FastQCFastQC Report
Fri 27 May 2016
SRR2139399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4120950
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49850.043.549141
GTATCAA86700.035.2536351
ATCAACG98500.030.7222483
TCAACGC98500.030.678614
CAACGCA101300.030.0427975
TATCAAC104350.029.0847722
AACGCAG105100.029.0383666
ACGCAGA121150.025.120397
CGCAGAG123000.024.8124588
GCAGAGT150100.020.3612989
GAGTACT79400.019.05630512-13
AGAGTAC130600.018.15176610-11
TACATGG114500.018.0589712
GTACATG116600.017.9180681
TATACCG2404.0455416E-717.91045
GTGGTAT27900.017.8734021
CAGAGTA145650.017.76652510-11
ACATGGG117850.017.3982413
GTACTTT88150.017.06792614-15
CATGGGG85750.016.8932154
Produced by FastQC (version 0.11.5)