##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139401_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6786763
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.0440335105263	32.0	18.0	33.0	18.0	34.0
2	32.029191972667974	33.0	32.0	33.0	30.0	34.0
3	32.653233360292674	33.0	33.0	33.0	31.0	34.0
4	32.94384421557081	33.0	33.0	34.0	32.0	34.0
5	33.2719962373815	34.0	33.0	34.0	33.0	34.0
6	36.855492670069665	38.0	37.0	38.0	35.0	38.0
7	36.96374899197158	38.0	38.0	38.0	35.0	38.0
8	36.907575673410136	38.0	38.0	38.0	35.0	38.0
9	37.189223050812295	38.0	38.0	38.0	36.0	38.0
10-11	37.3102321386499	38.0	38.0	38.0	37.0	38.0
12-13	37.4173760745734	38.0	38.0	38.0	37.0	38.0
14-15	37.39671703579453	38.0	38.0	38.0	37.0	38.0
16-17	37.415714310342054	38.0	38.0	38.0	37.0	38.0
18-19	37.43678311737127	38.0	38.0	38.0	37.0	38.0
20-21	37.44989703173482	38.0	38.0	38.0	37.0	38.0
22-23	37.46082020102398	38.0	38.0	38.0	37.0	38.0
24-25	37.48041473016726	38.0	38.0	38.0	37.0	38.0
26-27	37.45118360073804	38.0	38.0	38.0	37.0	38.0
28-29	37.43019768529865	38.0	38.0	38.0	37.0	38.0
30-31	37.43604194074082	38.0	38.0	38.0	37.0	38.0
32-33	37.415399792985994	38.0	38.0	38.0	37.0	38.0
34-35	37.39587811668496	38.0	38.0	38.0	37.0	38.0
36-37	37.371338848412705	38.0	38.0	38.0	37.0	38.0
38-39	37.367812314847114	38.0	38.0	38.0	37.0	38.0
40-41	37.350513436847706	38.0	38.0	38.0	37.0	38.0
42-43	37.31349580411253	38.0	38.0	38.0	37.0	38.0
44-45	37.27993272612804	38.0	38.0	38.0	37.0	38.0
46-47	37.24906208341078	38.0	38.0	38.0	37.0	38.0
48-49	37.19666420826006	38.0	38.0	38.0	37.0	38.0
50-51	37.12561342098508	38.0	38.0	38.0	36.5	38.0
52-53	37.11792043826918	38.0	38.0	38.0	36.0	38.0
54-55	37.06005615515255	38.0	38.0	38.0	36.0	38.0
56-57	37.03044429503689	38.0	38.0	38.0	36.0	38.0
58-59	37.036152418164136	38.0	38.0	38.0	36.0	38.0
60-61	37.04565051820194	38.0	38.0	38.0	36.0	38.0
62-63	37.052334083153404	38.0	38.0	38.0	36.0	38.0
64-65	37.055581533945244	38.0	38.0	38.0	36.0	38.0
66-67	37.05102767145628	38.0	38.0	38.0	36.0	38.0
68-69	37.041495232533634	38.0	38.0	38.0	36.0	38.0
70-71	37.03468270113422	38.0	38.0	38.0	36.0	38.0
72-73	37.037540168475836	38.0	38.0	38.0	36.0	38.0
74-75	37.02429758158654	38.0	38.0	38.0	36.0	38.0
76-77	37.014521699301746	38.0	38.0	38.0	36.0	38.0
78-79	36.986948989308466	38.0	38.0	38.0	36.0	38.0
80-81	36.98037567919735	38.0	38.0	38.0	36.0	38.0
82-83	36.92323109864929	38.0	38.0	38.0	35.5	38.0
84-85	36.89955275143036	38.0	38.0	38.0	35.0	38.0
86-87	36.893704325514335	38.0	38.0	38.0	35.0	38.0
88-89	36.866385564852735	38.0	38.0	38.0	35.0	38.0
90-91	36.86293872137266	38.0	38.0	38.0	35.0	38.0
92-93	36.862500023105355	38.0	38.0	38.0	35.0	38.0
94-95	36.86617294157131	38.0	38.0	38.0	35.0	38.0
96-97	36.87839038506087	38.0	38.0	38.0	35.0	38.0
98-99	36.86002145020399	38.0	38.0	38.0	35.0	38.0
100	35.194706409862654	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	20.0
21	66.0
22	296.0
23	988.0
24	2365.0
25	5121.0
26	9473.0
27	16626.0
28	26945.0
29	40083.0
30	53529.0
31	67675.0
32	87132.0
33	117485.0
34	175864.0
35	313954.0
36	906236.0
37	4962905.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80519682210797	19.5990194441739	14.36292972069306	25.23285401302506
2	14.031808044759847	20.52577614004664	43.61089359073468	21.831522224458826
3	17.811628312348613	26.96080590997505	30.65938798805852	24.56817778961782
4	12.0163913193963	18.51988937878043	40.14389481406673	29.319824487756534
5	13.10641022826346	37.8700567560706	35.96072236499197	13.062810650673967
6	28.779493257684113	38.86906025744526	19.32349781479035	13.027948670080272
7	25.740120879423667	33.155600099782475	23.901335585167775	17.202943435626086
8	23.810275973980527	36.95386150952965	21.599693403173205	17.636169113316612
9	25.41590151298933	17.294533491150347	21.964211804655623	35.3253531912047
10-11	23.89351889847929	27.220465780225418	29.263914181178862	19.62210114011643
12-13	24.834144053652675	24.62485429357118	29.30415427796727	21.236847374808875
14-15	21.93023389795695	25.91911637403575	27.485209075372165	24.665440652635137
16-17	21.20465971774762	28.940674663311505	28.06237671773716	21.79228890120371
18-19	21.09720053580772	28.137434591424515	29.9775533638054	20.787811508962374
20-21	22.31228342690632	27.371869990647856	29.668360720063312	20.64748586238251
22-23	21.815543540605344	27.162829180635978	29.548854869315004	21.472772409443667
24-25	21.46616474873894	27.21000092838607	29.902268946075505	21.421565376799485
26-27	21.481678047894746	27.48670855414507	29.591711308012687	21.43990208994749
28-29	21.332464460304163	27.69960336781673	29.67754514865506	21.290387023224046
30-31	21.802176081986975	27.344631609486385	29.558019039647508	21.295173268879132
32-33	21.566984581180577	27.55589429241373	29.467070548514172	21.41005057789152
34-35	21.591818601859433	27.58956493531476	29.562364524818612	21.256251938007196
36-37	21.688861623308142	27.398812994728157	29.544258496755667	21.368066885208037
38-39	21.512739331535553	27.60925285132347	29.626828055314558	21.25117976182642
40-41	21.815312416830032	27.44591780412216	29.46804611261452	21.270723666433287
42-43	21.711106848555175	27.488799847519584	29.268557799284373	21.53153550464087
44-45	21.744783802835283	27.794738935820074	29.08185545449793	21.378621806846716
46-47	21.76812783736535	27.54636005731536	28.80167154341855	21.883840561900744
48-49	21.848379165313116	27.57746312475356	28.944513837771623	21.6296438721617
50-51	21.688062848043085	27.83714782321134	28.638944606402518	21.83584472234306
52-53	21.81828315292129	27.812684812749676	28.530082801669387	21.838949232659647
54-55	21.868607740960645	27.745995185158733	28.514771672950772	21.870625400929853
56-57	21.933219191785042	27.85801752766044	28.395896303904177	21.812866976650344
58-59	21.83344948536937	27.88494200435413	28.63442658224409	21.64718192803241
60-61	21.87467789223546	27.899998979134498	28.512822893995633	21.712500234634412
62-63	21.879583267386042	27.799792500626534	28.49761584980327	21.82300838218415
64-65	21.75866027707659	27.8160623732294	28.558691373885853	21.866585975808157
66-67	21.92134431481828	27.88859184792119	28.28428154093416	21.90578229632637
68-69	21.940998196744268	27.74250304265769	28.564326016403996	21.75217274419405
70-71	21.91921244311158	27.433809352082683	28.61989721889572	22.027080985910022
72-73	21.947900580133204	27.47449922496897	28.731630343199626	21.845969851698204
74-75	21.734607325980107	27.743667997934164	28.5760961034145	21.945628572671232
76-77	21.808374297050168	27.77830943400254	28.37864615619961	22.034670112747683
78-79	21.900417268676538	27.779793590195872	28.311983048600453	22.007806092527137
80-81	21.78472507460625	27.740502924274203	28.401472496555936	22.07329950456361
82-83	22.01196940740985	27.6523323362656	28.524000061384402	21.811698194940142
84-85	22.36378805346946	27.577165240079797	28.20739286487787	21.851653841572872
86-87	21.729474692503008	27.861525251868596	28.333738116700125	22.075261938928275
88-89	22.043237136792477	27.687768375678818	28.342501185931486	21.92649330159722
90-91	22.09785387037409	27.722445683778453	28.358648298034	21.821052147813464
92-93	22.130641028765165	27.807763504474785	28.153162183791085	21.90843328296896
94-95	21.922689154902727	27.9095138052273	28.180587191374705	21.98720984849527
96-97	21.897233651659516	28.05328789726222	28.371324413328008	21.678154037750254
98-99	22.963412230063135	29.96029573028849	28.56395104366542	18.51234099598296
100	22.123843158661096	30.86374826512276	22.30285444451209	24.70955413170406
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1178.0
1	1169.0
2	1580.5
3	2247.5
4	3205.0
5	4837.0
6	6033.0
7	7004.0
8	8029.0
9	8533.0
10	8539.5
11	8648.5
12	9799.0
13	12601.0
14	18164.0
15	24815.5
16	29827.0
17	32576.0
18	32332.5
19	29568.0
20	26141.0
21	23396.0
22	23637.0
23	27358.5
24	33878.0
25	44523.5
26	61445.5
27	79824.5
28	92778.5
29	105954.5
30	119346.0
31	132809.0
32	150636.0
33	169178.5
34	188785.0
35	207979.5
36	229409.5
37	247717.0
38	252686.5
39	256976.5
40	267534.5
41	273854.5
42	275853.5
43	280134.0
44	284796.0
45	288453.5
46	289895.5
47	289840.5
48	288892.5
49	283734.0
50	274486.5
51	264326.0
52	252130.0
53	236287.0
54	217521.0
55	198871.5
56	180757.5
57	161330.5
58	141155.0
59	122063.5
60	101265.5
61	77188.5
62	55866.0
63	40311.5
64	29251.5
65	20691.5
66	14557.5
67	10438.0
68	7586.0
69	5592.0
70	4206.0
71	3039.0
72	2153.5
73	1429.5
74	923.5
75	640.5
76	443.0
77	281.5
78	163.5
79	90.5
80	55.5
81	35.0
82	24.5
83	16.5
84	12.5
85	10.5
86	6.5
87	3.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010034238708497704
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.840837952302795E-5
22-23	0.0
24-25	4.5682280329755327E-4
26-27	0.003566562445423857
28-29	0.007835460922647268
30-31	0.008454988767997468
32-33	0.002506208781090204
34-35	9.340153028770711E-4
36-37	1.3394645088786404E-4
38-39	6.887433361274851E-4
40-41	1.1306015086445037E-4
42-43	2.504898975147151E-4
44-45	2.066718258077998E-4
46-47	4.6371628724627184E-5
48-49	9.519978494212517E-4
50-51	6.141464863774042E-4
52-53	0.0
54-55	0.008150041462735496
56-57	0.06482640981891095
58-59	0.07806726487525871
60-61	0.04769447099623184
62-63	0.01031245578596855
64-65	7.539630181139615E-5
66-67	0.0
68-69	6.150628376385311E-4
70-71	0.003512919473441854
72-73	0.013757447482600708
74-75	0.012535738739711056
76-77	0.01814021855406452
78-79	0.007486457420930697
80-81	0.00855036277253827
82-83	0.04251910006841267
84-85	0.02791650181731044
86-87	0.010149041773812378
88-89	0.0023708583948871395
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	43.0
20-21	165.0
22-23	451.0
24-25	618.0
26-27	1475.0
28-29	3854.0
30-31	13137.0
32-33	17004.0
34-35	22530.0
36-37	37102.0
38-39	45692.0
40-41	45261.0
42-43	52291.0
44-45	61503.0
46-47	62938.0
48-49	58933.0
50-51	53221.0
52-53	51485.0
54-55	55218.0
56-57	57294.0
58-59	56063.0
60-61	55263.0
62-63	53102.0
64-65	55800.0
66-67	58526.0
68-69	59905.0
70-71	58988.0
72-73	55364.0
74-75	56213.0
76-77	58866.0
78-79	60427.0
80-81	60439.0
82-83	56230.0
84-85	56533.0
86-87	57649.0
88-89	60164.0
90-91	61793.0
92-93	61008.0
94-95	68801.0
96-97	201731.0
98-99	447926.0
100-101	4385757.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.37413511323113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.00734128621986	71.61521280781099
2	7.753955847833528	12.619429016472683
3	2.2150790907298727	5.407504356466375
4	0.8949431126201293	2.9130088706002413
5	0.43093618816421747	1.7533529800427914
6	0.24209478233994933	1.182015211700359
7	0.14017338894479664	0.7984541803891366
8	0.08682309287861055	0.5652123272682137
9	0.0565530042153011	0.4141756625467537
>10	0.16764376407551193	2.026992573830267
>50	0.002413870894431723	0.132454088059432
>100	0.0016869690392651464	0.24899011364658852
>500	2.2629223753248956E-4	0.12529638702787294
>1k	1.2930980693946233E-4	0.19790142413828046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4734564917030401E-5
2	0.0	0.0	0.0	0.0	1.4734564917030401E-5
3	0.0	0.0	0.0	0.0	1.4734564917030401E-5
4	0.0	4.420369475109121E-5	0.0	0.0	1.4734564917030401E-5
5	0.0	5.8938259668121604E-5	0.0	0.0	1.4734564917030401E-5
6	0.0	5.8938259668121604E-5	0.0	0.0	1.4734564917030401E-5
7	0.0	5.8938259668121604E-5	0.0	0.0	1.4734564917030401E-5
8	0.0	5.8938259668121604E-5	0.0	0.0	1.4734564917030401E-5
9	0.0	7.367282458515201E-5	0.0	0.0	1.4734564917030401E-5
10-11	0.0	7.367282458515201E-5	0.0	0.0	1.4734564917030401E-5
12-13	0.0	7.367282458515201E-5	0.0	0.0	1.4734564917030401E-5
14-15	0.0	1.399783667117888E-4	0.0	7.3672824585152004E-6	1.4734564917030401E-5
16-17	0.0	1.7681477900436483E-4	0.0	1.4734564917030401E-5	1.4734564917030401E-5
18-19	0.0	1.7681477900436483E-4	0.0	1.4734564917030401E-5	1.4734564917030401E-5
20-21	0.0	2.2101847375545603E-4	0.0	1.4734564917030401E-5	1.4734564917030401E-5
22-23	0.0	2.2101847375545603E-4	0.0	1.4734564917030401E-5	1.4734564917030401E-5
24-25	0.0	2.3575303867248641E-4	0.0	1.4734564917030401E-5	1.4734564917030401E-5
26-27	0.0	2.3575303867248641E-4	0.0	8.104010704366721E-5	1.4734564917030401E-5
28-29	0.0	2.5048760358951683E-4	0.0	3.2416042817466883E-4	1.4734564917030401E-5
30-31	0.0	2.5048760358951683E-4	0.0	0.0013555799723667969	1.4734564917030401E-5
32-33	0.0	2.7258945096506244E-4	0.0	0.003602601122213933	1.4734564917030401E-5
34-35	0.0	3.241604281746689E-4	0.0	0.006593717800371105	1.4734564917030401E-5
36-37	0.0	3.5362955800872966E-4	0.0	0.010955149015812102	1.4734564917030401E-5
38-39	0.0	3.5362955800872966E-4	0.0	0.01788039452681639	1.4734564917030401E-5
40-41	0.0	3.5362955800872966E-4	0.0	0.025564470131047748	1.4734564917030401E-5
42-43	0.0	3.609968404672449E-4	0.0	0.03305699639135771	1.4734564917030401E-5
44-45	0.0	4.0520053521833605E-4	0.0	0.041131537965890363	1.4734564917030401E-5
46-47	0.0	4.4203694751091205E-4	0.0	0.04991333865644049	1.4734564917030401E-5
48-49	0.0	4.4203694751091205E-4	0.0	0.05855516098027881	1.4734564917030401E-5
50-51	0.0	4.5677151242794244E-4	0.0	0.06695386298298614	1.4734564917030401E-5
52-53	0.0	4.5677151242794244E-4	0.0	0.07531572857340091	1.4734564917030401E-5
54-55	0.0	4.6413879488645766E-4	0.0	0.08266090918454055	1.4734564917030401E-5
56-57	0.0	4.7150607734497283E-4	0.0	0.08923989241999464	1.4734564917030401E-5
58-59	0.0	4.7150607734497283E-4	0.0	0.09595885402216049	1.4734564917030401E-5
60-61	0.0	4.7887335980348805E-4	0.0	0.1027514884489115	1.4734564917030401E-5
62-63	0.0	5.083424896375489E-4	0.0	0.10921259516502933	1.4734564917030401E-5
64-65	0.0	5.451789019301249E-4	0.0	0.11507695200200743	1.4734564917030401E-5
66-67	0.0	5.451789019301249E-4	0.0	0.12113285818290694	1.4734564917030401E-5
68-69	0.0	5.451789019301249E-4	0.0	0.1269235421952999	1.4734564917030401E-5
70-71	0.0	5.451789019301249E-4	0.0	0.13210274176363607	1.4734564917030401E-5
72-73	0.0	5.451789019301249E-4	0.0	0.13631682732990674	1.4734564917030401E-5
74-75	0.0	5.451789019301249E-4	0.0	0.1401772833381687	1.4734564917030401E-5
76-77	0.0	5.599134668471553E-4	0.0	0.1438019863077582	1.4734564917030401E-5
78-79	0.0	5.746480317641857E-4	0.0	0.14576168344172324	1.4734564917030401E-5
80-81	0.0	5.746480317641857E-4	0.0	0.14652788081740883	1.4734564917030401E-5
82-83	0.0	5.746480317641857E-4	0.0	0.14708042700179746	1.4734564917030401E-5
84-85	0.0	5.893825966812161E-4	0.0	0.14737511830013808	1.4734564917030401E-5
86-87	0.0	5.893825966812161E-4	0.0	0.14748562753701583	1.4734564917030401E-5
88	0.0	6.777899861833984E-4	0.0	0.14750772938439136	1.4734564917030401E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9435	0.0	48.205357	1
GTATCAA	17575	0.0	38.226	1
ATCAACG	20695	0.0	32.049297	3
TCAACGC	21435	0.0	30.804193	4
CAACGCA	21560	0.0	30.68468	5
TATCAAC	22090	0.0	30.39372	2
AACGCAG	22360	0.0	29.776743	6
ACGCAGA	24970	0.0	26.562284	7
CGCAGAG	25285	0.0	26.23137	8
CTCGATA	1055	0.0	23.586008	94
GCAGAGT	29315	0.0	22.509407	9
GTGGTAT	4755	0.0	21.344948	1
CGCTACG	185	3.648274E-4	21.23747	94
TGGTATC	4630	0.0	20.820568	2
TACATGG	20925	0.0	20.741165	2
ACATGGG	21195	0.0	20.014051	3
GTACATG	22510	0.0	19.808952	1
GAGTACT	17980	0.0	19.601593	12-13
CAGAGTA	28555	0.0	19.368612	10-11
AGAGTAC	27215	0.0	18.925848	10-11
>>END_MODULE