FastQCFastQC Report
Fri 27 May 2016
SRR2139411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9893307
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174750.048.194071
GTATCAA315550.037.432211
ATCAACG384050.030.1744423
TCAACGC389950.029.70714
TATCAAC394800.029.6836192
CAACGCA390900.029.6025875
AACGCAG396950.029.2401166
CTTATAC142800.028.2794651
ACGCAGA438450.026.4052527
CGCAGAG440450.026.275798
CGCTACG3107.094059E-1126.0631994
TATACAC172600.025.616933
GCAGAGT498200.023.1707979
TACATGG307950.020.948812
TTATACA205800.020.7887862
GAGTACT346300.020.6061212-13
ACATGGG309300.020.3943793
GTACATG331750.019.9663451
CAGAGTA498550.019.91492310-11
ACACATC210750.019.5237246
Produced by FastQC (version 0.11.5)