FastQCFastQC Report
Fri 27 May 2016
SRR2139407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5665386
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75000.052.9871671
GTATCAA140600.039.0280531
ATCAACG173250.031.1011143
TCAACGC175750.030.756274
CAACGCA176950.030.4992435
TATCAAC179000.030.416062
AACGCAG188250.028.8734176
ACGCAGA205550.026.2764687
CGCAGAG220350.024.492138
TGGTATC35100.022.8395082
GCAGAGT256050.021.1107799
GTGGTAT40100.021.0607641
TACATGG183850.020.2165812
GTACATG192450.020.092821
ACATGGG182600.019.8836693
GAGTACT141450.019.28898412-13
CAGAGTA247800.018.829610-11
GTACTTT149150.018.13526214-15
AGAGTAC233950.018.11204710-11
AGTACTT144000.017.8459912-13
Produced by FastQC (version 0.11.5)