##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4120950
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.6777429961538	25.0	18.0	32.0	18.0	33.0
2	27.83760783314527	30.0	25.0	32.0	18.0	33.0
3	30.018577512466784	31.0	29.0	33.0	25.0	33.0
4	30.508391511665998	31.0	30.0	33.0	28.0	33.0
5	31.93700287555054	33.0	32.0	33.0	31.0	33.0
6	36.59945036945364	38.0	37.0	38.0	34.0	38.0
7	36.672912556570694	38.0	37.0	38.0	34.0	38.0
8	37.16904912702168	38.0	38.0	38.0	36.0	38.0
9	37.31133185309213	38.0	38.0	38.0	36.0	38.0
10-11	37.33495092151082	38.0	38.0	38.0	37.0	38.0
12-13	37.41422014341353	38.0	38.0	38.0	37.0	38.0
14-15	37.42917688882417	38.0	38.0	38.0	37.0	38.0
16-17	37.463625620306004	38.0	38.0	38.0	37.0	38.0
18-19	37.473231900411314	38.0	38.0	38.0	37.0	38.0
20-21	37.48327902728995	38.0	38.0	38.0	37.0	38.0
22-23	37.4922897766633	38.0	38.0	38.0	37.0	38.0
24-25	37.50995136719908	38.0	38.0	38.0	37.0	38.0
26-27	37.47790861260259	38.0	38.0	38.0	37.0	38.0
28-29	37.45889674953693	38.0	38.0	38.0	37.0	38.0
30-31	37.46685003768046	38.0	38.0	38.0	37.0	38.0
32-33	37.447758055141335	38.0	38.0	38.0	37.0	38.0
34-35	37.431591742593966	38.0	38.0	38.0	37.0	38.0
36-37	37.410513661717125	38.0	38.0	38.0	37.0	38.0
38-39	37.40914848511956	38.0	38.0	38.0	37.0	38.0
40-41	37.398851815106035	38.0	38.0	38.0	37.0	38.0
42-43	37.370728561012385	38.0	38.0	38.0	37.0	38.0
44-45	37.346772338297384	38.0	38.0	38.0	37.0	38.0
46-47	37.32913293992097	38.0	38.0	38.0	37.0	38.0
48-49	37.288063477486816	38.0	38.0	38.0	37.0	38.0
50-51	37.2129667687088	38.0	38.0	38.0	37.0	38.0
52-53	37.202946390802595	38.0	38.0	38.0	37.0	38.0
54-55	37.135539911690785	38.0	38.0	38.0	36.5	38.0
56-57	37.11404094939877	38.0	38.0	38.0	36.0	38.0
58-59	37.125186814408906	38.0	38.0	38.0	36.0	38.0
60-61	37.135419507436126	38.0	38.0	38.0	36.0	38.0
62-63	37.138558212165314	38.0	38.0	38.0	36.0	38.0
64-65	37.14413203592524	38.0	38.0	38.0	36.0	38.0
66-67	37.13543295286297	38.0	38.0	38.0	36.0	38.0
68-69	37.12479561192882	38.0	38.0	38.0	36.0	38.0
70-71	37.11432645196069	38.0	38.0	38.0	36.0	38.0
72-73	37.114211021009595	38.0	38.0	38.0	36.0	38.0
74-75	37.096589826155245	38.0	38.0	38.0	36.0	38.0
76-77	37.083363729806464	38.0	38.0	38.0	36.0	38.0
78-79	37.059305215907855	38.0	38.0	38.0	36.0	38.0
80-81	37.053553318636006	38.0	38.0	38.0	36.0	38.0
82-83	36.99506647080456	38.0	38.0	38.0	36.0	38.0
84-85	36.97071484187512	38.0	38.0	38.0	36.0	38.0
86-87	36.96115909560527	38.0	38.0	38.0	36.0	38.0
88-89	36.93585954104025	38.0	38.0	38.0	35.0	38.0
90-91	36.931164053695554	38.0	38.0	38.0	35.0	38.0
92-93	36.929221973334535	38.0	38.0	38.0	35.0	38.0
94-95	36.937074983787355	38.0	38.0	38.0	35.0	38.0
96-97	36.93583916726793	38.0	38.0	38.0	35.0	38.0
98-99	36.917781893216436	38.0	38.0	38.0	35.0	38.0
100	35.265658408144716	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	7.0
21	55.0
22	204.0
23	667.0
24	1547.0
25	3082.0
26	5688.0
27	9131.0
28	14546.0
29	21462.0
30	29017.0
31	38071.0
32	50376.0
33	70460.0
34	111255.0
35	224376.0
36	797823.0
37	2743178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69274075152574	22.408522306749656	14.284910032880768	23.613826908843834
2	14.328108277333257	21.167713862051727	41.07740994483719	23.426767915777823
3	18.02149989686844	26.059549375750734	31.27776362246569	24.64118710491513
4	13.029325762263555	18.455428966621774	39.242626093497854	29.27261917761681
5	13.581819726034045	37.70392749244713	35.37385796964293	13.340394811875903
6	29.795969375993398	38.86516458583579	18.57571676433832	12.763149273832491
7	26.422839393829094	33.39227605285189	23.20879894199153	16.976085611327484
8	24.160205777793955	37.69409966148582	20.957837391863528	17.187857168856695
9	25.736662662735533	17.640398451813297	21.411422123539474	35.211516761911696
10-11	24.20244118467829	27.421347019497933	28.834394981739646	19.541816814084132
12-13	25.026802072337688	24.89226998628957	28.98139991992138	21.099528021451363
14-15	22.231961076936145	26.37394290151543	26.571846297577018	24.822249723971414
16-17	21.73651706523981	29.433516543515452	27.312864752059596	21.51710163918514
18-19	21.76088037952414	28.637110375034887	28.90845557456412	20.69355367087686
20-21	22.659759557569036	27.802853271535948	28.706699474383303	20.830687696511717
22-23	22.34833353638204	27.654518044073058	28.57766564527504	21.419482774269863
24-25	22.129098736904776	27.72503908528717	28.72011051036532	21.42575166744273
26-27	22.042862426174654	27.972449137752005	28.608725637286952	21.37596279878639
28-29	21.97129201010812	28.07342762461637	28.61153244462318	21.343747920652337
30-31	22.27531159183484	27.844522306872733	28.543509834692294	21.336656266600134
32-33	22.081139204215162	28.055996021501905	28.40919120712964	21.453673567153288
34-35	22.191588019241706	28.02792026803965	28.460542627268374	21.31994908545027
36-37	22.206876588245567	27.879799236821718	28.489340226582865	21.423983948349854
38-39	22.022586011542266	28.132995160667917	28.525348486685527	21.319070341104286
40-41	22.30004507843412	27.973440247179877	28.416548502401813	21.309966171984193
42-43	22.229075121406126	28.05534856395712	28.225144640525652	21.4904316741111
44-45	22.239646365289335	28.120489057218684	28.13955346393372	21.50031111355827
46-47	22.274625212850278	28.12390888554312	27.91796715500557	21.683498746601032
48-49	22.221982973900246	28.191196046813623	28.0005137185271	21.58630726075904
50-51	22.20853608491441	28.349455691508417	27.828515323802872	21.613492899774304
52-53	22.363129719873754	28.252800596833357	27.77476261927846	21.609307064014434
54-55	22.368981046558574	28.216987008538197	27.745109752850123	21.668922192053106
56-57	22.389817454704808	28.289049512770823	27.718688948747456	21.602444083776913
58-59	22.32398414663451	28.33398289160065	27.754128155487717	21.587904806277127
60-61	22.381032271837295	28.339309698208343	27.777070774116595	21.502587255837767
62-63	22.366975144300664	28.418911973743676	27.68524462893358	21.528868253022083
64-65	22.31931129186335	28.44121098740017	27.671823015068703	21.56765470566778
66-67	22.5376024270022	28.656238743957623	27.375977080737186	21.430181748302992
68-69	22.418066117815176	28.378168465772635	27.592906417550445	21.610858998861744
70-71	22.418653599317476	28.136729566821263	27.674124302256725	21.770492531604532
72-73	22.44157437094531	28.279108312946654	27.618002736652652	21.661314579455382
74-75	22.24022531304585	28.343649867324537	27.58748237622526	21.828642443404352
76-77	22.31511629017718	28.038913236354396	27.732481622728834	21.91348885073959
78-79	22.320609597127664	28.095435002035856	27.684262355437223	21.899693045399257
80-81	22.323100572024572	28.10867943659155	27.615752274232268	21.95246771715161
82-83	22.305196783569276	27.993548344469072	27.79007305464746	21.911181817314194
84-85	22.44602928266844	27.93557735873218	27.711467288837284	21.906926069762093
86-87	22.1943231539058	28.000463249889503	27.83961259381334	21.965601002391363
88-89	22.28750410299593	27.99075911620712	27.79535676871918	21.92638001207777
90-91	22.39191758388856	27.931642043921272	27.821561672115962	21.854878700074206
92-93	22.367617017565657	27.985895612405624	27.75502077148129	21.891466598547424
94-95	22.193373984431314	28.095138738140935	27.8283571293581	21.883130148069647
96-97	22.299042923824533	28.15807009578685	27.890431060643877	21.652455919744735
98-99	23.310163778338357	30.153077068296607	28.05338459728477	18.483374556080264
100	22.356452935160743	30.984997774520078	22.054740669318953	24.60380862100023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	891.0
1	826.5
2	729.5
3	759.0
4	992.5
5	1371.5
6	1666.0
7	1978.5
8	2424.0
9	2852.5
10	3133.0
11	3422.0
12	3990.5
13	4955.0
14	6843.0
15	9344.5
16	11402.5
17	12625.0
18	12906.0
19	12427.5
20	11744.5
21	11490.5
22	12377.5
23	14863.0
24	19009.5
25	25134.5
26	33266.0
27	42388.0
28	51121.0
29	60578.5
30	70634.5
31	80158.5
32	90262.5
33	100986.5
34	112136.0
35	122552.0
36	133501.5
37	143564.0
38	149383.0
39	154199.0
40	161846.5
41	168138.0
42	171543.5
43	175126.5
44	177155.5
45	178537.5
46	178416.5
47	177165.5
48	175048.5
49	169562.5
50	163466.5
51	157552.0
52	149628.0
53	140128.0
54	130467.0
55	120159.0
56	108951.5
57	96990.0
58	85217.5
59	73394.5
60	60986.0
61	49012.0
62	37901.5
63	28896.5
64	21684.5
65	15799.0
66	11467.0
67	8551.5
68	6375.5
69	4601.5
70	3345.5
71	2400.0
72	1681.5
73	1131.0
74	796.5
75	541.0
76	340.5
77	206.0
78	132.5
79	84.0
80	47.5
81	28.0
82	19.5
83	13.0
84	9.0
85	6.0
86	4.0
87	1.0
88	0.5
89	1.0
90	1.5
91	2.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01019182470061515
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.4560334324689874E-4
22-23	0.0
24-25	4.2473934353257894E-4
26-27	0.003046441113506997
28-29	0.0072240728934451675
30-31	0.007728661754267686
32-33	0.002519976597086907
34-35	8.419792809643372E-4
36-37	1.3469418542344424E-4
38-39	6.646882753542573E-4
40-41	1.733306739455645E-4
42-43	1.2454768949713E-4
44-45	2.0067756270578072E-4
46-47	3.7924311417758664E-5
48-49	0.001223124389711893
50-51	3.080655014003503E-4
52-53	0.0
54-55	0.007808479852950708
56-57	0.06880892124446598
58-59	0.08289042739113049
60-61	0.04744370721558583
62-63	0.010106031462102517
64-65	1.352506647908301E-4
66-67	0.0
68-69	6.191360932677617E-4
70-71	0.003720696220008655
72-73	0.01336208937136832
74-75	0.01151355661251104
76-77	0.016720987305164147
78-79	0.007901006291733936
80-81	0.008763608845402528
82-83	0.041197852134494915
84-85	0.026107985413311486
86-87	0.009530428173561515
88-89	0.0022516855528681413
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	69.0
20-21	281.0
22-23	324.0
24-25	546.0
26-27	1077.0
28-29	2461.0
30-31	7005.0
32-33	8965.0
34-35	12259.0
36-37	20076.0
38-39	23525.0
40-41	23605.0
42-43	26703.0
44-45	30854.0
46-47	31322.0
48-49	29620.0
50-51	27489.0
52-53	25925.0
54-55	28267.0
56-57	29095.0
58-59	29964.0
60-61	28986.0
62-63	28219.0
64-65	30418.0
66-67	31734.0
68-69	32616.0
70-71	32157.0
72-73	30518.0
74-75	31161.0
76-77	33032.0
78-79	33860.0
80-81	33907.0
82-83	32386.0
84-85	32464.0
86-87	32937.0
88-89	34329.0
90-91	34825.0
92-93	34830.0
94-95	40111.0
96-97	125494.0
98-99	277345.0
100-101	2770189.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.53543294782122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.17239665615729	82.52695440447351
2	5.939373077032053	10.635686797813959
3	1.1536867734279226	3.0988753423513193
4	0.3765375559870771	1.3485381238566967
5	0.15248016011361526	0.6826188575862819
6	0.07819855593652622	0.4200924937002774
7	0.0410396182986794	0.257214999466991
8	0.021270241800777367	0.15235522468299564
9	0.016824773048160314	0.13557720052042804
>10	0.046895829084738994	0.6139156724072692
>50	9.30412162599611E-4	0.05854241597514937
>100	3.3014214909824044E-4	0.051280940027949785
>500	3.6204801403081714E-5	0.018347527137227804
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.4266249287178927E-5	0.0	0.0	0.0
5	2.4266249287178927E-5	2.4266249287178927E-5	0.0	0.0	0.0
6	2.4266249287178927E-5	2.4266249287178927E-5	0.0	0.0	0.0
7	2.4266249287178927E-5	2.4266249287178927E-5	0.0	0.0	0.0
8	2.4266249287178927E-5	4.8532498574357854E-5	0.0	0.0	2.4266249287178927E-5
9	2.4266249287178927E-5	1.698637450102525E-4	0.0	0.0	2.4266249287178927E-5
10-11	2.4266249287178927E-5	1.698637450102525E-4	0.0	2.4266249287178927E-5	2.4266249287178927E-5
12-13	2.4266249287178927E-5	1.698637450102525E-4	0.0	2.4266249287178927E-5	2.4266249287178927E-5
14-15	2.4266249287178927E-5	2.911949914461471E-4	0.0	2.4266249287178927E-5	3.639937393076839E-5
16-17	2.4266249287178927E-5	2.911949914461471E-4	0.0	4.8532498574357854E-5	4.8532498574357854E-5
18-19	2.4266249287178927E-5	3.033281160897366E-4	0.0	1.4559749572307356E-4	6.066562321794732E-5
20-21	2.4266249287178927E-5	3.154612407333261E-4	0.0	2.1839624358461034E-4	7.279874786153678E-5
22-23	2.4266249287178927E-5	3.275943653769155E-4	0.0	2.669287421589682E-4	7.279874786153678E-5
24-25	2.4266249287178927E-5	3.39727490020505E-4	0.0	3.275943653769155E-4	9.706499714871571E-5
26-27	2.4266249287178927E-5	3.39727490020505E-4	0.0	4.1252623788204173E-4	1.2133124643589463E-4
28-29	2.4266249287178927E-5	3.39727490020505E-4	0.0	7.279874786153679E-4	1.2133124643589463E-4
30-31	4.8532498574357854E-5	3.6399373930768393E-4	0.0	0.001674371200815346	1.2133124643589463E-4
32-33	4.8532498574357854E-5	3.6399373930768393E-4	0.0	0.003943265509166576	1.4559749572307356E-4
34-35	4.8532498574357854E-5	4.6105873645639964E-4	0.0	0.007146410415074194	1.4559749572307356E-4
36-37	4.8532498574357854E-5	5.581237336051153E-4	0.0	0.01176913090428178	1.4559749572307356E-4
38-39	4.8532498574357854E-5	6.309224814666522E-4	0.0	0.023259199941761	1.4559749572307356E-4
40-41	4.8532498574357854E-5	7.279874786153679E-4	0.0	0.035938315194311996	1.698637450102525E-4
42-43	4.8532498574357854E-5	7.401206032589573E-4	0.0	0.044006843082298984	1.698637450102525E-4
44-45	4.8532498574357854E-5	7.765199771897257E-4	0.0	0.05355561217680389	1.698637450102525E-4
46-47	4.8532498574357854E-5	8.007862264769046E-4	0.0	0.06360183938169597	1.698637450102525E-4
48-49	4.8532498574357854E-5	8.007862264769046E-4	0.0	0.073235540348706	1.698637450102525E-4
50-51	4.8532498574357854E-5	8.857180989820309E-4	0.0	0.08328176755359808	1.698637450102525E-4
52-53	4.8532498574357854E-5	8.978512236256203E-4	0.0	0.10533978815564371	1.8199686965384196E-4
54-55	4.8532498574357854E-5	9.221174729127993E-4	0.0	0.13181426612795594	1.9412999429743141E-4
56-57	4.8532498574357854E-5	9.221174729127993E-4	0.0	0.16585981387786797	1.9412999429743141E-4
58-59	4.8532498574357854E-5	9.221174729127993E-4	0.0	0.19302587995486478	1.9412999429743141E-4
60-61	6.066562321794732E-5	9.342505975563887E-4	0.0	0.20376369526444144	1.9412999429743141E-4
62-63	7.279874786153678E-5	9.463837221999782E-4	0.0	0.21470777369295913	1.9412999429743141E-4
64-65	7.279874786153678E-5	0.0010919812179230517	0.0	0.22082286851332822	1.9412999429743141E-4
66-67	7.279874786153678E-5	0.0010919812179230517	0.0	0.2262706414782999	1.9412999429743141E-4
68-69	7.279874786153678E-5	0.00112838059185382	0.0	0.23168201506934083	1.9412999429743141E-4
70-71	7.279874786153678E-5	0.0011647799657845885	0.0	0.23626833618461762	1.9412999429743141E-4
72-73	7.279874786153678E-5	0.0011890462150717675	0.0	0.24027226731700216	2.669287421589682E-4
74-75	7.279874786153678E-5	0.001201179339715357	0.0	0.24328128222861234	2.669287421589682E-4
76-77	7.279874786153678E-5	0.0012497118382897147	0.0	0.24663002463024303	2.669287421589682E-4
78-79	7.279874786153678E-5	0.0012618449629333043	0.0	0.2485106589499994	2.669287421589682E-4
80-81	7.279874786153678E-5	0.0012739780875768937	0.0	0.24935997767505066	2.669287421589682E-4
82-83	7.279874786153678E-5	0.001286111212220483	0.0	0.24985743578543781	2.669287421589682E-4
84-85	7.279874786153678E-5	0.001286111212220483	0.0	0.25005156577973525	2.669287421589682E-4
86-87	7.279874786153678E-5	0.001286111212220483	0.0	0.25008796515366605	2.669287421589682E-4
88	7.279874786153678E-5	0.001310377461507662	0.0	0.25008796515366605	2.669287421589682E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4985	0.0	43.54914	1
GTATCAA	8670	0.0	35.253635	1
ATCAACG	9850	0.0	30.722248	3
TCAACGC	9850	0.0	30.67861	4
CAACGCA	10130	0.0	30.042797	5
TATCAAC	10435	0.0	29.084772	2
AACGCAG	10510	0.0	29.038366	6
ACGCAGA	12115	0.0	25.12039	7
CGCAGAG	12300	0.0	24.812458	8
GCAGAGT	15010	0.0	20.361298	9
GAGTACT	7940	0.0	19.056305	12-13
AGAGTAC	13060	0.0	18.151766	10-11
TACATGG	11450	0.0	18.058971	2
GTACATG	11660	0.0	17.918068	1
TATACCG	240	4.0455416E-7	17.9104	5
GTGGTAT	2790	0.0	17.873402	1
CAGAGTA	14565	0.0	17.766525	10-11
ACATGGG	11785	0.0	17.398241	3
GTACTTT	8815	0.0	17.067926	14-15
CATGGGG	8575	0.0	16.893215	4
>>END_MODULE