FastQCFastQC Report
Fri 27 May 2016
SRR2139401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6786763
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94350.048.2053571
GTATCAA175750.038.2261
ATCAACG206950.032.0492973
TCAACGC214350.030.8041934
CAACGCA215600.030.684685
TATCAAC220900.030.393722
AACGCAG223600.029.7767436
ACGCAGA249700.026.5622847
CGCAGAG252850.026.231378
CTCGATA10550.023.58600894
GCAGAGT293150.022.5094079
GTGGTAT47550.021.3449481
CGCTACG1853.648274E-421.2374794
TGGTATC46300.020.8205682
TACATGG209250.020.7411652
ACATGGG211950.020.0140513
GTACATG225100.019.8089521
GAGTACT179800.019.60159312-13
CAGAGTA285550.019.36861210-11
AGAGTAC272150.018.92584810-11
Produced by FastQC (version 0.11.5)