FastQCFastQC Report
Fri 27 May 2016
SRR2139396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4709041
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.046.5596771
GTATCAA109150.036.7651821
ATCAACG130100.030.6123473
CAACGCA131850.030.2708595
TCAACGC132650.029.9916534
AACGCAG138050.029.004226
TATCAAC138500.028.9432932
ACGCAGA155650.025.6696827
CGCAGAG157250.025.3813198
GTGGTAT30900.021.713811
GCAGAGT184450.021.6152869
TGGTATC29600.020.6461562
GAGTACT112200.019.1001212-13
CAGAGTA184850.018.66728210-11
AGAGTAC172350.018.4839310-11
GTACTTT126550.016.85004214-15
AGTACTT120600.016.40558212-13
TACATGG128750.016.3641932
TATACTG26600.016.2254895
GTACATG133550.016.1280361
Produced by FastQC (version 0.11.5)