FastQCFastQC Report
Fri 27 May 2016
SRR2139397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4677975
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67450.050.04871
GTATCAA123900.038.8591081
ATCAACG155600.030.7202873
TATCAAC159550.030.3898412
TCAACGC157500.030.295644
CAACGCA157850.030.28245
AACGCAG169700.028.2430536
ACGCAGA186250.025.6419877
CGCAGAG199550.023.911628
GTGGTAT33650.023.909931
TGGTATC35300.021.7069762
GCAGAGT224200.021.3395799
GAGTACT117850.020.82211512-13
CAGAGTA214500.019.0005710-11
GTACTTT130550.018.8293314-15
AGAGTAC204450.018.53966710-11
AGTACTT127400.017.79120312-13
TACATGG160550.017.632462
GTACATG166250.017.2839781
GTACTAG11600.017.2481061
Produced by FastQC (version 0.11.5)