
100-101	6121966.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.79850643616767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.76182724721339	78.77991046475589
2	6.58751393457311	11.435727412967763
3	1.554407206568779	4.047606717713567
4	0.5136159882471353	1.783244026463503
5	0.23030521133047835	0.9995074183975738
6	0.1187460459457764	0.6184187659976359
7	0.07004255669723905	0.425571251580366
8	0.042000633013663496	0.29164737719676703
9	0.02630674113148637	0.2055047255474325
>10	0.09300151299998723	1.2168750834194912
>50	0.001732235347896155	0.09924325914218719
>100	4.7925023761782745E-4	0.07746692494877408
>500	0.0	0.0
>1k	2.1436693288006566E-5	0.01927657186901041
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.4630041384627038E-5	0.0	0.0	0.0
3	0.0	2.4630041384627038E-5	0.0	0.0	0.0
4	0.0	2.4630041384627038E-5	0.0	0.0	0.0
5	0.0	2.4630041384627038E-5	0.0	0.0	0.0
6	1.2315020692313519E-5	2.4630041384627038E-5	0.0	0.0	1.2315020692313519E-5
7	1.2315020692313519E-5	2.4630041384627038E-5	0.0	0.0	1.2315020692313519E-5
8	1.2315020692313519E-5	3.694506207694055E-5	0.0	0.0	1.2315020692313519E-5
9	1.2315020692313519E-5	8.620514484619463E-5	0.0	1.2315020692313519E-5	1.2315020692313519E-5
10-11	1.2315020692313519E-5	8.620514484619463E-5	0.0	1.2315020692313519E-5	1.2315020692313519E-5
12-13	1.2315020692313519E-5	8.620514484619463E-5	0.0	2.4630041384627038E-5	1.2315020692313519E-5
14-15	1.2315020692313519E-5	1.231502069231352E-4	0.0	3.07875517307838E-5	3.694506207694056E-5
16-17	1.2315020692313519E-5	1.231502069231352E-4	0.0	7.389012415388112E-5	7.38901241538811E-5
18-19	1.2315020692313519E-5	1.231502069231352E-4	0.0	1.231502069231352E-4	8.620514484619463E-5
20-21	2.4630041384627035E-5	1.231502069231352E-4	0.0	1.7241028969238926E-4	9.852016553850815E-5
22-23	3.694506207694055E-5	1.6009526900007573E-4	0.0	2.9556049661552447E-4	1.2930771726929195E-4
24-25	3.694506207694055E-5	1.7856780003854602E-4	0.0	7.696887932695949E-4	1.7856780003854602E-4
26-27	3.694506207694055E-5	1.8472531038470278E-4	0.0	0.0020073483728471034	1.8472531038470278E-4
28-29	4.3102572423097314E-5	1.8472531038470278E-4	0.0	0.007820038139619085	1.8472531038470278E-4
30-31	4.9260082769254076E-5	2.463004138462704E-4	0.0	0.011957885092236427	1.8472531038470278E-4
32-33	4.9260082769254076E-5	2.586154345385839E-4	0.0	0.021360403390817795	1.970403310770163E-4
34-35	4.9260082769254076E-5	3.386630690386217E-4	0.0	0.031329412641245585	2.093553517693298E-4
36-37	4.9260082769254076E-5	4.063956828463461E-4	0.0	0.03748076547705619	2.1551286211548656E-4
38-39	4.9260082769254076E-5	4.187107035386596E-4	0.0	0.04449416976132874	2.278278828078001E-4
40-41	4.9260082769254076E-5	4.802858070002272E-4	0.0	0.051021130728254906	2.401429035001136E-4
42-43	5.541759311541083E-5	5.172308690771677E-4	0.0	0.056895395598488455	2.463004138462704E-4
44-45	6.15751034615676E-5	6.219085449618326E-4	0.0	0.06230168968241409	2.524579241924271E-4
46-47	6.15751034615676E-5	7.142712001541841E-4	0.0	0.06780034642153207	2.709304552308974E-4
48-49	1.046776758846649E-4	7.142712001541841E-4	0.0	0.0736930838228041	2.709304552308974E-4
50-51	1.231502069231352E-4	8.18948876038849E-4	0.0	0.07995527184484552	2.709304552308974E-4
52-53	1.231502069231352E-4	8.312638967311625E-4	0.0	0.10404960982935692	2.832454759232109E-4
54-55	1.2930771726929195E-4	8.497364277696327E-4	0.0	0.127306526406791	2.955604966155244E-4
56-57	1.354652276154487E-4	8.620514484619463E-4	0.0	0.15031714257037881	2.955604966155244E-4
58-59	1.354652276154487E-4	8.620514484619463E-4	0.0	0.17130193783008102	2.955604966155244E-4
60-61	1.354652276154487E-4	8.9283900019273E-4	0.0	0.18713905444039625	2.955604966155244E-4
62-63	1.6009526900007573E-4	9.420990829619841E-4	0.0	0.1936475428762839	2.955604966155244E-4
64-65	1.6009526900007573E-4	0.0010837218209235897	0.0	0.19596276676643887	2.955604966155244E-4
66-67	1.7241028969238926E-4	0.00112066688300053	0.0	0.19877059148428633	2.955604966155244E-4
68-69	1.8472531038470278E-4	0.001151454434731314	0.0	0.2022680573609034	2.955604966155244E-4
70-71	1.9704033107701628E-4	0.0011699269657697842	0.0	0.20446628855448135	3.2634804834630825E-4
72-73	2.3398539315395685E-4	0.0011883994968082544	0.0	0.20627043908590526	3.448205793847785E-4
74-75	2.3398539315395685E-4	0.0012068720278467248	0.0	0.20815463725182926	3.448205793847785E-4
76-77	2.3398539315395685E-4	0.001225344558885195	0.0	0.20958317965213763	3.448205793847785E-4
78-79	2.3398539315395685E-4	0.0012315020692313518	0.0	0.21005730794879168	3.448205793847785E-4
80-81	2.3398539315395685E-4	0.0012315020692313518	0.0	0.21040828603852263	3.5713560007709204E-4
82-83	2.3398539315395685E-4	0.0012315020692313518	0.0	0.21083931176275358	4.063956828463461E-4
84-85	2.3398539315395685E-4	0.0012438170899236654	0.0	0.21115950230075375	4.063956828463461E-4
86-87	2.463004138462704E-4	0.001299234683039076	0.0	0.21136885765252306	4.063956828463461E-4
88	2.586154345385839E-4	0.00133002223476986	0.0	0.21139964520425386	4.063956828463461E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12100	0.0	51.481606	1
GTATCAA	22395	0.0	39.580555	1
TCAACGC	27200	0.0	31.994493	4
ATCAACG	27225	0.0	31.965506	3
CAACGCA	27855	0.0	31.2263	5
TATCAAC	28300	0.0	30.967258	2
AACGCAG	28335	0.0	30.743505	6
CGCAGAG	31920	0.0	27.346313	8
ACGCAGA	32355	0.0	26.93721	7
GCAGAGT	35485	0.0	23.4316	9
GTGGTAT	5485	0.0	23.302378	1
GTACATG	23500	0.0	22.776445	1
GAGTACT	22050	0.0	22.74036	12-13
TGGTATC	5335	0.0	22.64929	2
TACATGG	23300	0.0	22.335402	2
CAGAGTA	33260	0.0	21.558865	10-11
AGAGTAC	31020	0.0	21.36969	10-11
ACATGGG	24660	0.0	20.572792	3
GTACTTT	24715	0.0	20.195835	14-15
AGTACTT	23925	0.0	19.597828	12-13
>>END_MODULE