FastQCFastQC Report
Fri 27 May 2016
SRR2139417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7523377
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151750.047.735681
GTATCAA268750.036.896831
ATCAACG326500.030.0993583
ACGCAGA340850.029.0265777
AACGCAG344550.028.5625926
TCAACGC345000.028.5101814
TATCAAC347650.028.3890632
CAACGCA349800.028.0981885
CGCAGAG381200.026.6090018
GCAGAGT403650.023.3817549
TACATGG263300.023.0545562
GAGTACT258450.022.81369212-13
GTACATG281850.022.4211711
ACATGGG263450.022.3368533
AGACCGA49600.021.15373888-89
CAGAGTA390900.021.08545310-11
AGAGTAC373850.020.8589610-11
ACCGAGG52150.020.79992590-91
CATGGGG185650.020.2830434
AGTACTT279000.020.15931712-13
Produced by FastQC (version 0.11.5)