FastQCFastQC Report
Fri 27 May 2016
SRR2139404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5302690
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74000.051.7115631
GTATCAA141200.040.3656921
ATCAACG173750.032.1641773
CAACGCA177800.031.4792965
TCAACGC178000.031.4439264
TATCAAC180400.031.2648332
AACGCAG185450.030.2723396
ACGCAGA203500.027.5455117
CGCAGAG206550.027.0976458
GCAGAGT240550.023.2499359
GTGGTAT36000.021.7075041
GTACTAG18300.020.6553961
GAGTACT148550.020.38253412-13
TGGTATC35450.020.3670082
CAGAGTA241150.019.47642310-11
TACATGG165250.019.4557952
AGAGTAC227150.019.396210-11
ACATGGG167750.018.8597153
GTACATG179600.018.4924741
GTACTTT163450.018.45995714-15
Produced by FastQC (version 0.11.5)