##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139407_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5665386
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.17499337203149	32.0	18.0	33.0	18.0	34.0
2	32.050382092235196	33.0	32.0	33.0	30.0	34.0
3	32.65705831870944	33.0	33.0	33.0	31.0	34.0
4	32.93435610565635	33.0	33.0	34.0	31.0	34.0
5	33.26323396146353	34.0	33.0	34.0	33.0	34.0
6	36.83122385659159	38.0	37.0	38.0	34.0	38.0
7	36.93624459127763	38.0	38.0	38.0	35.0	38.0
8	36.87376853051143	38.0	38.0	38.0	35.0	38.0
9	37.16689454169583	38.0	38.0	38.0	36.0	38.0
10-11	37.28839332395003	38.0	38.0	38.0	37.0	38.0
12-13	37.39316526711507	38.0	38.0	38.0	37.0	38.0
14-15	37.36685426200439	38.0	38.0	38.0	37.0	38.0
16-17	37.38696348315896	38.0	38.0	38.0	37.0	38.0
18-19	37.41231974308546	38.0	38.0	38.0	37.0	38.0
20-21	37.42778899281869	38.0	38.0	38.0	37.0	38.0
22-23	37.44126039434197	38.0	38.0	38.0	37.0	38.0
24-25	37.460103155957924	38.0	38.0	38.0	37.0	38.0
26-27	37.430886397494604	38.0	38.0	38.0	37.0	38.0
28-29	37.40937377870054	38.0	38.0	38.0	37.0	38.0
30-31	37.41719254005572	38.0	38.0	38.0	37.0	38.0
32-33	37.397259675029275	38.0	38.0	38.0	37.0	38.0
34-35	37.379008075469926	38.0	38.0	38.0	37.0	38.0
36-37	37.35505506542119	38.0	38.0	38.0	37.0	38.0
38-39	37.353885913173166	38.0	38.0	38.0	37.0	38.0
40-41	37.34088387067035	38.0	38.0	38.0	37.0	38.0
42-43	37.307363743111566	38.0	38.0	38.0	37.0	38.0
44-45	37.27486971744513	38.0	38.0	38.0	37.0	38.0
46-47	37.24452288727886	38.0	38.0	38.0	37.0	38.0
48-49	37.1959437836166	38.0	38.0	38.0	37.0	38.0
50-51	37.12132277646005	38.0	38.0	38.0	36.5	38.0
52-53	37.11100565591954	38.0	38.0	38.0	36.0	38.0
54-55	37.04984994546675	38.0	38.0	38.0	36.0	38.0
56-57	37.01879845983579	38.0	38.0	38.0	36.0	38.0
58-59	37.02517902201794	38.0	38.0	38.0	36.0	38.0
60-61	37.03779852376319	38.0	38.0	38.0	36.0	38.0
62-63	37.041032330855366	38.0	38.0	38.0	36.0	38.0
64-65	37.04487058475742	38.0	38.0	38.0	36.0	38.0
66-67	37.040397644785024	38.0	38.0	38.0	36.0	38.0
68-69	37.0278672121198	38.0	38.0	38.0	36.0	38.0
70-71	37.020858965340935	38.0	38.0	38.0	36.0	38.0
72-73	37.02387790866582	38.0	38.0	38.0	36.0	38.0
74-75	37.008981544022305	38.0	38.0	38.0	36.0	38.0
76-77	36.99635513912591	38.0	38.0	38.0	36.0	38.0
78-79	36.966710985825344	38.0	38.0	38.0	36.0	38.0
80-81	36.95983764277382	38.0	38.0	38.0	36.0	38.0
82-83	36.89925497108828	38.0	38.0	38.0	35.0	38.0
84-85	36.87509357314734	38.0	38.0	38.0	35.0	38.0
86-87	36.866245462602166	38.0	38.0	38.0	35.0	38.0
88-89	36.84113899571683	38.0	38.0	38.0	35.0	38.0
90-91	36.83606985173893	38.0	38.0	38.0	35.0	38.0
92-93	36.833937820414164	38.0	38.0	38.0	35.0	38.0
94-95	36.83756923339348	38.0	38.0	38.0	35.0	38.0
96-97	36.844259540916894	38.0	38.0	38.0	35.0	38.0
98-99	36.829512602429375	38.0	38.0	38.0	35.0	38.0
100	35.18041537876747	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	12.0
21	47.0
22	264.0
23	899.0
24	2196.0
25	4689.0
26	8621.0
27	14325.0
28	22577.0
29	33346.0
30	44174.0
31	57574.0
32	74098.0
33	100279.0
34	148277.0
35	264266.0
36	763763.0
37	4125976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7523889810862	18.943139973163348	14.251403170057609	25.053067875692847
2	14.744920844990911	20.010270494606047	42.44439889979665	22.800409760606385
3	18.27453592747255	26.065673195083267	30.14302997183246	25.51676090561173
4	12.62461198583821	18.088458579874345	38.64608695682871	30.64084247745873
5	13.827654461673045	37.383419240983756	35.244835921153474	13.544090376189724
6	30.360967460999127	37.734904559018574	18.556705580166998	13.3474223998153
7	27.143516787735205	32.488854245765424	22.788950302768427	17.578678663730944
8	24.981263412590067	35.99823560124588	20.72105943001942	18.29944155614463
9	25.959502141601646	17.07348802005724	21.1752208940397	35.79178894430141
10-11	24.688008901776506	26.68393645199109	28.465130178243815	20.16292446798859
12-13	25.51555180882644	24.018160457204505	28.589420032456747	21.876867701512307
14-15	22.746261243276276	25.217187319628355	26.46422503250441	25.572326404590967
16-17	22.103816050662743	28.39593807023917	27.118787669542726	22.381458209555362
18-19	22.10790226826557	27.401663364155592	29.138297019832365	21.35213734774647
20-21	23.14933070282051	26.64906950786528	28.73989121686842	21.46170857244579
22-23	22.795973063614078	26.485145500014035	28.683175138229117	22.03570629814277
24-25	22.512554776817403	26.67002319855481	28.682203454909345	22.135218569718447
26-27	22.398612778284562	26.923238885854456	28.758154240134242	21.91999409572674
28-29	22.310648883246163	26.910401372199967	28.74024861144855	22.038701133105324
30-31	22.588365833742845	26.761541996826644	28.58408607465896	22.066006094771552
32-33	22.461561432631015	26.835476130255415	28.54192924696399	22.16103319014958
34-35	22.466109280707915	27.0682586957616	28.494384105205377	21.971247918325105
36-37	22.47413917912692	26.819345110154337	28.46696260922441	22.239553101494337
38-39	22.423927360350106	26.89631441945282	28.62306217687644	22.056696043320628
40-41	22.55327755413965	26.695653749232655	28.71207421467055	22.038994481957143
42-43	22.617236153575163	26.70563986687724	28.376431699121962	22.300692280425636
44-45	22.645182590854972	27.121591170875675	28.060399313292766	22.172826924976587
46-47	22.65893235187783	27.07215434657442	27.85648480942203	22.41242849212572
48-49	22.62632275704211	26.96105766445667	27.87930116008081	22.5333184184204
50-51	22.537243913383417	27.29398687809913	27.686436568620955	22.482332639896498
52-53	22.651007139124413	27.26031438961029	27.54505796319454	22.543620508070756
54-55	22.780768709925777	27.061264298908426	27.55388052366186	22.604086467503933
56-57	22.82178575722773	27.05355637831941	27.717202977061074	22.40745488739179
58-59	22.730482369249884	26.996053937416104	27.86343760309684	22.41002609023717
60-61	22.73306944402901	27.272216201511995	27.530574658306158	22.46413969615284
62-63	22.764289896320868	27.25288982556005	27.55810967298841	22.42471060513067
64-65	22.54285340833993	27.15158548136747	27.625182197299182	22.680378912993422
66-67	22.75639838808279	27.22773695307724	27.407014085561364	22.608850573278605
68-69	22.825948259583306	27.198348335116762	27.596268647321708	22.37943475797822
70-71	22.85788115638864	26.912497467542096	27.541451748709832	22.688169627359432
72-73	22.699927222611493	26.962509025350396	27.610661800202518	22.72690195183559
74-75	22.415229763104996	27.152405282367337	27.74451858123845	22.687846373289226
76-77	22.820176388331348	27.111273646343182	27.403763944107517	22.66478602121795
78-79	22.754739368978377	27.12011969324178	27.341982016486927	22.78315892129292
80-81	22.63386395601858	27.166082017357546	27.36745688415507	22.832597142468803
82-83	23.03438063138313	26.977052283553938	27.505893196882976	22.482673888179956
84-85	22.994180060593052	27.00174724164326	27.300552931169452	22.703519766594233
86-87	22.735260284942317	27.15679107256175	27.502257426923617	22.60569121557232
88-89	22.872695938260712	26.928311878363964	27.476600618668478	22.722391564706843
90-91	22.935438451523723	27.083137963904008	27.32566090074129	22.655762683830975
92-93	23.00057117974065	27.08112448890724	27.36338907519779	22.55491525615432
94-95	22.68954688329244	27.209301447982337	27.378270647810943	22.722881020914283
96-97	22.728473436292987	27.22566518170888	27.570984660599056	22.474876721399074
98-99	24.003156553035033	29.106401513193987	27.57879783160499	19.311644102165992
100	22.96066508303423	30.149272086295547	21.297451743042682	25.592611087627542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1246.0
1	1203.0
2	1360.0
3	1680.5
4	2287.5
5	3370.5
6	4179.5
7	5053.0
8	6055.0
9	6520.0
10	6510.0
11	6500.0
12	7282.0
13	9414.5
14	13786.5
15	18907.0
16	22652.5
17	24645.0
18	24298.5
19	22251.5
20	19797.0
21	17723.5
22	17395.0
23	19709.5
24	24064.5
25	30500.0
26	39982.0
27	51082.0
28	60383.0
29	69540.5
30	78917.5
31	89064.0
32	102636.0
33	115395.0
34	127992.5
35	142120.0
36	158746.0
37	172479.0
38	177663.5
39	182097.5
40	189686.0
41	196513.0
42	202309.0
43	209415.5
44	220177.5
45	232507.5
46	235279.5
47	237691.5
48	249014.0
49	255273.0
50	257621.5
51	256392.0
52	245162.0
53	232688.0
54	219223.0
55	202154.5
56	184780.0
57	169316.5
58	152827.5
59	129875.5
60	103307.0
61	79227.5
62	59453.5
63	43418.5
64	31952.5
65	23365.5
66	16634.5
67	12327.5
68	9318.0
69	6836.5
70	5018.5
71	3549.0
72	2407.5
73	1669.0
74	1228.0
75	854.0
76	509.0
77	307.5
78	204.0
79	138.5
80	84.0
81	49.5
82	32.5
83	24.5
84	14.0
85	8.0
86	7.5
87	5.5
88	4.5
89	5.5
90	5.0
91	3.0
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0108553944956266
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.1473324960747991E-4
22-23	0.0
24-25	5.384309504233082E-4
26-27	0.003310450323647279
28-29	0.007117665878925325
30-31	0.00788458732008092
32-33	0.0027984165921307817
34-35	9.942759003767506E-4
36-37	1.5146058790585422E-4
38-39	5.643754397425301E-4
40-41	1.1716915711212278E-4
42-43	1.0884330047716903E-4
44-45	1.5534684655953338E-4
46-47	3.68727824478181E-5
48-49	8.647599175409575E-4
50-51	5.249257722150227E-4
52-53	0.0
54-55	0.007768888407097359
56-57	0.06471766272841388
58-59	0.07888217763103501
60-61	0.04718316964165722
62-63	0.00981602029481828
64-65	7.915700167921684E-5
66-67	1.9961819028743722E-5
68-69	3.7263032292345207E-4
70-71	0.003202001789868175
72-73	0.013029076692941288
74-75	0.011219320746718244
76-77	0.01640751633440837
78-79	0.0065271074780511515
80-81	0.00735994665902624
82-83	0.04251773849084723
84-85	0.027643259072928045
86-87	0.008967506710830358
88-89	0.002435992093212573
90-91	1.1190610451157293E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	36.0
20-21	205.0
22-23	440.0
24-25	577.0
26-27	1462.0
28-29	3610.0
30-31	10092.0
32-33	12856.0
34-35	17630.0
36-37	28629.0
38-39	33842.0
40-41	34475.0
42-43	38635.0
44-45	46543.0
46-47	47886.0
48-49	44230.0
50-51	39243.0
52-53	37014.0
54-55	39913.0
56-57	40815.0
58-59	42240.0
60-61	41295.0
62-63	39947.0
64-65	42979.0
66-67	44795.0
68-69	45722.0
70-71	45866.0
72-73	42926.0
74-75	42913.0
76-77	45114.0
78-79	47272.0
80-81	46611.0
82-83	43671.0
84-85	43882.0
86-87	44779.0
88-89	47105.0
90-91	47977.0
92-93	47138.0
94-95	55632.0
96-97	181557.0
98-99	391068.0
100-101	3756764.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.26517759308854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.29111945279465	72.63284621676745
2	7.810761465981303	12.851073582724087
3	2.084951254196533	5.145566555982314
4	0.8148752871071905	2.6814344084036814
5	0.37864704890159623	1.5574733361494346
6	0.19002628104491728	0.9379527454508563
7	0.12619025921472868	0.7266744859372273
8	0.07809773313981587	0.513977910909167
9	0.05031261447961295	0.37250785488041616
>10	0.1708372293289829	2.1446233670846047
>50	0.002876945876868292	0.15680952148693816
>100	0.001152489528662287	0.16700432981364755
>500	7.596920438411342E-5	0.033122986435158576
>1k	7.596920076049482E-5	0.07893269797507049
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.7651047960368456E-5
10-11	0.0	0.0	0.0	0.0	1.7651047960368456E-5
12-13	0.0	0.0	0.0	1.7651047960368456E-5	1.7651047960368456E-5
14-15	0.0	1.7651047960368456E-5	0.0	1.7651047960368456E-5	1.7651047960368456E-5
16-17	0.0	1.7651047960368456E-5	0.0	1.7651047960368456E-5	1.7651047960368456E-5
18-19	0.0	1.7651047960368456E-5	0.0	3.530209592073691E-5	1.7651047960368456E-5
20-21	0.0	1.7651047960368456E-5	0.0	3.530209592073691E-5	1.7651047960368456E-5
22-23	0.0	1.7651047960368456E-5	0.0	3.530209592073691E-5	3.530209592073691E-5
24-25	0.0	1.7651047960368456E-5	0.0	9.70807637820265E-5	5.2953143881105367E-5
26-27	0.0	1.7651047960368456E-5	0.0	1.323828597027634E-4	5.2953143881105367E-5
28-29	0.0	1.7651047960368456E-5	0.0	4.942293428903167E-4	5.2953143881105367E-5
30-31	0.0	1.7651047960368456E-5	0.0	0.0013767817409087394	5.2953143881105367E-5
32-33	0.0	1.7651047960368456E-5	0.0	0.0035655116879944283	7.060419184147382E-5
34-35	0.0	1.7651047960368456E-5	0.0	0.006469109077475039	7.060419184147382E-5
36-37	0.0	1.7651047960368456E-5	0.0	0.011270194122695257	7.060419184147382E-5
38-39	0.0	1.7651047960368456E-5	0.0	0.023811263698537047	7.060419184147382E-5
40-41	0.0	1.7651047960368456E-5	0.0	0.03753495348772352	8.825523980184227E-5
42-43	0.0	1.7651047960368456E-5	0.0	0.04582212050511651	8.825523980184227E-5
44-45	0.0	1.7651047960368456E-5	0.0	0.05676577024054495	9.70807637820265E-5
46-47	0.0	3.530209592073691E-5	0.0	0.067930058075478	1.2355733572257918E-4
48-49	0.0	3.530209592073691E-5	0.0	0.07883840571498571	1.4120838368294764E-4
50-51	0.0	3.530209592073691E-5	0.0	0.08941138344324641	1.5003390766313188E-4
52-53	0.0	3.530209592073691E-5	0.0	0.11313439190198162	1.9416152756405303E-4
54-55	0.0	3.530209592073691E-5	0.0	0.14299996505092505	2.2946362348478993E-4
56-57	0.0	3.530209592073691E-5	0.0	0.18278542715359553	2.2946362348478993E-4
58-59	0.0	3.530209592073691E-5	0.0	0.21444258167051639	2.2946362348478993E-4
60-61	0.0	3.530209592073691E-5	0.0	0.22490082758703467	2.2946362348478993E-4
62-63	0.0	3.530209592073691E-5	0.0	0.23636518323729397	2.2946362348478993E-4
64-65	0.0	3.530209592073691E-5	0.0	0.24276368812292753	2.3828914746497413E-4
66-67	0.0	3.530209592073691E-5	0.0	0.24853558080596802	2.4711467144515836E-4
68-69	0.0	3.530209592073691E-5	0.0	0.25454576263647344	2.4711467144515836E-4
70-71	0.0	3.530209592073691E-5	0.0	0.25927624348985223	2.647657194055268E-4
72-73	0.0	3.530209592073691E-5	0.0	0.2633183334727766	3.177188632866322E-4
74-75	0.0	3.530209592073691E-5	0.0	0.26681324096892955	3.177188632866322E-4
76-77	0.0	3.530209592073691E-5	0.0	0.2698845233140337	3.177188632866322E-4
78-79	0.0	3.530209592073691E-5	0.0	0.27179083649375346	3.177188632866322E-4
80-81	0.0	3.530209592073691E-5	0.0	0.272293891360624	3.177188632866322E-4
82-83	0.0	3.530209592073691E-5	0.0	0.2727528186075936	3.353699112470006E-4
84-85	0.0	3.530209592073691E-5	0.0	0.27298228223107834	3.353699112470006E-4
86-87	0.0	3.530209592073691E-5	0.0	0.27303523537495944	3.353699112470006E-4
88	0.0	3.530209592073691E-5	0.0	0.2730440608989396	3.353699112470006E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7500	0.0	52.987167	1
GTATCAA	14060	0.0	39.028053	1
ATCAACG	17325	0.0	31.101114	3
TCAACGC	17575	0.0	30.75627	4
CAACGCA	17695	0.0	30.499243	5
TATCAAC	17900	0.0	30.41606	2
AACGCAG	18825	0.0	28.873417	6
ACGCAGA	20555	0.0	26.276468	7
CGCAGAG	22035	0.0	24.49213	8
TGGTATC	3510	0.0	22.839508	2
GCAGAGT	25605	0.0	21.110779	9
GTGGTAT	4010	0.0	21.060764	1
TACATGG	18385	0.0	20.216581	2
GTACATG	19245	0.0	20.09282	1
ACATGGG	18260	0.0	19.883669	3
GAGTACT	14145	0.0	19.288984	12-13
CAGAGTA	24780	0.0	18.8296	10-11
GTACTTT	14915	0.0	18.135262	14-15
AGAGTAC	23395	0.0	18.112047	10-11
AGTACTT	14400	0.0	17.84599	12-13
>>END_MODULE