##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139398_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6355933
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.484406144621097	25.0	18.0	32.0	18.0	33.0
2	27.826338792432203	30.0	25.0	32.0	18.0	33.0
3	30.022945647790813	31.0	29.0	33.0	25.0	33.0
4	30.363979607714555	31.0	29.0	33.0	28.0	33.0
5	31.957941343938018	33.0	32.0	33.0	31.0	33.0
6	36.63631665091498	38.0	37.0	38.0	34.0	38.0
7	36.68899310297953	38.0	37.0	38.0	34.0	38.0
8	37.194369575639016	38.0	38.0	38.0	36.0	38.0
9	37.337393424380025	38.0	38.0	38.0	37.0	38.0
10-11	37.34080511861909	38.0	38.0	38.0	37.0	38.0
12-13	37.430847446000456	38.0	38.0	38.0	37.0	38.0
14-15	37.446957983981264	38.0	38.0	38.0	37.0	38.0
16-17	37.482318410216095	38.0	38.0	38.0	37.0	38.0
18-19	37.488890930725674	38.0	38.0	38.0	37.0	38.0
20-21	37.493904135797095	38.0	38.0	38.0	37.0	38.0
22-23	37.503380119446454	38.0	38.0	38.0	37.0	38.0
24-25	37.51957788410026	38.0	38.0	38.0	37.5	38.0
26-27	37.48567251353937	38.0	38.0	38.0	37.0	38.0
28-29	37.46704192356644	38.0	38.0	38.0	37.0	38.0
30-31	37.47541724572962	38.0	38.0	38.0	37.0	38.0
32-33	37.455349075255874	38.0	38.0	38.0	37.0	38.0
34-35	37.43781006592377	38.0	38.0	38.0	37.0	38.0
36-37	37.41482771161232	38.0	38.0	38.0	37.0	38.0
38-39	37.4138872753225	38.0	38.0	38.0	37.0	38.0
40-41	37.39864789550273	38.0	38.0	38.0	37.0	38.0
42-43	37.36620392793684	38.0	38.0	38.0	37.0	38.0
44-45	37.33606825274863	38.0	38.0	38.0	37.0	38.0
46-47	37.31246855475456	38.0	38.0	38.0	37.0	38.0
48-49	37.26792996001944	38.0	38.0	38.0	37.0	38.0
50-51	37.20017488231566	38.0	38.0	38.0	37.0	38.0
52-53	37.19516852723595	38.0	38.0	38.0	37.0	38.0
54-55	37.14024441764744	38.0	38.0	38.0	36.5	38.0
56-57	37.11516068116821	38.0	38.0	38.0	36.0	38.0
58-59	37.12731433132303	38.0	38.0	38.0	36.0	38.0
60-61	37.13863014314394	38.0	38.0	38.0	36.0	38.0
62-63	37.14778358687142	38.0	38.0	38.0	36.0	38.0
64-65	37.1531896879991	38.0	38.0	38.0	36.0	38.0
66-67	37.14918598267042	38.0	38.0	38.0	36.0	38.0
68-69	37.143492559198535	38.0	38.0	38.0	36.0	38.0
70-71	37.1362205592581	38.0	38.0	38.0	36.0	38.0
72-73	37.13800485407677	38.0	38.0	38.0	36.0	38.0
74-75	37.12857437026217	38.0	38.0	38.0	36.0	38.0
76-77	37.11912184108017	38.0	38.0	38.0	36.0	38.0
78-79	37.09825679490747	38.0	38.0	38.0	36.0	38.0
80-81	37.09485279786304	38.0	38.0	38.0	36.0	38.0
82-83	37.0412926149737	38.0	38.0	38.0	36.0	38.0
84-85	37.01981298143389	38.0	38.0	38.0	36.0	38.0
86-87	37.014976505561926	38.0	38.0	38.0	36.0	38.0
88-89	36.99189086404824	38.0	38.0	38.0	36.0	38.0
90-91	36.98997699492992	38.0	38.0	38.0	36.0	38.0
92-93	36.99110585515112	38.0	38.0	38.0	36.0	38.0
94-95	36.99594485154935	38.0	38.0	38.0	36.0	38.0
96-97	37.00244826231075	38.0	38.0	38.0	36.0	38.0
98-99	36.98810411208453	38.0	38.0	38.0	35.0	38.0
100	35.39492030426336	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	16.0
21	70.0
22	285.0
23	893.0
24	2128.0
25	4545.0
26	8252.0
27	14217.0
28	23756.0
29	34711.0
30	46131.0
31	58841.0
32	76627.0
33	107161.0
34	168327.0
35	341217.0
36	1224186.0
37	4244564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.46385212053053	22.742404616285288	14.233173949442198	23.56056931374198
2	14.249596007118456	21.026842120998033	41.59000898461287	23.133552887270646
3	17.647967654788054	26.495354812582196	31.508513384266323	24.348164148363427
4	12.473369370004372	18.367767564573132	40.09016457536604	29.068698490056455
5	12.980171439818514	37.952225109987786	35.874512837061054	13.193090613132643
6	29.003609698214248	39.29279934196915	18.863162969150242	12.84042799066636
7	25.677646381734988	33.45197314068603	23.86397087571565	17.00640960186333
8	23.899968737870587	37.30398039123446	21.295079731016674	17.50097113987828
9	25.484173605983575	17.129869053056414	21.724064743917218	35.661892597042794
10-11	23.882465406731	27.45114682612293	29.1415202142628	19.524867552883265
12-13	24.822335288933978	24.816128489711897	29.200244873569307	21.161291347784818
14-15	22.042153370716775	26.213610181227526	27.13674766552763	24.607488782528073
16-17	21.32445386066845	29.155522879174466	27.81285611412203	21.70716714603505
18-19	21.15945841468121	28.438326835729704	29.616619621383673	20.78559512820541
20-21	22.46501271939351	27.700854276586806	29.20999259657901	20.624140407440677
22-23	21.984223218385722	27.408159615948474	29.126469539369616	21.48114762629619
24-25	21.63020692863003	27.462849744568885	29.484890808035956	21.42205251876513
26-27	21.555229961891378	27.758256146913734	29.23108398296876	21.45542990822613
28-29	21.455999920654556	28.01304287967917	29.25753051631293	21.27342668335335
30-31	21.901820541809368	27.720879198284322	29.11853648801151	21.258763771894802
32-33	21.74380676740088	27.884133891626945	28.998263273084852	21.373796067887323
34-35	21.71296898730751	27.920707770602387	29.098196253198928	21.26812698889118
36-37	21.84396098130702	27.65661802240933	29.152128012458018	21.347292983825636
38-39	21.564719245379045	27.94755427850209	29.254293591437946	21.233432884680923
40-41	21.921290521170338	27.80723214930058	29.058842923370797	21.212634406158276
42-43	21.84124975852004	27.835136721194225	28.879690938051937	21.4439225822338
44-45	21.84083506510515	28.14045102163923	28.66286477025814	21.355849142997485
46-47	21.92621441393074	27.86678775253068	28.413264340097694	21.793733493440882
48-49	21.947311759389365	27.902625848676216	28.608886417248026	21.541175974686393
50-51	21.72836270313526	28.141306075796024	28.38820928390272	21.742121937165997
52-53	21.977317186272334	28.078608535917414	28.197770256034588	21.746304021775664
54-55	22.084950801836982	27.962950262550656	28.198557898947286	21.75354103666508
56-57	22.07980052019518	28.098157821199027	28.109954644139208	21.712087014466587
58-59	21.915013114424724	28.180441020136893	28.346251696668396	21.55829416876999
60-61	21.990704813524967	28.26267222000486	28.172838464945148	21.573784501525022
62-63	21.99594268741651	28.12842475782794	28.158539692083178	21.71709286267237
64-65	21.835135372174967	28.205817225815505	28.235321953776932	21.72372544823259
66-67	22.095104770318354	28.393136411589108	27.818163719111798	21.693595098980737
68-69	22.102289430500583	28.10488991094061	28.114713303737492	21.67810735482131
70-71	22.03307630705869	27.720388971513554	28.218290813408075	22.028243908019675
72-73	22.150106222490187	27.851095180304686	28.33229396588977	21.66650463131536
74-75	21.83665312073386	28.11852077107809	28.183789375592045	21.86103673259601
76-77	21.910479165648105	28.001465408088738	28.146141015101257	21.941914411161896
78-79	21.9782614508791	28.00524226789751	28.10716996803233	21.90932631319106
80-81	21.903990911420202	27.90115900167198	28.147253536136184	22.04759655077163
82-83	22.0713211515203	27.810680864412298	28.32115949379594	21.796838490271462
84-85	22.386001597048594	27.75892796026293	28.008679176389606	21.846391266298866
86-87	21.81226481163948	28.04260284395304	28.135564532923073	22.00956781148441
88-89	22.120016677506243	27.831059829851736	28.151564358508622	21.897359134133396
90-91	22.190532399168053	27.90325933632891	28.177921370704002	21.728286893799037
92-93	22.215161913061372	27.984867073525105	27.939110115898135	21.86086089751539
94-95	22.016692724621716	28.03272884688257	28.000230765431507	21.950347663064207
96-97	21.966892030554877	28.222871720505356	28.131012299254486	21.67922394968528
98-99	23.002686489964727	30.110614421704092	28.372322807324014	18.51437628100717
100	22.167306448496863	31.0392304858542	22.178154140422414	24.615308925226522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1107.0
1	1151.5
2	1437.0
3	1859.0
4	2470.5
5	3459.5
6	4207.0
7	4961.5
8	5901.5
9	6505.5
10	6816.5
11	7221.5
12	8190.5
13	10475.0
14	15191.5
15	20675.5
16	24529.0
17	26431.0
18	26071.0
19	24155.5
20	21964.5
21	20293.0
22	21015.5
23	25057.0
24	31729.5
25	41847.5
26	57697.0
27	76002.5
28	88580.0
29	100498.0
30	112150.5
31	122994.5
32	139378.5
33	157891.5
34	178563.5
35	197788.0
36	217629.0
37	235225.5
38	239280.5
39	240673.5
40	249839.5
41	254562.5
42	255636.0
43	263178.0
44	270066.5
45	273430.5
46	274150.5
47	275351.5
48	274163.5
49	267748.0
50	257833.5
51	244593.5
52	229906.5
53	214389.0
54	199423.5
55	183717.5
56	166724.0
57	148527.0
58	129284.5
59	112674.0
60	95060.0
61	73063.5
62	53114.5
63	38148.0
64	27083.5
65	19018.0
66	13624.0
67	9905.5
68	7124.5
69	5229.5
70	3931.0
71	2715.0
72	1792.0
73	1125.5
74	685.0
75	418.5
76	261.5
77	157.0
78	97.5
79	64.5
80	47.0
81	38.0
82	25.5
83	13.0
84	7.0
85	6.5
86	4.5
87	3.0
88	2.0
89	1.5
90	1.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009801865438166198
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.2586822525094388E-4
22-23	7.867070425699768E-6
24-25	4.877919060846375E-4
26-27	0.0033205254424639495
28-29	0.007556137972875512
30-31	0.007988721249171288
32-33	0.002644611710214343
34-35	8.94409285013175E-4
36-37	6.356125950767197E-5
38-39	6.153987308240357E-4
40-41	1.287249894385169E-4
42-43	1.457990081293477E-4
44-45	2.2045137173416605E-4
46-47	3.295749159213193E-5
48-49	0.0010974275383977008
50-51	5.36615632284138E-4
52-53	0.0
54-55	0.0085017693136048
56-57	0.06967477115974856
58-59	0.08282367087511464
60-61	0.048725144885599966
62-63	0.010116676949455864
64-65	8.005497108014169E-5
66-67	0.0
68-69	6.434099640641942E-4
70-71	0.003340467290190054
72-73	0.01390661353262265
74-75	0.011975825620786602
76-77	0.018366038538730162
78-79	0.007507694673381025
80-81	0.008443848694694474
82-83	0.044000151235333164
84-85	0.028207024835337935
86-87	0.00977143182526577
88-89	0.0024365892683584217
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	44.0
20-21	191.0
22-23	428.0
24-25	613.0
26-27	1489.0
28-29	3827.0
30-31	12220.0
32-33	15528.0
34-35	20628.0
36-37	34070.0
38-39	42089.0
40-41	40957.0
42-43	46574.0
44-45	54609.0
46-47	55249.0
48-49	51988.0
50-51	47706.0
52-53	46079.0
54-55	49724.0
56-57	50915.0
58-59	50294.0
60-61	49766.0
62-63	47594.0
64-65	49931.0
66-67	52553.0
68-69	54010.0
70-71	53227.0
72-73	50268.0
74-75	51330.0
76-77	53690.0
78-79	55186.0
80-81	55711.0
82-83	51957.0
84-85	51986.0
86-87	53263.0
88-89	55368.0
90-91	56543.0
92-93	55108.0
94-95	62258.0
96-97	187489.0
98-99	416688.0
100-101	4166785.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.73962162372219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.77261847578859	71.74500623134287
2	7.976219537625509	13.03946333986499
3	2.3064820459469804	5.655929091528444
4	0.908474650815568	2.970334968496307
5	0.42726070595626425	1.7462064219774736
6	0.22087832969574592	1.083270665652602
7	0.12734395094250445	0.7286332456277108
8	0.07372930555929307	0.48212844311971303
9	0.05255814661431949	0.38664747157487317
>10	0.13085061316858487	1.4953815640460952
>50	0.0017668023140798182	0.0956839568248765
>100	0.0015248859695137765	0.22967905776100922
>500	6.501102455860433E-5	0.036055330760035934
>1k	2.2753857849768696E-4	0.30558021142300834
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.573333136142247E-5	0.0	0.0	0.0
4	0.0	1.573333136142247E-5	0.0	0.0	0.0
5	0.0	1.573333136142247E-5	0.0	0.0	0.0
6	0.0	1.573333136142247E-5	0.0	0.0	0.0
7	0.0	1.573333136142247E-5	0.0	3.146666272284494E-5	0.0
8	0.0	1.573333136142247E-5	0.0	4.719999408426741E-5	0.0
9	0.0	1.573333136142247E-5	0.0	4.719999408426741E-5	0.0
10-11	0.0	1.573333136142247E-5	0.0	4.719999408426741E-5	0.0
12-13	0.0	2.3599997042133705E-5	0.0	6.293332544568988E-5	0.0
14-15	0.0	3.933332840355618E-5	0.0	6.293332544568988E-5	0.0
16-17	0.0	6.293332544568988E-5	0.0	6.293332544568988E-5	0.0
18-19	0.0	9.439998816853482E-5	0.0	7.866665680711236E-5	0.0
20-21	0.0	1.5733331361422468E-4	0.0	1.1013331952995729E-4	0.0
22-23	0.0	2.045333076984921E-4	0.0	1.33733316572091E-4	0.0
24-25	0.0	2.045333076984921E-4	0.0	1.809333106563584E-4	0.0
26-27	0.0	2.281333047406258E-4	0.0	2.517333017827595E-4	0.0
28-29	0.0	2.517333017827595E-4	0.0	5.270666006076528E-4	0.0
30-31	0.0	2.595999674634708E-4	0.0	0.0014395998195701558	0.0
32-33	0.0	3.461332899512943E-4	0.0	0.0032646662574951626	0.0
34-35	0.0	3.775999526741393E-4	0.0	0.005726932615557779	0.0
36-37	0.0	3.775999526741393E-4	0.0	0.009156798852347877	0.0
38-39	0.0	3.933332840355617E-4	0.0	0.014529731512273651	0.0
40-41	0.0	3.933332840355617E-4	0.0	0.02052413076097561	0.0
42-43	0.0	3.933332840355617E-4	0.0	0.026203863382449122	0.0
44-45	0.0	3.933332840355617E-4	0.0	0.032418529270211	0.0
46-47	0.0	3.933332840355617E-4	0.0	0.03901866177632772	0.0
48-49	0.0	3.933332840355617E-4	0.0	0.04561092761676373	0.0
50-51	0.0	3.933332840355617E-4	0.0	0.05239986009921753	0.0
52-53	0.0	3.933332840355617E-4	0.0	0.05886625928876217	0.0
54-55	0.0	3.933332840355617E-4	0.0	0.06503372518243977	0.0
56-57	0.0	3.933332840355617E-4	0.0	0.07190132432170068	0.0
58-59	0.0	3.933332840355617E-4	0.0	0.07861945681302808	0.0
60-61	0.0	3.933332840355617E-4	0.0	0.08476332270966355	0.0
62-63	0.0	3.933332840355617E-4	0.0	0.09112745524535895	0.0
64-65	0.0	4.090666153969842E-4	0.0	0.09730278780471727	0.0
66-67	0.0	4.090666153969842E-4	0.0	0.10311625374276287	0.0
68-69	0.0	4.090666153969842E-4	0.0	0.10943318628437398	0.0
70-71	0.0	4.090666153969842E-4	0.0	0.11514438556857035	0.0
72-73	0.0	4.090666153969842E-4	0.0	0.11988798497403921	0.0
74-75	0.0	4.090666153969842E-4	0.0	0.1233178512108293	0.0
76-77	0.0	4.090666153969842E-4	0.0	0.1266061174653666	0.0
78-79	0.0	4.090666153969842E-4	0.0	0.12868291720507438	0.0
80-81	0.0	4.3266661243911793E-4	0.0	0.12931225045953126	0.0
82-83	0.0	4.4053327811982915E-4	0.0	0.1297527837376511	0.0
84-85	0.0	4.4053327811982915E-4	0.0	0.12989438371990392	0.0
86-87	0.0	4.4053327811982915E-4	0.0	0.1300123837051146	0.0
88	0.0	4.4053327811982915E-4	0.0	0.1300202503707953	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9220	0.0	46.048683	1
GTATCAA	16160	0.0	35.680447	1
ATCAACG	19315	0.0	29.298288	3
TCAACGC	19655	0.0	28.769812	4
CAACGCA	19705	0.0	28.740026	5
TATCAAC	20355	0.0	28.306074	2
AACGCAG	20635	0.0	27.48601	6
ACGCAGA	23120	0.0	24.494907	7
CGCAGAG	23695	0.0	23.972378	8
CGCTACG	180	3.164953E-4	21.688877	94
GCAGAGT	27310	0.0	20.799175	9
GAGTACT	15060	0.0	19.749378	12-13
CAGAGTA	26675	0.0	18.67641	10-11
AGAGTAC	25240	0.0	18.101824	10-11
GTACTTT	16515	0.0	17.971031	14-15
GGACCGT	505	0.0	17.706793	6
TACATGG	20545	0.0	17.245277	2
ACATGGG	20480	0.0	17.007298	3
GTGGTAT	4860	0.0	16.735937	1
AGTACTT	16755	0.0	16.506184	12-13
>>END_MODULE