##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6816184
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.769787171238335	33.0	33.0	33.0	33.0	33.0
2	32.77238539922045	33.0	33.0	33.0	33.0	33.0
3	32.7452279750664	33.0	33.0	33.0	33.0	33.0
4	36.6972930895058	37.0	37.0	37.0	37.0	37.0
5	36.72341899807869	37.0	37.0	37.0	37.0	37.0
6	36.695717721235226	37.0	37.0	37.0	37.0	37.0
7	36.674414745846065	37.0	37.0	37.0	37.0	37.0
8	36.684005596093066	37.0	37.0	37.0	37.0	37.0
9	36.68433261191306	37.0	37.0	37.0	37.0	37.0
10-11	36.640826230629926	37.0	37.0	37.0	37.0	37.0
12-13	36.60926128167901	37.0	37.0	37.0	37.0	37.0
14-15	35.49616427608175	40.0	37.0	40.0	19.5	40.0
16-17	35.79830745472834	40.0	37.0	40.0	24.0	40.0
18-19	38.26346633248164	40.0	37.0	40.0	37.0	40.0
20-21	38.639894560858515	40.0	37.0	40.0	37.0	40.0
22-23	38.68152946727311	40.0	37.0	40.0	37.0	40.0
24-25	38.708800855097195	40.0	37.0	40.0	37.0	40.0
26-27	38.65687037942607	40.0	37.0	40.0	37.0	40.0
28-29	38.58425848630596	40.0	37.0	40.0	37.0	40.0
30-31	38.42489667373597	40.0	37.0	40.0	37.0	40.0
32-33	38.230134466179194	40.0	37.0	40.0	37.0	40.0
34-35	38.148869766248936	40.0	37.0	40.0	37.0	40.0
36-37	38.08661484259647	40.0	37.0	40.0	37.0	40.0
38-39	38.09468688843453	40.0	37.0	40.0	37.0	40.0
40-41	38.05053298120863	40.0	37.0	40.0	37.0	40.0
42-43	38.00272113869784	40.0	37.0	40.0	33.0	40.0
44-45	37.944627952091665	40.0	37.0	40.0	33.0	40.0
46-47	37.804974731060376	40.0	37.0	40.0	33.0	40.0
48-49	37.58740320820897	40.0	37.0	40.0	33.0	40.0
50-51	37.518857177922754	40.0	37.0	40.0	33.0	40.0
52-53	37.46411512275171	40.0	37.0	40.0	33.0	40.0
54-55	37.27131824357285	38.5	37.0	40.0	33.0	40.0
56-57	37.184662972303954	37.0	37.0	40.0	33.0	40.0
58-59	37.14024275441524	37.0	37.0	40.0	33.0	40.0
60-61	37.043609761270375	37.0	37.0	40.0	33.0	40.0
62-63	36.87803678877761	37.0	37.0	40.0	33.0	40.0
64-65	36.6274490968862	37.0	37.0	40.0	33.0	40.0
66-67	36.31668901589731	37.0	37.0	40.0	33.0	40.0
68-69	36.03744187948115	37.0	37.0	40.0	33.0	40.0
70-71	35.81846706298903	37.0	35.0	37.0	33.0	40.0
72-73	35.615092905523774	37.0	33.0	37.0	33.0	40.0
74-75	35.33541731709527	37.0	33.0	37.0	33.0	40.0
76-77	32.44908287480432	33.0	30.0	35.0	27.0	37.0
78-79	34.56526955201467	37.0	33.0	37.0	30.0	37.0
80-81	34.8871265686393	37.0	33.0	37.0	33.0	37.0
82-83	34.85979583914646	37.0	33.0	37.0	33.0	37.0
84-85	34.817018695575015	37.0	33.0	37.0	33.0	37.0
86-87	34.65305513502794	37.0	33.0	37.0	33.0	37.0
88-89	34.43517465070627	37.0	33.0	37.0	33.0	37.0
90-91	34.28243032079526	37.0	33.0	37.0	27.0	37.0
92-93	34.2959168504109	37.0	33.0	37.0	33.0	37.0
94-95	34.22600867178083	37.0	33.0	37.0	30.0	37.0
96-97	34.14708395404554	37.0	33.0	37.0	27.0	37.0
98-99	34.10451567598177	37.0	33.0	37.0	27.0	37.0
100	34.11546827179746	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	7.0
19	14.0
20	26.0
21	166.0
22	596.0
23	1771.0
24	4140.0
25	8975.0
26	16581.0
27	27726.0
28	43461.0
29	64266.0
30	91090.0
31	126527.0
32	177651.0
33	255018.0
34	377183.0
35	626955.0
36	1162722.0
37	2288731.0
38	1512864.0
39	29713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.935068000728265	20.06093738469526	14.41305001322589	21.590944601350582
2	14.824001059835986	21.411210347661118	42.37143290148146	21.393355691021426
3	18.11229654668533	28.279387211967034	30.656933771122908	22.951382470224736
4	11.900655413595409	18.620603349044053	39.44400317246353	30.03473806489701
5	12.419307773257648	39.198131151817215	34.693010466130794	13.689550608794343
6	27.934178420066125	38.89294948610542	19.11328978208335	14.059582311745105
7	25.61769165855851	34.7528910604526	22.69153825659636	16.937879024392537
8	25.182418784469434	35.745910028250414	20.822750676918346	18.24892051036181
9	27.217736493028944	16.28463961653617	21.1601095275597	35.33751436287518
10-11	24.43601874597282	27.623396023346785	27.710871654873166	20.229713575807224
12-13	25.9745555577725	25.625489276697927	28.28057458542786	20.119380580101712
14-15	23.0935367946661	25.912835531124838	27.696476090153077	23.29715158405598
16-17	20.65773165747873	29.29968586528767	28.196099166337056	21.846483310896538
18-19	20.443432865075238	27.48980954739485	31.69911492999602	20.367642657533892
20-21	22.368528000107982	26.459989806245304	31.514620584870347	19.65686160877637
22-23	21.798182724199844	26.463814717926653	31.415233903386568	20.322768654486932
24-25	21.047103696473883	26.74612262847285	31.92758868725708	20.27918498779619
26-27	20.891260947553526	27.138219807473156	31.692876465787297	20.277642779186024
28-29	20.6669401539685	27.389106667175138	31.669873639608248	20.27407953924811
30-31	21.157623912844752	27.108249109453826	31.52044741711065	20.213679560590776
32-33	20.968363922754605	27.361310272361074	31.291768662361413	20.37855714252291
34-35	20.950820009461037	27.497831445086206	31.296057504621157	20.2552910408316
36-37	21.01423577124573	27.34464603334496	31.20010615309242	20.44101204231689
38-39	20.772261684393474	27.66058184377153	31.16198413641083	20.40517233542417
40-41	21.21359137178289	27.57749810778474	30.88959156970662	20.319318950725748
42-43	21.25334156397703	27.74020302723316	30.34773816546065	20.658717243329157
44-45	21.33460270733531	28.300671570545333	29.65299298249244	20.71173273962692
46-47	21.57582121302582	28.279193766463724	28.77989789366729	21.365087126843175
48-49	21.748292741186585	28.37420721429837	28.49752749066331	21.379972553851733
50-51	21.548948549537286	28.703678539577254	28.229274855187125	21.51809805569834
52-53	21.74556592863803	28.743285033728228	27.96283585858546	21.548313179048282
54-55	21.88953228792161	28.725514193234186	27.85575389147874	21.52919962736546
56-57	22.086021508798417	28.854248484161833	27.586209914466377	21.473520092573377
58-59	21.818836597602324	28.732962619075707	27.962513273382957	21.485687509939005
60-61	21.843478168045706	28.872222989581775	27.71152248052587	21.572776361846653
62-63	21.818837565013045	28.916187594652104	27.65035451830447	21.61462032203038
64-65	21.77682959444307	28.961600210683176	27.650318273902847	21.611251920970908
66-67	22.021186518476053	28.814722088011568	27.433808877377192	21.73028251613519
68-69	22.005050249244476	28.768952434348183	27.623494323818733	21.602502992588608
70-71	21.986228421653546	28.414279644212314	27.673156428295613	21.926335505838527
72-73	22.065708522817594	28.463876498154185	27.71627700957487	21.754137969453346
74-75	21.899206095886246	28.600389685396554	27.584672053729996	21.915732164987205
76-77	21.964849563532155	28.579802212304305	27.471721293007285	21.983626931156255
78-79	22.03214774412641	28.57106588259966	27.471782125964168	21.92500424730976
80-81	21.92244296761297	28.41680548492872	27.55101790317207	22.10973364428624
82-83	22.23967110850334	28.31235962811103	27.699600320751177	21.748368942634457
84-85	22.472949909761926	28.31168975814687	27.403749121893213	21.81161121019799
86-87	21.848806903064162	28.501241408444937	27.577055395248077	22.07289629324282
88-89	22.244432394145715	28.27972300702819	27.579948936160704	21.895895662665392
90-91	22.34023308341903	28.206979947886644	27.598895720412507	21.853891248281816
92-93	22.275613171448615	28.267614017862318	27.506861809458012	21.94991100123105
94-95	22.031595342076503	28.327052365119176	27.622953890508526	22.01839840229579
96-97	22.056824776547113	28.45931927294606	27.765834416127444	21.718021534379382
98-99	23.145699804291446	30.412633508657898	27.788268738688277	18.653397948362382
100	22.322089109590223	31.011317348304264	21.61652266573565	25.050070876369862
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2218.0
1	2207.0
2	3800.0
3	5864.5
4	9041.5
5	16191.0
6	21325.5
7	23471.5
8	24095.0
9	22378.0
10	20026.5
11	18373.0
12	21262.0
13	31282.0
14	54116.0
15	84765.5
16	110538.0
17	125680.5
18	123474.5
19	106713.0
20	85640.0
21	64608.5
22	48976.5
23	42511.0
24	42942.5
25	49298.5
26	61679.5
27	76348.0
28	87027.5
29	98197.0
30	109153.0
31	118983.5
32	135122.5
33	152802.0
34	170338.5
35	186843.0
36	206674.5
37	223870.0
38	226269.5
39	226289.0
40	232054.5
41	235094.5
42	234948.0
43	238961.5
44	245881.0
45	252424.0
46	256201.5
47	259228.0
48	258997.0
49	253649.0
50	247090.0
51	240227.0
52	230620.5
53	217578.5
54	203448.0
55	185684.5
56	164958.0
57	143162.0
58	124172.5
59	110577.0
60	94116.5
61	73796.0
62	55451.5
63	41015.0
64	29484.0
65	20646.0
66	14677.5
67	10746.0
68	8004.0
69	5809.5
70	4377.0
71	3039.5
72	2212.0
73	1648.0
74	1129.5
75	805.5
76	571.5
77	406.5
78	272.0
79	184.0
80	135.5
81	95.5
82	68.5
83	49.0
84	32.5
85	23.0
86	19.0
87	15.5
88	12.5
89	9.5
90	6.0
91	2.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.0269675818610531E-4
2	5.134837909305265E-4
3	0.0018191997164395796
4	4.4012896365473703E-4
5	1.0269675818610531E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.264761338602361
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0089895813320813
26-27	0.02430118313724016
28-29	0.008897465199596373
30-31	9.568237690333407E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.027253660451883727
50-51	0.06988361392437856
52-53	0.059000003857238414
54-55	0.04097363744455542
56-57	0.005640712995669699
58-59	0.010466922521210745
60-61	0.039190059176342655
62-63	0.040431961258135356
64-65	0.0
66-67	0.00339076012902752
68-69	0.03410579648147158
70-71	0.08717191914036178
72-73	0.07864172804081632
74-75	0.0390409039974125
76-77	0.009591019553918135
78-79	0.0013824215277983666
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0018451240883993244
92-93	0.003992907973672067
94-95	0.0013216640008140656
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	121.0
20-21	665.0
22-23	1505.0
24-25	2533.0
26-27	4450.0
28-29	8928.0
30-31	18559.0
32-33	19013.0
34-35	21727.0
36-37	22277.0
38-39	27965.0
40-41	40243.0
42-43	56674.0
44-45	74895.0
46-47	71482.0
48-49	46972.0
50-51	38307.0
52-53	32302.0
54-55	33443.0
56-57	39780.0
58-59	58443.0
60-61	35610.0
62-63	38044.0
64-65	50304.0
66-67	75158.0
68-69	45517.0
70-71	46426.0
72-73	67528.0
74-75	48576.0
76-77	26920.0
78-79	41011.0
80-81	70381.0
82-83	63743.0
84-85	88846.0
86-87	101389.0
88-89	110811.0
90-91	111764.0
92-93	111800.0
94-95	121161.0
96-97	245612.0
98-99	410373.0
100-101	4284926.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.18634196024885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.23785422536943	72.51946461204801
2	7.815829212777175	12.847088247684152
3	2.167972738807821	5.345332466164705
4	0.8183017779678121	2.6901291900296895
5	0.3794740752315151	1.559379305601325
6	0.21035738023100956	1.0373102151316707
7	0.11187146213541577	0.6436014369858017
8	0.07311226878466762	0.48070639390610564
9	0.0453208627487468	0.3352280331421808
>10	0.1354726673515732	1.6390069978777222
>50	0.002070391825429494	0.10974074024703824
>100	0.0018518879689327365	0.31957974812592704
>500	2.235838704576594E-4	0.1341676897027436
>1k	2.874649300332129E-4	0.33926492335290337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.4670965455157901E-5	0.0
5	0.0	0.0	0.0	1.4670965455157901E-5	0.0
6	0.0	0.0	0.0	1.4670965455157901E-5	4.40128963654737E-5
7	1.4670965455157901E-5	0.0	0.0	4.40128963654737E-5	4.40128963654737E-5
8	1.4670965455157901E-5	0.0	0.0	4.40128963654737E-5	4.40128963654737E-5
9	1.4670965455157901E-5	0.0	0.0	7.33548272757895E-5	5.8683861820631604E-5
10-11	1.4670965455157901E-5	0.0	0.0	7.33548272757895E-5	5.8683861820631604E-5
12-13	2.9341930910315802E-5	0.0	0.0	7.33548272757895E-5	5.8683861820631604E-5
14-15	2.9341930910315802E-5	0.0	0.0	8.069031000336845E-5	8.80257927309474E-5
16-17	3.667741363789475E-5	0.0	0.0	1.1003224091368426E-4	1.1736772364126321E-4
18-19	4.40128963654737E-5	0.0	0.0	1.8338706818947376E-4	1.320386890964211E-4
20-21	4.40128963654737E-5	0.0	0.0	3.1542575728589484E-4	1.760515854618948E-4
22-23	1.9072255091705272E-4	0.0	0.0	6.675289282096845E-4	1.760515854618948E-4
24-25	2.3473544728252641E-4	0.0	0.0	0.0018852190609877904	1.907225509170527E-4
26-27	2.7874834364800013E-4	0.0	0.0	0.004716715393833265	2.0539351637221062E-4
28-29	3.374322054686317E-4	0.0	0.0	0.019732448537187378	2.2006448182736852E-4
30-31	3.961160672892633E-4	0.0	0.0	0.03121981448857601	2.2006448182736852E-4
32-33	3.961160672892633E-4	0.0	0.0	0.04151883223809686	2.3473544728252641E-4
34-35	3.961160672892633E-4	0.0	0.0	0.05247804343309981	2.860838263755791E-4
36-37	4.4746444638231594E-4	0.0	0.0	0.06090651308708803	3.227612400134738E-4
38-39	4.547999291098949E-4	0.0	0.0	0.0701492213238375	3.227612400134738E-4
40-41	4.768063772926318E-4	0.0	0.0	0.07887111028692886	3.227612400134738E-4
42-43	4.841418600202107E-4	0.0	0.0	0.08526031574265014	3.227612400134738E-4
44-45	4.988128254753686E-4	0.0	0.0	0.0906739019956034	3.741096191065265E-4
46-47	4.988128254753686E-4	0.0	0.0	0.09714379776132803	4.547999291098949E-4
48-49	5.061483082029475E-4	0.0	0.0	0.10357701611341479	4.768063772926318E-4
50-51	5.574966872960003E-4	0.0	0.0	0.11094184077190404	4.841418600202107E-4
52-53	5.86838618206316E-4	0.0	0.0	0.139740945960379	4.841418600202107E-4
54-55	5.94174100933895E-4	0.0	0.0	0.16652279339876974	4.841418600202107E-4
56-57	6.161805491166319E-4	0.0	0.0	0.19378144721445312	4.841418600202107E-4
58-59	6.601934454821055E-4	0.0	0.0	0.2196243528637138	4.841418600202107E-4
60-61	6.748644109372634E-4	0.0	0.0	0.23832249833631253	4.988128254753686E-4
62-63	6.895353763924214E-4	0.0	0.0	0.24518117468659884	4.988128254753686E-4
64-65	7.262127900303161E-4	0.0	0.0	0.24737448402214496	4.988128254753686E-4
66-67	7.33548272757895E-4	0.0	0.0	0.25126962535048936	5.061483082029475E-4
68-69	7.33548272757895E-4	0.0	0.0	0.25538483116066113	5.281547563856844E-4
70-71	7.33548272757895E-4	0.0	0.0	0.2575341275998418	5.281547563856844E-4
72-73	7.40883755485474E-4	0.0	0.0	0.2597641143490258	5.648321700235791E-4
74-75	7.48219238213053E-4	0.0	0.0	0.2621188043045787	6.455224800269476E-4
76-77	7.48219238213053E-4	0.0	0.0	0.26375461695282876	6.455224800269476E-4
78-79	7.48219238213053E-4	0.0	0.0	0.2642974426746696	6.601934454821055E-4
80-81	8.069031000336846E-4	0.0	0.0	0.2646275393974106	6.895353763924214E-4
82-83	8.215740654888425E-4	0.0	0.0	0.26529506832562033	6.968708591200002E-4
84-85	8.215740654888425E-4	0.0	0.0	0.26587457146109905	7.775611691233687E-4
86-87	8.215740654888425E-4	0.0	0.0	0.2663220359074814	8.069031000336846E-4
88	8.215740654888425E-4	0.0	0.0	0.266380719769302	9.389417891301057E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20935	0.0	54.549217	1
GTATCAA	39505	0.0	39.101887	1
TATCAAC	50770	0.0	30.334475	2
ATCAACG	50615	0.0	30.260733	3
TCAACGC	51525	0.0	29.783144	4
CAACGCA	51980	0.0	29.449427	5
AACGCAG	52165	0.0	29.417742	6
GTGGTAT	7910	0.0	29.109116	1
TGGTATC	7645	0.0	28.959742	2
CGCAGAG	57160	0.0	26.796963	8
ACGCAGA	58540	0.0	26.127708	7
GCAGAGT	59345	0.0	24.866272	9
GAGTACT	35210	0.0	24.441126	12-13
TACATGG	42010	0.0	23.931307	2
GTACATG	43225	0.0	23.502531	1
CAGAGTA	57085	0.0	22.63018	10-11
ACATGGG	43805	0.0	22.498207	3
GTACTTT	38990	0.0	22.083967	14-15
AGAGTAC	55125	0.0	21.521399	10-11
AGTACTT	37005	0.0	21.290958	12-13
>>END_MODULE