##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139410_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10166115
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69347877729103	33.0	33.0	33.0	33.0	33.0
2	32.696367983246304	33.0	33.0	33.0	33.0	33.0
3	32.70750704669385	33.0	33.0	33.0	33.0	33.0
4	36.639263376422555	37.0	37.0	37.0	37.0	37.0
5	36.650956338778386	37.0	37.0	37.0	37.0	37.0
6	36.59230482834396	37.0	37.0	37.0	37.0	37.0
7	36.570614438258865	37.0	37.0	37.0	37.0	37.0
8	36.568365890017965	37.0	37.0	37.0	37.0	37.0
9	36.608802576008635	37.0	37.0	37.0	37.0	37.0
10-11	36.591510375399054	37.0	37.0	37.0	37.0	37.0
12-13	36.58140184328035	37.0	37.0	37.0	37.0	37.0
14-15	38.68355487814175	40.0	37.0	40.0	37.0	40.0
16-17	38.619977247945755	40.0	37.0	40.0	37.0	40.0
18-19	38.65219093036032	40.0	37.0	40.0	37.0	40.0
20-21	38.671056944320796	40.0	37.0	40.0	37.0	40.0
22-23	38.66821885670283	40.0	37.0	40.0	37.0	40.0
24-25	38.66842611118079	40.0	37.0	40.0	37.0	40.0
26-27	38.582713154895	40.0	37.0	40.0	37.0	40.0
28-29	38.463276707846404	40.0	37.0	40.0	37.0	40.0
30-31	38.3253940263391	40.0	37.0	40.0	37.0	40.0
32-33	38.210507309659675	40.0	37.0	40.0	37.0	40.0
34-35	38.180529533437465	40.0	37.0	40.0	37.0	40.0
36-37	38.16976109592696	40.0	37.0	40.0	37.0	40.0
38-39	38.112252115506266	40.0	37.0	40.0	37.0	40.0
40-41	38.09976061486488	40.0	37.0	40.0	37.0	40.0
42-43	38.050215991213165	40.0	37.0	40.0	35.0	40.0
44-45	37.95319485834344	40.0	37.0	40.0	33.0	40.0
46-47	37.85847492520938	40.0	37.0	40.0	33.0	40.0
48-49	37.79318663112306	40.0	37.0	40.0	33.0	40.0
50-51	37.76632156717325	40.0	37.0	40.0	33.0	40.0
52-53	37.71731094763875	40.0	37.0	40.0	33.0	40.0
54-55	37.612499792205796	40.0	37.0	40.0	33.0	40.0
56-57	37.444103041645945	40.0	37.0	40.0	33.0	40.0
58-59	37.261893102186576	38.5	37.0	40.0	33.0	40.0
60-61	37.1039501718182	37.0	37.0	40.0	33.0	40.0
62-63	36.97610164598176	37.0	37.0	40.0	33.0	40.0
64-65	36.82086272112518	37.0	37.0	40.0	33.0	40.0
66-67	36.62494840438609	37.0	37.0	40.0	33.0	40.0
68-69	36.42551217711929	37.0	37.0	40.0	33.0	40.0
70-71	36.216742088419274	37.0	37.0	40.0	33.0	40.0
72-73	35.932183572257486	37.0	37.0	37.0	33.0	40.0
74-75	35.57264629392046	37.0	33.0	37.0	33.0	40.0
76-77	33.468158735925755	35.0	33.0	37.0	27.0	37.0
78-79	35.104171525110964	37.0	33.0	37.0	33.0	37.0
80-81	35.30519576024892	37.0	33.0	37.0	33.0	37.0
82-83	35.09635535835957	37.0	33.0	37.0	33.0	37.0
84-85	34.940790924494344	37.0	33.0	37.0	33.0	37.0
86-87	34.8314125815202	37.0	33.0	37.0	33.0	37.0
88-89	34.71727393336235	37.0	33.0	37.0	33.0	37.0
90-91	34.64315923885535	37.0	33.0	37.0	33.0	37.0
92-93	34.599147059663615	37.0	33.0	37.0	33.0	37.0
94-95	34.549055162504914	37.0	33.0	37.0	33.0	37.0
96-97	34.561318663985446	37.0	33.0	37.0	33.0	37.0
98-99	34.58909665274585	37.0	33.0	37.0	33.0	37.0
100	34.53787743891509	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	0.0
18	6.0
19	11.0
20	56.0
21	304.0
22	959.0
23	3051.0
24	7310.0
25	14840.0
26	26412.0
27	43851.0
28	65473.0
29	94811.0
30	130049.0
31	176768.0
32	238930.0
33	326724.0
34	463081.0
35	698414.0
36	1276952.0
37	3092499.0
38	3406427.0
39	99185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.172896430937485	20.821001926497978	14.591955727433733	23.414145915130806
2	14.065588286995062	20.96970754572306	43.15355868537766	21.811145481904216
3	17.27487093873345	27.200779610513447	31.394107594041454	24.13024185671165
4	11.635300210552408	18.50603696692394	40.41620619086052	29.442456631663127
5	12.370113853718948	38.29636001560085	36.416625229991986	12.91690090068822
6	28.131444509529945	39.36770339505308	19.25498580332802	13.245866292088964
7	24.94356988879233	33.713488387648574	24.059476014190277	17.28346570936882
8	23.578663038928834	36.92448885341155	21.800707546589823	17.69614056106979
9	25.14926301738668	16.93424675994714	22.017761947410587	35.89872827525559
10-11	23.36783539148731	27.31212734941792	29.657621694673043	19.662415564421725
12-13	24.440066264580224	24.83729960030732	29.615599524264386	21.10703461084807
14-15	21.523079367093526	26.162113058921722	27.888519852470683	24.42628772151407
16-17	20.58440712110772	29.177281586918895	28.58037706636213	21.657934225611257
18-19	20.42999218482183	28.035995067929097	30.80467808990947	20.729334657339603
20-21	21.48357702342421	27.463323525659295	30.637141078920216	20.41595837199628
22-23	21.071125636973974	27.318340305393345	30.597520398219043	21.013013659413637
24-25	20.74249423263393	27.434100054004716	30.9958923060279	20.827513407333452
26-27	20.815855514775073	27.766851543730702	30.661307239773755	20.75598570172047
28-29	20.508469902436456	27.99533578001348	30.640990477053386	20.855203840496678
30-31	21.22032890320279	27.696817852294565	30.48620190538238	20.596651339120264
32-33	21.098776537736793	27.79402512260645	30.45679288736956	20.650405452287206
34-35	20.725891816617928	27.946176453068944	30.665429773652736	20.662501956660392
36-37	21.01947566092981	27.71299526208719	30.509800330675716	20.75772874630728
38-39	20.72195086006218	27.998243455215206	30.553010385670614	20.726795299051997
40-41	21.09560244346441	27.94782859780552	30.283222463566844	20.673346495163226
42-43	21.105921189290548	27.96672503992025	30.01339463661146	20.913959134177748
44-45	21.06202575087398	28.48123867055784	29.577684255722737	20.879051322845445
46-47	21.299600887304805	28.098448677243738	29.0445041034652	21.557446331986256
48-49	21.330772942489833	28.19703587930088	29.129298988864793	21.342892189344497
50-51	21.018116529961688	28.458785104610442	29.039241916633724	21.48385644879415
52-53	21.276526006834885	28.528843779233696	28.789331629764952	21.405298584166463
54-55	21.377229591292117	28.366199649918798	28.74198248764922	21.514588271139857
56-57	21.568004373458844	28.58895065165672	28.39489820775586	21.448146767128577
58-59	21.211818230143095	28.697353765867174	28.865479749750012	21.22534825423972
60-61	21.493029786179992	28.76030674181056	28.475576077404984	21.27108739460446
62-63	21.29937745543805	28.71624483824528	28.40805886762134	21.576318838695325
64-65	21.187727830706518	28.81272863820269	28.489436535312358	21.510106995778436
66-67	21.5408445262561	28.831605609730527	28.19795878061562	21.429591083397753
68-69	21.390854993865375	28.620341572841255	28.483055279798382	21.505748153494988
70-71	21.455308743205855	28.129127887019163	28.45133119549287	21.96423217428211
72-73	21.61647325504595	28.25743472458713	28.38315650014317	21.742935520223746
74-75	21.318448959160254	28.434068522810396	28.515579137445663	21.731903380583688
76-77	21.404911820677924	28.364167645249488	28.325075492704936	21.905845041367648
78-79	21.559738593555423	28.450906824229566	28.033823489556465	21.955531092658543
80-81	21.65484521009638	28.230108612626193	28.24414281205989	21.87090336521754
82-83	21.858053673197432	28.050502583694776	28.389153739870203	21.702290003237586
84-85	22.143817110455338	27.966247883896866	28.057585933738288	21.83234907190951
86-87	21.418780755812417	28.290973892396444	28.234771156558512	22.055474195232627
88-89	22.008299755190063	28.085457371670124	28.16399018220833	21.742252690931487
90-91	21.99371094714797	28.079936111602642	28.298872253265312	21.62748068798408
92-93	21.967242909996827	27.958429875430724	28.130570961769173	21.94375625280328
94-95	21.865847172727364	28.12951996157631	28.157963007286803	21.84666985840952
96-97	21.874088287036315	28.128946676923324	28.407432359138184	21.589532676902177
98-99	22.859795230907924	30.159471996417775	28.60066224620588	18.380070526468423
100	22.098403445969726	31.04977194212172	22.23713195555685	24.614692656351707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1886.0
1	1852.0
2	3389.5
3	5320.5
4	7875.5
5	14155.5
6	19124.5
7	21428.5
8	21886.0
9	20127.5
10	17723.5
11	15652.5
12	17341.0
13	24433.5
14	41160.5
15	64536.0
16	84277.0
17	96661.0
18	99331.5
19	92475.0
20	80814.0
21	68056.0
22	59124.5
23	57560.5
24	62357.5
25	75148.5
26	98747.0
27	126309.5
28	145129.5
29	163706.0
30	180219.5
31	195125.5
32	220458.5
33	247528.0
34	278618.5
35	310255.0
36	345339.0
37	374193.5
38	374496.5
39	371881.5
40	387476.0
41	395508.5
42	393219.5
43	400335.0
44	409167.0
45	413747.0
46	414375.5
47	415324.0
48	413201.0
49	407254.5
50	393845.5
51	365779.5
52	331850.5
53	299932.0
54	269587.0
55	236648.5
56	205214.5
57	176628.0
58	152214.5
59	132903.5
60	108930.0
61	82382.0
62	60905.5
63	43621.0
64	30421.0
65	21080.5
66	15090.5
67	11316.5
68	8303.5
69	5969.0
70	4666.5
71	3486.5
72	2717.5
73	2038.0
74	1363.0
75	898.5
76	591.5
77	386.5
78	240.5
79	160.5
80	117.0
81	94.5
82	82.5
83	55.5
84	34.5
85	27.0
86	22.5
87	18.5
88	12.5
89	8.0
90	5.0
91	4.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001809934276761575
3	1.2787579129293737E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.393789564646869E-5
12-13	0.007918462460831891
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.918907626702329E-6
24-25	3.4930503549283545E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.777766294603578E-4
36-37	0.026218411017504942
38-39	0.12552878203814605
40-41	0.14773710128703424
42-43	0.032771579684346554
44-45	0.002514650864916433
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0074012986232736885
64-65	0.07555654332041853
66-67	0.18310863898011448
68-69	0.19112262135196195
70-71	0.07237289317802839
72-73	0.0024608147267624345
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	133.0
20-21	755.0
22-23	1610.0
24-25	2993.0
26-27	4824.0
28-29	8999.0
30-31	16364.0
32-33	18309.0
34-35	23344.0
36-37	27121.0
38-39	29058.0
40-41	41365.0
42-43	53985.0
44-45	62870.0
46-47	64592.0
48-49	51078.0
50-51	43819.0
52-53	51339.0
54-55	52607.0
56-57	48849.0
58-59	39033.0
60-61	70901.0
62-63	59657.0
64-65	84289.0
66-67	111788.0
68-69	96526.0
70-71	96269.0
72-73	93386.0
74-75	92566.0
76-77	86750.0
78-79	93703.0
80-81	102936.0
82-83	93890.0
84-85	106804.0
86-87	113185.0
88-89	121992.0
90-91	123428.0
92-93	122967.0
94-95	138536.0
96-97	357289.0
98-99	639490.0
100-101	6716716.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.58218158115042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.60555551092112	65.55860196492614
2	8.813300468362877	13.49883553594808
3	2.7604922607088795	6.342135586889036
4	1.1750686711580747	3.5995728937979523
5	0.5787879492278045	2.2162421912372703
6	0.33274979256922366	1.5289623021375855
7	0.20917225984933085	1.1213207589864727
8	0.12789305708572227	0.7835463456565775
9	0.08971597147761429	0.6183580336585173
>10	0.29930502491453315	3.4131903639804513
>50	0.0046937204120812105	0.24842220139243568
>100	0.002640757088567134	0.38219236985576005
>500	3.1227836197593445E-4	0.18177533448975697
>1k	2.838889656528718E-4	0.39153072122230304
>5k	2.838889656498845E-5	0.11531339582157259
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.9509797990677855E-5	0.0	0.0	0.0
4	0.0	2.9509797990677855E-5	0.0	0.0	0.0
5	0.0	3.9346397320903804E-5	0.0	0.0	0.0
6	0.0	3.9346397320903804E-5	0.0	0.0	0.0
7	0.0	3.9346397320903804E-5	0.0	0.0	0.0
8	0.0	3.9346397320903804E-5	0.0	0.0	0.0
9	0.0	4.918299665112976E-5	0.0	0.0	0.0
10-11	0.0	4.918299665112976E-5	0.0	0.0	0.0
12-13	0.0	4.918299665112976E-5	0.0	4.9182996651129755E-6	0.0
14-15	0.0	8.361109430692059E-5	0.0	9.836599330225951E-6	0.0
16-17	0.0	8.852939397203356E-5	0.0	9.836599330225951E-6	0.0
18-19	0.0	9.83659933022595E-5	0.0	1.4754898995338927E-5	0.0
20-21	0.0	1.0820259263248546E-4	0.0	3.442809765579083E-5	0.0
22-23	0.0	1.0820259263248546E-4	0.0	1.8689538727429308E-4	0.0
24-25	0.0	1.1312089229759844E-4	0.0	7.426632494320594E-4	0.0
26-27	0.0	1.1803919196271142E-4	0.0	0.002026339462026546	0.0
28-29	0.0	1.1803919196271142E-4	0.0	0.005862613200814667	0.0
30-31	0.0	1.1803919196271142E-4	0.0	0.006531501955270032	0.0
32-33	0.0	1.1803919196271142E-4	0.0	0.0078102598681994055	0.0
34-35	0.0	1.1803919196271142E-4	0.0	0.009069344582468327	0.0
36-37	0.0	1.1803919196271142E-4	0.0	0.011026827849183293	0.0
38-39	0.0	1.1803919196271142E-4	0.0	0.01507458847357127	0.0
40-41	0.0	1.1803919196271142E-4	0.0	0.01961417906447055	0.0
42-43	0.0	1.1803919196271142E-4	0.0	0.023735714183835223	0.0
44-45	0.0	1.3771239062316333E-4	0.0	0.027960533596167268	0.0
46-47	0.0	1.475489899533893E-4	0.0	0.03234273859778293	0.0
48-49	0.0	1.475489899533893E-4	0.0	0.03871193666410423	0.0
50-51	0.0	1.475489899533893E-4	0.0	0.04445651067295619	0.0
52-53	0.0	1.6722218861384117E-4	0.0	0.06395756884512914	0.0
54-55	0.0	1.7214048827895415E-4	0.0	0.08879006385428456	0.0
56-57	0.0	1.9673198660451904E-4	0.0	0.1077599456626253	0.0
58-59	0.0	2.459149832556488E-4	0.0	0.21570186841285977	0.0
60-61	0.0	2.459149832556488E-4	0.0	0.3163302795610713	0.0
62-63	0.0	2.459149832556488E-4	0.0	0.32007802390588735	0.0
64-65	0.0	2.459149832556488E-4	0.0	0.32357985326744776	0.0
66-67	0.0	2.459149832556488E-4	0.0	0.3280604242623657	0.0
68-69	0.0	2.459149832556488E-4	0.0	0.3335246551903063	0.0
70-71	0.0	2.459149832556488E-4	0.0	0.3389741312192514	0.0
72-73	0.0	2.459149832556488E-4	0.0	0.34340551921751816	0.0
74-75	0.0	2.459149832556488E-4	0.0	0.3466761884948183	0.0
76-77	0.0	2.459149832556488E-4	0.0	0.3498780015768069	0.0
78-79	0.0	2.459149832556488E-4	0.0	0.35186991294117764	0.0
80-81	0.0	2.459149832556488E-4	0.0	0.35268143238592126	0.0
82-83	0.0	2.459149832556488E-4	0.0	0.3532273636487488	0.0
84-85	0.0	2.5575158258587475E-4	0.0	0.3534240956353533	0.0
86-87	0.0	2.5575158258587475E-4	0.0	0.3536109910226276	0.0
88	0.0	2.5575158258587475E-4	0.0	0.35362574592162294	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16890	0.0	51.682777	1
GTATCAA	31495	0.0	39.47086	1
ATCAACG	38505	0.0	31.67731	3
TCAACGC	39345	0.0	31.023024	4
CAACGCA	39435	0.0	30.930264	5
TATCAAC	39935	0.0	30.847046	2
AACGCAG	40115	0.0	30.467115	6
ACGCAGA	44490	0.0	27.480814	7
CGCAGAG	45140	0.0	27.0851	8
GCAGAGT	50770	0.0	23.953615	9
TACATGG	28480	0.0	21.376192	2
CTTATAC	10875	0.0	21.142204	1
CGACGAG	955	0.0	20.909626	24-25
GTGGTAT	7435	0.0	20.907326	1
GAGTACT	37730	0.0	20.883108	12-13
CGCTACG	285	7.4479976E-7	20.669437	94
ACATGGG	28545	0.0	20.62944	3
CAGAGTA	50520	0.0	20.600206	10-11
GTACATG	31130	0.0	20.335424	1
TATACAC	13685	0.0	20.249474	3
>>END_MODULE