##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139417_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7523377
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73308967502227	33.0	33.0	33.0	33.0	33.0
2	32.78227290749885	33.0	33.0	33.0	33.0	33.0
3	33.4451518247723	33.0	33.0	33.0	33.0	37.0
4	36.57807644093869	37.0	37.0	37.0	37.0	37.0
5	36.59614797982342	37.0	37.0	37.0	37.0	37.0
6	36.5539037854942	37.0	37.0	37.0	37.0	37.0
7	36.538322218865275	37.0	37.0	37.0	37.0	37.0
8	36.52673779341378	37.0	37.0	37.0	37.0	37.0
9	36.55290436728081	37.0	37.0	37.0	37.0	37.0
10-11	36.501732798981095	37.0	37.0	37.0	37.0	37.0
12-13	36.56943770064959	37.0	37.0	37.0	37.0	38.5
14-15	34.7130820374946	40.0	35.0	40.0	17.5	40.0
16-17	35.75154760953758	40.0	35.0	40.0	24.0	40.0
18-19	37.980303592389426	40.0	37.0	40.0	35.0	40.0
20-21	38.30892329750441	40.0	37.0	40.0	37.0	40.0
22-23	38.359000785804355	40.0	37.0	40.0	37.0	40.0
24-25	38.38071185499511	40.0	37.0	40.0	37.0	40.0
26-27	38.309988159599015	40.0	37.0	40.0	37.0	40.0
28-29	38.22828280809276	40.0	37.0	40.0	37.0	40.0
30-31	38.07724192778767	40.0	37.0	40.0	35.0	40.0
32-33	37.875161754290275	40.0	37.0	40.0	33.0	40.0
34-35	37.788268123365526	40.0	37.0	40.0	33.0	40.0
36-37	37.7305538246935	40.0	37.0	40.0	33.0	40.0
38-39	37.725076047425745	40.0	37.0	40.0	33.0	40.0
40-41	37.68733171009005	40.0	37.0	40.0	33.0	40.0
42-43	37.64654887639429	40.0	37.0	40.0	33.0	40.0
44-45	37.58937977724683	40.0	37.0	40.0	33.0	40.0
46-47	37.432861193099995	40.0	37.0	40.0	33.0	40.0
48-49	37.22407218573426	40.0	37.0	40.0	33.0	40.0
50-51	37.13543339109441	40.0	37.0	40.0	33.0	40.0
52-53	37.059975770165615	37.0	37.0	40.0	33.0	40.0
54-55	36.843899151954474	37.0	37.0	40.0	33.0	40.0
56-57	36.751797446259886	37.0	37.0	40.0	33.0	40.0
58-59	36.69774994130752	37.0	37.0	40.0	33.0	40.0
60-61	36.580196707923484	37.0	37.0	40.0	33.0	40.0
62-63	36.39639147817428	37.0	37.0	40.0	33.0	40.0
64-65	36.11809665320647	37.0	37.0	40.0	33.0	40.0
66-67	35.78883564037269	37.0	33.0	40.0	33.0	40.0
68-69	35.50141511525051	37.0	33.0	37.0	30.0	40.0
70-71	35.27427253384104	37.0	33.0	37.0	27.0	40.0
72-73	35.04338340261074	37.0	33.0	37.0	27.0	40.0
74-75	34.38900764013412	37.0	33.0	37.0	27.0	38.5
76-77	32.19179831737937	33.0	30.0	37.0	24.5	37.0
78-79	34.09245679197444	37.0	33.0	37.0	27.0	37.0
80-81	34.380258317558344	37.0	33.0	37.0	27.0	37.0
82-83	34.35442992188604	37.0	33.0	37.0	27.0	37.0
84-85	34.308572138095116	37.0	33.0	37.0	27.0	37.0
86-87	34.11580493752513	37.0	33.0	37.0	27.0	37.0
88-89	33.890104627464176	37.0	33.0	37.0	27.0	37.0
90-91	33.74726976225479	37.0	33.0	37.0	27.0	37.0
92-93	33.76062695792478	35.0	33.0	37.0	27.0	37.0
94-95	33.688973679564945	33.0	33.0	37.0	27.0	37.0
96-97	33.63263542239038	33.0	33.0	37.0	27.0	37.0
98-99	33.633104730454335	33.0	33.0	37.0	27.0	37.0
100	33.600744857963114	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	17.0
20	76.0
21	322.0
22	1262.0
23	3561.0
24	8425.0
25	17084.0
26	29917.0
27	48620.0
28	73514.0
29	103853.0
30	142027.0
31	190959.0
32	255612.0
33	348915.0
34	493277.0
35	765986.0
36	1336338.0
37	2283590.0
38	1384749.0
39	35270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.250027580754455	0.0	24.369277990345275	29.380694428900274
2	15.046974400908745	23.93532511368269	38.88719564268751	22.13050484272106
3	16.531650704716597	25.216766347994792	32.97768747471192	25.27389547257669
4	12.699344401010773	23.419224603298343	37.566075958991135	26.315355036699746
5	16.98162412884483	36.69949160905045	32.32166440880545	13.997219853299278
6	27.534077239527605	37.78772754158719	20.229153236257787	14.44904198262742
7	25.62754857495133	33.198459403970745	22.691651374220058	18.482340646857864
8	24.127303388305084	32.17731429439586	22.23857582006027	21.456806497238784
9	24.15461447931972	20.566127369885425	23.34374194995942	31.935516200835433
10-11	24.750544134713977	26.26003733887069	28.404028142201426	20.58539038421391
12-13	25.32883253557406	24.3351332438308	28.35662477742063	21.979409443174507
14-15	22.80918823114409	26.191085493253517	26.954352684905857	24.04537359069653
16-17	21.264102627324313	28.850417096632075	27.80904292108736	22.076437354956248
18-19	21.201499167395063	27.628234983868943	30.05599480871354	21.11427104002246
20-21	22.318109789920975	26.999263983678702	29.97809630315631	20.70452992324401
22-23	21.950620745604684	26.78813482762451	29.877647049125915	21.38359737764489
24-25	21.63102597211205	27.00945763258322	30.1455487970002	21.213967598304524
26-27	21.60400961141849	27.381994522401865	29.89709239017951	21.116903476000136
28-29	21.395975514482913	27.360620569725846	29.969949229382888	21.273454686408353
30-31	21.831882961239305	27.207173119600046	29.898166981654605	21.06277693750604
32-33	21.64983871687086	27.519714146958652	29.69299269419955	21.137454441970938
34-35	21.576237430444227	27.46297784695313	29.722582861291563	21.238201861311076
36-37	21.767948211502528	27.511192909300703	29.554514406144783	21.166344473051986
38-39	21.496175856583818	27.718004531717977	29.605459666899826	21.180359944798383
40-41	21.893977089538986	27.47368035717433	29.315275764777983	21.317066788508704
42-43	21.951512984060788	27.439319649544448	29.188025217889436	21.421142148505336
44-45	21.802916861751136	28.2278321115668	28.565473550665438	21.403777476016625
46-47	22.091729191563218	27.756393053079126	28.02625405025239	22.125623705105266
48-49	22.065599272659483	27.910783960665924	28.127052055965308	21.89656471070928
50-51	21.829738078869962	28.225318632320544	27.854293294699513	22.090649994109985
52-53	22.15332529896154	28.024936735688883	27.780000639505058	22.041737325844522
54-55	22.113474083173415	28.085239072948763	27.714507751981877	22.08677909189594
56-57	22.299546324427755	28.389276425496195	27.3639878426217	21.94718940745435
58-59	22.107259302768302	28.181381704788272	27.766261691996487	21.945097300446943
60-61	22.21683394480992	28.23848357490669	27.615027564795408	21.92965491548798
62-63	22.0390202982483	28.433985911333558	27.49420845399207	22.03278533642607
64-65	22.004854792999485	28.20985198971276	27.50333948090491	22.28195373638285
66-67	22.094661619792124	28.264571834214564	27.49008139138584	22.150685154607473
68-69	22.198652482665537	28.369212143200922	27.548961562266495	21.883173811867046
70-71	22.22387478902829	27.809633433210074	27.43457261896294	22.531919158798694
72-73	22.197969828103574	27.95255022461089	27.45484839983987	22.394631547445666
74-75	22.008010439946236	28.07334910364955	27.678804352334385	22.23983610406982
76-77	22.2036172319446	27.87121545008388	27.299809197947855	22.62535812002367
78-79	22.174433878869493	27.96191672144846	27.27883007337416	22.58481932630789
80-81	22.194423742038584	28.12883597555409	27.32147395616137	22.355266326245953
82-83	22.472397020241058	27.689528539913606	27.403124165321785	22.43495027452355
84-85	22.533373223859627	27.720626927606855	27.349770711082904	22.39622913745061
86-87	22.048676939036564	28.02922562665059	27.321772021046105	22.600325413266738
88-89	22.527842153845768	27.819293287198043	27.384741185819067	22.268123373137122
90-91	22.489999429501236	27.865683953404663	27.45512950449922	22.189187112594883
92-93	22.2559615568731	27.71477006414588	27.489130374454874	22.54013800452615
94-95	22.537503181383332	27.695014155497706	27.380545798920302	22.386936864198663
96-97	22.359439094436702	27.996787200135337	27.748143397627008	21.895630307800957
98-99	23.276800451245414	30.185812217257364	27.241799137017637	19.295588194479592
100	22.674357327320635	30.343221949844658	21.57778273009714	25.404637992737573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1534.0
1	1500.5
2	2886.5
3	4587.5
4	7100.5
5	13317.0
6	17954.5
7	19704.5
8	19840.5
9	17888.0
10	15398.0
11	13114.5
12	14159.5
13	19827.0
14	34158.5
15	55908.5
16	76097.5
17	88751.0
18	88493.5
19	78430.5
20	66141.0
21	54230.0
22	46004.5
23	43979.0
24	46672.5
25	54135.0
26	68032.5
27	83813.0
28	96139.5
29	109461.5
30	120956.0
31	130163.0
32	145885.0
33	163233.0
34	180790.5
35	198599.5
36	218843.0
37	237167.5
38	244189.0
39	249457.0
40	258124.0
41	262519.0
42	263681.5
43	268634.5
44	275586.0
45	281792.0
46	286484.5
47	290568.5
48	292914.0
49	293069.5
50	290534.5
51	283880.0
52	273446.5
53	259403.0
54	245097.5
55	230089.5
56	210402.5
57	189694.0
58	168822.0
59	147864.5
60	123737.5
61	96183.0
62	73056.5
63	54382.0
64	39611.0
65	29243.0
66	21660.5
67	16252.5
68	12196.0
69	8927.5
70	6893.5
71	5177.5
72	3786.0
73	2745.0
74	1966.5
75	1403.0
76	1000.5
77	727.0
78	501.0
79	334.0
80	232.5
81	153.5
82	112.0
83	79.5
84	60.5
85	46.0
86	32.0
87	21.5
88	15.0
89	13.5
90	13.5
91	10.5
92	7.5
93	6.0
94	4.5
95	3.5
96	3.0
97	3.0
98	2.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.9937855035046095E-4
2	3.1900568056073757E-4
3	9.171413316121204E-4
4	3.7217329398752714E-4
5	4.386328107710142E-4
6	3.588813906308298E-4
7	7.310546846183569E-4
8	7.70930394688449E-4
9	0.0016349041128737802
10-11	0.00868625884360175
12-13	0.24176643015496896
14-15	7.494267534379841
16-17	6.6459516783486996E-6
18-19	1.3291903356697399E-5
20-21	6.646345244536654E-6
22-23	3.3237677513295235E-4
24-25	0.011071649933181096
26-27	0.02266179630997292
28-29	0.007338989105530402
30-31	6.0037903930014486E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.0451576257519704E-5
44-45	4.119083811410219E-5
46-47	0.0016136686181206816
48-49	0.030392107076890492
50-51	0.07309526433204099
52-53	0.05431426508104647
54-55	0.03254618232782885
56-57	0.003928703776523859
58-59	0.013045400879616262
60-61	0.03874422770239171
62-63	0.032000783740551375
64-65	3.843141300924024E-4
66-67	0.007036999076972817
68-69	0.04430599345291074
70-71	0.0924396145932372
72-73	0.07834523206341161
74-75	0.03672602555430802
76-77	0.007475288196965173
78-79	7.238618786560434E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	1.5951153872889788E-4
90-91	0.0021318632459186968
92-93	0.003382009409617359
94-95	7.958922057745693E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	260.0
20-21	1052.0
22-23	2101.0
24-25	3780.0
26-27	6010.0
28-29	10857.0
30-31	16947.0
32-33	19343.0
34-35	24677.0
36-37	26188.0
38-39	28101.0
40-41	37634.0
42-43	49152.0
44-45	60582.0
46-47	65383.0
48-49	46964.0
50-51	41122.0
52-53	48977.0
54-55	46126.0
56-57	47741.0
58-59	42175.0
60-61	67227.0
62-63	49696.0
64-65	65551.0
66-67	74811.0
68-69	46392.0
70-71	60202.0
72-73	70126.0
74-75	52067.0
76-77	42614.0
78-79	61498.0
80-81	81379.0
82-83	78674.0
84-85	84830.0
86-87	85605.0
88-89	89861.0
90-91	96529.0
92-93	106373.0
94-95	138956.0
96-97	406815.0
98-99	1324849.0
100-101	3814150.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.25124308628128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.62750693462995	72.89722617011327
2	7.532872457592056	12.391762472947143
3	2.0850915205003893	5.145041085294641
4	0.7837337506155774	2.578523009472192
5	0.36426196677181194	1.4980499788017607
6	0.19879238094166835	0.9810552269120273
7	0.11288740283063257	0.6499590448120922
8	0.07587406984251212	0.4992589250049558
9	0.05037384703455795	0.3728980383867518
>10	0.16223844029209372	2.089013381190715
>50	0.0038455681383016997	0.2104015024237552
>100	0.002032644315568335	0.3097530682403242
>500	2.4450828780502357E-4	0.13610193185226543
>1k	2.445082071025047E-4	0.2409561645481217
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.3291903356697397E-5	0.0
3	0.0	0.0	0.0	1.3291903356697397E-5	0.0
4	0.0	0.0	0.0	2.6583806713394795E-5	0.0
5	0.0	2.6583806713394795E-5	0.0	2.6583806713394795E-5	0.0
6	0.0	3.9875710070092196E-5	0.0	6.645951678348698E-5	0.0
7	0.0	3.9875710070092196E-5	0.0	6.645951678348698E-5	0.0
8	0.0	3.9875710070092196E-5	0.0	6.645951678348698E-5	0.0
9	0.0	3.9875710070092196E-5	0.0	6.645951678348698E-5	0.0
10-11	6.645951678348699E-6	3.9875710070092196E-5	0.0	1.0633522685357918E-4	0.0
12-13	1.3291903356697397E-5	3.9875710070092196E-5	0.0	1.1298117853192788E-4	0.0
14-15	1.3291903356697397E-5	7.310546846183569E-5	0.0	1.4621093692367138E-4	0.0
16-17	1.3291903356697397E-5	1.1298117853192788E-4	0.0	1.661487919587175E-4	0.0
18-19	1.3291903356697397E-5	1.5285688860202008E-4	0.0	2.8577592216899405E-4	0.0
20-21	1.3291903356697397E-5	1.7279474363706616E-4	0.0	4.253409074143167E-4	0.0
22-23	1.3291903356697397E-5	1.7279474363706616E-4	0.0	7.842222980451465E-4	0.0
24-25	1.3291903356697397E-5	1.8608664699376357E-4	0.0	0.0016415500645521287	0.0
26-27	1.3291903356697397E-5	1.8608664699376357E-4	0.0	0.0034625408244196723	0.0
28-29	1.3291903356697397E-5	1.8608664699376357E-4	0.0	0.007975142014018438	0.0
30-31	1.3291903356697397E-5	1.9937855035046095E-4	0.0	0.010480665796755897	0.0
32-33	1.3291903356697397E-5	2.1931640538550706E-4	0.0	0.014062833751385846	0.0
34-35	1.3291903356697397E-5	2.5254616377725053E-4	0.0	0.018223199502032134	0.0
36-37	2.6583806713394795E-5	2.7912997049064534E-4	0.0	0.02346020942457091	0.0
38-39	2.6583806713394795E-5	3.1235972888238886E-4	0.0	0.030930259111034846	0.0
40-41	2.6583806713394795E-5	3.2565163223908627E-4	0.0	0.03880571184987805	6.645951678348699E-6
42-43	2.6583806713394795E-5	3.32297583917435E-4	0.0	0.04794389540760752	1.3291903356697397E-5
44-45	2.6583806713394795E-5	3.389435355957836E-4	0.0	0.0579859283935924	1.3291903356697397E-5
46-47	2.6583806713394795E-5	3.5888139063082973E-4	0.0	0.0678950423460103	1.3291903356697397E-5
48-49	2.6583806713394795E-5	3.7217329398752714E-4	0.0	0.08066191552011816	1.3291903356697397E-5
50-51	2.6583806713394795E-5	5.183842309111986E-4	0.0	0.09320282633716215	1.3291903356697397E-5
52-53	2.6583806713394795E-5	5.582599409812907E-4	0.0	0.12255134894875001	1.3291903356697397E-5
54-55	2.6583806713394795E-5	5.582599409812907E-4	0.0	0.16389581433975725	1.3291903356697397E-5
56-57	2.6583806713394795E-5	6.114275544080803E-4	0.0	0.20585835323685095	1.3291903356697397E-5
58-59	2.6583806713394795E-5	6.180735060864291E-4	0.0	0.38011520624315387	1.3291903356697397E-5
60-61	2.6583806713394795E-5	6.380113611214751E-4	0.0	0.4901389362782166	1.3291903356697397E-5
62-63	2.6583806713394795E-5	6.579492161565212E-4	0.0	0.49689786913509715	1.3291903356697397E-5
64-65	2.6583806713394795E-5	7.244087329400081E-4	0.0	0.5010582348857435	1.3291903356697397E-5
66-67	2.6583806713394795E-5	7.576384913317517E-4	0.0	0.5062022014847853	1.3291903356697397E-5
68-69	2.6583806713394795E-5	7.576384913317517E-4	0.0	0.5105353619790687	1.3291903356697397E-5
70-71	2.6583806713394795E-5	7.576384913317517E-4	0.0	0.5139580270934183	1.3291903356697397E-5
72-73	2.6583806713394795E-5	7.576384913317517E-4	0.0	0.517460443627908	1.3291903356697397E-5
74-75	2.6583806713394795E-5	7.576384913317517E-4	0.0	0.5205042894965917	1.3291903356697397E-5
76-77	2.6583806713394795E-5	7.908682497234953E-4	0.0	0.5228037887773004	1.3291903356697397E-5
78-79	2.6583806713394795E-5	7.975142014018439E-4	0.0	0.5242725440982154	1.3291903356697397E-5
80-81	2.6583806713394795E-5	8.307439597935874E-4	0.0	0.5249437852177288	1.3291903356697397E-5
82-83	2.6583806713394795E-5	8.37389911471936E-4	0.0	0.5257014237090605	1.3291903356697397E-5
84-85	2.6583806713394795E-5	8.37389911471936E-4	0.0	0.5262397457950067	1.3291903356697397E-5
86-87	2.6583806713394795E-5	8.37389911471936E-4	0.0	0.5266052731373159	1.3291903356697397E-5
88	2.6583806713394795E-5	8.37389911471936E-4	0.0	0.5266385028957076	1.3291903356697397E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15175	0.0	47.73568	1
GTATCAA	26875	0.0	36.89683	1
ATCAACG	32650	0.0	30.099358	3
ACGCAGA	34085	0.0	29.026577	7
AACGCAG	34455	0.0	28.562592	6
TCAACGC	34500	0.0	28.510181	4
TATCAAC	34765	0.0	28.389063	2
CAACGCA	34980	0.0	28.098188	5
CGCAGAG	38120	0.0	26.609001	8
GCAGAGT	40365	0.0	23.381754	9
TACATGG	26330	0.0	23.054556	2
GAGTACT	25845	0.0	22.813692	12-13
GTACATG	28185	0.0	22.421171	1
ACATGGG	26345	0.0	22.336853	3
AGACCGA	4960	0.0	21.153738	88-89
CAGAGTA	39090	0.0	21.085453	10-11
AGAGTAC	37385	0.0	20.85896	10-11
ACCGAGG	5215	0.0	20.799925	90-91
CATGGGG	18565	0.0	20.283043	4
AGTACTT	27900	0.0	20.159317	12-13
>>END_MODULE