##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5229382
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.797696936272775	32.0	25.0	33.0	18.0	34.0
2	32.17165164067188	33.0	32.0	33.0	32.0	34.0
3	32.71044685586175	33.0	33.0	34.0	31.0	34.0
4	32.9944207556457	33.0	33.0	34.0	33.0	34.0
5	33.2721818754109	34.0	33.0	34.0	33.0	34.0
6	36.94491739176828	38.0	38.0	38.0	35.0	38.0
7	37.06707542114919	38.0	38.0	38.0	36.0	38.0
8	37.01535879383071	38.0	38.0	38.0	35.0	38.0
9	37.24318973064121	38.0	38.0	38.0	36.0	38.0
10-11	37.34647774823105	38.0	38.0	38.0	37.0	38.0
12-13	37.438616264789985	38.0	38.0	38.0	37.0	38.0
14-15	37.41751491858885	38.0	38.0	38.0	37.0	38.0
16-17	37.43929846777306	38.0	38.0	38.0	37.0	38.0
18-19	37.454055469652054	38.0	38.0	38.0	37.0	38.0
20-21	37.466019402870614	38.0	38.0	38.0	37.0	38.0
22-23	37.47620875117693	38.0	38.0	38.0	37.0	38.0
24-25	37.49642876537867	38.0	38.0	38.0	37.0	38.0
26-27	37.46344233826552	38.0	38.0	38.0	37.0	38.0
28-29	37.44428512543657	38.0	38.0	38.0	37.0	38.0
30-31	37.45127112649084	38.0	38.0	38.0	37.0	38.0
32-33	37.42925476060241	38.0	38.0	38.0	37.0	38.0
34-35	37.40955369444182	38.0	38.0	38.0	37.0	38.0
36-37	37.38337036378768	38.0	38.0	38.0	37.0	38.0
38-39	37.38406139773722	38.0	38.0	38.0	37.0	38.0
40-41	37.36855396185254	38.0	38.0	38.0	37.0	38.0
42-43	37.33213108384078	38.0	38.0	38.0	37.0	38.0
44-45	37.29631619475465	38.0	38.0	38.0	37.0	38.0
46-47	37.264387345526465	38.0	38.0	38.0	37.0	38.0
48-49	37.20924580496476	38.0	38.0	38.0	37.0	38.0
50-51	37.14130253899772	38.0	38.0	38.0	37.0	38.0
52-53	37.13705880283671	38.0	38.0	38.0	37.0	38.0
54-55	37.08641396505499	38.0	38.0	38.0	36.5	38.0
56-57	37.06067535463728	38.0	38.0	38.0	36.0	38.0
58-59	37.070712361051676	38.0	38.0	38.0	36.0	38.0
60-61	37.08590227655422	38.0	38.0	38.0	36.0	38.0
62-63	37.100928629268154	38.0	38.0	38.0	36.0	38.0
64-65	37.10850499200998	38.0	38.0	38.0	36.0	38.0
66-67	37.11163439189638	38.0	38.0	38.0	36.0	38.0
68-69	37.10735411627888	38.0	38.0	38.0	36.0	38.0
70-71	37.1070421202931	38.0	38.0	38.0	36.0	38.0
72-73	37.11102994750243	38.0	38.0	38.0	36.0	38.0
74-75	37.098202296681606	38.0	38.0	38.0	36.0	38.0
76-77	37.08828613651177	38.0	38.0	38.0	36.0	38.0
78-79	37.06509720744015	38.0	38.0	38.0	36.0	38.0
80-81	37.05938747007107	38.0	38.0	38.0	36.0	38.0
82-83	37.005749768046904	38.0	38.0	38.0	36.0	38.0
84-85	36.98924187802395	38.0	38.0	38.0	36.0	38.0
86-87	36.985545866114734	38.0	38.0	38.0	36.0	38.0
88-89	36.963172752888674	38.0	38.0	38.0	36.0	38.0
90-91	36.96321172486276	38.0	38.0	38.0	36.0	38.0
92-93	36.96309261303137	38.0	38.0	38.0	35.5	38.0
94-95	36.96837705242794	38.0	38.0	38.0	35.0	38.0
96-97	36.975226152022536	38.0	38.0	38.0	35.0	38.0
98-99	36.96822109791161	38.0	38.0	38.0	35.0	38.0
100	35.429986544370074	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	51.0
22	178.0
23	701.0
24	1783.0
25	3816.0
26	6963.0
27	12146.0
28	19969.0
29	29649.0
30	39978.0
31	50928.0
32	65357.0
33	86518.0
34	125736.0
35	221273.0
36	635339.0
37	3928987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96480578393393	18.968206950649236	13.97801881751993	25.088968447896903
2	14.740311103427278	19.994044604648955	42.3583709832637	22.90727330866007
3	18.081505615768748	26.343533518874697	30.280748279624632	25.29421258573193
4	12.49524322376908	17.832011507287092	38.85843489727849	30.814310371665332
5	13.602486871297604	37.42736713439561	35.36169283483211	13.608453159474676
6	30.197009895241923	37.64733576548816	18.58877396984959	13.566880369420325
7	26.79702878848782	32.628310572836334	23.052934362033604	17.521726276642248
8	25.003757614188444	35.647730458398335	20.929681557017634	18.418830370395582
9	26.187759853841236	16.593031451900053	21.151332987339615	36.0678757069191
10-11	24.631218373413912	26.443774809336933	28.527701743724208	20.39730507352494
12-13	25.554778748234497	23.897020718700603	28.614633622099134	21.93356691096577
14-15	22.72074405732838	24.995668704256065	26.706520961750357	25.577066276665196
16-17	21.984672376200475	28.091311745823884	27.31888777679657	22.605128101179066
18-19	21.969861830709632	27.06003309760121	29.34492068087587	21.625184390813292
20-21	23.099420516614707	26.431034057437376	28.93625797499595	21.53328745095197
22-23	22.801287264984545	26.05559381389161	28.92056734360654	22.222551577517308
24-25	22.376412120412326	26.27256631413562	29.081999209159974	22.26902235629208
26-27	22.27402844209292	26.53656451117723	28.96575337880166	22.22365366792819
28-29	22.19758308532873	26.641171745797713	29.090817762184436	22.070427406689117
30-31	22.629168909431876	26.423831862526313	28.776082321774393	22.17091690626742
32-33	22.457396744719247	26.609748759199537	28.746847913348628	22.18600658273259
34-35	22.432377628387496	26.636103818088046	28.77819469457153	22.153323858952927
36-37	22.44652940406926	26.43301467628557	28.881832026492255	22.238623893152916
38-39	22.28417365843985	26.67052587272008	28.930854109373982	22.11444635946609
40-41	22.600407961403693	26.46188861211006	28.874754909469306	22.062948517016938
42-43	22.5783042676453	26.53912874793901	28.513784462882192	22.368782521533497
44-45	22.580781750863505	26.913381209154387	28.27846830504684	22.22736873493527
46-47	22.645492131308615	26.60564610785685	28.038485104409887	22.710376656424646
48-49	22.645372649522585	26.543667101996093	28.18361625692429	22.627343991557034
50-51	22.379078542998155	26.838035699512826	28.03003738720871	22.75284837028031
52-53	22.60379861161362	26.923647819980225	27.720445456264052	22.752108112142103
54-55	22.779882341829623	26.698693861104587	27.752087653767994	22.769336143297796
56-57	22.737065576678184	26.850173825935897	27.754662019567633	22.658098577818294
58-59	22.65600176681211	26.822109605813893	28.033588045925267	22.488300581448726
60-61	22.668654154768184	26.8639236869448	27.823556471876397	22.643865686410617
62-63	22.560291263013653	26.843070729091735	27.821072312492117	22.7755656954025
64-65	22.472511255785346	26.86581296947066	27.851051173401096	22.810624601342898
66-67	22.61819470301004	27.023827234705667	27.46788338461036	22.890094677673932
68-69	22.706676574458495	26.895225101412745	27.74217518851198	22.655923135616778
70-71	22.728320946762828	26.4168898807942	27.83605395540208	23.018735217040888
72-73	22.741816016203142	26.52087592468741	27.77835809793061	22.958949961178842
74-75	22.460555665466426	26.897653015432287	27.75879738203345	22.882993937067837
76-77	22.692986401854608	26.9145934973415	27.450607245424035	22.941812855379858
78-79	22.872310233673403	26.932273303091264	27.332616850979637	22.862799612255692
80-81	22.70185789434242	26.852191376455607	27.415889500085544	23.030061229116424
82-83	23.028830245191315	26.753367953316047	27.546002788520806	22.67179901297183
84-85	23.195799782338085	26.754407136113567	27.193883895269494	22.85590918627885
86-87	22.660814516886273	27.061311347958355	27.399671698386758	22.878202436768614
88-89	22.956995494274242	26.82952200206935	27.36521707589526	22.848265427761145
90-91	22.9694582116528	26.828155844453068	27.3946847429867	22.80770120090743
92-93	23.180310333111006	26.910172029749695	27.2774372978619	22.6320803392774
94-95	22.778861486716224	27.122967982067735	27.23622893739728	22.861941593818756
96-97	22.81752134362301	27.15296418496564	27.48756508717643	22.541949384234922
98-99	24.043825136428342	28.985021313689668	27.58229257877998	19.38886097110201
100	23.09959241716841	29.955025342721243	21.238902366921803	25.70647987318855
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	954.0
1	959.0
2	1390.0
3	2008.5
4	2786.0
5	4066.5
6	4967.5
7	5923.5
8	6991.0
9	7441.0
10	7408.5
11	7478.0
12	8464.5
13	10771.0
14	15150.5
15	20069.5
16	23568.0
17	25461.0
18	25057.5
19	22270.0
20	18784.0
21	16068.0
22	15505.0
23	17196.0
24	20726.0
25	27307.5
26	38291.5
27	50527.0
28	58435.0
29	66585.0
30	74402.5
31	81173.5
32	93181.0
33	106433.0
34	120042.0
35	134256.0
36	152904.5
37	168215.5
38	169917.5
39	170403.5
40	177445.5
41	183062.5
42	185381.5
43	190978.0
44	198990.5
45	207660.5
46	211163.5
47	213350.0
48	219086.5
49	223066.0
50	225875.5
51	225906.5
52	220498.0
53	212502.5
54	202797.0
55	189590.5
56	175213.0
57	161924.0
58	148271.0
59	132481.0
60	110370.0
61	86174.0
62	64964.0
63	47779.5
64	35287.5
65	25083.0
66	17739.5
67	12971.0
68	9736.5
69	7239.5
70	5294.5
71	3815.5
72	2828.0
73	1936.0
74	1256.0
75	808.5
76	463.5
77	278.0
78	181.5
79	118.5
80	70.5
81	43.0
82	27.5
83	15.0
84	8.0
85	4.5
86	3.5
87	2.5
88	2.0
89	2.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009752586443292917
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.2429915594180084E-4
22-23	0.0
24-25	4.3032222528229143E-4
26-27	0.0031178402051424085
28-29	0.006773523139952755
30-31	0.007575343301438042
32-33	0.00257198856847051
34-35	9.142594056120515E-4
36-37	1.1594308199514083E-4
38-39	4.666020811619325E-4
40-41	1.1744952484326605E-4
42-43	1.872838596404071E-4
44-45	1.4909403012593973E-4
46-47	3.0108819294694886E-5
48-49	8.309936302311429E-4
50-51	7.055877024039679E-4
52-53	0.0
54-55	0.007896037446334218
56-57	0.06512917670833139
58-59	0.0792976487924785
60-61	0.04834774024176643
62-63	0.00994369543881399
64-65	7.601219192122879E-5
66-67	1.0963265386668889E-5
68-69	6.755266976456566E-4
70-71	0.0037258466119299144
72-73	0.013903140549899728
74-75	0.012236472106379688
76-77	0.01755109825824377
78-79	0.007505368261266065
80-81	0.00878933610821864
82-83	0.04289016591979098
84-85	0.02885757343428109
86-87	0.009778033768755825
88-89	0.0023238160157399803
90-91	1.243806156293199E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	36.0
20-21	179.0
22-23	440.0
24-25	541.0
26-27	1263.0
28-29	3510.0
30-31	10383.0
32-33	13626.0
34-35	17824.0
36-37	29222.0
38-39	35117.0
40-41	35443.0
42-43	39838.0
44-45	47357.0
46-47	49105.0
48-49	45524.0
50-51	39972.0
52-53	37653.0
54-55	39892.0
56-57	40834.0
58-59	42602.0
60-61	42404.0
62-63	41546.0
64-65	43104.0
66-67	45513.0
68-69	46045.0
70-71	46084.0
72-73	43271.0
74-75	43774.0
76-77	45462.0
78-79	46334.0
80-81	46582.0
82-83	43380.0
84-85	43212.0
86-87	44440.0
88-89	46069.0
90-91	47280.0
92-93	46039.0
94-95	53294.0
96-97	160720.0
98-99	351902.0
100-101	3372566.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.0952540942243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.23131086356052	72.43371884410494
2	7.807789032692196	12.81964849105927
3	2.1201092965517736	5.22152734223835
4	0.840582090925909	2.760312013664674
5	0.37531228572881536	1.5405678730795604
6	0.20626613573721692	1.0160082494628349
7	0.1289876055038512	0.7412489174190959
8	0.075302307620568	0.49455696623935785
9	0.05363312562215323	0.3962722568236309
>10	0.15696785498613194	1.8848044694317265
>50	0.0019723112109773845	0.11025361878066989
>100	0.00140798072981654	0.22514514364551821
>500	2.394060869547137E-4	0.14186987056283765
>1k	1.1970304297121762E-4	0.21406594348743613
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.912271851626062E-5	0.0
9	0.0	0.0	0.0	1.912271851626062E-5	0.0
10-11	0.0	0.0	0.0	1.912271851626062E-5	0.0
12-13	0.0	0.0	0.0	1.912271851626062E-5	0.0
14-15	0.0	0.0	0.0	2.868407777439093E-5	0.0
16-17	0.0	0.0	0.0	3.824543703252124E-5	0.0
18-19	0.0	0.0	0.0	3.824543703252124E-5	0.0
20-21	0.0	0.0	0.0	3.824543703252124E-5	0.0
22-23	0.0	0.0	0.0	4.780679629065155E-5	0.0
24-25	0.0	0.0	0.0	8.605223332317279E-5	0.0
26-27	0.0	0.0	0.0	2.1034990367886685E-4	0.0
28-29	0.0	0.0	0.0	4.589452443902549E-4	0.0
30-31	0.0	0.0	0.0	0.0016827992294309348	0.0
32-33	0.0	0.0	0.0	0.004541645647611898	0.0
34-35	0.0	0.0	0.0	0.008146278087927023	0.0
36-37	0.0	0.0	0.0	0.014571511509390594	0.0
38-39	0.0	0.0	0.0	0.03203055351473654	0.0
40-41	0.0	0.0	0.0	0.05074213358289756	0.0
42-43	0.0	0.0	1.912271851626062E-5	0.06132655828164782	0.0
44-45	0.0	0.0	1.912271851626062E-5	0.07445430454306073	0.0
46-47	0.0	0.0	1.912271851626062E-5	0.0883565209043822	0.0
48-49	0.0	0.0	1.912271851626062E-5	0.10200058056573415	0.0
50-51	0.0	0.0	1.912271851626062E-5	0.11550121983821415	0.0
52-53	0.0	0.0	1.912271851626062E-5	0.15474103823358093	0.0
54-55	0.0	0.0	1.912271851626062E-5	0.2038194953055638	0.0
56-57	0.0	0.0	1.912271851626062E-5	0.2740285563380147	0.0
58-59	0.0	0.0	1.912271851626062E-5	0.3184888768883206	0.0
60-61	0.0	0.0	1.912271851626062E-5	0.33395915616797545	0.0
62-63	0.0	0.0	1.912271851626062E-5	0.34755540903303683	0.0
64-65	0.0	1.912271851626062E-5	1.912271851626062E-5	0.3554148463432199	0.0
66-67	0.0	1.912271851626062E-5	1.912271851626062E-5	0.3624806908349782	0.0
68-69	0.0	1.912271851626062E-5	1.912271851626062E-5	0.3702540759118381	0.0
70-71	0.0	1.912271851626062E-5	1.912271851626062E-5	0.37669843205181797	0.0
72-73	0.0	1.912271851626062E-5	1.912271851626062E-5	0.380589905269877	0.0
74-75	0.0	1.912271851626062E-5	1.912271851626062E-5	0.3839746264472551	0.0
76-77	0.0	1.912271851626062E-5	1.912271851626062E-5	0.3872446113135357	0.0
78-79	0.0	1.912271851626062E-5	1.912271851626062E-5	0.38951065345771263	0.0
80-81	0.0	1.912271851626062E-5	1.912271851626062E-5	0.3901417031687492	0.0
82-83	0.0	1.912271851626062E-5	1.912271851626062E-5	0.3906197711316557	0.0
84-85	0.0	1.912271851626062E-5	1.912271851626062E-5	0.39076319152052763	0.0
86-87	0.0	1.912271851626062E-5	1.912271851626062E-5	0.39088748919088334	0.0
88	0.0	1.912271851626062E-5	1.912271851626062E-5	0.39088748919088334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6970	0.0	53.720436	1
GTATCAA	13430	0.0	39.797283	1
ATCAACG	16830	0.0	31.300997	3
TATCAAC	17340	0.0	30.725458	2
TCAACGC	17325	0.0	30.406683	4
CAACGCA	17330	0.0	30.373415	5
AACGCAG	18470	0.0	28.613628	6
ACGCAGA	20240	0.0	25.96454	7
CGCAGAG	21250	0.0	24.730461	8
GTGGTAT	3335	0.0	24.440443	1
TGGTATC	3445	0.0	22.797445	2
GCAGAGT	24090	0.0	21.779715	9
TACATGG	17625	0.0	20.401308	2
ACATGGG	17755	0.0	19.98739	3
GAGTACT	13780	0.0	19.792215	12-13
GTACATG	18945	0.0	19.562454	1
CAGAGTA	23250	0.0	19.198036	10-11
GTACTAG	1315	0.0	19.04715	1
AGAGTAC	22250	0.0	18.55368	10-11
CATGGGG	13670	0.0	17.917517	4
>>END_MODULE