FastQCFastQC Report
Fri 27 May 2016
SRR2139415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6234694
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103650.053.3336831
GTATCAA190000.038.4972761
TCAACGC236350.030.507954
ATCAACG238950.030.1400343
TATCAAC243400.029.7847162
CAACGCA241950.029.7839575
AACGCAG244900.029.442856
CGCAGAG269700.026.7042458
ACGCAGA278400.025.8533677
GTGGTAT45350.025.2760141
TGGTATC44100.023.8345932
GTACATG196000.023.7904511
TACATGG201400.023.0881422
GCAGAGT299250.023.0784119
GAGTACT189750.022.3225812-13
ACATGGG206100.021.8478783
CAGAGTA283350.021.53076710-11
AGAGTAC264550.021.19752710-11
GTACTTT209750.020.18273414-15
AGTACTT203900.019.46512-13
Produced by FastQC (version 0.11.5)