##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139400_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5681360
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.80719845248321	25.0	18.0	32.0	18.0	33.0
2	28.174305271977133	31.0	25.0	32.0	18.0	33.0
3	30.21037586070941	31.0	29.0	33.0	27.0	33.0
4	30.399736682766097	31.0	30.0	33.0	28.0	33.0
5	31.987190919075715	33.0	32.0	33.0	31.0	33.0
6	36.64799766253151	38.0	37.0	38.0	34.0	38.0
7	36.646755354351775	38.0	37.0	38.0	34.0	38.0
8	37.18086391286593	38.0	38.0	38.0	36.0	38.0
9	37.33128141853359	38.0	38.0	38.0	37.0	38.0
10-11	37.317471344889256	38.0	38.0	38.0	37.0	38.0
12-13	37.42102595153273	38.0	38.0	38.0	37.0	38.0
14-15	37.43043118197052	38.0	38.0	38.0	37.0	38.0
16-17	37.47135703423124	38.0	38.0	38.0	37.0	38.0
18-19	37.474264084655786	38.0	38.0	38.0	37.0	38.0
20-21	37.48219075242095	38.0	38.0	38.0	37.0	38.0
22-23	37.49257804593938	38.0	38.0	38.0	37.0	38.0
24-25	37.508198291318806	38.0	38.0	38.0	37.0	38.0
26-27	37.473420621197064	38.0	38.0	38.0	37.0	38.0
28-29	37.45238227478624	38.0	38.0	38.0	37.0	38.0
30-31	37.46017825783494	38.0	38.0	38.0	37.0	38.0
32-33	37.4442085320772	38.0	38.0	38.0	37.0	38.0
34-35	37.425806995638624	38.0	38.0	38.0	37.0	38.0
36-37	37.4042725985926	38.0	38.0	38.0	37.0	38.0
38-39	37.40784111944318	38.0	38.0	38.0	37.0	38.0
40-41	37.3975812119654	38.0	38.0	38.0	37.0	38.0
42-43	37.36465361234072	38.0	38.0	38.0	37.0	38.0
44-45	37.336421461443045	38.0	38.0	38.0	37.0	38.0
46-47	37.31418682778616	38.0	38.0	38.0	37.0	38.0
48-49	37.26629736477673	38.0	38.0	38.0	37.0	38.0
50-51	37.19795300294261	38.0	38.0	38.0	37.0	38.0
52-53	37.19521242827075	38.0	38.0	38.0	37.0	38.0
54-55	37.14169048711744	38.0	38.0	38.0	36.5	38.0
56-57	37.11973713465254	38.0	38.0	38.0	36.0	38.0
58-59	37.1323333143293	38.0	38.0	38.0	36.0	38.0
60-61	37.146719863418625	38.0	38.0	38.0	36.0	38.0
62-63	37.15942885563523	38.0	38.0	38.0	36.0	38.0
64-65	37.16538412139421	38.0	38.0	38.0	36.0	38.0
66-67	37.16666498273145	38.0	38.0	38.0	36.0	38.0
68-69	37.158900570985516	38.0	38.0	38.0	36.0	38.0
70-71	37.153250226255665	38.0	38.0	38.0	36.0	38.0
72-73	37.1564661643209	38.0	38.0	38.0	36.0	38.0
74-75	37.144661262429345	38.0	38.0	38.0	36.0	38.0
76-77	37.13451564328899	38.0	38.0	38.0	36.0	38.0
78-79	37.11008490426711	38.0	38.0	38.0	36.0	38.0
80-81	37.10727517378348	38.0	38.0	38.0	36.0	38.0
82-83	37.052937189968695	38.0	38.0	38.0	36.0	38.0
84-85	37.034014072921906	38.0	38.0	38.0	36.0	38.0
86-87	37.03064799226299	38.0	38.0	38.0	36.0	38.0
88-89	37.00750572778814	38.0	38.0	38.0	36.0	38.0
90-91	37.00661858845184	38.0	38.0	38.0	36.0	38.0
92-93	37.00548385133361	38.0	38.0	38.0	36.0	38.0
94-95	37.00803678673567	38.0	38.0	38.0	36.0	38.0
96-97	37.01414205643003	38.0	38.0	38.0	36.0	38.0
98-99	37.002637128216136	38.0	38.0	38.0	35.0	38.0
100	35.48075328887618	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	10.0
21	46.0
22	249.0
23	760.0
24	1866.0
25	3862.0
26	7250.0
27	12392.0
28	20233.0
29	29999.0
30	40639.0
31	53910.0
32	71045.0
33	97331.0
34	152343.0
35	305108.0
36	1045680.0
37	3838634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47587197431601	21.569659377332187	13.966004618612446	23.988464029739358
2	14.923496421266094	20.535437035055047	40.37311777608005	24.167948767598816
3	18.238344340087583	25.545555993635325	30.692897475252405	25.523202191024687
4	13.191524564540885	17.834514975287608	38.487879662615995	30.486080797555516
5	13.92539814410634	36.9932375346748	35.07732303532957	14.004041285889294
6	30.430284298125798	37.73783037864173	18.319099652195952	13.512785671036513
7	26.88961797879381	32.61266668544152	22.87601912218201	17.621696213582663
8	24.74821169579115	36.18656448456004	20.768988411225482	18.296235408423335
9	25.876621090724754	16.909419575594576	21.13865342101187	36.075305912668796
10-11	24.635316191897715	26.666167959784275	28.317471520903446	20.381044327414564
12-13	25.402518059056284	24.05457496092485	28.52654118028078	22.01636579973809
14-15	22.71833680668009	25.268078417843615	26.229476745004714	25.784108030471575
16-17	22.18364968951096	28.367116324260387	26.988546756410436	22.460687229818213
18-19	22.182144768154107	27.470112789895378	28.80437078446006	21.54337165749046
20-21	23.162109490510616	26.708025774400774	28.504966120406326	21.624898614682284
22-23	22.87168291174061	26.519587777306526	28.354616496881608	22.25411281407126
24-25	22.616791615124367	26.7117398856355	28.44483751654092	22.226630982699213
26-27	22.438119524403856	26.973582628166433	28.496542432992637	22.091755414437078
28-29	22.462885286212742	26.98457133583205	28.40475307154202	22.14779030641318
30-31	22.617167865182623	26.83229099801099	28.26521304686468	22.28532808994171
32-33	22.540269134036244	26.912953292934766	28.241351934204967	22.30542563882402
34-35	22.579485057876596	27.14265204345892	28.15108228625791	22.12678061240657
36-37	22.609468218801066	26.840281589953552	28.197031246280353	22.353218944965032
38-39	22.50943032277423	27.016838841499137	28.297281995227685	22.17644884049895
40-41	22.62381015221194	26.77935363622407	28.422190483265886	22.174645728298103
42-43	22.67586872363533	26.843330759230255	28.02203135778153	22.458769159352887
44-45	22.68921867194475	27.140334147474633	27.836068134451285	22.33437904612933
46-47	22.666242838260402	27.126608581130412	27.625870246108004	22.581278334501185
48-49	22.683292940666906	27.01016807283964	27.68211899699881	22.624419989494637
50-51	22.60281780886823	27.228338678079318	27.528665459045836	22.640178054006622
52-53	22.68205792368189	27.282514030444656	27.35471216472399	22.680715881149464
54-55	22.807317255926034	27.117351492078477	27.331567210358777	22.74376404163671
56-57	22.78232560176805	27.113074975456986	27.514742472126656	22.589856950648308
58-59	22.67774673152415	27.058850507395483	27.675510211522404	22.587892549557964
60-61	22.782754009726084	27.307156447094428	27.365002626516166	22.545086916663323
62-63	22.706188018120788	27.34736465648074	27.34065779602442	22.605789529374054
64-65	22.480080559506842	27.20649788101701	27.461654641817873	22.85176691765827
66-67	22.706652797196575	27.35370312873887	27.164695342982636	22.774948731081917
68-69	22.716248239746854	27.274572166614462	27.30146772776985	22.707711865868834
70-71	22.72952534560543	26.977243744132263	27.38044504860589	22.912785861656417
72-73	22.780985016547646	27.087675993485465	27.35616871100866	22.77517027895823
74-75	22.524022793387093	27.263637303144066	27.303942183626127	22.90839771984271
76-77	22.830514756999012	27.14627623090598	27.210551311322085	22.81265770077292
78-79	22.872369951563503	27.155962519281022	27.139143206502524	22.832524322652947
80-81	22.725962035268537	27.220903571667726	27.13620601406272	22.91692837900102
82-83	23.11191339802037	27.01771931971722	27.26113692325117	22.609230359011242
84-85	23.034159355356092	27.033853956685	27.07194312474454	22.860043563214365
86-87	22.848005611446244	27.172706693023983	27.272972830469843	22.70631486505993
88-89	22.982107282150338	26.92450972565924	27.3090016275228	22.78438136466762
90-91	22.968517238325482	27.11741112951358	27.150368144841675	22.76370348731926
92-93	23.102371302857925	27.147693402622536	27.11766071272391	22.632274581795627
94-95	22.874124793056247	27.225843445118098	27.109229761673877	22.790802000151782
96-97	22.981081239918545	27.202138081785964	27.298109835735385	22.518670842560105
98-99	24.18241532665163	29.108860202601495	27.34257053050342	19.366153940243457
100	23.112940428999597	30.06233886585013	21.19748163595155	25.62723906919872
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	937.0
1	960.5
2	1224.0
3	1609.0
4	2186.5
5	3071.0
6	3702.0
7	4409.5
8	5420.5
9	6073.0
10	6127.5
11	6258.5
12	7119.0
13	9233.5
14	13197.5
15	17427.0
16	20367.0
17	21769.0
18	21325.5
19	19340.5
20	16865.0
21	15066.5
22	15062.5
23	17321.0
24	21691.5
25	28285.0
26	36977.0
27	47136.0
28	56149.0
29	65550.5
30	75560.5
31	85830.0
32	99306.0
33	112732.0
34	125226.0
35	138948.0
36	156884.5
37	172410.5
38	178207.5
39	183129.0
40	191948.5
41	199836.5
42	207119.5
43	216287.5
44	225962.0
45	237385.5
46	240513.5
47	240469.0
48	249984.5
49	256205.0
50	258200.0
51	256800.5
52	246467.0
53	232862.5
54	218529.5
55	202023.5
56	183246.5
57	166539.5
58	149828.0
59	127877.0
60	103006.5
61	79957.5
62	61160.5
63	45491.0
64	33539.0
65	24314.5
66	17649.0
67	13510.5
68	10203.5
69	7597.5
70	5723.5
71	4169.0
72	2986.0
73	2103.0
74	1474.5
75	998.0
76	594.0
77	353.0
78	232.5
79	158.0
80	105.0
81	67.5
82	44.5
83	31.5
84	22.0
85	13.5
86	8.0
87	7.5
88	4.5
89	1.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010208823239506034
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.0560959442043391E-4
22-23	0.0
24-25	2.9046097124339564E-4
26-27	0.0027816808359267813
28-29	0.007097102717882154
30-31	0.007544048296715175
32-33	0.0023045848877508222
34-35	8.497264146484337E-4
36-37	2.6648211891657963E-5
38-39	5.536711882551696E-4
40-41	9.881607561477972E-5
42-43	1.1749754452727619E-4
44-45	1.8205273193791128E-4
46-47	7.341133287451249E-5
48-49	7.678217203073396E-4
50-51	5.87245913607364E-4
52-53	0.0
54-55	0.00789864563737299
56-57	0.06691406091653614
58-59	0.08029093023106434
60-61	0.0466900674102943
62-63	0.009762444586203749
64-65	1.275046573528111E-4
66-67	1.9775856487400503E-5
68-69	6.781185600551271E-4
70-71	0.0033198266688315957
72-73	0.013058621338335182
74-75	0.011203071790204832
76-77	0.01703871472568082
78-79	0.006852228770717448
80-81	0.00751572573634666
82-83	0.04162435794189142
84-85	0.026057036218745073
86-87	0.009409869386475436
88-89	0.002399131776020547
90-91	1.1015044016666643E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	26.0
20-21	190.0
22-23	424.0
24-25	519.0
26-27	1208.0
28-29	3165.0
30-31	10247.0
32-33	12884.0
34-35	17326.0
36-37	28272.0
38-39	33033.0
40-41	33241.0
42-43	37257.0
44-45	43536.0
46-47	44479.0
48-49	41771.0
50-51	37407.0
52-53	35124.0
54-55	37643.0
56-57	38195.0
58-59	39708.0
60-61	38993.0
62-63	38875.0
64-65	40565.0
66-67	42679.0
68-69	43581.0
70-71	43112.0
72-73	40678.0
74-75	41477.0
76-77	43405.0
78-79	44296.0
80-81	45016.0
82-83	42002.0
84-85	42332.0
86-87	42587.0
88-89	45248.0
90-91	46210.0
92-93	45379.0
94-95	53420.0
96-97	178951.0
98-99	389646.0
100-101	3837253.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.97323429943955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.79297276247868	73.67440133164898
2	7.627252582144141	12.657156311185021
3	1.9401974311577332	4.829533681278639
4	0.7213851090508727	2.3942262269361922
5	0.33060638998795244	1.3715740728681134
6	0.1819166256987888	0.9056526484241428
7	0.10364936437553424	0.6020086096723429
8	0.07242224226278385	0.480728614060859
9	0.05238341210670584	0.3911779013520422
>10	0.17231786399101393	2.227012262690332
>50	0.003574557210767184	0.19395187256721724
>100	0.0012468939512936645	0.20213524457813986
>500	0.0	0.0
>1k	7.476558369048553E-5	0.07044122273798027
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	8.80070968922934E-6	0.0
18-19	0.0	0.0	0.0	2.640212906768802E-5	0.0
20-21	0.0	0.0	0.0	4.40035484461467E-5	0.0
22-23	0.0	0.0	0.0	7.920638720306406E-5	0.0
24-25	0.0	0.0	0.0	1.2320993564921074E-4	0.0
26-27	0.0	0.0	0.0	1.6721348409535746E-4	0.0
28-29	0.0	0.0	0.0	3.784305166368616E-4	0.0
30-31	0.0	0.0	0.0	0.0013025050340059424	0.0
32-33	0.0	0.0	0.0	0.003854710843882451	0.0
34-35	0.0	0.0	0.0	0.006688539363814298	0.0
36-37	0.0	1.760141937845868E-5	0.0	0.011836954532013461	0.0
38-39	0.0	1.760141937845868E-5	0.0	0.025794880099131196	0.0
40-41	0.0	1.760141937845868E-5	0.0	0.041143317797147166	0.0
42-43	0.0	1.760141937845868E-5	0.0	0.04969760761507808	0.0
44-45	0.0	1.760141937845868E-5	0.0	0.05998563724178718	0.0
46-47	0.0	1.760141937845868E-5	0.0	0.07044088035259163	0.0
48-49	0.0	1.760141937845868E-5	0.0	0.08141536533506062	0.0
50-51	0.0	1.760141937845868E-5	0.0	0.09178260134897279	0.0
52-53	0.0	1.760141937845868E-5	0.0	0.12102735964628188	0.0
54-55	0.0	1.760141937845868E-5	0.0	0.15915203402002337	0.0
56-57	0.0	1.760141937845868E-5	0.0	0.2134788149316361	0.0
58-59	0.0	1.760141937845868E-5	0.0	0.2478543869777659	0.0
60-61	0.0	1.760141937845868E-5	0.0	0.26078262951124376	0.0
62-63	0.0	1.760141937845868E-5	0.0	0.2718275201712266	0.0
64-65	0.0	1.760141937845868E-5	0.0	0.2792025148908008	0.0
66-67	0.0	1.760141937845868E-5	0.0	0.2856798372220736	0.0
68-69	1.760141937845868E-5	1.760141937845868E-5	0.0	0.29191073968204795	0.0
70-71	1.760141937845868E-5	1.760141937845868E-5	0.0	0.2967599307208133	0.0
72-73	1.760141937845868E-5	1.760141937845868E-5	0.0	0.3006674458228311	0.0
74-75	1.760141937845868E-5	1.760141937845868E-5	0.0	0.3033516622780461	0.0
76-77	2.640212906768802E-5	1.760141937845868E-5	0.0	0.3057894588619626	0.0
78-79	3.520283875691736E-5	1.760141937845868E-5	0.0	0.30759360434825467	0.0
80-81	3.520283875691736E-5	1.760141937845868E-5	0.0	0.3083504653815283	0.0
82-83	3.520283875691736E-5	1.760141937845868E-5	0.0	0.30879930157567903	0.0
84-85	3.520283875691736E-5	1.760141937845868E-5	0.0	0.30899291718884214	0.0
86-87	3.520283875691736E-5	1.760141937845868E-5	0.0	0.30912492783418055	0.0
88	3.520283875691736E-5	1.760141937845868E-5	0.0	0.3091337285438698	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7300	0.0	51.212765	1
GTATCAA	13420	0.0	38.62921	1
ATCAACG	16070	0.0	31.910532	3
TCAACGC	16085	0.0	31.854025	4
CAACGCA	16355	0.0	31.380766	5
TATCAAC	16715	0.0	31.014296	2
AACGCAG	17695	0.0	29.150248	6
ACGCAGA	19435	0.0	26.474043	7
CGCAGAG	20745	0.0	24.740055	8
GCAGAGT	23990	0.0	21.339802	9
GAGTACT	13120	0.0	20.378908	12-13
CAGAGTA	22820	0.0	19.342178	10-11
GTACTTT	14090	0.0	18.945513	14-15
AGAGTAC	21315	0.0	18.205175	10-11
TACATGG	16610	0.0	17.92329	2
AGTACTT	13750	0.0	17.521004	12-13
GTGGTAT	3845	0.0	17.454584	1
TGGTATC	3530	0.0	17.30593	2
GTACATG	17645	0.0	17.164545	1
ACATGGG	17425	0.0	16.615595	3
>>END_MODULE