FastQCFastQC Report
Fri 27 May 2016
SRR2139400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5681360
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73000.051.2127651
GTATCAA134200.038.629211
ATCAACG160700.031.9105323
TCAACGC160850.031.8540254
CAACGCA163550.031.3807665
TATCAAC167150.031.0142962
AACGCAG176950.029.1502486
ACGCAGA194350.026.4740437
CGCAGAG207450.024.7400558
GCAGAGT239900.021.3398029
GAGTACT131200.020.37890812-13
CAGAGTA228200.019.34217810-11
GTACTTT140900.018.94551314-15
AGAGTAC213150.018.20517510-11
TACATGG166100.017.923292
AGTACTT137500.017.52100412-13
GTGGTAT38450.017.4545841
TGGTATC35300.017.305932
GTACATG176450.017.1645451
ACATGGG174250.016.6155953
Produced by FastQC (version 0.11.5)