##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5824671
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.327142425726706	32.0	25.0	33.0	18.0	34.0
2	32.09194751085512	33.0	32.0	33.0	31.0	34.0
3	32.684353330857654	33.0	33.0	33.0	31.0	34.0
4	32.96560440924475	33.0	33.0	34.0	33.0	34.0
5	33.27675520213931	34.0	33.0	34.0	33.0	34.0
6	36.88848898075101	38.0	37.0	38.0	35.0	38.0
7	37.0084236517393	38.0	38.0	38.0	35.0	38.0
8	36.96053614015281	38.0	38.0	38.0	35.0	38.0
9	37.22497270661296	38.0	38.0	38.0	36.0	38.0
10-11	37.32618803705823	38.0	38.0	38.0	37.0	38.0
12-13	37.42824959555655	38.0	38.0	38.0	37.0	38.0
14-15	37.402065369872396	38.0	38.0	38.0	37.0	38.0
16-17	37.41935098136874	38.0	38.0	38.0	37.0	38.0
18-19	37.44392198288968	38.0	38.0	38.0	37.0	38.0
20-21	37.459261019470745	38.0	38.0	38.0	37.0	38.0
22-23	37.47397780061699	38.0	38.0	38.0	37.0	38.0
24-25	37.4897071389456	38.0	38.0	38.0	37.0	38.0
26-27	37.46048257077314	38.0	38.0	38.0	37.0	38.0
28-29	37.44121209861354	38.0	38.0	38.0	37.0	38.0
30-31	37.44336659824563	38.0	38.0	38.0	37.0	38.0
32-33	37.417667399972935	38.0	38.0	38.0	37.0	38.0
34-35	37.39261452680914	38.0	38.0	38.0	37.0	38.0
36-37	37.36608996172251	38.0	38.0	38.0	37.0	38.0
38-39	37.337283655605916	38.0	38.0	38.0	37.0	38.0
40-41	37.365293521212834	38.0	38.0	38.0	37.0	38.0
42-43	37.33866719863851	38.0	38.0	38.0	37.0	38.0
44-45	37.30386620928333	38.0	38.0	38.0	37.0	38.0
46-47	37.268086031232954	38.0	38.0	38.0	37.0	38.0
48-49	37.217505065131206	38.0	38.0	38.0	37.0	38.0
50-51	37.14682767442558	38.0	38.0	38.0	37.0	38.0
52-53	37.139822733793665	38.0	38.0	38.0	36.5	38.0
54-55	37.08186490957905	38.0	38.0	38.0	36.5	38.0
56-57	37.05733845599593	38.0	38.0	38.0	36.0	38.0
58-59	37.058865010662885	38.0	38.0	38.0	36.0	38.0
60-61	37.06728460979259	38.0	38.0	38.0	36.0	38.0
62-63	37.073840812973444	38.0	38.0	38.0	36.0	38.0
64-65	37.08311147754681	38.0	38.0	38.0	36.0	38.0
66-67	37.07907452050577	38.0	38.0	38.0	36.0	38.0
68-69	37.0692569911259	38.0	38.0	38.0	36.0	38.0
70-71	37.06815869921948	38.0	38.0	38.0	36.0	38.0
72-73	37.06961816440534	38.0	38.0	38.0	36.0	38.0
74-75	37.06396006368119	38.0	38.0	38.0	36.0	38.0
76-77	37.06068741220577	38.0	38.0	38.0	36.0	38.0
78-79	37.04045140204235	38.0	38.0	38.0	36.0	38.0
80-81	37.0372323476593	38.0	38.0	38.0	36.0	38.0
82-83	36.989488502870444	38.0	38.0	38.0	36.0	38.0
84-85	36.97203885246982	38.0	38.0	38.0	36.0	38.0
86-87	36.96983515441357	38.0	38.0	38.0	36.0	38.0
88-89	36.94934424631446	38.0	38.0	38.0	35.5	38.0
90-91	36.947400976351126	38.0	38.0	38.0	35.0	38.0
92-93	36.94744753910608	38.0	38.0	38.0	35.0	38.0
94-95	36.956570892194684	38.0	38.0	38.0	35.0	38.0
96-97	36.96795482833047	38.0	38.0	38.0	35.0	38.0
98-99	36.958084575137434	38.0	38.0	38.0	35.0	38.0
100	35.38896970583023	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	14.0
21	61.0
22	250.0
23	845.0
24	1951.0
25	4321.0
26	7808.0
27	13568.0
28	22077.0
29	33175.0
30	44074.0
31	56816.0
32	72128.0
33	96217.0
34	143038.0
35	255305.0
36	758002.0
37	4315017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.87101228550076	19.371428875553658	14.231773777437388	25.525785061508195
2	14.350534043078525	20.003289800142923	43.048150929711944	22.598025227066607
3	17.62345032019834	26.749115958652432	30.786288186920775	24.841145534228456
4	12.311390634767182	18.68402524365754	38.42071080066153	30.583873320913746
5	13.334967760410846	37.50625571813412	35.71868694386344	13.440089577591593
6	29.457543610617666	38.046887111735586	18.636434572871156	13.859134704775599
7	26.222923148792436	32.652247654846086	23.028510966542147	18.09631822981933
8	24.491323200915556	36.18798383634028	21.148353271798527	18.172339690945634
9	25.48992037490186	16.799386609132085	21.141194069158583	36.569498946807464
10-11	24.21916190631196	26.60267850321503	28.764860710587776	20.413298879885232
12-13	25.176761056547225	23.89352119630448	28.772054249931028	22.157663497217268
14-15	22.499699983054835	25.153909980495037	26.352054905762056	25.994335130688068
16-17	21.941822980216394	28.502348029614033	27.026204570180873	22.5296244199887
18-19	21.965438047917214	27.260913105650086	29.065890588498476	21.707758257934227
20-21	22.764129725646395	26.439609586924423	28.78969371287299	22.006566974556193
22-23	22.59077203650652	26.698867225832608	28.592767631020877	22.11759310664
24-25	22.53477522789632	26.933443088615284	28.715953347480294	21.815828336008103
26-27	22.449323581699133	27.082483259557232	28.736117178977793	21.73207597976584
28-29	22.031350570079475	27.031347563580372	28.837609276578764	22.099692589761393
30-31	22.918359530918174	26.725199570960484	28.637312851976038	21.719128046145308
32-33	21.981022272504163	27.22184614157192	28.686355027198857	22.11077655872506
34-35	22.259765408693	27.22812759757375	28.855933279577073	21.656173714156182
36-37	22.05249987294529	27.168832551954104	28.794144139534346	21.984523435566263
38-39	22.14155231183416	26.6171707038409	29.11106615232183	22.130210832003108
40-41	22.19688766401604	26.361160643827038	29.36720683114388	22.07474486101305
42-43	22.514188484985965	26.349879306677774	29.01606311161932	22.119869096716936
44-45	22.195310753020653	27.001027480191315	28.70583859110033	22.0978231756877
46-47	22.634039564385088	26.5091515515003	28.18405483504848	22.672754049066132
48-49	22.58143336756935	26.41080914375211	28.18941317380069	22.81834431487784
50-51	22.085976233265452	26.734088401765703	28.492315251383154	22.687620113585684
52-53	22.24036412018243	26.871125415169566	28.545942228615267	22.342568236032736
54-55	22.299477969395507	26.826708850205115	28.116921440146747	22.756891740252627
56-57	22.71337782528534	27.04808034428105	27.83670044434281	22.401841386090798
58-59	22.17937981045942	27.439560392632657	28.423248780436882	21.95781101647104
60-61	22.535598207502343	28.08719686391431	27.418313865581062	21.95889106300228
62-63	22.141615282400505	28.37707616727565	27.45416010549932	22.02714844482453
64-65	22.008936519438656	28.233304884105188	27.491734627265657	22.266023969190503
66-67	22.219097703811386	28.058717292817192	27.40020199879875	22.32198300457267
68-69	22.29319624056548	27.805158964317943	27.678229984428214	22.223414810688364
70-71	22.431432223507215	27.237760464219775	27.818167062308518	22.512640249964495
72-73	22.46391352527631	27.02006679700446	27.87202254859637	22.64399712912286
74-75	22.282315226062536	27.175240582084285	27.983541877161795	22.558902314691384
76-77	22.571266152401805	27.10696536053173	27.711511558873287	22.610256928193177
78-79	22.523479371736556	27.14888320970813	27.666796313994073	22.660841104561243
80-81	22.40688819281683	27.261024445701445	27.67400763560413	22.658079725877602
82-83	22.805453221363578	27.09491865630649	27.77584926581604	22.323778856513897
84-85	22.798252223579624	27.059151062786764	27.549570992255713	22.593025721377902
86-87	22.477392770696536	27.229793372152027	27.763094104109083	22.529719753042357
88-89	22.720734887787593	27.012382947057596	27.700376733961026	22.566505431193786
90-91	22.747957283519426	27.044878280198013	27.60667418659385	22.600490249688708
92-93	22.794699371624162	27.184478598484763	27.552441474731733	22.468380555159346
94-95	22.517076301076504	27.25168036973876	27.578769995915586	22.652473333269146
96-97	22.642735984048308	27.319017923839162	27.62910193317576	22.409144158936776
98-99	23.79824501897642	29.184503550205015	27.81112939924747	19.206122031571095
100	22.77510121299141	30.067615592128032	21.53915497638296	25.6181282184976
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1071.0
1	1070.5
2	1533.0
3	2133.5
4	2897.0
5	4322.5
6	5392.0
7	6418.5
8	7629.0
9	8167.0
10	7978.0
11	8036.0
12	9153.5
13	11794.5
14	16869.5
15	22565.0
16	26736.5
17	29005.0
18	28418.5
19	25585.0
20	22212.5
21	19258.0
22	18496.5
23	20824.5
24	25339.5
25	31921.0
26	41459.5
27	52468.5
28	62207.5
29	72765.5
30	83406.0
31	94580.5
32	108907.5
33	122714.0
34	135522.0
35	149726.0
36	169066.0
37	186642.0
38	194009.0
39	198256.5
40	205315.5
41	210147.5
42	215854.5
43	235837.5
44	261377.5
45	273230.0
46	265350.5
47	255652.5
48	254002.0
49	252850.0
50	253057.0
51	250983.0
52	239836.5
53	225548.5
54	212660.5
55	196431.5
56	178680.0
57	161487.0
58	143977.0
59	125282.0
60	102755.5
61	80298.5
62	61872.5
63	46251.0
64	34351.0
65	24983.5
66	17864.0
67	13183.5
68	9950.5
69	7441.5
70	5506.5
71	3931.5
72	2757.0
73	1967.0
74	1341.0
75	848.0
76	526.0
77	317.5
78	200.5
79	129.5
80	84.5
81	56.5
82	36.5
83	21.5
84	12.5
85	7.0
86	5.5
87	5.0
88	2.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01045552615761474
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.292138390559699E-5
22-23	1.7169355344790273E-5
24-25	4.979485806276196E-4
26-27	0.002781982234467523
28-29	0.006914469812197847
30-31	0.007170781181806645
32-33	0.002446833706719583
34-35	6.997364412458522E-4
36-37	5.2036031135585776E-5
38-39	5.583466587707143E-4
40-41	1.7565627159199827E-4
42-43	2.5646436109333056E-4
44-45	1.9615168231273535E-4
46-47	5.400762209570636E-5
48-49	8.995847189815465E-4
50-51	4.583373628826487E-4
52-53	0.0
54-55	0.007787696947921453
56-57	0.06224595119406228
58-59	0.07500833294217105
60-61	0.04532047220964916
62-63	0.009413172759167164
64-65	4.8803546885616294E-5
66-67	0.0
68-69	5.701718497955284E-4
70-71	0.0031041704834775963
72-73	0.013115111872213104
74-75	0.011878029898727833
76-77	0.01720132634099943
78-79	0.007625315367325673
80-81	0.00821346241245818
82-83	0.0412110603235714
84-85	0.0265937205481399
86-87	0.009819350548598027
88-89	0.0025431640478612272
90-91	1.1333155327240327E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	204.0
22-23	443.0
24-25	559.0
26-27	1533.0
28-29	3919.0
30-31	11304.0
32-33	14426.0
34-35	19661.0
36-37	31655.0
38-39	38596.0
40-41	38402.0
42-43	43392.0
44-45	52014.0
46-47	53265.0
48-49	49449.0
50-51	44616.0
52-53	42340.0
54-55	46143.0
56-57	48083.0
58-59	47924.0
60-61	50284.0
62-63	50697.0
64-65	56932.0
66-67	64019.0
68-69	64134.0
70-71	80145.0
72-73	51402.0
74-75	48289.0
76-77	50107.0
78-79	50885.0
80-81	50969.0
82-83	47808.0
84-85	47490.0
86-87	48200.0
88-89	50528.0
90-91	51688.0
92-93	49529.0
94-95	58186.0
96-97	175560.0
98-99	382571.0
100-101	3707281.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.27877818380385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.26653462641345	71.741960889533
2	7.575796662498158	12.315029929935784
3	2.1245085783844107	5.1803238447628495
4	0.8531711449602726	2.773788329761918
5	0.4291131352585056	1.7438895668216345
6	0.23030031621167177	1.1231116990217012
7	0.14680391238357263	0.8352429841797283
8	0.0916456884318392	0.5959079665242766
9	0.06170002237601648	0.451341218937238
>10	0.2150500629895993	2.713661828280631
>50	0.00372969810224856	0.19566087369992397
>100	0.00148685027706799	0.22439405437124882
>500	1.1947628502598162E-4	0.06438317724291567
>1k	3.982542816161453E-5	0.04130363692710799
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	1.7168351654539803E-5	0.0	1.7168351654539803E-5	0.0
12-13	0.0	4.292087913634951E-5	0.0	3.4336703309079606E-5	0.0
14-15	0.0	6.867340661815921E-5	0.0	3.4336703309079606E-5	0.0
16-17	0.0	6.867340661815921E-5	0.0	3.4336703309079606E-5	0.0
18-19	0.0	6.867340661815921E-5	0.0	5.1505054963619406E-5	0.0
20-21	0.0	6.867340661815921E-5	0.0	6.008923079088931E-5	0.0
22-23	0.0	6.867340661815921E-5	0.0	6.867340661815921E-5	0.0
24-25	0.0	7.725758244542911E-5	0.0	1.201784615817786E-4	0.0
26-27	0.0	8.5841758272699E-5	0.0	1.459309890635883E-4	0.0
28-29	0.0	8.5841758272699E-5	0.0	4.2920879136349507E-4	0.0
30-31	0.0	8.5841758272699E-5	1.7168351654539803E-5	0.0018284294512084889	0.0
32-33	0.0	8.5841758272699E-5	1.7168351654539803E-5	0.004755633408307525	0.0
34-35	0.0	1.0301010992723881E-4	1.7168351654539803E-5	0.007622748134615671	0.0
36-37	0.0	1.459309890635883E-4	1.7168351654539803E-5	0.012644490993568564	0.0
38-39	0.0	1.974360440272077E-4	1.7168351654539803E-5	0.020816626381129508	0.0
40-41	0.0	2.2318857150901742E-4	1.7168351654539803E-5	0.029709832538181127	0.0
42-43	0.0	2.403569231635572E-4	1.7168351654539803E-5	0.03817382990386925	0.0
44-45	0.0	2.403569231635572E-4	1.7168351654539803E-5	0.04752199737976617	0.0
46-47	0.0	2.5752527481809703E-4	1.7168351654539803E-5	0.057187779361272076	0.0
48-49	0.0	2.7469362647263685E-4	1.7168351654539803E-5	0.06700807650766885	0.0
50-51	0.0	2.7469362647263685E-4	1.7168351654539803E-5	0.0765193433242839	0.0
52-53	0.0	2.7469362647263685E-4	1.7168351654539803E-5	0.08594476838262624	0.0
54-55	0.0	2.7469362647263685E-4	1.7168351654539803E-5	0.0948293903638506	0.0
56-57	0.0	2.832778022999067E-4	1.7168351654539803E-5	0.10094990772869403	0.0
58-59	0.0	2.918619781271766E-4	1.7168351654539803E-5	0.1074910497090737	0.0
60-61	0.0	2.918619781271766E-4	1.7168351654539803E-5	0.11325961586499907	0.0
62-63	0.0	2.918619781271766E-4	1.7168351654539803E-5	0.11899384531761537	0.0
64-65	0.0	2.918619781271766E-4	1.7168351654539803E-5	0.12453063872620446	0.0
66-67	0.0	2.918619781271766E-4	1.7168351654539803E-5	0.1303850466404025	0.0
68-69	0.0	2.918619781271766E-4	1.7168351654539803E-5	0.1363252963128733	0.0
70-71	0.0	2.918619781271766E-4	1.7168351654539803E-5	0.14197368400721688	0.0
72-73	0.0	2.918619781271766E-4	1.7168351654539803E-5	0.1461370092834428	0.0
74-75	0.0	2.918619781271766E-4	1.7168351654539803E-5	0.14943333280111443	0.0
76-77	0.0	3.0903032978171643E-4	1.7168351654539803E-5	0.15228327917576803	0.0
78-79	0.0	3.0903032978171643E-4	1.7168351654539803E-5	0.15442073895675823	0.0
80-81	0.0	3.0903032978171643E-4	1.7168351654539803E-5	0.15510747302293984	0.0
82-83	0.0	3.0903032978171643E-4	1.7168351654539803E-5	0.15559677104509423	0.0
84-85	0.0	3.0903032978171643E-4	1.7168351654539803E-5	0.15575128620998507	0.0
86-87	0.0	3.0903032978171643E-4	1.7168351654539803E-5	0.15587146467156685	0.0
88	0.0	3.0903032978171643E-4	1.7168351654539803E-5	0.15587146467156685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7675	0.0	52.57404	1
CTTATAC	7525	0.0	46.835136	1
TATACAC	9100	0.0	40.765324	3
GTATCAA	15210	0.0	40.431713	1
TTATACA	9955	0.0	36.46272	2
ATCAACG	18750	0.0	32.3894	3
TCAACGC	19215	0.0	31.759325	4
CAACGCA	19170	0.0	31.701788	5
TATCAAC	19685	0.0	31.176031	2
AACGCAG	20745	0.0	29.437332	6
ATACACA	13030	0.0	28.95586	4
TACACAT	12720	0.0	28.566656	5
ACGCAGA	22395	0.0	27.17425	7
ACACATC	13575	0.0	26.145657	6
CGCAGAG	23735	0.0	25.675644	8
ACATCTC	14050	0.0	25.111538	8
CACATCT	14670	0.0	24.13655	7
GTGGTAT	3825	0.0	23.393557	1
GCAGAGT	27070	0.0	22.528015	9
CATCTCC	16055	0.0	22.001814	9
>>END_MODULE