FastQCFastQC Report
Fri 27 May 2016
SRR2139406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6703836
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99050.052.9725531
GTATCAA183050.038.6672821
ATCAACG220150.031.7603113
TATCAAC227700.030.9916732
CAACGCA227450.030.8343455
TCAACGC227450.030.7222884
AACGCAG240550.029.3494016
ACGCAGA261850.026.7673247
CGCAGAG272900.025.6834898
GCAGAGT313550.022.3808659
GTGGTAT45200.022.276571
TGGTATC45000.021.525872
GAGTACT185000.020.88356212-13
TACATGG221550.020.8063742
ACATGGG219950.020.4524633
GTACATG228400.020.4055791
CAGAGTA304100.020.13593710-11
GTACTTT206850.018.54479414-15
AGAGTAC291050.018.49194510-11
CATGGGG166800.017.9542244
Produced by FastQC (version 0.11.5)