##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139412_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6573017
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84577888662086	33.0	33.0	33.0	33.0	33.0
2	32.90222419932886	33.0	33.0	33.0	33.0	33.0
3	33.53334899331616	33.0	33.0	33.0	33.0	37.0
4	36.70793016357633	37.0	37.0	37.0	37.0	37.0
5	36.72674541994947	37.0	37.0	37.0	37.0	37.0
6	36.699391770932586	37.0	37.0	37.0	37.0	37.0
7	36.68714457911793	37.0	37.0	37.0	37.0	37.0
8	36.671695204804735	37.0	37.0	37.0	37.0	37.0
9	36.69736393500884	37.0	37.0	37.0	37.0	37.0
10-11	36.65695912546704	37.0	37.0	37.0	37.0	37.0
12-13	36.750471815301864	37.0	37.0	37.0	37.0	38.5
14-15	35.29195786348947	40.0	37.0	40.0	19.5	40.0
16-17	36.26326981962773	40.0	37.0	40.0	24.0	40.0
18-19	38.32997944475117	40.0	37.0	40.0	37.0	40.0
20-21	38.647324254011785	40.0	37.0	40.0	37.0	40.0
22-23	38.68586746858298	40.0	37.0	40.0	37.0	40.0
24-25	38.70119524979934	40.0	37.0	40.0	37.0	40.0
26-27	38.64318847328953	40.0	37.0	40.0	37.0	40.0
28-29	38.56763363156248	40.0	37.0	40.0	37.0	40.0
30-31	38.397932338033655	40.0	37.0	40.0	37.0	40.0
32-33	38.20869560150008	40.0	37.0	40.0	37.0	40.0
34-35	38.125255456308956	40.0	37.0	40.0	37.0	40.0
36-37	38.06300970682162	40.0	37.0	40.0	37.0	40.0
38-39	38.05390450029853	40.0	37.0	40.0	37.0	40.0
40-41	38.00954418558767	40.0	37.0	40.0	35.0	40.0
42-43	37.953796635947	40.0	37.0	40.0	33.0	40.0
44-45	37.89081357688234	40.0	37.0	40.0	33.0	40.0
46-47	37.73001062827705	40.0	37.0	40.0	33.0	40.0
48-49	37.5219706022807	40.0	37.0	40.0	33.0	40.0
50-51	37.43858778323613	40.0	37.0	40.0	33.0	40.0
52-53	37.35845963084702	40.0	37.0	40.0	33.0	40.0
54-55	37.143014567497275	37.0	37.0	40.0	33.0	40.0
56-57	37.04116822455774	37.0	37.0	40.0	33.0	40.0
58-59	36.98148661034959	37.0	37.0	40.0	33.0	40.0
60-61	36.85973960857693	37.0	37.0	40.0	33.0	40.0
62-63	36.6764088289143	37.0	37.0	40.0	33.0	40.0
64-65	36.40269344592433	37.0	37.0	40.0	33.0	40.0
66-67	36.08139019990824	37.0	37.0	40.0	33.0	40.0
68-69	35.80286358123257	37.0	33.0	37.0	33.0	40.0
70-71	35.580902225723094	37.0	33.0	37.0	33.0	40.0
72-73	35.36044714734744	37.0	33.0	37.0	33.0	40.0
74-75	34.70871122632094	37.0	33.0	37.0	30.0	38.5
76-77	32.482657066209036	33.0	30.0	37.0	27.0	37.0
78-79	34.38882446402816	37.0	33.0	37.0	27.0	37.0
80-81	34.682027851264486	37.0	33.0	37.0	33.0	37.0
82-83	34.66523122481017	37.0	33.0	37.0	33.0	37.0
84-85	34.63695542100352	37.0	33.0	37.0	33.0	37.0
86-87	34.466819385352345	37.0	33.0	37.0	33.0	37.0
88-89	34.2503080327631	37.0	33.0	37.0	27.0	37.0
90-91	34.12160216968398	37.0	33.0	37.0	27.0	37.0
92-93	34.15031735521408	37.0	33.0	37.0	27.0	37.0
94-95	34.08108023546977	37.0	33.0	37.0	27.0	37.0
96-97	33.99689495946527	37.0	33.0	37.0	27.0	37.0
98-99	33.9648885169923	33.0	33.0	37.0	27.0	37.0
100	33.91122928304779	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	0.0
16	2.0
17	3.0
18	6.0
19	4.0
20	35.0
21	149.0
22	580.0
23	1765.0
24	4189.0
25	8989.0
26	16690.0
27	27626.0
28	43209.0
29	63313.0
30	90502.0
31	125995.0
32	177545.0
33	254964.0
34	379221.0
35	632730.0
36	1171113.0
37	2174062.0
38	1367194.0
39	33129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.94453065571276	0.0	23.568385820781987	28.487083523505248
2	16.08605804478622	23.472244387953737	38.486121699526805	21.955575867733238
3	16.67789456004002	26.007452400121956	32.64062708240908	24.674025957428942
4	13.101194115729225	23.207582678239906	36.97888370588626	26.712339500144605
5	17.294628633605168	36.838753424434536	31.51208515220563	14.354532789754668
6	27.39981326642123	37.591215945349774	20.173798847740933	14.835171940488065
7	26.136602562678707	33.679361798007164	21.980427100946514	18.20360853836761
8	24.727770138711183	31.160251321403	22.427611518623788	21.68436702126203
9	25.62777847086352	19.64636398767051	22.475239089224477	32.25061845224149
10-11	25.310890876400897	25.831226950301733	27.789900711801003	21.067981461496363
12-13	26.103892143438735	24.70599267497568	27.79738470405621	21.392730477529376
14-15	23.562943854510696	26.094611348378177	26.935619267038806	23.40682553007232
16-17	21.2086398072605	28.446564188104183	27.982310102042945	22.362485902592372
18-19	21.04824322287948	27.09764230775931	30.807369797413408	21.046744671947803
20-21	22.575432214921943	26.22227604791731	30.797998556443545	20.4042931807172
22-23	22.18768089433245	26.17179962736148	30.509763652709665	21.130755825596406
24-25	21.587630415010253	26.566934440508405	30.92275981746713	20.922675327014208
26-27	21.580002292651216	26.90051455167053	30.483479010625793	21.036004145052463
28-29	21.311077427460017	27.09343696772747	30.549076203933488	21.04640940087903
30-31	21.830655873104625	26.79618656110985	30.38638013479305	20.98677743099248
32-33	21.4801953895014	27.121148546574204	30.21356062401525	21.185095439909144
34-35	21.63564878107194	27.240867922529617	30.174025114700292	20.949458181698155
36-37	21.92815617100828	27.148952386000747	29.835934519271127	21.086956923719846
38-39	21.375767602780137	27.50806457726978	29.889244254457704	21.22692356549238
40-41	21.960404751156165	27.254254283363444	29.568025535730474	21.217315429749917
42-43	22.03887676337045	27.36001563256336	29.118201453543424	21.482906150522766
44-45	21.893088510988708	28.056253873056043	28.532086754557547	21.51857086139771
46-47	22.244679285433012	27.957738550781176	27.73999525022704	22.057586913558776
48-49	22.281707331588535	28.15423145925145	27.581465401126117	21.982595808033896
50-51	22.303809633946113	28.223192777578504	27.16511266739711	22.307884921078266
52-53	22.427710410547324	28.02712912144244	27.116843112636165	22.42831735537407
54-55	22.48940819716085	28.06302297566581	27.116998582383	22.330570244790344
56-57	22.535439830291622	28.16011688086571	26.922500392079172	22.38194289676349
58-59	22.291296794405895	27.842637859596593	27.321468431700204	22.54459691429731
60-61	22.357086595638048	27.907876710842245	27.48055639122347	22.254480302296237
62-63	22.133329671289744	28.07832605740877	27.300745582526524	22.487598688774963
64-65	22.021404916040925	28.12173554832095	27.12809045367291	22.72876908196522
66-67	22.145461970231764	28.424648830361853	26.98527318231353	22.44461601709285
68-69	22.33513584050848	28.433573139322892	26.883912684197064	22.347378335971555
70-71	22.188833605139504	27.781971425451058	26.62960433831903	23.399590631090405
72-73	22.299929733700292	27.9987994845012	26.635702367120334	23.065568414678182
74-75	22.46409690913839	27.901025475947954	26.986187532444706	22.648690082468946
76-77	22.703976617260828	27.36657444059104	26.448069687417142	23.48137925473099
78-79	22.467057439249	27.63234821451507	26.42425841058606	23.47633593564987
80-81	22.76534104134635	27.979682030711466	26.34676542428466	22.908211503657526
82-83	22.97612983878377	27.455483528387603	26.492847721111335	23.07553891171729
84-85	22.90614520148467	27.798692030475024	26.3219635163635	22.9731992516768
86-87	22.894427563017185	27.91043528923847	26.364834833921286	22.830302313823058
88-89	23.16014677072669	27.74586253148557	26.520618351606647	22.573372346181095
90-91	23.15132265972182	27.51115953187357	26.499689347714554	22.83782846069006
92-93	22.943981321356304	27.17080903272131	26.79703693419179	23.088172711730593
94-95	22.462206661303245	27.418105792611975	27.286128970764995	22.83355857531979
96-97	22.6388997860908	27.647206627887982	27.013085601876856	22.700807984144365
98-99	23.878978760472318	29.436209925206185	26.663637534612395	20.021173779709102
100	22.8703216555658	29.700111663057115	21.151778520802154	26.277788160574932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1908.0
1	1969.5
2	3899.0
3	6090.5
4	9320.5
5	17301.0
6	22998.5
7	24956.5
8	24764.0
9	22170.5
10	19127.0
11	16497.0
12	18486.0
13	26091.0
14	44154.5
15	71425.0
16	96082.0
17	110592.5
18	109265.0
19	95580.0
20	78566.5
21	61749.0
22	48882.5
23	42868.5
24	42222.5
25	46034.5
26	53253.5
27	61975.5
28	70884.0
29	82594.0
30	94646.5
31	105083.0
32	118132.0
33	130903.5
34	144115.0
35	158539.0
36	173302.5
37	188282.0
38	193648.0
39	193719.5
40	198258.0
41	202285.0
42	206315.5
43	213850.0
44	224200.5
45	237133.0
46	246419.5
47	253833.5
48	259051.5
49	258563.5
50	256176.0
51	254735.5
52	250911.5
53	243335.5
54	236703.5
55	223560.0
56	202637.5
57	177342.0
58	153303.0
59	131764.5
60	108791.0
61	87084.0
62	68359.5
63	51040.5
64	34999.0
65	23897.5
66	17582.0
67	13973.0
68	11016.5
69	8387.5
70	6191.0
71	4002.0
72	2779.0
73	1991.0
74	1442.0
75	1068.0
76	755.0
77	565.5
78	370.5
79	236.0
80	169.0
81	121.5
82	98.0
83	70.5
84	51.0
85	41.0
86	32.0
87	22.0
88	17.0
89	14.0
90	8.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	2.586331360469629E-4
2	8.82395405336697E-4
3	0.001536585102396662
4	5.476936998641567E-4
5	5.172662720939258E-4
6	2.4341942216184743E-4
7	7.758994081408887E-4
8	0.0012018833969241218
9	0.002388553079963128
10-11	0.008124123214651658
12-13	0.2232916786918397
14-15	6.891500508822661
16-17	0.0
18-19	7.606856942557732E-6
20-21	3.0429089722909664E-5
22-23	3.2716848987014073E-4
24-25	0.010990162662779418
26-27	0.022807057623040076
28-29	0.00789604656533407
30-31	6.870726217439246E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.828807160665443E-6
44-45	1.7395857524447702E-4
46-47	0.001865604270424223
48-49	0.03389876878991837
50-51	0.07519330817096807
52-53	0.054226525222466064
54-55	0.03572803291553006
56-57	0.005216444478046858
58-59	0.013540305389786884
60-61	0.0398429744570237
62-63	0.031886678148453626
64-65	3.564389810835287E-4
66-67	0.006765518795092011
68-69	0.0423319097519416
70-71	0.08718915347329859
72-73	0.0747636747328699
74-75	0.037071018142299135
76-77	0.00815334186224488
78-79	9.439885312654902E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	6.876987203695182E-5
90-91	0.002319670707178226
92-93	0.003707083702972024
94-95	9.578701347039086E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	202.0
20-21	864.0
22-23	1869.0
24-25	3013.0
26-27	4955.0
28-29	9121.0
30-31	14705.0
32-33	18800.0
34-35	24005.0
36-37	25415.0
38-39	30373.0
40-41	39846.0
42-43	57533.0
44-45	78493.0
46-47	71689.0
48-49	55044.0
50-51	32238.0
52-53	21606.0
54-55	20704.0
56-57	19027.0
58-59	36296.0
60-61	59131.0
62-63	45059.0
64-65	55871.0
66-67	68659.0
68-69	46959.0
70-71	71589.0
72-73	50348.0
74-75	41615.0
76-77	44123.0
78-79	54786.0
80-81	66915.0
82-83	83153.0
84-85	100119.0
86-87	102312.0
88-89	109444.0
90-91	110226.0
92-93	114631.0
94-95	135296.0
96-97	340865.0
98-99	1122433.0
100-101	3183685.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.25201957152865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.51984199930158	68.56872211454214
2	8.472033816727606	13.428515797078974
3	2.565444533530687	6.099499811431356
4	1.0812569433588555	3.4276718574770904
5	0.5173019545377558	2.0498612312708118
6	0.28332148801917	1.347228006811793
7	0.17474685946472535	0.9694329068453104
8	0.11345800117589218	0.7193420583790662
9	0.07230177756978771	0.5157055701915414
>10	0.19617326146154743	2.1888774892468015
>50	0.002354533258196215	0.13045513091117514
>100	0.0013924468684994695	0.24699339612248233
>500	1.8619236398425336E-4	0.10069576864124585
>1k	1.8619236166541345E-4	0.20699886105021253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.042742777023093E-5	0.0
5	0.0	0.0	0.0	3.042742777023093E-5	0.0
6	0.0	0.0	0.0	4.5641141655346394E-5	0.0
7	0.0	0.0	0.0	4.5641141655346394E-5	0.0
8	0.0	0.0	0.0	4.5641141655346394E-5	0.0
9	0.0	0.0	0.0	4.5641141655346394E-5	0.0
10-11	1.5213713885115466E-5	0.0	0.0	7.606856942557733E-5	0.0
12-13	6.085485554046186E-5	0.0	0.0	7.606856942557733E-5	0.0
14-15	1.0649599719580826E-4	0.0	0.0	7.606856942557733E-5	0.0
16-17	1.0649599719580826E-4	0.0	0.0	2.2059885133417424E-4	0.0
18-19	1.369234249660392E-4	0.0	0.0	4.640182734960217E-4	0.0
20-21	1.673508527362701E-4	0.0	0.0	8.367542636813505E-4	0.0
22-23	1.8256456662138558E-4	0.0	0.0	0.0012399176816369104	0.0
24-25	2.738468499320784E-4	0.0	0.0	0.0020766719453182607	0.0
26-27	2.738468499320784E-4	0.0	0.0	0.004343515314200465	0.0
28-29	3.3470170547254027E-4	0.0	0.0	0.013578239642465552	0.0
30-31	3.499154193576557E-4	0.0	0.0	0.020393983462997284	0.0
32-33	3.5752227630021343E-4	0.0	0.0	0.026867418721113912	0.0
34-35	3.803428471278866E-4	0.0	0.0	0.03484701165385697	0.0
36-37	4.2598398878323303E-4	0.0	0.0	0.04490327653191829	0.0
38-39	5.324799859790412E-4	0.0	0.0	0.06990701530210557	0.0
40-41	5.629074137492721E-4	0.0	0.0	0.09560297805406559	0.0
42-43	6.237622692897341E-4	0.0	0.0	0.11716081062927419	0.0
44-45	6.694034109450804E-4	0.0	0.0	0.15364329652578107	0.0
46-47	8.139336928536774E-4	0.0	0.0	0.19475075144336307	0.0
48-49	8.823954053366969E-4	0.0	0.0	0.2324503344506792	0.0
50-51	8.823954053366969E-4	0.0	0.0	0.293099804853692	0.0
52-53	8.823954053366969E-4	0.0	0.0	0.6012307590258781	0.0
54-55	9.280365469920434E-4	0.0	0.0	0.9008800677071123	0.0
56-57	9.736776886473898E-4	0.0	0.0	1.2264839722763534	0.0
58-59	9.888914025325051E-4	0.0	0.0	1.810249387762119	0.0
60-61	0.0010041051164176207	0.0	0.0	1.9895582196120898	0.0
62-63	0.0010345325441878516	0.0	0.0	1.9960468685840915	0.0
64-65	0.0010345325441878516	0.0	0.0	1.9996753393456916	0.0
66-67	0.001080173685843198	0.0	0.0	2.0034559472461426	0.0
68-69	0.0011258148274985445	0.0	0.0	2.0077918557034007	0.0
70-71	0.0011334216844411022	0.0	0.0	2.0104770762041237	0.0
72-73	0.0011410285413836598	0.0	0.0	2.01323075841733	0.0
74-75	0.0011486353983262176	0.0	0.0	2.0148814463738645	0.0
76-77	0.0011562422552687753	0.0	0.0	2.0162582874804675	0.0
78-79	0.0012399176816369104	0.0	0.0	2.0173080337385407	0.0
80-81	0.0013235931080050455	0.0	0.0	2.0177340177273235	0.0
82-83	0.0013388068218901609	0.0	0.0	2.018547951420177	0.0
84-85	0.0013844479635455073	0.0	0.0	2.0192934234005477	0.0
86-87	0.0013844479635455073	0.0	0.0	2.0197878691018145	0.0
88	0.0013844479635455073	0.0	0.0	2.0198182965295843	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19595	0.0	46.630947	1
ATCTCGT	3530	0.0	43.47397	94
GTATCAA	34355	0.0	35.90143	1
TATCTCG	3580	0.0	35.60123	94
ATCAACG	42230	0.0	28.829145	3
ACGCAGA	43515	0.0	28.076231	7
AACGCAG	43880	0.0	27.71347	6
TACGCTA	9500	0.0	27.470945	94
TATCAAC	44810	0.0	27.431465	2
CAACGCA	45180	0.0	26.894812	5
TCAACGC	45450	0.0	26.832977	4
CGCAGAG	48605	0.0	25.758856	8
CGCTATC	5375	0.0	23.515585	90-91
GTACATG	34140	0.0	23.27918	1
TACATGG	33765	0.0	23.177895	2
GCAGAGT	51215	0.0	22.872627	9
GAGTACT	31280	0.0	22.857805	12-13
ACGCTAT	7400	0.0	22.6129	94
ACATGGG	33540	0.0	22.521116	3
AGTACTT	33240	0.0	21.018293	12-13
>>END_MODULE