FastQCFastQC Report
Fri 27 May 2016
SRR2139408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5229382
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69700.053.7204361
GTATCAA134300.039.7972831
ATCAACG168300.031.3009973
TATCAAC173400.030.7254582
TCAACGC173250.030.4066834
CAACGCA173300.030.3734155
AACGCAG184700.028.6136286
ACGCAGA202400.025.964547
CGCAGAG212500.024.7304618
GTGGTAT33350.024.4404431
TGGTATC34450.022.7974452
GCAGAGT240900.021.7797159
TACATGG176250.020.4013082
ACATGGG177550.019.987393
GAGTACT137800.019.79221512-13
GTACATG189450.019.5624541
CAGAGTA232500.019.19803610-11
GTACTAG13150.019.047151
AGAGTAC222500.018.5536810-11
CATGGGG136700.017.9175174
Produced by FastQC (version 0.11.5)