FastQCFastQC Report
Fri 27 May 2016
SRR2139402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6318528
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC60700.040.2604181
GGTATCA139550.035.7114451
TATACAC73850.035.233463
GTATCAA226850.031.1234321
TTATACA86000.029.8226342
ATCAACG267200.025.8743423
CTCGATA13000.025.66901294
CAACGCA271250.025.518765
TCAACGC275850.025.07814
TATCAAC284050.024.826652
AACGCAG282950.024.699356
ACACATC106600.023.4701186
TACACAT114100.023.2430275
ACATCTC111950.022.1622648
ACGCAGA313450.022.1496667
CGCAGAG312950.022.1450828
ATACACA125100.021.6325934
CACATCT117500.021.470337
GAGTACT181950.020.31645412-13
GCAGAGT352650.019.6402479
Produced by FastQC (version 0.11.5)