FastQCFastQC Report
Fri 27 May 2016
SRR2139403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5824671
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76750.052.574041
CTTATAC75250.046.8351361
TATACAC91000.040.7653243
GTATCAA152100.040.4317131
TTATACA99550.036.462722
ATCAACG187500.032.38943
TCAACGC192150.031.7593254
CAACGCA191700.031.7017885
TATCAAC196850.031.1760312
AACGCAG207450.029.4373326
ATACACA130300.028.955864
TACACAT127200.028.5666565
ACGCAGA223950.027.174257
ACACATC135750.026.1456576
CGCAGAG237350.025.6756448
ACATCTC140500.025.1115388
CACATCT146700.024.136557
GTGGTAT38250.023.3935571
GCAGAGT270700.022.5280159
CATCTCC160550.022.0018149
Produced by FastQC (version 0.11.5)