##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9584179
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.711881633262486	33.0	33.0	33.0	33.0	33.0
2	32.71367312734873	33.0	33.0	33.0	33.0	33.0
3	32.72596223421954	33.0	33.0	33.0	33.0	33.0
4	36.65904069613057	37.0	37.0	37.0	37.0	37.0
5	36.66975084668181	37.0	37.0	37.0	37.0	37.0
6	36.61377599479309	37.0	37.0	37.0	37.0	37.0
7	36.593119243703605	37.0	37.0	37.0	37.0	37.0
8	36.59161739362339	37.0	37.0	37.0	37.0	37.0
9	36.628948395058146	37.0	37.0	37.0	37.0	37.0
10-11	36.613568152264264	37.0	37.0	37.0	37.0	37.0
12-13	36.603012527207596	37.0	37.0	37.0	37.0	37.0
14-15	38.739049896709986	40.0	37.0	40.0	37.0	40.0
16-17	38.6783998399863	40.0	37.0	40.0	37.0	40.0
18-19	38.705348522810354	40.0	37.0	40.0	37.0	40.0
20-21	38.71969195140832	40.0	37.0	40.0	37.0	40.0
22-23	38.71824362040235	40.0	37.0	40.0	37.0	40.0
24-25	38.71945048674617	40.0	37.0	40.0	37.0	40.0
26-27	38.63926462770209	40.0	37.0	40.0	37.0	40.0
28-29	38.52773863978307	40.0	37.0	40.0	37.0	40.0
30-31	38.39603150604374	40.0	37.0	40.0	37.0	40.0
32-33	38.28822526229669	40.0	37.0	40.0	37.0	40.0
34-35	38.268252222307844	40.0	37.0	40.0	37.0	40.0
36-37	38.25936244245324	40.0	37.0	40.0	37.0	40.0
38-39	38.2143694682738	40.0	37.0	40.0	37.0	40.0
40-41	38.191521076195265	40.0	37.0	40.0	37.0	40.0
42-43	38.13829422265151	40.0	37.0	40.0	37.0	40.0
44-45	38.043383873957	40.0	37.0	40.0	35.0	40.0
46-47	37.94475475702482	40.0	37.0	40.0	33.0	40.0
48-49	37.880798834623384	40.0	37.0	40.0	33.0	40.0
50-51	37.85803635736937	40.0	37.0	40.0	33.0	40.0
52-53	37.79828168194176	40.0	37.0	40.0	33.0	40.0
54-55	37.68664469698204	40.0	37.0	40.0	33.0	40.0
56-57	37.51658022148072	40.0	37.0	40.0	33.0	40.0
58-59	37.333604748896704	38.5	37.0	40.0	33.0	40.0
60-61	37.168318594897386	37.0	37.0	40.0	33.0	40.0
62-63	37.03234711860577	37.0	37.0	40.0	33.0	40.0
64-65	36.86255478549831	37.0	37.0	40.0	33.0	40.0
66-67	36.6478697319217	37.0	37.0	40.0	33.0	40.0
68-69	36.44331096879212	37.0	37.0	40.0	33.0	40.0
70-71	36.224274634692705	37.0	37.0	38.5	33.0	40.0
72-73	35.93965743396056	37.0	37.0	37.0	33.0	40.0
74-75	35.57531306352819	37.0	33.0	37.0	33.0	40.0
76-77	33.45089588727624	35.0	33.0	37.0	27.0	37.0
78-79	35.088024428210595	37.0	33.0	37.0	33.0	37.0
80-81	35.2871446660526	37.0	33.0	37.0	33.0	37.0
82-83	35.088932647574936	37.0	33.0	37.0	33.0	37.0
84-85	34.94010750283077	37.0	33.0	37.0	33.0	37.0
86-87	34.83990786958665	37.0	33.0	37.0	33.0	37.0
88-89	34.73649471911793	37.0	33.0	37.0	33.0	37.0
90-91	34.672308355640254	37.0	33.0	37.0	33.0	37.0
92-93	34.646293337341035	37.0	33.0	37.0	33.0	37.0
94-95	34.58449166414033	37.0	33.0	37.0	33.0	37.0
96-97	34.582913638511364	37.0	33.0	37.0	33.0	37.0
98-99	34.61134101191924	37.0	33.0	37.0	33.0	37.0
100	34.55838126844537	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	6.0
19	13.0
20	69.0
21	248.0
22	895.0
23	2652.0
24	6425.0
25	13046.0
26	23639.0
27	38726.0
28	57857.0
29	84099.0
30	116020.0
31	158688.0
32	213971.0
33	293330.0
34	417365.0
35	636706.0
36	1178360.0
37	2957285.0
38	3287246.0
39	97530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64096284522262	20.48493882313121	14.381880219670379	23.4922181119758
2	14.323527303471693	20.83688221651897	42.593870764412486	22.245719715596856
3	17.643347430896934	26.829179222972233	30.843875552187033	24.6835977939438
4	12.04327590743828	18.017518038584008	39.83347346011312	30.105732593864598
5	12.798520645171656	38.0229373870143	36.02776367467298	13.15077829314105
6	29.001430378126287	38.55712628071742	19.08075798667784	13.360685354478457
7	25.663481452088906	33.17305530291118	23.941163870165614	17.222299374834297
8	24.027086722816843	36.647395671554136	21.530190535882106	17.795327069746925
9	25.502027873227327	16.880006101722433	21.881707342903344	35.7362586821469
10-11	23.830177904797402	27.0157012014915	29.291270992556374	19.86284990115473
12-13	24.771117217817146	24.579380491594183	29.404128689099394	21.245373601489277
14-15	21.853937619487283	25.830444110027578	27.577625584831	24.737992685654138
16-17	21.001762383611574	28.832250524536324	28.298589790528744	21.867397301323358
18-19	20.829363683629033	27.97659037878988	30.377933258550367	20.816112679030724
20-21	21.863944070607282	27.232664516611692	30.24036771511845	20.66302369766258
22-23	21.519217046070395	27.006759805850784	30.139679557894016	21.334343590184808
24-25	21.098596031289667	27.116406941404907	30.47874289852672	21.306254128778704
26-27	21.112361692008285	27.435454965489136	30.202938115142636	21.24924522735994
28-29	20.98848936592614	27.72858844008112	30.165856735301695	21.117065458691044
30-31	21.406838556973184	27.47605120323267	29.998556172045515	21.118554067748633
32-33	21.182214281761887	27.699675585990878	29.87957193615512	21.238538196092108
34-35	21.254505678429908	27.750650924541603	29.90813965453476	21.086703742493732
36-37	21.386767202526553	27.52882322980516	29.906846095894164	21.17756347177413
38-39	21.17665803076583	27.818885865573723	29.884562645745717	21.11989345791474
40-41	21.495522715778677	27.7158010144128	29.682765684430766	21.105910585377757
42-43	21.4203070225132	27.801346639499396	29.311740462069935	21.46660587591747
44-45	21.406126970638415	28.109430648603116	29.041343630546855	21.443098750211607
46-47	21.53639584998649	27.931644173953803	28.75606871312437	21.77589126293534
48-49	21.647977038365173	28.10260500274039	28.59498035345395	21.65443760544049
50-51	21.463421273265826	28.214924452906125	28.400460179501874	21.921194094326175
52-53	21.53796853862581	28.13513426950441	28.18999320410871	22.136903987761066
54-55	21.798184939284802	27.863954752945002	28.34551997254141	21.992340335228786
56-57	21.685787366115598	27.66824105033885	28.394961519140878	22.25101006440468
58-59	21.499983007967057	27.552009432057062	28.647155720625978	22.3008518393499
60-61	21.641035300076013	27.721011045613388	28.721670388048953	21.916283266261647
62-63	21.37468370380232	27.811664086453558	28.63869713395576	22.174955075788365
64-65	21.213485638125007	28.188896550107188	28.373878177729882	22.22373963403792
66-67	21.38856049009712	28.56014668526315	27.893720253244346	22.15757257139538
68-69	21.39117473241612	28.498021890254314	27.88508475379651	22.225718623533055
70-71	21.417670840873335	27.89032025629437	27.830348972454	22.861659930378302
72-73	21.68806538927274	27.64862763254743	27.82924353586438	22.834063442315454
74-75	21.67182918772337	27.710485483288256	27.95357017598151	22.664115153006854
76-77	21.966682107045145	27.590425430394237	27.392877451362054	23.050015011198568
78-79	22.14685996890812	27.66018382622532	27.020620067371855	23.1723361374947
80-81	22.00360658565709	27.801022253687858	27.259712653727558	22.935658506927492
82-83	22.19187995346504	27.52624097449917	27.26951297624626	23.012366095789528
84-85	22.424716060858348	27.836362896809618	27.00720322736772	22.73171781496431
86-87	22.328106944217073	27.929940478688593	27.17049993224201	22.57145264485233
88-89	22.545116141759397	27.73433977483964	27.456254695605235	22.264289387795728
90-91	22.452946438859904	27.445123334354165	27.47263496446541	22.62929526232052
92-93	22.397799284933683	27.15853051990072	27.501029458966997	22.9426407361986
94-95	21.90109477513138	27.42625219374437	27.960785303509727	22.71186772761452
96-97	21.94894939574643	27.43659810380455	28.07350699376463	22.540945506684395
98-99	23.108584108829035	29.240528722781768	28.50226019308655	19.14862697530266
100	22.355123032791514	30.04183934043195	21.977193145696496	25.625844481080044
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1657.0
1	1683.0
2	3067.5
3	4726.0
4	6947.0
5	12611.5
6	17214.5
7	19305.0
8	19622.5
9	18096.0
10	16093.5
11	14314.5
12	15774.0
13	21867.0
14	35706.5
15	55113.5
16	71363.5
17	81107.0
18	83040.0
19	77510.5
20	68099.0
21	57772.0
22	50307.5
23	49044.0
24	53655.5
25	63835.5
26	81582.5
27	103485.0
28	121930.0
29	140713.0
30	158414.0
31	173203.5
32	194864.0
33	221367.5
34	247958.0
35	272212.0
36	300375.0
37	328684.5
38	338978.5
39	344711.5
40	356760.0
41	363795.0
42	368915.0
43	380859.5
44	394174.0
45	404674.0
46	411773.0
47	416005.5
48	416540.5
49	407239.0
50	391194.0
51	369789.0
52	340711.0
53	308601.0
54	277979.0
55	249789.0
56	220326.0
57	189526.5
58	161572.0
59	139186.0
60	114635.0
61	88023.5
62	67621.5
63	51624.5
64	38002.0
65	26833.5
66	19357.0
67	14644.5
68	11264.0
69	8799.0
70	6941.5
71	4739.0
72	3285.0
73	2368.5
74	1644.5
75	1116.0
76	751.5
77	478.5
78	302.0
79	186.5
80	125.5
81	104.0
82	79.0
83	47.0
84	28.5
85	20.0
86	17.0
87	15.5
88	12.0
89	8.0
90	5.0
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0433861888430924E-5
2	0.0018572274161407045
3	1.773756521033257E-4
4	1.0433861888430924E-5
5	1.0433861888430924E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.347089510744739E-5
12-13	0.007569766800056635
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0435099291274144E-5
24-25	2.3483005661909217E-4
26-27	0.0
28-29	1.044612091917716E-5
30-31	1.0458730919810347E-5
32-33	1.0479841553179589E-5
34-35	5.882960702925559E-4
36-37	0.02546798006193889
38-39	0.12163844146940378
40-41	0.1429584327442851
42-43	0.032033738855273566
44-45	0.002467597960406367
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007430733014102107
64-65	0.07404730785022881
66-67	0.18039160922497982
68-69	0.1887167164476993
70-71	0.07096420938335608
72-73	0.002641381424678041
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	118.0
20-21	692.0
22-23	1472.0
24-25	2682.0
26-27	4437.0
28-29	8883.0
30-31	18643.0
32-33	21727.0
34-35	28825.0
36-37	33963.0
38-39	35439.0
40-41	56365.0
42-43	70357.0
44-45	87083.0
46-47	84761.0
48-49	75266.0
50-51	41647.0
52-53	16695.0
54-55	16113.0
56-57	15330.0
58-59	37454.0
60-61	66839.0
62-63	70701.0
64-65	70907.0
66-67	92807.0
68-69	88158.0
70-71	91915.0
72-73	87159.0
74-75	84922.0
76-77	82332.0
78-79	87035.0
80-81	89515.0
82-83	86082.0
84-85	99039.0
86-87	104839.0
88-89	114606.0
90-91	115049.0
92-93	114258.0
94-95	128688.0
96-97	326868.0
98-99	637363.0
100-101	6287145.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.27942969999089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.55753760822815	66.11837712899393
2	8.988975144387547	13.893257454913257
3	2.681994064786029	6.2178891535627425
4	1.1784812417998802	3.6428943311372723
5	0.5755047615390837	2.2237339880684814
6	0.3258540266044343	1.5109088004861806
7	0.19727406546872	1.0671659091814942
8	0.12717173160970813	0.786220711420684
9	0.08529850955425955	0.5932638155351162
>10	0.27605558399762464	3.1578715457788764
>50	0.0036089290445249257	0.1849879078067958
>100	0.0018433042793046441	0.27815379412748753
>500	2.0051442002329818E-4	0.11258687897348209
>1k	2.0051428081793775E-4	0.2126885800141832
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0433861888430924E-5	0.0
2	0.0	0.0	0.0	2.0867723776861848E-5	0.0
3	0.0	0.0	0.0	2.0867723776861848E-5	0.0
4	0.0	1.0433861888430924E-5	0.0	4.1735447553723695E-5	0.0
5	0.0	1.0433861888430924E-5	0.0	4.1735447553723695E-5	1.0433861888430924E-5
6	0.0	1.0433861888430924E-5	0.0	4.1735447553723695E-5	1.0433861888430924E-5
7	0.0	1.0433861888430924E-5	0.0	6.260317133058554E-5	1.0433861888430924E-5
8	0.0	3.130158566529277E-5	0.0	6.260317133058554E-5	1.0433861888430924E-5
9	0.0	5.216930944215462E-5	0.0	7.303703321901646E-5	1.0433861888430924E-5
10-11	1.0433861888430924E-5	5.216930944215462E-5	0.0	1.35640204549602E-4	1.0433861888430924E-5
12-13	2.0867723776861848E-5	5.738624038637008E-5	0.0	1.773756521033257E-4	1.0433861888430924E-5
14-15	2.0867723776861848E-5	1.0433861888430924E-4	0.0	1.8259258304754116E-4	1.0433861888430924E-5
16-17	2.0867723776861848E-5	1.0433861888430924E-4	0.0	2.191110996570494E-4	1.0433861888430924E-5
18-19	2.0867723776861848E-5	1.095555498285247E-4	0.0	3.025819947644968E-4	1.0433861888430924E-5
20-21	2.0867723776861848E-5	1.1477248077274016E-4	0.0	3.964867517603751E-4	1.0433861888430924E-5
22-23	2.0867723776861848E-5	1.2520634266117108E-4	0.0	5.582116110310544E-4	1.0433861888430924E-5
24-25	2.0867723776861848E-5	1.3042327360538654E-4	0.0	0.0011842433243369097	1.0433861888430924E-5
26-27	2.0867723776861848E-5	1.35640204549602E-4	0.0	0.002801491917043703	1.0433861888430924E-5
28-29	2.0867723776861848E-5	1.35640204549602E-4	0.0	0.0077158408664946675	1.0433861888430924E-5
30-31	2.0867723776861848E-5	1.773756521033257E-4	0.0	0.008936602707441087	1.0433861888430924E-5
32-33	2.0867723776861848E-5	1.8780951399175662E-4	0.0	0.01146159728444137	1.0433861888430924E-5
34-35	2.0867723776861848E-5	2.0867723776861848E-4	0.0	0.01394485641388793	1.0433861888430924E-5
36-37	2.0867723776861848E-5	2.451957543781267E-4	0.0	0.019626094212138567	1.0433861888430924E-5
38-39	2.0867723776861848E-5	2.869312019318504E-4	0.0	0.08564113838024101	1.0433861888430924E-5
40-41	2.0867723776861848E-5	3.3910051137400505E-4	0.0	0.1563253357434163	1.0433861888430924E-5
42-43	2.0867723776861848E-5	3.547513042066514E-4	0.0	0.1737655358899286	1.0433861888430924E-5
44-45	2.0867723776861848E-5	3.9126982081615964E-4	0.0	0.19818598963980116	1.0433861888430924E-5
46-47	2.0867723776861848E-5	4.4343913025831423E-4	0.0	0.22720255955152757	1.0433861888430924E-5
48-49	2.0867723776861848E-5	4.5908992309096064E-4	0.0	0.2560939231205928	1.0433861888430924E-5
50-51	2.0867723776861848E-5	5.060423015888999E-4	0.0	0.2845574983522324	1.0433861888430924E-5
52-53	2.0867723776861848E-5	5.112592325331153E-4	0.0	0.651641627311009	1.0433861888430924E-5
54-55	2.0867723776861848E-5	5.321269563099771E-4	0.0	1.1566353257801216	1.0433861888430924E-5
56-57	2.0867723776861848E-5	5.42560818198408E-4	0.0	1.704919117224334	1.0433861888430924E-5
58-59	2.0867723776861848E-5	5.529946800868389E-4	0.0	2.313834080102218	1.0433861888430924E-5
60-61	2.0867723776861848E-5	6.2081478236164E-4	0.0	2.485105923000812	1.0433861888430924E-5
62-63	2.0867723776861848E-5	6.364655751942863E-4	0.0	2.4905471819756286	1.0433861888430924E-5
64-65	2.0867723776861848E-5	6.990687465248718E-4	0.0	2.495007657932933	1.0433861888430924E-5
66-67	2.0867723776861848E-5	6.990687465248718E-4	0.0	2.499379446064186	1.0433861888430924E-5
68-69	2.0867723776861848E-5	7.042856774690874E-4	0.0	2.5045389907680145	1.0433861888430924E-5
70-71	2.0867723776861848E-5	7.199364703017338E-4	0.0	2.5094168212008565	1.0433861888430924E-5
72-73	2.0867723776861848E-5	7.303703321901646E-4	0.0	2.513470376544512	1.0433861888430924E-5
74-75	2.0867723776861848E-5	7.408041940785955E-4	0.0	2.516939635622415	1.0433861888430924E-5
76-77	2.0867723776861848E-5	7.721057797438883E-4	0.0	2.520450630147872	1.0433861888430924E-5
78-79	2.0867723776861848E-5	7.825396416323193E-4	0.0	2.5226521750063307	1.0433861888430924E-5
80-81	2.0867723776861848E-5	7.825396416323193E-4	0.0	2.523346026821911	1.0433861888430924E-5
82-83	2.0867723776861848E-5	7.877565725765347E-4	0.0	2.523956407742385	1.0433861888430924E-5
84-85	2.0867723776861848E-5	8.034073654091811E-4	0.0	2.524191169634874	1.0433861888430924E-5
86-87	2.0867723776861848E-5	8.24275089186043E-4	0.0	2.524373762217922	1.0433861888430924E-5
88	2.0867723776861848E-5	8.660105367397666E-4	0.0	2.5243894130107547	1.0433861888430924E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	6060	0.0	51.020138	94
GGTATCA	15720	0.0	49.834743	1
TATCTCG	7985	0.0	38.061287	94
GTATCAA	28480	0.0	37.765526	1
ATCAACG	34565	0.0	30.456032	3
TCAACGC	35210	0.0	29.934675	4
TATCAAC	36165	0.0	29.537855	2
CAACGCA	35940	0.0	29.278694	5
AACGCAG	36055	0.0	29.260984	6
ACGCAGA	40335	0.0	26.102638	7
CGCAGAG	40790	0.0	25.82204	8
CGCTATC	12545	0.0	25.327532	94
GCAGAGT	46020	0.0	22.74699	9
TATGCCG	2775	0.0	21.966953	86-87
TACATGG	29910	0.0	21.855467	2
GTACATG	31625	0.0	21.392426	1
ACATGGG	30130	0.0	21.166496	3
CTATCTC	14230	0.0	21.034048	94
GTGGTAT	7150	0.0	20.852636	1
TCGTATG	2990	0.0	20.802988	84-85
>>END_MODULE