FastQCFastQC Report
Fri 27 May 2016
SRR2139416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7937949
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109900.049.194391
GTATCAA197300.038.860561
ATCAACG245900.030.5737483
TCAACGC250000.030.0717664
TATCAAC253500.029.9515082
CAACGCA252500.029.7217885
AACGCAG262900.028.6125096
ACGCAGA290000.025.8631847
CGCAGAG302000.024.8217838
TACATGG254400.024.7478662
GTACATG264750.024.2768441
TATCTCG34750.023.83691894
ACATGGG264700.023.2382323
CTACGCT96700.022.48577194
TACGCTA68200.022.26701594
GAGTACT176800.022.1774812-13
GCAGAGT332050.021.8401749
CATGGGG201700.020.7868044
AGAGTAC283600.020.43375410-11
CAGAGTA307500.020.42499210-11
Produced by FastQC (version 0.11.5)