##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6318528
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.899549230453676	32.0	25.0	33.0	18.0	34.0
2	32.168389694561775	33.0	32.0	33.0	32.0	34.0
3	32.700766064501096	33.0	33.0	34.0	31.0	34.0
4	32.9855820849413	33.0	33.0	34.0	33.0	34.0
5	33.25515246589079	34.0	33.0	34.0	33.0	34.0
6	36.937120639490715	38.0	38.0	38.0	35.0	38.0
7	37.07698042961905	38.0	38.0	38.0	36.0	38.0
8	37.01460767444569	38.0	38.0	38.0	35.0	38.0
9	37.24306594827149	38.0	38.0	38.0	36.0	38.0
10-11	37.342477156071794	38.0	38.0	38.0	37.0	38.0
12-13	37.43358437281594	38.0	38.0	38.0	37.0	38.0
14-15	37.412471781402246	38.0	38.0	38.0	37.0	38.0
16-17	37.43000292156654	38.0	38.0	38.0	37.0	38.0
18-19	37.44679203763915	38.0	38.0	38.0	37.0	38.0
20-21	37.46091168222391	38.0	38.0	38.0	37.0	38.0
22-23	37.47011319773097	38.0	38.0	38.0	37.0	38.0
24-25	37.486056113135675	38.0	38.0	38.0	37.0	38.0
26-27	37.45217690178386	38.0	38.0	38.0	37.0	38.0
28-29	37.428471587621004	38.0	38.0	38.0	37.0	38.0
30-31	37.438744371514346	38.0	38.0	38.0	37.0	38.0
32-33	37.417338003455015	38.0	38.0	38.0	37.0	38.0
34-35	37.38984563731901	38.0	38.0	38.0	37.0	38.0
36-37	37.36351092573922	38.0	38.0	38.0	37.0	38.0
38-39	37.34139755066123	38.0	38.0	38.0	37.0	38.0
40-41	37.357809748210514	38.0	38.0	38.0	37.0	38.0
42-43	37.32587302635083	38.0	38.0	38.0	37.0	38.0
44-45	37.29350043230354	38.0	38.0	38.0	37.0	38.0
46-47	37.26088453261987	38.0	38.0	38.0	37.0	38.0
48-49	37.20857309459991	38.0	38.0	38.0	37.0	38.0
50-51	37.14299291366542	38.0	38.0	38.0	37.0	38.0
52-53	37.140607784873765	38.0	38.0	38.0	37.0	38.0
54-55	37.083819741692025	38.0	38.0	38.0	36.5	38.0
56-57	37.06252227072537	38.0	38.0	38.0	36.0	38.0
58-59	37.068890746999934	38.0	38.0	38.0	36.0	38.0
60-61	37.07937209351458	38.0	38.0	38.0	36.0	38.0
62-63	37.086889976067795	38.0	38.0	38.0	36.0	38.0
64-65	37.09515038205255	38.0	38.0	38.0	36.0	38.0
66-67	37.09559530424531	38.0	38.0	38.0	36.0	38.0
68-69	37.09213950865001	38.0	38.0	38.0	36.0	38.0
70-71	37.086245127159465	38.0	38.0	38.0	36.0	38.0
72-73	37.09064021314133	38.0	38.0	38.0	36.0	38.0
74-75	37.0849922128389	38.0	38.0	38.0	36.0	38.0
76-77	37.08241773786058	38.0	38.0	38.0	36.0	38.0
78-79	37.06128467958018	38.0	38.0	38.0	36.0	38.0
80-81	37.060174917229105	38.0	38.0	38.0	36.0	38.0
82-83	37.009896520296365	38.0	38.0	38.0	36.0	38.0
84-85	36.99239244498506	38.0	38.0	38.0	36.0	38.0
86-87	36.989947352719525	38.0	38.0	38.0	36.0	38.0
88-89	36.97073251544072	38.0	38.0	38.0	36.0	38.0
90-91	36.97260791364549	38.0	38.0	38.0	36.0	38.0
92-93	36.9752042699352	38.0	38.0	38.0	36.0	38.0
94-95	36.984047252511374	38.0	38.0	38.0	36.0	38.0
96-97	36.99844930395152	38.0	38.0	38.0	35.5	38.0
98-99	36.98804586969351	38.0	38.0	38.0	35.0	38.0
100	35.491970142817856	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	7.0
21	62.0
22	289.0
23	890.0
24	2352.0
25	4991.0
26	9064.0
27	15262.0
28	24502.0
29	36867.0
30	49573.0
31	62169.0
32	79649.0
33	105922.0
34	153418.0
35	266977.0
36	774943.0
37	4731586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.619915271404984	19.14578838615576	14.026668869711425	25.207627472727822
2	14.661418915614268	20.06886486149391	42.422008265112936	22.84770795777888
3	18.088089504390897	26.20284344708135	30.622068937575335	25.086998110952425
4	12.43512729547135	18.378077932075318	38.24642385061837	30.94037092183496
5	13.698586126388932	37.29816501564922	35.11433359162134	13.888915266340515
6	29.925466817587893	37.46665362565459	18.625572285190476	13.982307271567048
7	26.4555763620894	32.78273040809505	22.63160027145563	18.130092958359924
8	24.886365938395777	35.567967729192624	21.031085088172436	18.514581244239164
9	25.835669320449316	16.472808223687544	21.007329555238183	36.68419290062496
10-11	24.580060419135595	26.313454652729245	28.336006424281095	20.77047850385406
12-13	25.65567486604475	23.86088975153707	28.33728045519463	22.146154927223556
14-15	22.858259075531517	25.01794721808624	26.393647381162193	25.730146325220048
16-17	22.15066547145158	28.071902189877136	27.040768039644675	22.73666429902661
18-19	21.934491704396976	27.016086658158358	29.106320332837015	21.943101304607655
20-21	23.006729541134558	26.168141985088024	28.79693625453239	22.02819221924503
22-23	22.699348099199714	26.328245407512192	28.67629118895231	22.296115304335785
24-25	22.53160489469256	26.43331056572623	28.933494579700213	22.101589959880997
26-27	22.44005375682281	26.572714398948804	28.8585034581197	22.12872838610869
28-29	21.913553576137062	26.662476162082864	28.950900365763566	22.473069896016504
30-31	22.580712951185237	26.211693465248548	29.032118647562434	22.17547493600378
32-33	22.208477367478586	26.65598362360882	28.74387493033619	22.391664078576408
34-35	22.392990683754572	26.682158789152037	29.114385338398176	21.810465188695215
36-37	22.645590564917295	26.34482816069039	28.83889743337954	22.170683841012774
38-39	22.288525888857997	26.337988280809004	29.03310868217484	22.340377148158158
40-41	22.485861622816923	26.15255277292894	29.09329622683024	22.2682893774239
42-43	22.66351835750703	26.116978635408945	28.761250410388207	22.458252596695814
44-45	22.431338727401577	26.65238121536182	28.48391617688055	22.43236388035605
46-47	22.878522089126797	26.28176646149042	27.93187727404786	22.90783417533492
48-49	22.754561735486924	26.324254916537136	28.078056362776238	22.843126985199703
50-51	22.452784500326448	26.576583187230952	28.122953301049176	22.847679011393428
52-53	22.615736830077566	26.73745813724766	27.98179815191674	22.66500688075804
54-55	22.56070314443044	26.68418236135827	27.763696976048617	22.99141751816267
56-57	22.948648554665997	26.96654254723148	27.25202837102637	22.83278052707615
58-59	22.571423932747543	27.272401435917654	27.85951016871757	22.29666446261723
60-61	22.852093398691128	27.53457860259836	27.35091780643728	22.26241019227323
62-63	22.59727913046976	27.61137739762042	27.29034784181096	22.500995630098867
64-65	22.477940302732303	27.625682096111127	27.30010324705488	22.596274354101695
66-67	22.554619727955693	27.56674920989748	27.181064666852457	22.69756639529437
68-69	22.584095058750812	27.2619576581838	27.513421628924384	22.640525654141
70-71	22.792980164977735	26.784181098569725	27.448451596567946	22.974387139884595
72-73	22.760390223969186	26.716296622589415	27.660803625621515	22.86250952781988
74-75	22.73021936254945	26.88277278246389	27.57240438559104	22.814603469395625
76-77	22.7953262753065	26.811883124290443	27.3843506962637	23.00843990413936
78-79	22.72677709063328	26.79519068368118	27.350095652419864	23.127936573265675
80-81	22.76312965815988	26.81006722552528	27.461682219540112	22.965120896774728
82-83	23.0313998564441	26.75998107364938	27.603943064833402	22.604676005073117
84-85	23.159369932967756	26.79343535433239	27.256936348074372	22.79025836462548
86-87	22.70530995642656	26.99402208950096	27.407763680032705	22.892904274039775
88-89	22.967371003072092	26.821916249141097	27.229115157218654	22.981597590568153
90-91	22.957545151249114	26.827718283780932	27.26846248877598	22.946274076193973
92-93	23.136757887690806	26.799954503519658	27.329896983592068	22.733390625197465
94-95	22.72750352279257	26.920691855930535	27.40682950434535	22.944975116931545
96-97	22.877598846231756	27.079568507573544	27.40118691088259	22.64164573531211
98-99	23.91815923172575	28.940257296087275	27.555122177406982	19.586461294779998
100	30.9808624561764	40.066749203374016	28.95238834044959	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1262.0
1	1321.5
2	1812.0
3	2454.5
4	3539.5
5	5378.5
6	6628.5
7	7555.0
8	8363.5
9	8611.0
10	8491.0
11	8642.0
12	10076.0
13	13379.0
14	19915.0
15	27486.5
16	32903.0
17	35740.5
18	35071.5
19	31304.0
20	26653.5
21	22649.0
22	20922.5
23	21986.0
24	26055.0
25	32619.5
26	42058.0
27	52637.0
28	62350.0
29	74453.0
30	86096.0
31	97114.0
32	111098.5
33	126868.5
34	142360.5
35	157974.5
36	173531.5
37	189095.5
38	199961.5
39	205581.0
40	213671.0
41	221837.0
42	232077.5
43	253150.5
44	279059.5
45	295183.0
46	301433.0
47	305066.0
48	300987.5
49	290159.0
50	280299.0
51	273782.0
52	268024.5
53	258600.0
54	246227.5
55	231859.0
56	213405.5
57	190309.0
58	168352.5
59	142333.5
60	113745.5
61	89512.5
62	69161.0
63	52595.5
64	38298.0
65	27910.0
66	20731.0
67	15925.0
68	11872.0
69	8371.5
70	5811.0
71	3918.0
72	2895.5
73	2052.5
74	1372.0
75	854.5
76	521.5
77	315.5
78	203.0
79	137.5
80	93.0
81	65.0
82	45.5
83	31.0
84	22.5
85	16.5
86	14.5
87	9.5
88	4.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010239726721160372
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.2661397204173576E-4
22-23	3.956907692465969E-5
24-25	4.352999790660282E-4
26-27	0.003245505983486074
28-29	0.00725470714465894
30-31	0.007913652064789181
32-33	0.00260723257444583
34-35	0.0011163644920497704
36-37	1.9223064601831861E-4
38-39	5.644739755724693E-4
40-41	1.4625045520454182E-4
42-43	1.6378649480440575E-4
44-45	1.5707964144505003E-4
46-47	5.0148967507982464E-5
48-49	9.207862771419707E-4
50-51	5.801968573807866E-4
52-53	0.0
54-55	0.00855029266504802
56-57	0.06536845333711264
58-59	0.07751483743381563
60-61	0.04855638176373194
62-63	0.0105893388275908
64-65	7.323779341119853E-5
66-67	1.8529605399082304E-5
68-69	6.289845466945923E-4
70-71	0.0036360029780958423
72-73	0.014317439945194037
74-75	0.012418798194236446
76-77	0.01824831393182734
78-79	0.0074253665712595346
80-81	0.008579136208456822
82-83	0.04472468363276243
84-85	0.029361121068521026
86-87	0.010539074168026609
88-89	0.002749690182109114
90-91	2.1509680593073517E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	53.0
20-21	301.0
22-23	549.0
24-25	881.0
26-27	2595.0
28-29	5146.0
30-31	14601.0
32-33	18581.0
34-35	24551.0
36-37	38813.0
38-39	47227.0
40-41	47018.0
42-43	54643.0
44-45	64848.0
46-47	64577.0
48-49	60065.0
50-51	54569.0
52-53	51503.0
54-55	56064.0
56-57	57457.0
58-59	58195.0
60-61	61872.0
62-63	57891.0
64-65	63161.0
66-67	69797.0
68-69	68963.0
70-71	80654.0
72-73	58766.0
74-75	56964.0
76-77	59672.0
78-79	61414.0
80-81	59674.0
82-83	56988.0
84-85	57991.0
86-87	58123.0
88-89	59863.0
90-91	60551.0
92-93	58783.0
94-95	66910.0
96-97	191369.0
98-99	1425128.0
100-101	2861757.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.70612735750503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.05165758525466	69.38550506172227
2	8.055201736560441	12.840978710093642
3	2.4790700103978502	5.927912099308272
4	1.0461857563285972	3.335496605341396
5	0.5128924162111828	2.04403341236135
6	0.2776796806604103	1.3279663194785996
7	0.1710391799914722	0.9543009464466456
8	0.1111515599256357	0.7087568313134457
9	0.07293168945147742	0.5231792275035719
>10	0.21776506196106007	2.5126262064400167
>50	0.00274646085666238	0.14690273341152194
>100	0.0016411393509335461	0.26269521076546987
>500	3.7723049605024E-5	0.029646635813856816
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.1652941951036696E-5	1.5826470975518348E-5	0.0	0.0	0.0
3	3.1652941951036696E-5	3.1652941951036696E-5	0.0	0.0	0.0
4	3.1652941951036696E-5	3.1652941951036696E-5	0.0	4.747941292655505E-5	0.0
5	7.913235487759174E-5	3.1652941951036696E-5	0.0	4.747941292655505E-5	0.0
6	1.7409118073070183E-4	3.1652941951036696E-5	0.0	4.747941292655505E-5	0.0
7	2.5322353560829357E-4	3.1652941951036696E-5	0.0	4.747941292655505E-5	0.0
8	2.848764775593303E-4	3.1652941951036696E-5	0.0	4.747941292655505E-5	0.0
9	2.848764775593303E-4	3.1652941951036696E-5	0.0	9.49588258531101E-5	0.0
10-11	3.007029485348486E-4	3.956617743879587E-5	0.0	9.49588258531101E-5	0.0
12-13	3.8774853890019957E-4	4.747941292655505E-5	0.0	1.0287206134086926E-4	0.0
14-15	5.855794260941788E-4	4.747941292655505E-5	0.0	1.1869853231638762E-4	0.0
16-17	5.93492661581938E-4	4.747941292655505E-5	0.0	1.4243823877966514E-4	0.0
18-19	7.121911938983257E-4	6.330588390207339E-5	0.0	2.0574412268173852E-4	0.0
20-21	8.071500197514358E-4	7.913235487759174E-5	0.0	2.4531030012053443E-4	0.0
22-23	8.783691391412683E-4	8.704559036535092E-5	0.0	3.4026912597364447E-4	0.0
24-25	0.0011711588521883577	1.1078529682862844E-4	0.0	3.956617743879587E-4	0.0
26-27	0.0011948985586516352	1.1078529682862844E-4	0.0	4.668808937777913E-4	0.0
28-29	0.001448122094259929	1.1869853231638762E-4	0.0	9.654147295066193E-4	0.0
30-31	0.0015272544491375206	1.2661176780414678E-4	0.0	0.0021603132881582547	0.0
32-33	0.0015668206265763165	1.2661176780414678E-4	0.0	0.00489037953143517	0.0
34-35	0.0018042176912090918	1.4243823877966514E-4	0.0	0.008356376675073687	0.0
36-37	0.0020495279913296262	1.4243823877966514E-4	0.0	0.012993532670900563	0.0
38-39	0.0022157059365725687	1.4243823877966514E-4	0.0	0.02020249020024917	0.0
40-41	0.0022236191720603283	1.4243823877966514E-4	0.0	0.027933721271789884	0.0
42-43	0.00239771035279103	1.503514742674243E-4	0.0	0.0352376376269916	0.0
44-45	0.0026430206529115645	1.5826470975518347E-4	0.0	0.043443662827797866	0.0
46-47	0.003038682427299523	1.5826470975518347E-4	0.0	0.05202161009652881	0.0
48-49	0.0031969471370547065	1.7409118073070183E-4	0.0	0.060710342662088385	0.0
50-51	0.0031969471370547065	1.9783088719397936E-4	0.0	0.06887680168545585	0.0
52-53	0.0032048603725424655	2.2157059365725688E-4	0.0	0.07690873570553142	0.0
54-55	0.00326025302095678	2.2157059365725688E-4	0.0	0.08536007120645822	0.0
56-57	0.00335521184680989	2.4531030012053443E-4	0.0	0.09310712874897445	0.0
58-59	0.00335521184680989	2.611367710960527E-4	0.0	0.10042687157515168	0.0
60-61	0.0033947780242486857	2.848764775593303E-4	0.0	0.10788905264010859	0.0
62-63	0.0034343442016874816	2.848764775593303E-4	0.0	0.11476565427897131	0.0
64-65	0.003505563321077314	2.848764775593303E-4	0.0	0.12172930150819938	0.0
66-67	0.003798353034124404	2.848764775593303E-4	0.0	0.12823398107913742	0.0
68-69	0.003909138330953032	3.007029485348486E-4	0.0	0.13458039594032029	0.0
70-71	0.004035750098757179	3.4818236146140366E-4	0.0	0.140175053430166	0.0
72-73	0.004178188337536844	4.1148824536347704E-4	0.0	0.14476473001306633	0.0
74-75	0.004296886869853232	4.1148824536347704E-4	0.0	0.1481990742147538	0.0
76-77	0.004352279518267546	4.273147163389954E-4	0.0	0.15175211694875768	0.0
78-79	0.0043760192247308234	4.4314118731451376E-4	0.0	0.15424478612740183	0.0
80-81	0.004929945708873965	4.4314118731451376E-4	0.0	0.1551389817375186	0.0
82-83	0.005016991299239317	4.4314118731451376E-4	0.0	0.1556058626312964	0.0
84-85	0.005135689831555703	4.4314118731451376E-4	0.0	0.15586699940239246	0.0
86-87	0.005230648657408814	4.4314118731451376E-4	0.0	0.15601735087665988	0.0
88	0.005254388363872092	4.4314118731451376E-4	0.0	0.15601735087665988	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	6070	0.0	40.260418	1
GGTATCA	13955	0.0	35.711445	1
TATACAC	7385	0.0	35.23346	3
GTATCAA	22685	0.0	31.123432	1
TTATACA	8600	0.0	29.822634	2
ATCAACG	26720	0.0	25.874342	3
CTCGATA	1300	0.0	25.669012	94
CAACGCA	27125	0.0	25.51876	5
TCAACGC	27585	0.0	25.0781	4
TATCAAC	28405	0.0	24.82665	2
AACGCAG	28295	0.0	24.69935	6
ACACATC	10660	0.0	23.470118	6
TACACAT	11410	0.0	23.243027	5
ACATCTC	11195	0.0	22.162264	8
ACGCAGA	31345	0.0	22.149666	7
CGCAGAG	31295	0.0	22.145082	8
ATACACA	12510	0.0	21.632593	4
CACATCT	11750	0.0	21.47033	7
GAGTACT	18195	0.0	20.316454	12-13
GCAGAGT	35265	0.0	19.640247	9
>>END_MODULE