FastQCFastQC Report
Fri 27 May 2016
SRR2139412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6573017
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195950.046.6309471
ATCTCGT35300.043.4739794
GTATCAA343550.035.901431
TATCTCG35800.035.6012394
ATCAACG422300.028.8291453
ACGCAGA435150.028.0762317
AACGCAG438800.027.713476
TACGCTA95000.027.47094594
TATCAAC448100.027.4314652
CAACGCA451800.026.8948125
TCAACGC454500.026.8329774
CGCAGAG486050.025.7588568
CGCTATC53750.023.51558590-91
GTACATG341400.023.279181
TACATGG337650.023.1778952
GCAGAGT512150.022.8726279
GAGTACT312800.022.85780512-13
ACGCTAT74000.022.612994
ACATGGG335400.022.5211163
AGTACTT332400.021.01829312-13
Produced by FastQC (version 0.11.5)