##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7937949
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.693888685855754	33.0	33.0	33.0	33.0	33.0
2	32.70761087026384	33.0	33.0	33.0	33.0	33.0
3	32.69789589225126	33.0	33.0	33.0	33.0	33.0
4	36.63009727071817	37.0	37.0	37.0	37.0	37.0
5	36.64705807507708	37.0	37.0	37.0	37.0	37.0
6	36.60653765853119	37.0	37.0	37.0	37.0	37.0
7	36.578218504553256	37.0	37.0	37.0	37.0	37.0
8	36.583370717045426	37.0	37.0	37.0	37.0	37.0
9	36.598276330573555	37.0	37.0	37.0	37.0	37.0
10-11	36.5444262113551	37.0	37.0	37.0	37.0	37.0
12-13	36.50884598779861	37.0	37.0	37.0	37.0	37.0
14-15	35.53254820609203	40.0	37.0	40.0	19.5	40.0
16-17	35.819658264370304	40.0	37.0	40.0	24.0	40.0
18-19	38.09677827358175	40.0	37.0	40.0	35.0	40.0
20-21	38.42193634255619	40.0	37.0	40.0	37.0	40.0
22-23	38.45398219922589	40.0	37.0	40.0	37.0	40.0
24-25	38.48216026656917	40.0	37.0	40.0	37.0	40.0
26-27	38.420098618149126	40.0	37.0	40.0	37.0	40.0
28-29	38.350470035034874	40.0	37.0	40.0	37.0	40.0
30-31	38.23564925180072	40.0	37.0	40.0	37.0	40.0
32-33	38.0492055585097	40.0	37.0	40.0	35.0	40.0
34-35	37.9629625619247	40.0	37.0	40.0	33.0	40.0
36-37	37.904023271411326	40.0	37.0	40.0	33.0	40.0
38-39	37.89895045375068	40.0	37.0	40.0	33.0	40.0
40-41	37.842493702641406	40.0	37.0	40.0	33.0	40.0
42-43	37.79031058963196	40.0	37.0	40.0	33.0	40.0
44-45	37.74242002806284	40.0	37.0	40.0	33.0	40.0
46-47	37.604388080505444	40.0	37.0	40.0	33.0	40.0
48-49	37.36963085456394	40.0	37.0	40.0	33.0	40.0
50-51	37.266507255871986	40.0	37.0	40.0	33.0	40.0
52-53	37.18013318760889	40.0	37.0	40.0	33.0	40.0
54-55	36.954453851285535	37.0	37.0	40.0	33.0	40.0
56-57	36.83185407886157	37.0	37.0	40.0	33.0	40.0
58-59	36.76836251119442	37.0	37.0	40.0	33.0	40.0
60-61	36.63677014469806	37.0	37.0	40.0	33.0	40.0
62-63	36.44087497604559	37.0	37.0	40.0	33.0	40.0
64-65	36.16448811631199	37.0	37.0	40.0	33.0	40.0
66-67	35.82589394593498	37.0	33.0	40.0	33.0	40.0
68-69	35.52313777610934	37.0	33.0	37.0	33.0	40.0
70-71	35.27574405238587	37.0	33.0	37.0	30.0	40.0
72-73	35.06459985100576	37.0	33.0	37.0	27.0	40.0
74-75	34.78002749432737	37.0	33.0	37.0	27.0	38.5
76-77	31.871715010161836	33.0	30.0	35.0	24.5	37.0
78-79	34.04658556027938	37.0	33.0	37.0	27.0	37.0
80-81	34.38645913936466	37.0	33.0	37.0	27.0	37.0
82-83	34.37130133509243	37.0	33.0	37.0	27.0	37.0
84-85	34.34617057834002	37.0	33.0	37.0	27.0	37.0
86-87	34.17962106696201	37.0	33.0	37.0	27.0	37.0
88-89	33.956958513009155	37.0	33.0	37.0	27.0	37.0
90-91	33.803942241414404	37.0	33.0	37.0	27.0	37.0
92-93	33.83680433901266	37.0	33.0	37.0	27.0	37.0
94-95	33.77586440726826	35.0	33.0	37.0	27.0	37.0
96-97	33.71579792697124	33.0	33.0	37.0	27.0	37.0
98-99	33.71357031988327	33.0	33.0	37.0	27.0	37.0
100	33.74281481540441	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	9.0
20	53.0
21	277.0
22	1167.0
23	3472.0
24	8318.0
25	16763.0
26	30407.0
27	49701.0
28	74271.0
29	105261.0
30	144456.0
31	193143.0
32	260081.0
33	356235.0
34	504719.0
35	794988.0
36	1436992.0
37	2505084.0
38	1433235.0
39	19317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.693496771017294	19.42858287449315	13.74189982828058	24.136020526208974
2	15.069863548328009	20.425749776200313	41.10229629945041	23.40209037602127
3	18.332558140706784	25.984036910927095	29.957361046091798	25.72604390227433
4	12.688451469459439	17.562663463812115	38.058929550679466	31.68995551604898
5	13.675980629228821	37.63907734740718	35.00305305949063	13.681888963873368
6	30.752313979341515	37.10802374769603	18.41261514781715	13.727047125145301
7	27.143220496881497	32.37885504177464	22.597550072443145	17.880374388900712
8	25.370042059983	35.613783862808894	20.687258131791978	18.328915945416128
9	26.286097328163738	16.479949669618687	20.649490189468338	36.58446281274924
10-11	24.823087172769693	26.253456654861353	28.310341877983845	20.613114294385113
12-13	25.759588528472534	23.812794715612306	28.307261737257317	22.120355018657843
14-15	23.140060217509447	24.648206427995007	26.129213725843627	26.08251962865192
16-17	22.448531730299603	28.00244118474432	26.676878372486396	22.87214871246968
18-19	22.379842702441145	27.04227502595444	28.60740223954576	21.97048003205866
20-21	23.343773944598325	26.39871729393063	28.34198246975091	21.915526291720138
22-23	23.073795374181923	26.30916785258729	28.135012062275905	22.48202471095488
24-25	22.746016613982135	26.501320070011325	28.280510891325967	22.472152424680573
26-27	22.769856732592032	26.747953622269407	28.22763752707006	22.254552118068503
28-29	22.610130966281677	26.71033807657201	28.301544204432265	22.377986752714047
30-31	22.895098597084974	26.657027334182338	28.056046127849577	22.39182794088311
32-33	22.78056109309671	26.78887663757972	28.036410671640716	22.394151597682857
34-35	22.73300575028168	26.91088371473125	27.999368575654778	22.356741959332293
36-37	22.826592217745322	26.75569886369262	27.857219381531344	22.560489537030715
38-39	22.71815333747031	26.919062043251767	27.98508907928079	22.377695539997127
40-41	22.924283083130696	26.718918724181762	27.995527628562343	22.361270564125192
42-43	22.917035873678035	26.755050719926484	27.724267908080858	22.603645498314627
44-45	22.908328117856573	27.1158357816504	27.403912661806203	22.571923438686824
46-47	23.03182387292619	26.936103863047457	26.988323548905463	23.043748715120888
48-49	22.938825420289355	26.87564809091097	27.139384431823988	23.046142056975686
50-51	22.79403485036118	27.08165362075831	27.09306428392501	23.0312472449555
52-53	22.91262179176476	27.139189296672996	26.882479374168177	23.06570953739407
54-55	23.11380911085038	26.92903293831723	26.925559879699552	23.031598071132837
56-57	22.994321095430802	26.989109732254278	27.010296434924523	23.006272737390393
58-59	22.844940141758503	26.99657948196803	27.189904510950306	22.96857586532316
60-61	23.12515950633931	27.094452399333115	26.995356394471663	22.785031699855914
62-63	22.79325683290354	27.269712016683243	26.964368098094067	22.97266305231915
64-65	22.686532811405932	27.29295945282695	26.84547349089625	23.175034244870872
66-67	22.78753823802449	27.408635419264982	26.70925040551417	23.094575937196364
68-69	22.85982257136076	27.363318417415556	26.756988193750153	23.019870817473535
70-71	22.85724220278008	26.943582969109098	26.62907985963457	23.570094968476255
72-73	22.87011877425737	27.10349994015197	26.461605770725647	23.564775514865012
74-75	22.698301519623104	27.306493127407567	26.704656034372675	23.290549318596657
76-77	23.026706993961323	26.938109640700475	26.47684363681822	23.558339728519986
78-79	23.048673302798615	27.08879574407359	26.213312016948898	23.649218936178897
80-81	23.115700850400493	27.296998048077054	26.206741546533934	23.380559554988523
82-83	23.28294833027381	27.00728703108826	26.38291682593937	23.32684781269856
84-85	23.13377752090304	27.173611392380376	26.161913519647523	23.53069756706906
86-87	23.01574751706458	27.37349575568077	26.36322231768391	23.247534409570736
88-89	23.193721446999145	27.195463814115108	26.48246499711533	23.128349741770418
90-91	23.06846613589887	27.183737902602562	26.51059246568275	23.237203495815827
92-93	23.235034268479104	26.928835191279273	26.526442284139186	23.309688256102447
94-95	22.713141359377783	27.287778751391283	26.76463443845793	23.234445450773002
96-97	22.77782701169798	27.305718760763376	26.758868705076665	23.157585522461982
98-99	23.124232042186016	27.84832862498931	25.88000238058176	23.147436952242913
100	21.169269587071394	27.690250979432335	27.161989303103173	23.978490130393094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1560.0
1	1489.0
2	2406.0
3	3637.0
4	5173.5
5	8967.0
6	11953.0
7	13247.0
8	13490.5
9	12353.5
10	11021.5
11	10033.0
12	11003.5
13	15047.0
14	24103.0
15	36347.0
16	46878.5
17	53386.5
18	53390.0
19	47993.5
20	41022.5
21	34261.5
22	29976.5
23	29655.0
24	32322.0
25	38105.0
26	47738.5
27	60225.0
28	71194.5
29	82339.5
30	93171.5
31	104193.0
32	119749.5
33	135210.5
34	150957.0
35	167779.0
36	189550.5
37	207600.0
38	214431.0
39	223234.0
40	236159.5
41	245953.5
42	252028.5
43	260994.0
44	274326.0
45	287870.5
46	292735.5
47	296533.5
48	311111.5
49	323797.0
50	332267.5
51	337961.0
52	330908.5
53	316589.0
54	301880.0
55	283045.5
56	262446.5
57	243152.5
58	223079.5
59	197068.0
60	160961.0
61	124420.5
62	95322.0
63	72235.0
64	54590.0
65	40726.0
66	30438.5
67	23103.5
68	17333.0
69	12918.0
70	9926.5
71	7600.0
72	5625.5
73	4160.0
74	3121.5
75	2199.5
76	1458.0
77	1031.0
78	714.0
79	487.0
80	353.0
81	261.0
82	193.5
83	140.0
84	96.0
85	69.0
86	53.0
87	43.5
88	27.5
89	10.0
90	4.0
91	3.5
92	3.5
93	3.0
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	5.920924913979669E-4
3	0.0017258866238621588
4	3.77931377488064E-4
5	1.2597712582935466E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	6.97558021599786
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008967088453805505
26-27	0.024945871808100994
28-29	0.00968961195375128
30-31	1.3261063484247182E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.02845490268481791
50-51	0.0743818907328066
52-53	0.06251027569539326
54-55	0.04228908368730097
56-57	0.005603868089680832
58-59	0.008437686531375834
60-61	0.03182488247478234
62-63	0.03313016917446696
64-65	0.0
66-67	0.003096796967378921
68-69	0.03484917275316206
70-71	0.08841354606912924
72-73	0.08102493379435338
74-75	0.04020079248844356
76-77	0.008791595845477911
78-79	0.001109537599158817
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0016926159037299463
92-93	0.0032332685699840986
94-95	0.0012267993082078702
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	203.0
20-21	829.0
22-23	1783.0
24-25	2925.0
26-27	4653.0
28-29	6968.0
30-31	10888.0
32-33	12709.0
34-35	15382.0
36-37	15977.0
38-39	18647.0
40-41	23958.0
42-43	33443.0
44-45	46788.0
46-47	41834.0
48-49	36965.0
50-51	23422.0
52-53	17035.0
54-55	17445.0
56-57	16834.0
58-59	27893.0
60-61	49431.0
62-63	38667.0
64-65	41946.0
66-67	56948.0
68-69	35656.0
70-71	55887.0
72-73	40367.0
74-75	33096.0
76-77	32076.0
78-79	42355.0
80-81	52392.0
82-83	63985.0
84-85	79075.0
86-87	85546.0
88-89	93880.0
90-91	99817.0
92-93	107731.0
94-95	136054.0
96-97	322790.0
98-99	63135.0
100-101	6030534.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.44422862556915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.12234018290106	71.77056020876373
2	7.836957708724758	12.765499507165915
3	2.1032431394601576	5.1389104531605865
4	0.821658235365263	2.6767728469268075
5	0.3949869780485475	1.608470487215428
6	0.22204186359437209	1.0850416981816486
7	0.13487413313429855	0.7689303814265683
8	0.08984049208091947	0.5853591661497631
9	0.06026343528336952	0.4417298100882845
>10	0.20922920441216766	2.638731116936787
>50	0.0030528958633149307	0.16758627144957092
>100	0.0013098746345928133	0.18828941770302451
>500	1.1534657392328652E-4	0.06832013870984227
>1k	8.650992319880127E-5	0.09579849612213756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2597712582935466E-5	1.2597712582935466E-5
5	0.0	0.0	0.0	1.2597712582935466E-5	1.2597712582935466E-5
6	0.0	0.0	0.0	1.2597712582935466E-5	2.5195425165870933E-5
7	0.0	0.0	0.0	1.2597712582935466E-5	3.7793137748806396E-5
8	0.0	0.0	0.0	1.2597712582935466E-5	3.7793137748806396E-5
9	0.0	0.0	0.0	5.0390850331741865E-5	3.7793137748806396E-5
10-11	0.0	0.0	0.0	6.298856291467733E-5	3.7793137748806396E-5
12-13	0.0	0.0	0.0	6.298856291467733E-5	3.7793137748806396E-5
14-15	0.0	0.0	0.0	6.298856291467733E-5	5.66897066232096E-5
16-17	0.0	0.0	0.0	6.298856291467733E-5	6.298856291467733E-5
18-19	0.0	0.0	0.0	6.298856291467733E-5	6.298856291467733E-5
20-21	0.0	0.0	0.0	6.298856291467733E-5	6.928741920614506E-5
22-23	0.0	0.0	0.0	1.0078170066348373E-4	7.558627549761279E-5
24-25	0.0	0.0	0.0	2.1416111390990292E-4	8.818398808054827E-5
26-27	0.0	0.0	0.0	3.77931377488064E-4	8.818398808054827E-5
28-29	0.0	0.0	0.0	0.0013164609649167563	8.818398808054827E-5
30-31	0.0	0.0	0.0	0.0038297046252123816	8.818398808054827E-5
32-33	0.0	0.0	0.0	0.00852865141864731	8.818398808054827E-5
34-35	0.0	0.0	0.0	0.01349844903261535	8.818398808054827E-5
36-37	0.0	0.0	0.0	0.01946976479692676	8.818398808054827E-5
38-39	0.0	0.0	0.0	0.03308159324278853	8.818398808054827E-5
40-41	0.0	0.0	0.0	0.047896503240320645	1.0078170066348373E-4
42-43	0.0	0.0	0.0	0.0612185842967749	1.0078170066348373E-4
44-45	0.0	0.0	0.0	0.08490858280898504	1.0078170066348373E-4
46-47	0.0	0.0	0.0	0.11249127450932225	1.1967826953788693E-4
48-49	0.0	0.0	0.0	0.13587892791954193	1.2597712582935466E-4
50-51	0.0	0.0	0.0	0.1599783520906975	1.2597712582935466E-4
52-53	0.0	0.0	0.0	0.34337585187307196	1.4487369470375785E-4
54-55	0.0	0.0	0.0	0.5283921577223537	1.7006911986962878E-4
56-57	0.0	0.0	0.0	0.7101393571563637	1.7636797616109654E-4
58-59	0.0	0.0	0.0	1.0735140777548458	1.7636797616109654E-4
60-61	0.0	0.0	0.0	1.2113708465499085	1.7636797616109654E-4
62-63	0.0	0.0	0.0	1.2167059778287816	1.88965688744032E-4
64-65	0.0	0.0	0.0	1.2198176128367666	1.88965688744032E-4
66-67	0.0	0.0	0.0	1.222715086730842	1.9526454503549973E-4
68-69	0.0	0.0	0.0	1.2253228132355096	2.0156340132696746E-4
70-71	0.0	0.0	0.0	1.2273195506799048	2.267588264928384E-4
72-73	0.0	0.0	0.0	1.2294296675375467	2.519542516587093E-4
74-75	0.0	0.0	0.0	1.2309350941912074	2.519542516587093E-4
76-77	0.0	0.0	0.0	1.2321948654495007	2.519542516587093E-4
78-79	0.0	0.0	0.0	1.2334609355640858	2.519542516587093E-4
80-81	0.0	0.0	0.0	1.2338451657978653	2.5825310795017707E-4
82-83	0.0	0.0	0.0	1.2343175800197255	2.7714967682458024E-4
84-85	0.0	0.0	0.0	1.2347773965290028	2.83448533116048E-4
86-87	0.0	0.0	0.0	1.2351112359124503	2.897473894075157E-4
88	0.0	0.0	0.0	1.2351427301939077	3.1494281457338663E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10990	0.0	49.19439	1
GTATCAA	19730	0.0	38.86056	1
ATCAACG	24590	0.0	30.573748	3
TCAACGC	25000	0.0	30.071766	4
TATCAAC	25350	0.0	29.951508	2
CAACGCA	25250	0.0	29.721788	5
AACGCAG	26290	0.0	28.612509	6
ACGCAGA	29000	0.0	25.863184	7
CGCAGAG	30200	0.0	24.821783	8
TACATGG	25440	0.0	24.747866	2
GTACATG	26475	0.0	24.276844	1
TATCTCG	3475	0.0	23.836918	94
ACATGGG	26470	0.0	23.238232	3
CTACGCT	9670	0.0	22.485771	94
TACGCTA	6820	0.0	22.267015	94
GAGTACT	17680	0.0	22.17748	12-13
GCAGAGT	33205	0.0	21.840174	9
CATGGGG	20170	0.0	20.786804	4
AGAGTAC	28360	0.0	20.433754	10-11
CAGAGTA	30750	0.0	20.424992	10-11
>>END_MODULE