FastQCFastQC Report
Fri 27 May 2016
SRR2139409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9584179
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT60600.051.02013894
GGTATCA157200.049.8347431
TATCTCG79850.038.06128794
GTATCAA284800.037.7655261
ATCAACG345650.030.4560323
TCAACGC352100.029.9346754
TATCAAC361650.029.5378552
CAACGCA359400.029.2786945
AACGCAG360550.029.2609846
ACGCAGA403350.026.1026387
CGCAGAG407900.025.822048
CGCTATC125450.025.32753294
GCAGAGT460200.022.746999
TATGCCG27750.021.96695386-87
TACATGG299100.021.8554672
GTACATG316250.021.3924261
ACATGGG301300.021.1664963
CTATCTC142300.021.03404894
GTGGTAT71500.020.8526361
TCGTATG29900.020.80298884-85
Produced by FastQC (version 0.11.5)