FastQCFastQC Report
Fri 27 May 2016
SRR2139410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10166115
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168900.051.6827771
GTATCAA314950.039.470861
ATCAACG385050.031.677313
TCAACGC393450.031.0230244
CAACGCA394350.030.9302645
TATCAAC399350.030.8470462
AACGCAG401150.030.4671156
ACGCAGA444900.027.4808147
CGCAGAG451400.027.08518
GCAGAGT507700.023.9536159
TACATGG284800.021.3761922
CTTATAC108750.021.1422041
CGACGAG9550.020.90962624-25
GTGGTAT74350.020.9073261
GAGTACT377300.020.88310812-13
CGCTACG2857.4479976E-720.66943794
ACATGGG285450.020.629443
CAGAGTA505200.020.60020610-11
GTACATG311300.020.3354241
TATACAC136850.020.2494743
Produced by FastQC (version 0.11.5)