##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139415_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6234694
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7715947246168	33.0	33.0	33.0	33.0	33.0
2	32.77846338569303	33.0	33.0	33.0	33.0	33.0
3	32.763766273052056	33.0	33.0	33.0	33.0	33.0
4	36.70710848038412	37.0	37.0	37.0	37.0	37.0
5	36.72649852582982	37.0	37.0	37.0	37.0	37.0
6	36.692558608329456	37.0	37.0	37.0	37.0	37.0
7	36.67192423557596	37.0	37.0	37.0	37.0	37.0
8	36.677534935956764	37.0	37.0	37.0	37.0	37.0
9	36.686310346586374	37.0	37.0	37.0	37.0	37.0
10-11	36.635638974422804	37.0	37.0	37.0	37.0	37.0
12-13	36.606922168112824	37.0	37.0	37.0	37.0	37.0
14-15	35.450771280835916	40.0	37.0	40.0	19.5	40.0
16-17	35.759926790312406	40.0	37.0	40.0	24.0	40.0
18-19	38.244057847907214	40.0	37.0	40.0	37.0	40.0
20-21	38.609261635599836	40.0	37.0	40.0	37.0	40.0
22-23	38.652567896073684	40.0	37.0	40.0	37.0	40.0
24-25	38.6857343352232	40.0	37.0	40.0	37.0	40.0
26-27	38.63555288464636	40.0	37.0	40.0	37.0	40.0
28-29	38.569762293614886	40.0	37.0	40.0	37.0	40.0
30-31	38.446151192927886	40.0	37.0	40.0	37.0	40.0
32-33	38.26773614452942	40.0	37.0	40.0	37.0	40.0
34-35	38.188228264030094	40.0	37.0	40.0	37.0	40.0
36-37	38.11623811623562	40.0	37.0	40.0	37.0	40.0
38-39	38.11833351165766	40.0	37.0	40.0	37.0	40.0
40-41	38.06198281336762	40.0	37.0	40.0	37.0	40.0
42-43	38.013934887471294	40.0	37.0	40.0	33.0	40.0
44-45	37.96194085602018	40.0	37.0	40.0	33.0	40.0
46-47	37.822508700745196	40.0	37.0	40.0	33.0	40.0
48-49	37.608412901416386	40.0	37.0	40.0	33.0	40.0
50-51	37.519403489757366	40.0	37.0	40.0	33.0	40.0
52-53	37.444785163030474	40.0	37.0	40.0	33.0	40.0
54-55	37.23633090884489	38.5	37.0	40.0	33.0	40.0
56-57	37.13153634295922	37.0	37.0	40.0	33.0	40.0
58-59	37.07231918670564	37.0	37.0	40.0	33.0	40.0
60-61	36.944207666557006	37.0	37.0	40.0	33.0	40.0
62-63	36.757517121371414	37.0	37.0	40.0	33.0	40.0
64-65	36.49145166509458	37.0	37.0	40.0	33.0	40.0
66-67	36.16611774782248	37.0	37.0	40.0	33.0	40.0
68-69	35.87390641563657	37.0	35.0	38.5	33.0	40.0
70-71	35.63506044476115	37.0	33.0	37.0	33.0	40.0
72-73	35.42011414192875	37.0	33.0	37.0	33.0	40.0
74-75	35.13534186938311	37.0	33.0	37.0	33.0	40.0
76-77	32.27296329714134	33.0	30.0	35.0	27.0	37.0
78-79	34.40326795659521	37.0	33.0	37.0	27.0	37.0
80-81	34.72340573122032	37.0	33.0	37.0	33.0	37.0
82-83	34.701039912319786	37.0	33.0	37.0	33.0	37.0
84-85	34.6616012273452	37.0	33.0	37.0	33.0	37.0
86-87	34.496320727871854	37.0	33.0	37.0	33.0	37.0
88-89	34.2759022392717	37.0	33.0	37.0	27.0	37.0
90-91	34.12255806744233	37.0	33.0	37.0	27.0	37.0
92-93	34.13333143919544	37.0	33.0	37.0	27.0	37.0
94-95	34.06244245603732	37.0	33.0	37.0	27.0	37.0
96-97	33.99142527191227	37.0	33.0	37.0	27.0	37.0
98-99	33.97550940383835	33.0	33.0	37.0	27.0	37.0
100	34.02094368236464	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	2.0
19	13.0
20	40.0
21	157.0
22	610.0
23	1823.0
24	4320.0
25	9199.0
26	16751.0
27	27878.0
28	43175.0
29	63150.0
30	89182.0
31	124231.0
32	171901.0
33	245063.0
34	360985.0
35	590362.0
36	1104875.0
37	2057976.0
38	1301046.0
39	21954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.960422218604464	19.663270878961807	14.106229905761078	23.270076996672646
2	14.903536856294192	20.99215979826329	41.501935710698625	22.602367634743896
3	18.49924966974562	26.199293361485953	30.17918746194616	25.122269506822263
4	12.785476307639797	17.97250471075605	38.25398561719726	30.988033364406892
5	13.636324267172398	37.705683975103156	34.925014667939976	13.732977089784473
6	30.140404645360302	37.60819697005178	18.520957083058125	13.730441301529794
7	26.714238100538694	33.085104096528234	22.56840512140612	17.632252681526953
8	24.7891716899017	36.48406802322616	20.744658839712102	17.982101447160037
9	26.119004397008094	16.858982974946322	21.01650858887381	36.00550403917177
10-11	24.644224720571692	26.778331061636706	28.16827898851171	20.409165229279896
12-13	25.64615841611473	24.52197333181067	28.456953941925615	21.37491431014898
14-15	22.840919798205892	25.401642621867143	26.472192632082713	25.285244947844255
16-17	21.953451123663807	28.746454918236562	27.06764758623278	22.232446371866846
18-19	21.922134430334513	27.558313206710704	29.328167509103093	21.191384853851687
20-21	22.739005239297168	26.712251203960463	29.39473638623231	21.15400717051006
22-23	22.447984494407905	26.723733007872468	29.252503600635393	21.57577889708423
24-25	22.193354687890803	26.894441302075407	29.307721879985287	21.6044821300485
26-27	22.11590783777788	27.042215364593808	29.253186280000488	21.58869051762783
28-29	22.05525645336911	27.10259982451791	29.242374313483765	21.599769408629214
30-31	22.307761648157413	26.964515737604128	29.1552544333315	21.57246818090696
32-33	22.16204430686833	27.16547064368739	29.04290024994378	21.6295847995005
34-35	22.265045785688745	27.160055877124556	29.06441645387809	21.51048188330861
36-37	22.273555370389847	27.141951089286724	28.878564552272014	21.70592898805142
38-39	22.133018414278386	27.29888841264846	28.948935748675513	21.619157424397642
40-41	22.44272385971695	27.120811501012344	28.75768311170348	21.678781527567224
42-43	22.35339225960946	27.284752441977794	28.472776315389485	21.889078983023257
44-45	22.384569121227837	27.43758213741045	28.24533305128922	21.932515690072496
46-47	22.625529805364707	27.479462671805965	27.73493238788473	22.1600751349446
48-49	22.490770638211842	27.71177446119349	27.607814876852416	22.189640023742253
50-51	22.49294571142296	27.855072531466114	27.468041745506426	22.1839400116045
52-53	22.69609706560202	27.74127961024818	27.330150976258892	22.2324723478909
54-55	22.617914465657964	27.75155594230978	27.32192609987012	22.30860349216214
56-57	22.672844495213926	27.87201534783188	27.229476746246146	22.225663410708048
58-59	22.593593710990067	27.82950201877857	27.272010575823302	22.30489369440806
60-61	22.66367948524359	27.83962332595938	27.323771378929123	22.172925809867902
62-63	22.66044795331674	28.010804084785036	27.18167853771561	22.147069424182614
64-65	22.614932419279484	27.94349009608726	27.13664069512436	22.304936789508897
66-67	22.699640713832775	27.910590733828883	27.041010159693084	22.34875839264526
68-69	22.63455629121884	27.942354037556825	27.112295179427683	22.310794491796653
70-71	22.685884255214596	27.7004693478844	27.12043296138143	22.493213435519575
72-73	22.730581424495046	27.74079796380286	26.99899282592302	22.52962778577907
74-75	22.60820699392028	27.86902141446324	26.985058133172597	22.537713458443886
76-77	22.76498891518894	27.766691110998963	26.94579127007821	22.52252870373388
78-79	22.663789324672617	27.77334113407876	26.968780312777934	22.594089228470686
80-81	22.732222937238696	27.863396351818707	26.898114968857158	22.50626574208544
82-83	22.79109856275436	27.787868823198085	26.99385772662698	22.427174887420573
84-85	22.816866607879852	27.750165127655734	26.933086947046636	22.49988131741778
86-87	22.699576965421457	27.8316326361232	27.001195241759724	22.467595156695616
88-89	22.774544626102294	27.749503494810597	26.95457478647964	22.52137709260747
90-91	22.83210117681719	27.774921673996317	26.963975628466514	22.429001520719982
92-93	22.834671304500667	27.67516586697985	27.038042921935286	22.452119906584198
94-95	22.76563174148462	27.76927777566763	27.01413475958221	22.450955723265547
96-97	22.83250457937923	27.84360687972075	27.053758556359636	22.27012998454039
98-99	23.01072419238387	28.671558871543652	26.02527704783183	22.292439888240647
100	21.114212526211503	27.979443096146582	27.26843506730232	23.63790931033959
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1343.0
1	1250.5
2	1914.0
3	2897.0
4	4146.5
5	7127.5
6	9543.5
7	10912.5
8	11857.0
9	11735.5
10	11070.5
11	10441.0
12	11442.5
13	15403.0
14	24559.5
15	36975.5
16	47721.0
17	54587.5
18	55061.5
19	49850.5
20	42627.5
21	35131.5
22	29389.5
23	27577.5
24	29621.0
25	35374.5
26	43680.0
27	52803.0
28	63618.0
29	76216.5
30	88395.0
31	100502.0
32	114478.0
33	128408.5
34	141272.5
35	153003.0
36	165806.5
37	178750.0
38	188836.0
39	197158.5
40	205205.0
41	213385.5
42	218442.0
43	222430.0
44	227206.0
45	232256.5
46	237034.5
47	239226.0
48	240598.0
49	240211.5
50	236902.5
51	233197.5
52	226900.0
53	216624.0
54	205577.0
55	193967.5
56	179784.0
57	162661.5
58	145362.0
59	127878.5
60	107941.5
61	88799.5
62	71824.0
63	56043.0
64	42306.5
65	31539.0
66	24059.5
67	18799.0
68	14406.0
69	10439.0
70	7541.5
71	5629.5
72	4085.0
73	2845.0
74	1950.0
75	1385.5
76	992.5
77	667.0
78	452.0
79	327.0
80	234.5
81	175.0
82	134.5
83	102.5
84	76.0
85	51.5
86	34.5
87	27.5
88	21.0
89	10.5
90	4.5
91	2.5
92	1.5
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.4435351598651033E-4
2	5.292962252838712E-4
3	0.0017001636327300105
4	4.170212684054743E-4
5	1.2831423643245362E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.48292538495073
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008529005680012889
26-27	0.02317545658940765
28-29	0.008570927558311426
30-31	1.1257761321789839E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.02666388762138774
50-51	0.06760270150731518
52-53	0.05728508680192428
54-55	0.03898783122862303
56-57	0.005466606961420265
58-59	0.011005370739714497
60-61	0.039439230015022274
62-63	0.04055678773771356
64-65	0.0
66-67	0.002818601832560958
68-69	0.03161822089609386
70-71	0.08389161732882909
72-73	0.075541095148212
74-75	0.03689704379809113
76-77	0.008286853682960324
78-79	0.0011711875869368459
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0015691434222586526
92-93	0.0032555655941042707
94-95	0.0011812176215266728
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	151.0
20-21	622.0
22-23	1324.0
24-25	3108.0
26-27	3672.0
28-29	5716.0
30-31	9645.0
32-33	11011.0
34-35	14956.0
36-37	16912.0
38-39	19257.0
40-41	23248.0
42-43	27889.0
44-45	35351.0
46-47	34195.0
48-49	24088.0
50-51	22149.0
52-53	20352.0
54-55	25792.0
56-57	30780.0
58-59	44743.0
60-61	23533.0
62-63	29596.0
64-65	39896.0
66-67	52971.0
68-69	39231.0
70-71	47578.0
72-73	41395.0
74-75	39044.0
76-77	27926.0
78-79	37982.0
80-81	48906.0
82-83	62736.0
84-85	77530.0
86-87	81511.0
88-89	88108.0
90-91	91982.0
92-93	94545.0
94-95	105512.0
96-97	243759.0
98-99	40755.0
100-101	4545237.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.21612256529717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.1218748552211	81.26634603173152
2	5.697227833487472	10.051746976827065
3	1.225586910609401	3.2434957536222853
4	0.43718623035635507	1.5426749632390586
5	0.18566956514133504	0.818952455757672
6	0.10402742753714621	0.5506137778661675
7	0.06294329425336041	0.3886829352362605
8	0.03829047603917994	0.2702269861884698
9	0.026045444197448357	0.20678652878307413
>10	0.09789745838576437	1.3707421825069315
>50	0.002472860824677757	0.14455264611966331
>100	7.497054789855984E-4	0.12292321376390598
>500	2.793846775154854E-5	0.02225554835797859
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6039279554056702E-5	0.0
2	0.0	4.8117838662170106E-5	0.0	1.6039279554056702E-5	0.0
3	0.0	4.8117838662170106E-5	0.0	1.6039279554056702E-5	0.0
4	0.0	9.623567732434021E-5	0.0	1.6039279554056702E-5	0.0
5	0.0	1.1227495687839691E-4	0.0	1.6039279554056702E-5	0.0
6	0.0	1.1227495687839691E-4	0.0	3.2078559108113404E-5	0.0
7	0.0	1.1227495687839691E-4	0.0	4.8117838662170106E-5	0.0
8	0.0	1.1227495687839691E-4	0.0	4.8117838662170106E-5	0.0
9	0.0	2.0851063420273714E-4	0.0	6.415711821622681E-5	0.0
10-11	0.0	2.0851063420273714E-4	0.0	9.623567732434021E-5	0.0
12-13	8.019639777028351E-6	2.0851063420273714E-4	0.0	1.1227495687839691E-4	8.019639777028351E-6
14-15	1.6039279554056702E-5	2.0851063420273714E-4	0.0	1.1227495687839691E-4	5.613747843919846E-5
16-17	1.6039279554056702E-5	2.0851063420273714E-4	0.0	1.2831423643245362E-4	8.019639777028351E-5
18-19	1.6039279554056702E-5	2.325695535338222E-4	0.0	1.6039279554056705E-4	8.019639777028351E-5
20-21	1.6039279554056702E-5	2.5662847286490723E-4	0.0	2.5662847286490723E-4	8.019639777028351E-5
22-23	1.6039279554056702E-5	2.806873921959923E-4	0.0	4.0900162862844594E-4	8.019639777028351E-5
24-25	1.6039279554056702E-5	3.0474631152707735E-4	0.0	9.543371334663738E-4	8.019639777028351E-5
26-27	1.6039279554056702E-5	3.0474631152707735E-4	0.0	0.0020851063420273715	8.019639777028351E-5
28-29	1.6039279554056702E-5	3.0474631152707735E-4	0.0	0.007105400842447119	8.019639777028351E-5
30-31	1.6039279554056702E-5	3.6890342974330416E-4	0.0	0.010305237113481431	8.019639777028351E-5
32-33	2.4058919331085053E-5	3.6890342974330416E-4	0.0	0.01794795382098945	1.1227495687839691E-4
34-35	3.2078559108113404E-5	4.4909982751358766E-4	0.0	0.028429623009565506	1.1227495687839691E-4
36-37	3.2078559108113404E-5	5.132569457298145E-4	0.0	0.042239442705608324	1.1227495687839691E-4
38-39	3.2078559108113404E-5	5.292962252838712E-4	0.0	0.06576906581140951	1.1227495687839691E-4
40-41	3.2078559108113404E-5	6.415711821622681E-4	0.0	0.0833320769231016	1.1227495687839691E-4
42-43	3.2078559108113404E-5	6.977086606014665E-4	0.0	0.09325237132728567	1.2831423643245362E-4
44-45	3.2078559108113404E-5	8.581014561420337E-4	0.0	0.10134418786230728	1.4435351598651033E-4
46-47	4.8117838662170106E-5	9.623567732434022E-4	0.0	0.10969263287019379	1.5237315576353868E-4
48-49	4.8117838662170106E-5	9.703764130204305E-4	0.0	0.11595597153605293	1.6039279554056702E-4
50-51	4.8117838662170106E-5	0.001074631730121799	0.0	0.1216017979390809	1.6039279554056702E-4
52-53	4.8117838662170106E-5	0.0010906710096758557	0.0	0.143415218132598	1.6039279554056702E-4
54-55	4.8117838662170106E-5	0.0010906710096758557	0.0	0.16728968574881142	1.6039279554056702E-4
56-57	4.8117838662170106E-5	0.0010906710096758557	0.0	0.19493338406022814	1.684124353175954E-4
58-59	4.8117838662170106E-5	0.0011067102892299125	0.0	0.21894418555265102	1.8445171487165208E-4
60-61	4.8117838662170106E-5	0.0011147299290069409	0.0	0.23673976621787693	1.9247135464868043E-4
62-63	4.8117838662170106E-5	0.0011227495687839693	0.0	0.2453608789781824	1.9247135464868043E-4
64-65	4.8117838662170106E-5	0.001299181643878593	0.0	0.24760637811575034	1.9247135464868043E-4
66-67	4.8117838662170106E-5	0.001299181643878593	0.0	0.25083829294589277	1.9247135464868043E-4
68-69	4.8117838662170106E-5	0.001323240563209678	0.0	0.2550325645492786	2.0049099442570877E-4
70-71	4.8117838662170106E-5	0.0013553191223177913	0.0	0.25746251540171816	2.0851063420273714E-4
72-73	4.8117838662170106E-5	0.0013713584018718481	0.0	0.25970801453928616	2.0851063420273714E-4
74-75	4.8117838662170106E-5	0.00141145660075699	0.0	0.2614001585322391	2.0851063420273714E-4
76-77	4.8117838662170106E-5	0.0014996726383043016	0.0	0.26281963477277315	2.0851063420273714E-4
78-79	5.613747843919846E-5	0.0015477904769664718	0.0	0.2638621879437868	2.0851063420273714E-4
80-81	6.415711821622681E-5	0.0015558101167435002	0.0	0.264207032454199	2.1653027397976549E-4
82-83	6.415711821622681E-5	0.0015558101167435002	0.0	0.26483256435680724	2.325695535338222E-4
84-85	6.415711821622681E-5	0.0015638297565205286	0.0	0.2656425479742871	2.4058919331085055E-4
86-87	6.415711821622681E-5	0.0015718493962975568	0.0	0.2661237263609088	2.4058919331085055E-4
88	6.415711821622681E-5	0.0016039279554056704	0.0	0.26617184419957096	2.4058919331085055E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10365	0.0	53.333683	1
GTATCAA	19000	0.0	38.497276	1
TCAACGC	23635	0.0	30.50795	4
ATCAACG	23895	0.0	30.140034	3
TATCAAC	24340	0.0	29.784716	2
CAACGCA	24195	0.0	29.783957	5
AACGCAG	24490	0.0	29.44285	6
CGCAGAG	26970	0.0	26.704245	8
ACGCAGA	27840	0.0	25.853367	7
GTGGTAT	4535	0.0	25.276014	1
TGGTATC	4410	0.0	23.834593	2
GTACATG	19600	0.0	23.790451	1
TACATGG	20140	0.0	23.088142	2
GCAGAGT	29925	0.0	23.078411	9
GAGTACT	18975	0.0	22.32258	12-13
ACATGGG	20610	0.0	21.847878	3
CAGAGTA	28335	0.0	21.530767	10-11
AGAGTAC	26455	0.0	21.197527	10-11
GTACTTT	20975	0.0	20.182734	14-15
AGTACTT	20390	0.0	19.465	12-13
>>END_MODULE