FastQCFastQC Report
Fri 27 May 2016
SRR2139413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5685938
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170550.045.04311
GTATCAA294950.034.600811
TATCAAC372450.027.2118912
ATCAACG375000.026.8058643
AACGCAG377250.026.7116536
TCAACGC382700.026.2990064
CAACGCA383100.026.1956045
CGCAGAG406900.024.6347968
ACGCAGA420500.023.836167
ACCGAGG48400.023.45335890-91
AGACCGA47300.023.42039988-89
GACCGAG47100.023.3335690-91
CGAGACC47950.023.0281686-87
GCAGAGT426300.022.766249
GAGTACT273050.022.70406212-13
GAGACCG48250.022.41262488-89
GTACATG263400.021.9918441
TACATGG261600.021.7313462
ACATGGG264400.021.0780833
CAGAGTA409700.020.61791810-11
Produced by FastQC (version 0.11.5)