FastQCFastQC Report
Fri 27 May 2016
SRR2139405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5384395
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77500.048.949831
CTTATAC61650.047.096361
GTATCAA141650.038.5415461
TATACAC81550.038.458583
ATCAACG169650.031.577573
CAACGCA174550.030.691125
TTATACA98850.030.6590882
TATCAAC177450.030.622592
TCAACGC174850.030.5172634
AACGCAG181300.029.6185866
ACACATC108650.027.5397826
ACATCTC109100.027.0765618
ACGCAGA201250.026.6403627
TACACAT118100.026.376855
CGCAGAG205100.026.1402878
ATACACA135100.024.469484
CACATCT122050.024.3078067
CATCTCC126550.023.3764619
GCAGAGT238550.022.4926059
TGGTATC35850.022.1096742
Produced by FastQC (version 0.11.5)