FastQCFastQC Report
Fri 27 May 2016
SRR2139398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6355933
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92200.046.0486831
GTATCAA161600.035.6804471
ATCAACG193150.029.2982883
TCAACGC196550.028.7698124
CAACGCA197050.028.7400265
TATCAAC203550.028.3060742
AACGCAG206350.027.486016
ACGCAGA231200.024.4949077
CGCAGAG236950.023.9723788
CGCTACG1803.164953E-421.68887794
GCAGAGT273100.020.7991759
GAGTACT150600.019.74937812-13
CAGAGTA266750.018.6764110-11
AGAGTAC252400.018.10182410-11
GTACTTT165150.017.97103114-15
GGACCGT5050.017.7067936
TACATGG205450.017.2452772
ACATGGG204800.017.0072983
GTGGTAT48600.016.7359371
AGTACTT167550.016.50618412-13
Produced by FastQC (version 0.11.5)