##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139405_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5384395
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.311356800531907	32.0	25.0	33.0	18.0	34.0
2	32.10084605605644	33.0	32.0	33.0	31.0	34.0
3	32.689219494483595	33.0	33.0	33.0	31.0	34.0
4	32.97062418340408	33.0	33.0	34.0	33.0	34.0
5	33.2821184181324	34.0	33.0	34.0	33.0	34.0
6	36.893237958953605	38.0	37.0	38.0	35.0	38.0
7	37.00601943208104	38.0	38.0	38.0	35.0	38.0
8	36.949515405166224	38.0	38.0	38.0	35.0	38.0
9	37.21598081121463	38.0	38.0	38.0	36.0	38.0
10-11	37.32221001616709	38.0	38.0	38.0	37.0	38.0
12-13	37.42700841970175	38.0	38.0	38.0	37.0	38.0
14-15	37.40178590166583	38.0	38.0	38.0	37.0	38.0
16-17	37.41856011678192	38.0	38.0	38.0	37.0	38.0
18-19	37.443168359676434	38.0	38.0	38.0	37.0	38.0
20-21	37.45649542937487	38.0	38.0	38.0	37.0	38.0
22-23	37.46762362064761	38.0	38.0	38.0	37.0	38.0
24-25	37.481004290225655	38.0	38.0	38.0	37.0	38.0
26-27	37.44904803022037	38.0	38.0	38.0	37.0	38.0
28-29	37.43303347354349	38.0	38.0	38.0	37.0	38.0
30-31	37.43406014569692	38.0	38.0	38.0	37.0	38.0
32-33	37.415102094388715	38.0	38.0	38.0	37.0	38.0
34-35	37.39219086855687	38.0	38.0	38.0	37.0	38.0
36-37	37.36407556759987	38.0	38.0	38.0	37.0	38.0
38-39	37.34010249885151	38.0	38.0	38.0	37.0	38.0
40-41	37.349654547597865	38.0	38.0	38.0	37.0	38.0
42-43	37.3198957837324	38.0	38.0	38.0	37.0	38.0
44-45	37.28422824030578	38.0	38.0	38.0	37.0	38.0
46-47	37.24938778973651	38.0	38.0	38.0	37.0	38.0
48-49	37.195384912228064	38.0	38.0	38.0	37.0	38.0
50-51	37.124688685505724	38.0	38.0	38.0	36.5	38.0
52-53	37.11851658224717	38.0	38.0	38.0	36.0	38.0
54-55	37.05575286886594	38.0	38.0	38.0	36.0	38.0
56-57	37.02651515086032	38.0	38.0	38.0	36.0	38.0
58-59	37.02845974417822	38.0	38.0	38.0	36.0	38.0
60-61	37.031319707640066	38.0	38.0	38.0	36.0	38.0
62-63	37.040397719291036	38.0	38.0	38.0	36.0	38.0
64-65	37.04698069685433	38.0	38.0	38.0	36.0	38.0
66-67	37.04356161905625	38.0	38.0	38.0	36.0	38.0
68-69	37.03119052935453	38.0	38.0	38.0	36.0	38.0
70-71	37.029600887488556	38.0	38.0	38.0	36.0	38.0
72-73	37.033762483546894	38.0	38.0	38.0	36.0	38.0
74-75	37.02703415705021	38.0	38.0	38.0	36.0	38.0
76-77	37.021725425577685	38.0	38.0	38.0	36.0	38.0
78-79	37.002117089187934	38.0	38.0	38.0	36.0	38.0
80-81	36.99885316208829	38.0	38.0	38.0	36.0	38.0
82-83	36.94723753948382	38.0	38.0	38.0	36.0	38.0
84-85	36.927688979726625	38.0	38.0	38.0	35.5	38.0
86-87	36.925933198781614	38.0	38.0	38.0	35.0	38.0
88-89	36.90256945355236	38.0	38.0	38.0	35.0	38.0
90-91	36.902441141012744	38.0	38.0	38.0	35.0	38.0
92-93	36.901614883914604	38.0	38.0	38.0	35.0	38.0
94-95	36.90897059925925	38.0	38.0	38.0	35.0	38.0
96-97	36.92060063104756	38.0	38.0	38.0	35.0	38.0
98-99	36.906094313699924	38.0	38.0	38.0	35.0	38.0
100	35.26454018854571	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	13.0
21	63.0
22	250.0
23	821.0
24	1949.0
25	4207.0
26	7730.0
27	13461.0
28	21967.0
29	32091.0
30	43190.0
31	53869.0
32	68688.0
33	91632.0
34	134624.0
35	240400.0
36	703327.0
37	3966111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53179605136696	19.669600762945514	14.430980639421884	25.367622546265643
2	13.905064857005515	20.198319267794535	43.90660279161135	21.990013083588604
3	17.51563546136567	27.00115797596573	30.996592931982143	24.486613630686456
4	11.855463798625472	18.80985700343307	39.5690137889215	29.765665409019952
5	12.937070924402835	37.94220149153247	36.16649595729882	12.954231626765866
6	28.796810040868102	38.80497251780376	19.017642650659916	13.380574790668218
7	25.682384000430876	33.14548802604564	23.3235303130621	17.848597660461387
8	23.936393968124552	36.849506769098475	21.47639614107063	17.73770312170634
9	25.237542936578762	17.183341861063315	21.449986488732716	36.12912871362521
10-11	23.843226583488025	27.089115861670628	29.20983694546927	19.857820609372084
12-13	24.90315996504714	24.295570811576788	29.1268378341485	21.674431389227575
14-15	22.009919405987116	25.689720014969186	27.013703489435674	25.28665708960802
16-17	21.437078817583032	28.94978915922773	27.601011812840625	22.01212021034861
18-19	21.359363493948717	27.855961533282752	29.56396215359386	21.22071281917467
20-21	22.41227865308938	27.059750073198437	29.246407612154258	21.28156366155793
22-23	21.90673436168545	27.21829897692263	29.19554774497283	21.67941891641909
24-25	21.81132596259203	27.202339126540593	29.552173878317202	21.434161032550175
26-27	21.833864655553008	27.39553158028737	29.319512174958938	21.451091589200686
28-29	21.463400764031213	27.388684711124395	29.485431148358686	21.66248337648571
30-31	21.95676221992768	27.199757197096076	29.510105331478886	21.33337525149736
32-33	21.362282366401157	27.174490668365603	29.756354378245504	21.706872586987735
34-35	21.43925012696765	27.214976902867765	29.596875965105973	21.748897005058616
36-37	21.598518729739464	27.252399372673793	29.775548910433425	21.37353298715332
38-39	21.44378612699039	27.233463748307308	29.754039981911916	21.568710142790387
40-41	21.78938882614389	27.129010946245764	29.62375063827856	21.45784958933179
42-43	22.009606385620117	27.035295469640474	29.488433530433237	21.46666461430617
44-45	21.687916544252875	27.64383909699876	29.20068227856984	21.467562080178528
46-47	22.048808158726004	27.010518521617676	28.713739657711418	22.226933661944905
48-49	22.031469122077883	27.040288018478435	28.871750855374028	22.056492004069657
50-51	21.565779875656695	27.39051413750352	28.955206375734548	22.088499611105235
52-53	21.806107184557685	27.38861376499882	29.061426703141578	21.743852347301914
54-55	21.85888486837139	27.383320835618992	28.675420099344244	22.082374196665373
56-57	22.182831989036238	27.71176208998905	28.20933451351668	21.89607140745803
58-59	21.662309037925976	28.093693476512417	28.904674680439708	21.339322805121903
60-61	22.006765283538584	28.54514350765941	28.03219997859677	21.41589123020524
62-63	21.646621026018384	28.770896068880177	28.0221957986524	21.560287106449046
64-65	21.570309416646907	28.706501466658985	28.150457124252963	21.572731992441142
66-67	21.812219139657778	28.68676861282896	27.86545713881594	21.635555108697318
68-69	21.83274687736595	28.361737384834484	28.22984544887632	21.575670288923245
70-71	21.86230056479885	27.70424979028393	28.410305734607388	22.023143910309834
72-73	22.031196631114934	27.47098007133103	28.597332988152203	21.900490309401835
74-75	21.84360101353193	27.66840980099748	28.496401406461917	21.99158777900867
76-77	21.84745154671858	27.75549401752925	28.29357550467469	22.103478931077472
78-79	21.971372538851284	27.792060426283278	28.239139624981195	21.997427409884242
80-81	21.892601066619008	27.610663973450205	28.332803418057434	22.16393154187335
82-83	22.067273634127694	27.540602850145635	28.50932366499589	21.88279985073078
84-85	22.43559078311019	27.558755225814746	28.109403253373678	21.89625073770139
86-87	21.817251089620626	27.712734235971816	28.280721249871636	22.189293424535926
88-89	22.190446934955844	27.628247683152875	28.24444394404622	21.936861437845064
90-91	22.255936172619755	27.608863674528877	28.287147710900975	21.848052441950394
92-93	22.20701636738506	27.703505867913375	28.13164169772233	21.95783606697923
94-95	21.938344120897828	27.88776529157832	28.114725411905823	22.059165175618027
96-97	21.976493238548535	28.008963350477718	28.27458162525815	21.739961785715597
98-99	23.03582360501488	29.921537323978825	28.440849292974065	18.60178977803223
100	22.229925386597344	30.8071130210333	22.196475407181946	24.76648618518741
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	985.0
1	929.5
2	1230.5
3	1791.0
4	2469.0
5	3651.0
6	4579.5
7	5424.5
8	6417.0
9	6970.0
10	7043.0
11	7166.0
12	8161.5
13	10518.5
14	15006.5
15	20448.5
16	24748.5
17	27036.0
18	26620.0
19	24325.5
20	21662.0
21	19319.5
22	19246.0
23	22029.0
24	26931.5
25	34994.5
26	47797.0
27	61776.5
28	71556.5
29	81741.0
30	91397.5
31	100431.0
32	113636.5
33	127696.0
34	144351.5
35	160272.5
36	176275.0
37	190816.5
38	195882.0
39	198567.0
40	205652.5
41	209619.5
42	210443.5
43	214813.5
44	227388.5
45	247119.0
46	249084.5
47	241837.0
48	237964.5
49	227627.0
50	216783.0
51	206915.0
52	196966.0
53	185812.5
54	172125.0
55	157204.0
56	142786.0
57	128524.5
58	113205.5
59	99109.5
60	83525.5
61	64532.0
62	47422.5
63	34179.0
64	24576.5
65	17495.5
66	12562.5
67	9171.5
68	6517.0
69	4705.0
70	3515.0
71	2514.0
72	1797.5
73	1235.5
74	832.5
75	555.5
76	354.5
77	206.5
78	128.5
79	95.0
80	59.5
81	29.5
82	18.5
83	14.5
84	12.0
85	9.0
86	5.0
87	2.5
88	1.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010233275976223883
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.5717140421773176E-5
22-23	9.286519493286637E-6
24-25	4.364993310879932E-4
26-27	0.0033252008333064745
28-29	0.007405231847402794
30-31	0.008072935437442889
32-33	0.00240445013394092
34-35	9.905013721714527E-4
36-37	1.3131811306376975E-4
38-39	6.03813060608585E-4
40-41	1.8992816631857582E-4
42-43	1.2428875758472142E-4
44-45	2.024160963593152E-4
46-47	3.891963377792204E-5
48-49	8.936663703818931E-4
50-51	5.150897524082427E-4
52-53	0.0
54-55	0.008041506654271273
56-57	0.06417544401042724
58-59	0.07743741457150717
60-61	0.046858355995146324
62-63	0.009712274646018364
64-65	1.3692800957232115E-4
66-67	0.0
68-69	5.176011902239369E-4
70-71	0.0035739259478171836
72-73	0.01282074154415582
74-75	0.012042552291369777
76-77	0.016730277518000027
78-79	0.007492641278262877
80-81	0.008554109591650358
82-83	0.04226029330417039
84-85	0.027678616370958527
86-87	0.009493742884904269
88-89	0.0024203167893642854
90-91	1.2177449798463206E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	28.0
20-21	148.0
22-23	380.0
24-25	496.0
26-27	1314.0
28-29	3533.0
30-31	10464.0
32-33	13333.0
34-35	17693.0
36-37	28432.0
38-39	35013.0
40-41	34456.0
42-43	40100.0
44-45	47674.0
46-47	48526.0
48-49	44721.0
50-51	40776.0
52-53	38989.0
54-55	41796.0
56-57	43863.0
58-59	43169.0
60-61	44928.0
62-63	45720.0
64-65	51270.0
66-67	56505.0
68-69	57223.0
70-71	69458.0
72-73	46300.0
74-75	43696.0
76-77	45324.0
78-79	46087.0
80-81	46680.0
82-83	43862.0
84-85	43774.0
86-87	45028.0
88-89	45814.0
90-91	47215.0
92-93	46638.0
94-95	52428.0
96-97	158630.0
98-99	354015.0
100-101	3458896.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.67661357458752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.88352922265493	74.37472727909655
2	7.5895166589869705	12.701301053838948
3	1.9575821607767705	4.914115380234716
4	0.7541890022194467	2.524319268036815
5	0.33287470098381766	1.392691386148964
6	0.17241151783710112	0.8656087172317929
7	0.10177475048804474	0.5961316527766727
8	0.05834586768364526	0.39057476990707185
9	0.03523482252652437	0.26534975660290366
>10	0.11026773711105194	1.355662367878072
>50	0.002611711435844901	0.1517247346661973
>100	0.001376794262955549	0.1979051737182937
>500	2.1378977636152786E-4	0.12683445895724457
>1k	7.126325657786839E-5	0.1430540009057641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.857218870458055E-5	0.0
2	0.0	0.0	0.0	1.857218870458055E-5	0.0
3	0.0	0.0	0.0	3.71443774091611E-5	0.0
4	0.0	0.0	0.0	3.71443774091611E-5	0.0
5	0.0	0.0	0.0	3.71443774091611E-5	0.0
6	0.0	0.0	0.0	3.71443774091611E-5	0.0
7	0.0	0.0	0.0	5.571656611374165E-5	0.0
8	0.0	0.0	0.0	5.571656611374165E-5	0.0
9	0.0	0.0	0.0	5.571656611374165E-5	0.0
10-11	0.0	0.0	0.0	1.0214703787519302E-4	0.0
12-13	0.0	0.0	0.0	1.2071922657977359E-4	0.0
14-15	0.0	9.286094352290275E-6	0.0	1.485775096366444E-4	0.0
16-17	0.0	1.857218870458055E-5	0.0	1.578636039889347E-4	0.0
18-19	0.0	1.857218870458055E-5	0.0	1.6714969834122497E-4	0.0
20-21	0.0	1.857218870458055E-5	0.0	1.6714969834122497E-4	0.0
22-23	0.0	1.857218870458055E-5	0.0	2.69296736216418E-4	0.0
24-25	0.0	1.857218870458055E-5	0.0	3.250133023301597E-4	0.0
26-27	0.0	1.857218870458055E-5	0.0	4.0858815150077214E-4	0.0
28-29	0.0	1.857218870458055E-5	0.0	7.428875481832221E-4	0.0
30-31	0.0	1.857218870458055E-5	0.0	0.0015229194737756053	0.0
32-33	0.0	1.857218870458055E-5	0.0	0.0028415448718008243	0.0
34-35	0.0	1.857218870458055E-5	0.0	0.005144496271168813	0.0
36-37	0.0	2.7858283056870826E-5	0.0	0.008227479596129184	0.0
38-39	0.0	3.71443774091611E-5	0.0	0.012916957244035774	0.0
40-41	0.0	3.71443774091611E-5	0.0	0.01853504432717139	0.0
42-43	0.0	3.71443774091611E-5	0.0	0.024413142052171134	0.0
44-45	0.0	3.71443774091611E-5	0.0	0.03003122913530675	0.0
46-47	0.0	3.71443774091611E-5	0.0	0.03605790436994314	0.0
48-49	0.0	3.71443774091611E-5	0.0	0.04247459556737572	0.0
50-51	0.0	3.71443774091611E-5	0.0	0.049002719897035786	0.0
52-53	0.0	3.71443774091611E-5	0.0	0.05542869718882065	0.0
54-55	0.0	3.71443774091611E-5	0.0	0.06143680023475247	0.0
56-57	0.0	3.71443774091611E-5	0.0	0.06719417873317243	0.0
58-59	0.0	3.71443774091611E-5	0.0	0.07384302228941228	0.0
60-61	0.0	3.71443774091611E-5	0.0	0.07977683658052576	0.0
62-63	0.0	3.71443774091611E-5	0.0	0.08609138074008316	0.0
64-65	0.0	3.71443774091611E-5	0.0	0.09226663348435618	0.0
66-67	0.0	3.71443774091611E-5	0.0	0.09792186494490096	0.0
68-69	0.0	3.71443774091611E-5	0.0	0.1037071017263778	0.0
70-71	0.0	4.643047176145138E-5	0.0	0.10944590803609319	0.0
72-73	0.0	5.571656611374165E-5	0.0	0.11350393126804405	0.0
74-75	0.0	5.571656611374165E-5	0.0	0.11668906163087961	0.0
76-77	0.0	5.571656611374165E-5	0.0	0.11992062246547663	0.0
78-79	0.0	5.571656611374165E-5	0.0	0.12191713275121903	0.0
80-81	0.0	5.571656611374165E-5	0.0	0.12253930107282249	0.0
82-83	0.0	5.571656611374165E-5	0.0	0.12310575282831218	0.0
84-85	0.0	5.571656611374165E-5	0.0	0.12335647737582403	0.0
86-87	0.0	5.571656611374165E-5	0.0	0.12348648269675609	0.0
88	0.0	5.571656611374165E-5	0.0	0.12352362707416525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7750	0.0	48.94983	1
CTTATAC	6165	0.0	47.09636	1
GTATCAA	14165	0.0	38.541546	1
TATACAC	8155	0.0	38.45858	3
ATCAACG	16965	0.0	31.57757	3
CAACGCA	17455	0.0	30.69112	5
TTATACA	9885	0.0	30.659088	2
TATCAAC	17745	0.0	30.62259	2
TCAACGC	17485	0.0	30.517263	4
AACGCAG	18130	0.0	29.618586	6
ACACATC	10865	0.0	27.539782	6
ACATCTC	10910	0.0	27.076561	8
ACGCAGA	20125	0.0	26.640362	7
TACACAT	11810	0.0	26.37685	5
CGCAGAG	20510	0.0	26.140287	8
ATACACA	13510	0.0	24.46948	4
CACATCT	12205	0.0	24.307806	7
CATCTCC	12655	0.0	23.376461	9
GCAGAGT	23855	0.0	22.492605	9
TGGTATC	3585	0.0	22.109674	2
>>END_MODULE