##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139406_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6703836
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.626718792046823	32.0	25.0	33.0	18.0	34.0
2	32.113687745344606	33.0	32.0	33.0	31.0	34.0
3	32.688865897077434	33.0	33.0	33.0	31.0	34.0
4	32.982719893505745	33.0	33.0	34.0	33.0	34.0
5	33.26846047546509	34.0	33.0	34.0	33.0	34.0
6	36.93740210828547	38.0	38.0	38.0	35.0	38.0
7	37.06756460032734	38.0	38.0	38.0	36.0	38.0
8	37.0056695897692	38.0	38.0	38.0	35.0	38.0
9	37.24338900891967	38.0	38.0	38.0	36.0	38.0
10-11	37.345290293497634	38.0	38.0	38.0	37.0	38.0
12-13	37.43924030957798	38.0	38.0	38.0	37.0	38.0
14-15	37.42460376417323	38.0	38.0	38.0	37.0	38.0
16-17	37.44556377870819	38.0	38.0	38.0	37.0	38.0
18-19	37.45785651677637	38.0	38.0	38.0	37.0	38.0
20-21	37.47042882388706	38.0	38.0	38.0	37.0	38.0
22-23	37.47993720848626	38.0	38.0	38.0	37.0	38.0
24-25	37.49752534263093	38.0	38.0	38.0	37.0	38.0
26-27	37.46722012941895	38.0	38.0	38.0	37.0	38.0
28-29	37.446554881033094	38.0	38.0	38.0	37.0	38.0
30-31	37.45301978879818	38.0	38.0	38.0	37.0	38.0
32-33	37.432896374760816	38.0	38.0	38.0	37.0	38.0
34-35	37.41520827938014	38.0	38.0	38.0	37.0	38.0
36-37	37.39034461814707	38.0	38.0	38.0	37.0	38.0
38-39	37.39150075776146	38.0	38.0	38.0	37.0	38.0
40-41	37.37867791013333	38.0	38.0	38.0	37.0	38.0
42-43	37.344311908289185	38.0	38.0	38.0	37.0	38.0
44-45	37.310332676863716	38.0	38.0	38.0	37.0	38.0
46-47	37.27870047267936	38.0	38.0	38.0	37.0	38.0
48-49	37.227165909087056	38.0	38.0	38.0	37.0	38.0
50-51	37.16263163904614	38.0	38.0	38.0	37.0	38.0
52-53	37.16140020013903	38.0	38.0	38.0	37.0	38.0
54-55	37.11028688943058	38.0	38.0	38.0	36.5	38.0
56-57	37.08697612220452	38.0	38.0	38.0	36.0	38.0
58-59	37.09760935798844	38.0	38.0	38.0	36.0	38.0
60-61	37.11517189473558	38.0	38.0	38.0	36.0	38.0
62-63	37.126631993380215	38.0	38.0	38.0	36.0	38.0
64-65	37.13501284854988	38.0	38.0	38.0	36.0	38.0
66-67	37.13660493446362	38.0	38.0	38.0	36.0	38.0
68-69	37.13210086188862	38.0	38.0	38.0	36.0	38.0
70-71	37.13030296102848	38.0	38.0	38.0	36.0	38.0
72-73	37.13259488667997	38.0	38.0	38.0	36.0	38.0
74-75	37.12274907649196	38.0	38.0	38.0	36.0	38.0
76-77	37.11649324185254	38.0	38.0	38.0	36.0	38.0
78-79	37.095533450821826	38.0	38.0	38.0	36.0	38.0
80-81	37.09254505133441	38.0	38.0	38.0	36.0	38.0
82-83	37.043533933560354	38.0	38.0	38.0	36.0	38.0
84-85	37.02476145157719	38.0	38.0	38.0	36.0	38.0
86-87	37.019510121581696	38.0	38.0	38.0	36.0	38.0
88-89	36.99698289903592	38.0	38.0	38.0	36.0	38.0
90-91	36.995656277297435	38.0	38.0	38.0	36.0	38.0
92-93	36.99600699582808	38.0	38.0	38.0	36.0	38.0
94-95	36.999783725072135	38.0	38.0	38.0	36.0	38.0
96-97	37.01174177753953	38.0	38.0	38.0	36.0	38.0
98-99	37.00020733352835	38.0	38.0	38.0	35.0	38.0
100	35.482164134592736	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	5.0
21	64.0
22	288.0
23	912.0
24	2313.0
25	4834.0
26	8908.0
27	15368.0
28	24906.0
29	37070.0
30	49671.0
31	63411.0
32	81679.0
33	108748.0
34	159852.0
35	280209.0
36	817731.0
37	5047865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68294093113256	19.139131685202322	14.173974423001996	25.003952960663117
2	14.687506433546277	20.10206849142179	42.444383577502265	22.766041497529667
3	18.00045526173373	26.33362450990746	30.339599596410178	25.326320631948633
4	12.475797438958828	18.147848485553645	38.86470373081919	30.511650344668332
5	13.620574846998046	37.43149444586651	35.30603970622193	13.641891000913505
6	30.1051517370055	37.80854722579729	18.63094502908484	13.455356008112371
7	26.834024579360232	32.61380499164956	23.076698176984042	17.475472252006167
8	24.908932736421356	35.81917875079283	20.94599271223222	18.325895800553592
9	26.0945076818705	16.750737935713225	21.23151580677093	35.92323857564534
10-11	24.5601548128564	26.54487669447761	28.58469389764308	20.31027459502291
12-13	25.530994493302046	24.075924590040685	28.614699106601055	21.778381810056214
14-15	22.664292503575563	25.210566010266362	26.64620673894767	25.478934747210403
16-17	21.944391241074513	28.241644037831477	27.29740852849026	22.51655619260376
18-19	21.87064987866648	27.26959609393786	29.39813861794948	21.461615409446175
20-21	22.92250809007541	26.47174408727309	29.09852465158061	21.507223171070887
22-23	22.750977830861274	26.267260516935554	28.998503838040673	21.9832578141625
24-25	22.345022651521045	26.479718733698927	29.132236352190965	22.043022262589062
26-27	22.229918912446482	26.747798982843218	29.017504437098395	22.004777667611904
28-29	22.0900117129595	26.766254436766662	29.12644488318909	22.017288967084745
30-31	22.44148248383814	26.5630901070793	28.931015760993123	22.064411648089436
32-33	22.30925461308979	26.78272599193306	28.817593499491934	22.090425895485218
34-35	22.305593866326507	26.786066157624056	28.921521416819846	21.986818559229597
36-37	22.391300661832446	26.63817377199279	28.864342246311693	22.106183319863074
38-39	22.288845131175222	26.79435917165258	28.97876445315387	21.938031244018322
40-41	22.436120558663557	26.548309632392673	29.004858119838406	22.01071168910536
42-43	22.48054789783588	26.678083126014577	28.579695889805677	22.26167308634386
44-45	22.48020541889975	27.013228413078565	28.346374757773525	22.160191410248164
46-47	22.465017957773007	26.82222950823263	28.16625590935473	22.546496624639634
48-49	22.62772016129631	26.76027859582422	28.134285954845943	22.477715288033526
50-51	22.38630189010311	27.016579987436977	27.9803613315466	22.616756790913314
52-53	22.564613212454486	27.103394395361402	27.722366938536965	22.609625453647155
54-55	22.67984776831887	26.985551920835636	27.69548318332352	22.639117127521974
56-57	22.765339733304206	27.02424326603875	27.73021954704733	22.48019745360971
58-59	22.6352767245163	26.941015157065213	27.976813947272028	22.446894171146464
60-61	22.661870084513005	27.150947371854894	27.6858901660468	22.501292377585298
62-63	22.66280904345491	27.096216787794358	27.667500293727652	22.57347387502308
64-65	22.517852256996647	27.036560894075706	27.7224607263891	22.72312612253854
66-67	22.684336124184696	27.046191118128	27.549501470911835	22.71997128677547
68-69	22.628409760640896	26.99463116938911	27.78073174169097	22.59622732827902
70-71	22.67911654737071	26.71055090948781	27.804928272478797	22.80540427066268
72-73	22.717019019367367	26.817226972152504	27.74338119112143	22.722372817358703
74-75	22.54408601251964	27.129072209234213	27.68056353569647	22.64627824254968
76-77	22.70443655080216	27.05881254347467	27.53014146332397	22.706609442399202
78-79	22.705688128688493	26.99529547537053	27.55303305812788	22.7459833378131
80-81	22.598206245055685	27.079944109376346	27.57248458501872	22.749365060549245
82-83	22.916490581089818	26.882910876231485	27.72507258432831	22.47552595835039
84-85	23.01245016172518	26.953563027141897	27.332032518011793	22.70195429312113
86-87	22.603463518643714	27.19183619438443	27.528789375895308	22.675910911076546
88-89	22.86986045251994	26.916096156641327	27.45956387458626	22.754479516252477
90-91	22.839310813677233	26.932005687975963	27.517801499689078	22.71088199865773
92-93	22.932503509018485	27.04268268250268	27.4390683690301	22.585745439448736
94-95	22.605391558157123	27.175510096752824	27.483474576396848	22.7356237686932
96-97	22.79695024197979	27.180623293968903	27.539095128088007	22.4833313359633
98-99	23.964438010225518	29.119326416017266	27.59636546720542	19.319870106551797
100	22.933417636311624	29.997717942254297	21.37236616328238	25.6964982581517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1136.0
1	1132.5
2	1703.0
3	2445.5
4	3338.5
5	4976.0
6	6164.0
7	7225.5
8	8457.0
9	9082.5
10	9103.0
11	9374.5
12	10918.5
13	14075.5
14	19945.0
15	26806.0
16	31821.5
17	34494.0
18	33918.0
19	30297.5
20	25861.0
21	22242.5
22	21129.0
23	23277.0
24	27899.5
25	35347.5
26	46501.0
27	58852.0
28	68696.0
29	80950.5
30	94495.5
31	107122.5
32	124253.0
33	142235.5
34	159405.5
35	176969.0
36	197847.5
37	216906.0
38	223447.0
39	225239.0
40	230958.0
41	236991.5
42	242538.5
43	251644.5
44	262362.0
45	273732.0
46	278765.5
47	282120.0
48	288651.0
49	289018.5
50	287437.5
51	284399.5
52	275783.0
53	265459.0
54	254425.5
55	237608.0
56	217709.5
57	199008.0
58	179072.5
59	156210.0
60	127991.0
61	100298.5
62	78423.5
63	59431.0
64	43589.5
65	31096.5
66	22381.0
67	16769.5
68	12536.5
69	9043.0
70	6576.5
71	4870.0
72	3515.0
73	2404.0
74	1630.0
75	1046.5
76	622.0
77	377.0
78	236.5
79	170.0
80	116.0
81	63.5
82	31.0
83	19.0
84	13.0
85	9.0
86	6.5
87	5.0
88	3.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010292614556800016
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.966816297016181E-5
22-23	7.458805761658934E-6
24-25	4.550200044696391E-4
26-27	0.0033496266434525353
28-29	0.007306072107125673
30-31	0.007664850883911948
32-33	0.0025827559937906053
34-35	9.00783974812879E-4
36-37	1.7332331172921053E-4
38-39	5.306489571421369E-4
40-41	7.631467869345302E-5
42-43	1.306346569749071E-4
44-45	1.3946646947140967E-4
46-47	3.911600335834359E-5
48-49	9.16196602521847E-4
50-51	4.860717339883987E-4
52-53	0.0
54-55	0.008122790679895396
56-57	0.06546487331203077
58-59	0.0791299481257397
60-61	0.04727882261424939
62-63	0.00980115076250939
64-65	5.925916394293207E-5
66-67	8.54560180947991E-6
68-69	7.422012982826756E-4
70-71	0.003670107705020629
72-73	0.012951343536808537
74-75	0.011662511452506245
76-77	0.01694029689957555
78-79	0.007020779420807467
80-81	0.008635329221926587
82-83	0.042418622989040915
84-85	0.027969750747318237
86-87	0.00946397501510596
88-89	0.002388980036440546
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	50.0
20-21	204.0
22-23	479.0
24-25	611.0
26-27	1693.0
28-29	4672.0
30-31	13380.0
32-33	16883.0
34-35	22461.0
36-37	36520.0
38-39	44245.0
40-41	44190.0
42-43	50492.0
44-45	60743.0
46-47	62190.0
48-49	56956.0
50-51	52078.0
52-53	48908.0
54-55	53317.0
56-57	54945.0
58-59	53873.0
60-61	53468.0
62-63	51762.0
64-65	54476.0
66-67	57232.0
68-69	57835.0
70-71	57772.0
72-73	54248.0
74-75	54450.0
76-77	57015.0
78-79	57877.0
80-81	57684.0
82-83	54353.0
84-85	54513.0
86-87	55515.0
88-89	57814.0
90-91	58611.0
92-93	57151.0
94-95	66634.0
96-97	202784.0
98-99	448034.0
100-101	4355718.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.34197834802158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.18603542883801	70.04698570673547
2	8.158397759954287	13.109236323695905
3	2.396464310008405	5.776100511195053
4	0.97990910896858	3.1491134566313113
5	0.49882347471136984	2.0038232402372884
6	0.25974240425663486	1.2520931171309777
7	0.15839600368410905	0.8908093808881288
8	0.09968268496347446	0.6406963293606498
9	0.06580573064695104	0.47582663281317733
>10	0.1932441544745415	2.2195272430763033
>50	0.002150184904548514	0.116178916025394
>100	0.0011438181964333238	0.18423944647169252
>500	1.7078033177625018E-4	0.08276404305057938
>1k	3.415606086873624E-5	0.052605652688095424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4916832691014518E-5	0.0	0.0	0.0
3	0.0	1.4916832691014518E-5	0.0	1.4916832691014518E-5	0.0
4	0.0	2.9833665382029037E-5	0.0	1.4916832691014518E-5	0.0
5	0.0	2.9833665382029037E-5	0.0	1.4916832691014518E-5	0.0
6	0.0	2.9833665382029037E-5	0.0	1.4916832691014518E-5	0.0
7	0.0	2.9833665382029037E-5	0.0	1.4916832691014518E-5	0.0
8	0.0	2.9833665382029037E-5	0.0	2.9833665382029037E-5	0.0
9	0.0	7.458416345507259E-5	0.0	2.9833665382029037E-5	0.0
10-11	0.0	7.458416345507259E-5	0.0	1.267930778736234E-4	0.0
12-13	0.0	7.458416345507259E-5	0.0	1.4916832691014517E-4	0.0
14-15	0.0	8.95009961460871E-5	0.0	1.640851596011597E-4	0.0
16-17	0.0	8.95009961460871E-5	0.0	1.9391882498318873E-4	0.0
18-19	0.0	8.95009961460871E-5	0.0	2.2375249036521777E-4	0.0
20-21	0.0	8.95009961460871E-5	0.0	2.2375249036521777E-4	0.0
22-23	0.0	8.95009961460871E-5	0.0	2.685029884382613E-4	0.0
24-25	0.0	1.0441782883710163E-4	0.0	3.580039845843484E-4	0.0
26-27	0.0	1.0441782883710163E-4	0.0	4.102128990028992E-4	0.0
28-29	0.0	1.0441782883710163E-4	0.0	5.668396422585517E-4	0.0
30-31	0.0	1.3425149421913065E-4	0.0	0.0015140585181379735	0.0
32-33	0.0	1.3425149421913065E-4	0.0	0.0033861210208602954	0.0
34-35	0.0	1.5662674325565243E-4	0.0	0.005847398414877691	0.0
36-37	0.0	1.790019922921742E-4	0.0	0.008890432283844653	0.0
38-39	0.0	1.9391882498318873E-4	0.0	0.014290325717991907	0.0
40-41	0.0	2.0883565767420325E-4	0.0	0.02059268752994554	0.0
42-43	0.0	2.2375249036521777E-4	0.0	0.02690996617459019	0.0
44-45	0.0	2.685029884382613E-4	0.0	0.03304824282694266	0.0
46-47	0.0	2.9833665382029035E-4	0.0	0.039559440296570506	0.0
48-49	0.0	3.0579507016579763E-4	0.0	0.04645847541616471	0.0
50-51	0.0	4.176713153484065E-4	0.0	0.052962214469447044	0.0
52-53	0.0	4.32588148039421E-4	0.0	0.05910794953814502	0.0
54-55	0.0	4.4004656438492826E-4	0.0	0.0657608569183375	0.0
56-57	0.0	4.4750498073043555E-4	0.0	0.07292093661002447	0.0
58-59	0.0	4.5496339707594283E-4	0.0	0.0794172172469613	0.0
60-61	0.0	4.698802297669573E-4	0.0	0.08616708403964536	0.0
62-63	0.0	4.773386461124646E-4	0.0	0.0922755270266158	0.0
64-65	0.0	5.370059768765226E-4	0.0	0.09889860074142626	0.0
66-67	0.0	5.370059768765226E-4	0.0	0.1058274695264025	0.0
68-69	0.0	5.519228095675372E-4	0.0	0.1121074560893196	0.0
70-71	7.458416345507259E-6	5.519228095675372E-4	0.0	0.11729105544944715	0.0
72-73	1.4916832691014518E-5	5.519228095675372E-4	0.0	0.1212514745289115	0.0
74-75	1.4916832691014518E-5	5.519228095675372E-4	0.0	0.124331800479606	0.0
76-77	1.4916832691014518E-5	5.742980586040589E-4	0.0	0.12746433534471904	0.0
78-79	1.4916832691014518E-5	5.966733076405807E-4	0.0	0.12990323748969992	0.0
80-81	1.4916832691014518E-5	5.966733076405807E-4	0.0	0.13067891278963267	0.0
82-83	1.4916832691014518E-5	6.04131723986088E-4	0.0	0.13123083559920024	0.0
84-85	1.4916832691014518E-5	6.115901403315953E-4	0.0	0.13136508709341935	0.0
86-87	1.4916832691014518E-5	6.115901403315953E-4	0.0	0.13140983759149238	0.0
88	1.4916832691014518E-5	6.265069730226097E-4	0.0	0.1314172960078379	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9905	0.0	52.972553	1
GTATCAA	18305	0.0	38.667282	1
ATCAACG	22015	0.0	31.760311	3
TATCAAC	22770	0.0	30.991673	2
CAACGCA	22745	0.0	30.834345	5
TCAACGC	22745	0.0	30.722288	4
AACGCAG	24055	0.0	29.349401	6
ACGCAGA	26185	0.0	26.767324	7
CGCAGAG	27290	0.0	25.683489	8
GCAGAGT	31355	0.0	22.380865	9
GTGGTAT	4520	0.0	22.27657	1
TGGTATC	4500	0.0	21.52587	2
GAGTACT	18500	0.0	20.883562	12-13
TACATGG	22155	0.0	20.806374	2
ACATGGG	21995	0.0	20.452463	3
GTACATG	22840	0.0	20.405579	1
CAGAGTA	30410	0.0	20.135937	10-11
GTACTTT	20685	0.0	18.544794	14-15
AGAGTAC	29105	0.0	18.491945	10-11
CATGGGG	16680	0.0	17.954224	4
>>END_MODULE