FastQCFastQC Report
Fri 27 May 2016
SRR2139414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6816184
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209350.054.5492171
GTATCAA395050.039.1018871
TATCAAC507700.030.3344752
ATCAACG506150.030.2607333
TCAACGC515250.029.7831444
CAACGCA519800.029.4494275
AACGCAG521650.029.4177426
GTGGTAT79100.029.1091161
TGGTATC76450.028.9597422
CGCAGAG571600.026.7969638
ACGCAGA585400.026.1277087
GCAGAGT593450.024.8662729
GAGTACT352100.024.44112612-13
TACATGG420100.023.9313072
GTACATG432250.023.5025311
CAGAGTA570850.022.6301810-11
ACATGGG438050.022.4982073
GTACTTT389900.022.08396714-15
AGAGTAC551250.021.52139910-11
AGTACTT370050.021.29095812-13
Produced by FastQC (version 0.11.5)