##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9893307
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69845037660309	33.0	33.0	33.0	33.0	33.0
2	32.70185965117629	33.0	33.0	33.0	33.0	33.0
3	32.71011270548867	33.0	33.0	33.0	33.0	33.0
4	36.641156895262625	37.0	37.0	37.0	37.0	37.0
5	36.653451874080126	37.0	37.0	37.0	37.0	37.0
6	36.59424265313914	37.0	37.0	37.0	37.0	37.0
7	36.57247925289289	37.0	37.0	37.0	37.0	37.0
8	36.572744684866244	37.0	37.0	37.0	37.0	37.0
9	36.61210179771031	37.0	37.0	37.0	37.0	37.0
10-11	36.59491613875926	37.0	37.0	37.0	37.0	37.0
12-13	36.58368152327629	37.0	37.0	37.0	37.0	37.0
14-15	38.687399824952365	40.0	37.0	40.0	37.0	40.0
16-17	38.62243358060151	40.0	37.0	40.0	37.0	40.0
18-19	38.65460568442887	40.0	37.0	40.0	37.0	40.0
20-21	38.6764911943593	40.0	37.0	40.0	37.0	40.0
22-23	38.6725943303377	40.0	37.0	40.0	37.0	40.0
24-25	38.67498340667428	40.0	37.0	40.0	37.0	40.0
26-27	38.58688279901587	40.0	37.0	40.0	37.0	40.0
28-29	38.464648600808474	40.0	37.0	40.0	37.0	40.0
30-31	38.323828208691566	40.0	37.0	40.0	37.0	40.0
32-33	38.208378647970676	40.0	37.0	40.0	37.0	40.0
34-35	38.18203444207062	40.0	37.0	40.0	37.0	40.0
36-37	38.16537686025623	40.0	37.0	40.0	37.0	40.0
38-39	38.10378962004367	40.0	37.0	40.0	37.0	40.0
40-41	38.099985735828454	40.0	37.0	40.0	37.0	40.0
42-43	38.05948254811717	40.0	37.0	40.0	35.0	40.0
44-45	37.96314414555112	40.0	37.0	40.0	33.0	40.0
46-47	37.86948014421901	40.0	37.0	40.0	33.0	40.0
48-49	37.81347336557461	40.0	37.0	40.0	33.0	40.0
50-51	37.77684853513573	40.0	37.0	40.0	33.0	40.0
52-53	37.71989850794986	40.0	37.0	40.0	33.0	40.0
54-55	37.61134962204517	40.0	37.0	40.0	33.0	40.0
56-57	37.434452938977486	40.0	37.0	40.0	33.0	40.0
58-59	37.249098474410715	38.5	37.0	40.0	33.0	40.0
60-61	37.08442387785425	37.0	37.0	40.0	33.0	40.0
62-63	36.95601755502435	37.0	37.0	40.0	33.0	40.0
64-65	36.798398186287656	37.0	37.0	40.0	33.0	40.0
66-67	36.60038195574326	37.0	37.0	40.0	33.0	40.0
68-69	36.399448171893	37.0	37.0	40.0	33.0	40.0
70-71	36.1898114691583	37.0	37.0	38.5	33.0	40.0
72-73	35.90604927516708	37.0	37.0	37.0	33.0	40.0
74-75	35.55234674170906	37.0	33.0	37.0	33.0	40.0
76-77	33.447697768705325	35.0	33.0	37.0	27.0	37.0
78-79	35.08924583390089	37.0	33.0	37.0	33.0	37.0
80-81	35.2963483903136	37.0	33.0	37.0	33.0	37.0
82-83	35.08951473350235	37.0	33.0	37.0	33.0	37.0
84-85	34.94128112217311	37.0	33.0	37.0	33.0	37.0
86-87	34.840717067322686	37.0	33.0	37.0	33.0	37.0
88-89	34.732652500822795	37.0	33.0	37.0	33.0	37.0
90-91	34.66327559663995	37.0	33.0	37.0	33.0	37.0
92-93	34.62416152774858	37.0	33.0	37.0	33.0	37.0
94-95	34.574797287600234	37.0	33.0	37.0	33.0	37.0
96-97	34.58567655756316	37.0	33.0	37.0	33.0	37.0
98-99	34.61600074793158	37.0	33.0	37.0	33.0	37.0
100	34.56722469593127	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	2.0
18	4.0
19	28.0
20	61.0
21	288.0
22	987.0
23	2933.0
24	6854.0
25	14359.0
26	25344.0
27	40913.0
28	62535.0
29	89012.0
30	122818.0
31	167476.0
32	226521.0
33	310564.0
34	440372.0
35	665158.0
36	1221238.0
37	3008918.0
38	3375793.0
39	111127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91538855511105	20.901150646593702	14.654381997849658	23.529078800445593
2	14.106839923474224	20.84047759573126	43.072060965091254	21.980621515703263
3	17.277248609007273	27.20587298830905	31.390040566872873	24.126837835810804
4	11.73841062447572	18.50849266074529	39.820931464069595	29.932165250709392
5	12.487027846199455	38.087355421195355	36.31113438610568	13.114482346499507
6	28.3209345469619	38.99589894460973	19.123211278089318	13.559955230339057
7	25.099018963022175	33.48453656598345	23.793014812943742	17.62342965805064
8	23.71250583854317	36.752180034441466	21.779886139184804	17.75542798783056
9	25.050056568546797	16.927949370215643	21.837834406634705	36.18415965460285
10-11	23.50695331513415	27.154850465757015	29.44810896058217	19.890087258526666
12-13	24.57946250208995	24.60249492093972	29.44214339275364	21.375899184216692
14-15	21.690618718291063	25.93559463989139	27.539749853107764	24.83403678870978
16-17	20.813136598308333	29.134075188407678	28.223676875689797	21.829111337594192
18-19	20.623093976564157	27.99132787449131	30.426069867234485	20.95950828171005
20-21	21.65809060549829	27.263782969889345	30.29932552596057	20.7788008986518
22-23	21.250054589434882	27.29066872462097	30.271656235367	21.187620450577153
24-25	20.96622240759348	27.55765699155086	30.568467085015893	20.907653515839762
26-27	20.96530871241565	28.004748013510323	30.293038266407635	20.73690500766639
28-29	20.714390643333328	28.00764187084957	30.38340972792104	20.894557757896063
30-31	21.418238043631497	27.610750264701593	30.226504699460442	20.744506992206468
32-33	21.044647692990257	27.904877973566695	30.171389798283627	20.87908453515942
34-35	20.987870084441322	27.77302248717295	30.35032883369694	20.888778594688794
36-37	20.99204544989084	27.844279031855063	30.202683329442078	20.960992188812018
38-39	20.819467779188944	27.715981292621667	30.359520508753608	21.10503041943578
40-41	21.086531214773363	27.611493564024435	30.282383859924945	21.01959136127726
42-43	21.335366317004965	27.645905704409778	29.91376183538838	21.104966143196872
44-45	21.03762196930272	28.181788193862612	29.607174165198067	21.1734156716366
46-47	21.494241330793994	27.722588815513433	28.931961529925783	21.851208323766787
48-49	21.503175561624875	27.83147251203559	28.96579379787444	21.6995581284651
50-51	21.13708384425389	28.04977045810549	29.006082430198337	21.807063267442285
52-53	21.31988919665168	28.225434730879527	28.899346702706286	21.555329369762507
54-55	21.46323960376069	28.07264126747607	28.597482687912525	21.86663644085072
56-57	21.74121736072795	28.25471471413475	28.220312165804412	21.78375575933289
58-59	21.269610060414436	28.519023382044058	28.84010663215266	21.37125992538884
60-61	21.585582906793647	28.76848201240995	28.140703403960522	21.50523167683588
62-63	21.407686384875895	28.860453549927772	28.056113724192166	21.675746341004164
64-65	21.31801772356427	28.818440804843693	28.135116116225444	21.728425355366593
66-67	21.677366906096836	28.666385908521164	27.85578765208319	21.800459533298806
68-69	21.58195359842286	28.366764849879246	28.21831981606359	21.832961735634303
70-71	21.70075033279366	27.863160838160685	28.2984487666942	22.137640062351455
72-73	21.87388227156289	27.949295854185223	28.185064412362728	21.991757461889154
74-75	21.593647352101566	28.215840843922322	28.09497140240041	22.0955404015757
76-77	21.730769171244827	28.196866535406627	27.924420960294817	22.14794333305373
78-79	21.832948691802915	28.144127911241707	27.884137628435596	22.13878576851978
80-81	21.75193018608382	27.998584904318225	28.049535936506594	22.19994897309137
82-83	22.08240679396677	27.83832539930366	28.143045610745883	21.93622219598369
84-85	22.328716256182553	27.82104785136094	27.84584629608321	22.004389596373294
86-87	21.742202300569964	28.08886359663686	27.965561684107577	22.2033724186856
88-89	22.184793108641674	27.841560065363847	27.975213527173942	21.998433298820537
90-91	22.286452060917444	27.780539956334028	28.00553863674316	21.927469346005367
92-93	22.24514250302994	27.775991653276442	27.942809588312397	22.036056255381222
94-95	22.03657479308868	27.76900561898108	27.987475103619385	22.206944484310856
96-97	21.987361395446683	27.94038224467796	28.178856679652426	21.893399680222934
98-99	23.165756729725356	29.78300051944237	28.33322868377129	18.71801406706098
100	22.339138631252457	30.552413845238135	21.952582809629515	25.155864713879893
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1731.0
1	1753.5
2	3364.0
3	5296.5
4	7832.0
5	14148.5
6	19166.0
7	21549.0
8	22106.5
9	20369.5
10	17987.0
11	15915.5
12	17422.0
13	24297.5
14	40547.0
15	63275.0
16	82437.0
17	93697.0
18	95528.5
19	89116.5
20	78127.5
21	66359.5
22	58739.5
23	58414.0
24	64372.5
25	77118.0
26	98322.0
27	122173.5
28	140652.0
29	159995.5
30	176245.0
31	190436.0
32	213538.0
33	240067.5
34	269670.5
35	299303.0
36	332271.0
37	360433.5
38	367609.0
39	372266.5
40	388402.0
41	397938.5
42	400078.5
43	409394.5
44	421213.5
45	433924.5
46	449374.0
47	462905.0
48	459259.5
49	441802.0
50	420029.0
51	388742.5
52	352787.5
53	314180.0
54	276456.0
55	242948.0
56	210509.0
57	177693.5
58	149690.0
59	129476.0
60	107159.0
61	82225.0
62	61608.5
63	45387.0
64	32870.0
65	23622.5
66	17461.0
67	13584.5
68	10382.5
69	7779.5
70	5894.5
71	4260.0
72	3262.5
73	2397.5
74	1622.0
75	1065.5
76	757.5
77	499.0
78	321.0
79	225.0
80	156.5
81	109.0
82	76.5
83	49.5
84	33.5
85	22.0
86	16.5
87	13.0
88	8.5
89	4.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0017183334147014744
3	1.3140196700658333E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	4.548529627150961E-5
12-13	0.007368617995984558
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0109153585843141E-5
24-25	3.791670458337125E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.778664129169739E-4
36-37	0.02500531714906624
38-39	0.12265892510464978
40-41	0.14523933169389877
42-43	0.03160240096800561
44-45	0.002313234626540389
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007351784847131716
64-65	0.07681540967034138
66-67	0.18927011289778753
68-69	0.19712156276314985
70-71	0.07380830785881631
72-73	0.0023751401832578186
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	142.0
20-21	757.0
22-23	1746.0
24-25	2959.0
26-27	4745.0
28-29	10538.0
30-31	23738.0
32-33	29606.0
34-35	39109.0
36-37	53081.0
38-39	59166.0
40-41	71562.0
42-43	83660.0
44-45	96664.0
46-47	100171.0
48-49	82345.0
50-51	68538.0
52-53	64850.0
54-55	70025.0
56-57	76500.0
58-59	85592.0
60-61	91565.0
62-63	97194.0
64-65	103562.0
66-67	117475.0
68-69	94368.0
70-71	89765.0
72-73	90794.0
74-75	86141.0
76-77	80639.0
78-79	85630.0
80-81	91971.0
82-83	86426.0
84-85	94569.0
86-87	107208.0
88-89	114134.0
90-91	114117.0
92-93	115264.0
94-95	127737.0
96-97	314900.0
98-99	586721.0
100-101	6177633.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.96341041581412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.73159891925114	68.48622841480609
2	8.287250242525428	13.08779084238181
3	2.480333581077618	5.875667955922738
4	1.0339470024396475	3.265759260073706
5	0.5357214874336859	2.1151197840398277
6	0.30565949159035005	1.448154952916274
7	0.18161177761053185	1.0038479732264194
8	0.11738890127083305	0.741554239144822
9	0.08183523859968948	0.581579057882102
>10	0.24027995292653756	2.721130509004502
>50	0.002776844670982092	0.14285160017024692
>100	0.0013491890722223802	0.21582931157302201
>500	9.894863579322567E-5	0.05957657690683702
>1k	1.4842289560768374E-4	0.2549095219516221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.0107843615891027E-5	0.0	0.0	0.0
5	0.0	1.0107843615891027E-5	0.0	0.0	0.0
6	0.0	1.0107843615891027E-5	0.0	1.0107843615891027E-5	0.0
7	0.0	1.0107843615891027E-5	0.0	9.097059254301924E-5	0.0
8	0.0	1.0107843615891027E-5	0.0	9.097059254301924E-5	0.0
9	0.0	4.0431374463564106E-5	0.0	9.097059254301924E-5	0.0
10-11	0.0	4.0431374463564106E-5	0.0	9.602451435096475E-5	0.0
12-13	0.0	4.0431374463564106E-5	0.0	1.1118627977480128E-4	0.0
14-15	0.0	5.053921807945513E-5	0.0	1.3140196700658335E-4	0.0
16-17	0.0	5.053921807945513E-5	0.0	1.920490287019295E-4	0.0
18-19	0.0	5.559313988740064E-5	0.0	2.6280393401316665E-4	0.0
20-21	0.0	6.0647061695346156E-5	0.0	3.537745265561859E-4	0.0
22-23	0.0	8.086274892712821E-5	0.0	6.822794440726443E-4	0.0
24-25	0.0	9.097059254301924E-5	0.0	0.0016425245875822917	0.0
26-27	0.0	1.0107843615891025E-4	0.0	0.0035882844836413142	0.0
28-29	0.0	1.0107843615891025E-4	0.0	0.010476779907871048	0.0
30-31	0.0	1.0107843615891025E-4	0.0	0.013236221215009298	0.0
32-33	0.0	1.0107843615891025E-4	0.0	0.018704564611206342	0.0
34-35	0.0	1.1118627977480128E-4	0.0	0.025633491409899643	0.0
36-37	0.0	1.2129412339069231E-4	0.0	0.03624167328477727	0.0
38-39	0.0	1.2129412339069231E-4	0.0	0.06426566970983515	0.0
40-41	0.0	1.3140196700658335E-4	0.0	0.09426069563999176	0.0
42-43	0.0	1.4150981062247437E-4	0.0	0.11303601515650935	0.0
44-45	0.0	1.617254978542564E-4	0.0	0.1347072318689797	0.0
46-47	0.0	1.7183334147014744E-4	0.0	0.15614596817828458	0.0
48-49	0.0	1.7183334147014744E-4	0.0	0.17927271437144324	0.0
50-51	0.0	2.1226471593371155E-4	0.0	0.19872020548841757	0.0
52-53	0.0	2.2237255954960257E-4	0.0	0.266301247904265	0.0
54-55	0.0	2.2237255954960257E-4	0.0	0.3510959479979748	0.0
56-57	0.0	2.2237255954960257E-4	0.0	0.422679696485715	0.0
58-59	0.0	2.324804031654936E-4	0.0	0.44703454567820444	0.0
60-61	0.0	2.4258824678138462E-4	0.0	0.46547125243358967	0.0
62-63	0.0	2.4258824678138462E-4	0.0	0.47884898345922144	0.0
64-65	0.0	2.729117776290577E-4	0.0	0.4837866650655842	0.0
66-67	0.0	2.729117776290577E-4	0.0	0.48915393002562235	0.0
68-69	0.0	2.7796569943700324E-4	0.0	0.4944858175330049	0.0
70-71	0.0	2.8301962124494874E-4	0.0	0.4994133912957518	0.0
72-73	0.0	2.8301962124494874E-4	0.0	0.5033049110878698	0.0
74-75	0.0	3.032353084767308E-4	0.0	0.5066910386991934	0.0
76-77	0.0	3.183970739005673E-4	0.0	0.5099002790472387	0.0
78-79	0.0	3.2345099570851285E-4	0.0	0.5118359310996818	0.0
80-81	0.0	3.2345099570851285E-4	0.0	0.5126142350581054	0.0
82-83	0.0	3.2345099570851285E-4	0.0	0.5131145733170921	0.0
84-85	0.0	3.3355883932440384E-4	0.0	0.5133217841112179	0.0
86-87	0.0	3.3355883932440384E-4	0.0	0.5134734017654562	0.0
88	0.0	3.436666829402949E-4	0.0	0.5134784556872641	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17475	0.0	48.19407	1
GTATCAA	31555	0.0	37.43221	1
ATCAACG	38405	0.0	30.174442	3
TCAACGC	38995	0.0	29.7071	4
TATCAAC	39480	0.0	29.683619	2
CAACGCA	39090	0.0	29.602587	5
AACGCAG	39695	0.0	29.240116	6
CTTATAC	14280	0.0	28.279465	1
ACGCAGA	43845	0.0	26.405252	7
CGCAGAG	44045	0.0	26.27579	8
CGCTACG	310	7.094059E-11	26.06319	94
TATACAC	17260	0.0	25.61693	3
GCAGAGT	49820	0.0	23.170797	9
TACATGG	30795	0.0	20.94881	2
TTATACA	20580	0.0	20.788786	2
GAGTACT	34630	0.0	20.60612	12-13
ACATGGG	30930	0.0	20.394379	3
GTACATG	33175	0.0	19.966345	1
CAGAGTA	49855	0.0	19.914923	10-11
ACACATC	21075	0.0	19.523724	6
>>END_MODULE