##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139396_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4709041
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.692102064942734	25.0	18.0	32.0	18.0	33.0
2	27.97792756529408	31.0	25.0	32.0	18.0	33.0
3	30.098996802108964	31.0	29.0	33.0	27.0	33.0
4	30.4615695637392	31.0	30.0	33.0	28.0	33.0
5	31.972190303715767	33.0	32.0	33.0	31.0	33.0
6	36.64306108186359	38.0	37.0	38.0	34.0	38.0
7	36.69299502807472	38.0	37.0	38.0	34.0	38.0
8	37.195740491535325	38.0	38.0	38.0	36.0	38.0
9	37.334932101886565	38.0	38.0	38.0	37.0	38.0
10-11	37.335811049425985	38.0	38.0	38.0	37.0	38.0
12-13	37.43007918172724	38.0	38.0	38.0	37.0	38.0
14-15	37.440191431758606	38.0	38.0	38.0	37.0	38.0
16-17	37.47618570320369	38.0	38.0	38.0	37.0	38.0
18-19	37.4846613142676	38.0	38.0	38.0	37.0	38.0
20-21	37.49210707307907	38.0	38.0	38.0	37.0	38.0
22-23	37.50040797012603	38.0	38.0	38.0	37.0	38.0
24-25	37.51745808627878	38.0	38.0	38.0	37.0	38.0
26-27	37.48196221593801	38.0	38.0	38.0	37.0	38.0
28-29	37.45808388654986	38.0	38.0	38.0	37.0	38.0
30-31	37.4675101870617	38.0	38.0	38.0	37.0	38.0
32-33	37.449222774098146	38.0	38.0	38.0	37.0	38.0
34-35	37.42787442923854	38.0	38.0	38.0	37.0	38.0
36-37	37.40510448507798	38.0	38.0	38.0	37.0	38.0
38-39	37.39996700212157	38.0	38.0	38.0	37.0	38.0
40-41	37.392212723542485	38.0	38.0	38.0	37.0	38.0
42-43	37.35881815155233	38.0	38.0	38.0	37.0	38.0
44-45	37.33038820553104	38.0	38.0	38.0	37.0	38.0
46-47	37.307609682917516	38.0	38.0	38.0	37.0	38.0
48-49	37.26003652713891	38.0	38.0	38.0	37.0	38.0
50-51	37.187492184491774	38.0	38.0	38.0	37.0	38.0
52-53	37.17986608044153	38.0	38.0	38.0	37.0	38.0
54-55	37.11944427113852	38.0	38.0	38.0	36.5	38.0
56-57	37.09730697963012	38.0	38.0	38.0	36.0	38.0
58-59	37.105175544369594	38.0	38.0	38.0	36.0	38.0
60-61	37.11755534927234	38.0	38.0	38.0	36.0	38.0
62-63	37.12475847977885	38.0	38.0	38.0	36.0	38.0
64-65	37.13042322643518	38.0	38.0	38.0	36.0	38.0
66-67	37.12820791375363	38.0	38.0	38.0	36.0	38.0
68-69	37.11869544039218	38.0	38.0	38.0	36.0	38.0
70-71	37.10980583963038	38.0	38.0	38.0	36.0	38.0
72-73	37.11173852109482	38.0	38.0	38.0	36.0	38.0
74-75	37.100626966782805	38.0	38.0	38.0	36.0	38.0
76-77	37.09417359410425	38.0	38.0	38.0	36.0	38.0
78-79	37.073065359994985	38.0	38.0	38.0	36.0	38.0
80-81	37.06681327167334	38.0	38.0	38.0	36.0	38.0
82-83	37.013405575578446	38.0	38.0	38.0	36.0	38.0
84-85	36.9938622314053	38.0	38.0	38.0	36.0	38.0
86-87	36.989921298253904	38.0	38.0	38.0	36.0	38.0
88-89	36.96768242430471	38.0	38.0	38.0	36.0	38.0
90-91	36.962161514004194	38.0	38.0	38.0	36.0	38.0
92-93	36.961954249328585	38.0	38.0	38.0	35.5	38.0
94-95	36.970543314914764	38.0	38.0	38.0	35.5	38.0
96-97	36.9767465293189	38.0	38.0	38.0	35.5	38.0
98-99	36.9608894631731	38.0	38.0	38.0	35.0	38.0
100	35.32678515157043	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	15.0
21	63.0
22	235.0
23	726.0
24	1770.0
25	3496.0
26	6377.0
27	11209.0
28	17802.0
29	26315.0
30	34647.0
31	44395.0
32	57846.0
33	80431.0
34	126738.0
35	254839.0
36	898937.0
37	3143195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.12337989836996	22.801351697723593	14.451435016174205	23.623833387732237
2	14.016147204397104	21.239828354983644	41.73231490340455	23.01170953721471
3	17.430173149904622	26.52977538314064	31.736376897121943	24.303674569832793
4	12.364874291814406	18.855240376968474	40.082195079635106	28.69769025158201
5	13.009549078039456	37.80701845662418	36.07475492356087	13.10867754177549
6	28.79157348598154	39.49407108581131	19.079596036645253	12.634759391561893
7	25.56887909873794	33.54869919374242	23.93716682441287	16.945254883106774
8	23.569512348692655	37.69232419084905	21.574711284102218	17.163452176356078
9	25.253591973397555	17.322189379960804	21.933128210181223	35.49109043646042
10-11	23.570882054329108	27.532941420556757	29.455933809028206	19.44024271608593
12-13	24.5328613617932	25.06517781433629	29.495941955060488	20.90601886881002
14-15	21.705279270237828	26.515589904611154	27.240047389691448	24.539083435459577
16-17	21.14119414122748	29.531840984183404	27.974007871241724	21.35295700334739
18-19	21.01369472043246	28.692561818850166	29.751100064747792	20.54264339596958
20-21	21.980262379705824	27.888042349750275	29.548509156022636	20.583186114521265
22-23	21.719890162165836	27.751053355872884	29.398323550149087	21.130732931812197
24-25	21.433039955916396	27.884599761720008	29.580913728051783	21.101446554311813
26-27	21.431616660230258	28.081499806482885	29.40309848300427	21.083785050282586
28-29	21.27934292129168	28.204161312899394	29.48923546131438	21.02726030449455
30-31	21.61674947057573	27.947209968926334	29.346976578778637	21.089063981719296
32-33	21.467192960344406	28.129110781523526	29.30257493468296	21.101121323449114
34-35	21.578434659349117	28.18871947966589	29.327524406815247	20.90532145416974
36-37	21.532653656019452	28.071048942833553	29.286218767656198	21.1100786334908
38-39	21.4043216630079	28.21925262000793	29.37624268884505	21.000183028139123
40-41	21.67311569769939	28.03463862936324	29.262803727450265	21.029441945487104
42-43	21.582779997864463	28.095339579369693	29.11212011570314	21.2097603070627
44-45	21.61409006210975	28.30813248525474	28.909274086642984	21.16850336599253
46-47	21.735571582506676	28.21244871665403	28.5762289415073	21.475750759331987
48-49	21.739287148294302	28.21621181129589	28.706187799427752	21.33831324098206
50-51	21.604034745396415	28.39511885187162	28.544650001184962	21.456196401546997
52-53	21.762027138076075	28.393018505755922	28.471692614492206	21.373261741675798
54-55	21.783461106253174	28.364900760588014	28.41194484316195	21.439693289996864
56-57	21.879940013349632	28.407019247161756	28.30773498383308	21.405305755655533
58-59	21.750129950853573	28.583359086470274	28.44637299269792	21.220137969978232
60-61	21.793108051919603	28.65648638935565	28.302602349840793	21.247803208883955
62-63	21.803622393273827	28.630747567422794	28.21090655546404	21.354723483839344
64-65	21.702496385576705	28.60287087625803	28.297808175494062	21.396824562671206
66-67	21.850827930294777	28.739340548097086	28.106100412157442	21.303731109450695
68-69	21.81548321176018	28.52710475179817	28.294267821722123	21.363144214719522
70-71	21.806029544465577	28.215776915186574	28.354038102871186	21.62415543747666
72-73	21.898761607063737	28.335197877026495	28.399064807434875	21.366975708474893
74-75	21.770817301352565	28.404723104498608	28.34600300726043	21.4784565868884
76-77	21.75020258730217	28.248988327684486	28.353398220265806	21.647410864747535
78-79	21.828570217556468	28.298375702473027	28.30618893755706	21.566865142413445
80-81	21.76077602412745	28.302159596582605	28.276969870740466	21.66009450854948
82-83	21.90067655065397	28.163680810948378	28.508727509953395	21.42691512844426
84-85	22.0619508309317	28.126971158914117	28.27872201850884	21.532355991645346
86-87	21.69777907841981	28.30210795709848	28.337578804354685	21.66253416012702
88-89	21.929292175186198	28.162121836293725	28.269031113526854	21.63955487499322
90-91	22.002938990494098	28.1865885650871	28.30691748767219	21.503554956746612
92-93	22.031544160163534	28.200795404621648	28.261335311894438	21.50632512332038
94-95	21.7285247594279	28.389575532839096	28.307710242112538	21.574189465620464
96-97	21.88196892747136	28.415106545356917	28.37169774224896	21.33122678492277
98-99	22.902160878030543	30.333942823443937	28.54316664310782	18.220729655417696
100	21.865616307537152	31.246656051516926	22.61241677595441	24.275310864991518
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1106.0
1	1133.0
2	1221.0
3	1381.5
4	1850.5
5	2557.0
6	3043.0
7	3559.5
8	4166.0
9	4608.5
10	4842.0
11	5156.0
12	6012.5
13	7836.5
14	11397.5
15	15341.0
16	18288.0
17	20056.5
18	20086.0
19	18849.5
20	17567.5
21	17098.0
22	18246.0
23	21649.5
24	27132.5
25	35040.0
26	45836.5
27	57236.5
28	67126.0
29	78824.5
30	90270.0
31	100211.0
32	112568.0
33	125466.0
34	138474.5
35	151085.5
36	162687.0
37	172739.5
38	178673.5
39	182824.0
40	188235.0
41	192137.0
42	194104.5
43	196759.5
44	200344.0
45	204226.0
46	204130.5
47	201533.5
48	197602.5
49	190389.0
50	182646.5
51	174380.0
52	164142.5
53	152884.5
54	141356.5
55	127903.5
56	114336.5
57	100380.5
58	85954.0
59	73076.5
60	59735.0
61	46227.5
62	34857.0
63	25816.5
64	18694.0
65	13386.5
66	9702.0
67	7358.0
68	5430.5
69	3798.5
70	2793.5
71	2042.0
72	1395.5
73	908.5
74	603.0
75	406.0
76	252.5
77	148.0
78	100.5
79	66.0
80	32.5
81	15.5
82	13.0
83	10.5
84	8.0
85	5.5
86	3.0
87	2.5
88	2.5
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010320572702594859
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.0618006184776242E-4
22-23	0.0
24-25	4.566357457010399E-4
26-27	0.003419889097882112
28-29	0.008010750980281122
30-31	0.008507073472084412
32-33	0.0028551920121955053
34-35	0.0011641356506396873
36-37	1.0718985606653318E-4
38-39	5.820648989427545E-4
40-41	1.6270796382806713E-4
42-43	1.310141785727621E-4
44-45	1.540145204889917E-4
46-47	3.3291855380624124E-5
48-49	0.001029821042848279
50-51	5.078390601591793E-4
52-53	0.0
54-55	0.008569400458165058
56-57	0.06812336080328739
58-59	0.0810813581133308
60-61	0.048407617275117484
62-63	0.010269306948518672
64-65	8.360492991995904E-5
66-67	0.0
68-69	5.475050680719134E-4
70-71	0.003551334478444137
72-73	0.014171336672242544
74-75	0.012045623613328971
76-77	0.01774615266191026
78-79	0.007710506727097976
80-81	0.008834325572109634
82-83	0.043359682244405555
84-85	0.027681504295040626
86-87	0.009833587788491222
88-89	0.002416880784401344
90-91	1.3575011986735585E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	167.0
22-23	399.0
24-25	492.0
26-27	1205.0
28-29	2823.0
30-31	8336.0
32-33	10771.0
34-35	14799.0
36-37	23956.0
38-39	29465.0
40-41	29217.0
42-43	32837.0
44-45	38903.0
46-47	39269.0
48-49	37098.0
50-51	33991.0
52-53	32378.0
54-55	34933.0
56-57	36464.0
58-59	35631.0
60-61	35899.0
62-63	34812.0
64-65	36682.0
66-67	39057.0
68-69	39956.0
70-71	41092.0
72-73	36182.0
74-75	36812.0
76-77	38728.0
78-79	39803.0
80-81	39701.0
82-83	37508.0
84-85	37598.0
86-87	38343.0
88-89	40259.0
90-91	40524.0
92-93	39561.0
94-95	45458.0
96-97	139017.0
98-99	309440.0
100-101	3119441.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.95937097228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65097040806772	77.0634102333199
2	7.418461243833457	12.753725242043238
3	1.7627412547238601	4.5457238832885185
4	0.591250324915038	2.0329402406741135
5	0.2512396021493553	1.0798199082042232
6	0.12479129096769351	0.643618852464102
7	0.06903872671009673	0.4154167864508942
8	0.036770780359745264	0.2528634519906856
9	0.026229309186343513	0.20291894268259802
>10	0.06681155588620723	0.8298951181166825
>50	0.001002524234627539	0.05581295128116868
>100	6.569172984368631E-4	0.10014062688305744
>500	3.606166742972788E-5	0.023713762600802882
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.2741447780981307E-4	0.0	0.0	2.1235746301635514E-5	0.0
7	1.2741447780981307E-4	0.0	0.0	2.1235746301635514E-5	0.0
8	1.2741447780981307E-4	2.1235746301635514E-5	0.0	2.1235746301635514E-5	0.0
9	1.2741447780981307E-4	6.370723890490654E-5	0.0	2.1235746301635514E-5	0.0
10-11	1.3803235096063085E-4	6.370723890490654E-5	0.0	2.1235746301635514E-5	0.0
12-13	1.486502241114486E-4	6.370723890490654E-5	0.0	2.1235746301635514E-5	0.0
14-15	1.486502241114486E-4	8.494298520654205E-5	0.0	3.185361945245327E-5	0.0
16-17	2.5482895561962615E-4	8.494298520654205E-5	0.0	4.247149260327103E-5	0.0
18-19	2.5482895561962615E-4	8.494298520654205E-5	0.0	4.247149260327103E-5	0.0
20-21	2.5482895561962615E-4	8.494298520654205E-5	0.0	4.247149260327103E-5	0.0
22-23	2.5482895561962615E-4	1.0617873150817757E-4	0.0	7.43251120557243E-5	0.0
24-25	2.5482895561962615E-4	1.0617873150817757E-4	0.0	1.0617873150817757E-4	0.0
26-27	2.760647019212617E-4	1.0617873150817757E-4	0.0	1.0617873150817757E-4	0.0
28-29	2.760647019212617E-4	1.1679660465899533E-4	0.0	2.760647019212617E-4	0.0
30-31	2.760647019212617E-4	1.8050384356390188E-4	0.0	8.812834715178739E-4	0.0
32-33	2.760647019212617E-4	1.9112171671471963E-4	0.0	0.0024421108246880843	0.0
34-35	2.760647019212617E-4	2.229753361671729E-4	0.0	0.004809896537320444	0.0
36-37	2.760647019212617E-4	2.5482895561962615E-4	0.0	0.008324412550241122	0.0
38-39	2.760647019212617E-4	2.5482895561962615E-4	0.0	0.013357284423728737	0.0
40-41	2.760647019212617E-4	2.5482895561962615E-4	0.0	0.019441325739147315	0.0
42-43	2.973004482228972E-4	2.5482895561962615E-4	0.0	0.024644083583048013	0.0
44-45	3.07918321373715E-4	2.654468287704439E-4	0.0	0.030791832137371494	0.0
46-47	3.185361945245327E-4	2.8668257507207944E-4	0.0	0.036982052184298245	0.0
48-49	3.185361945245327E-4	2.973004482228972E-4	0.0	0.04302362200711355	0.0
50-51	4.0347917973107474E-4	2.973004482228972E-4	0.0	0.04919260630773867	0.0
52-53	4.247149260327103E-4	2.973004482228972E-4	0.0	0.055117379525894974	0.0
54-55	4.247149260327103E-4	2.973004482228972E-4	0.0	0.06116956722186109	0.0
56-57	4.459506723343458E-4	2.973004482228972E-4	0.0	0.067816355814273	0.0
58-59	4.459506723343458E-4	2.973004482228972E-4	0.0	0.07426140481681939	0.0
60-61	4.7780429178679906E-4	2.973004482228972E-4	0.0	0.08068521807306414	0.0
62-63	5.733651501441589E-4	2.973004482228972E-4	0.0	0.08672678789587943	0.0
64-65	5.733651501441589E-4	3.185361945245327E-4	0.0	0.09266217898718657	0.0
66-67	5.733651501441589E-4	3.185361945245327E-4	0.0	0.09870374881000188	0.0
68-69	5.733651501441589E-4	3.2915406767535047E-4	0.0	0.10417195348267301	0.0
70-71	6.052187695966122E-4	3.397719408261682E-4	0.0	0.1092366789756131	0.0
72-73	6.583081353507009E-4	3.397719408261682E-4	0.0	0.11327147077292382	0.0
74-75	6.583081353507009E-4	3.8224343342943925E-4	0.0	0.11620200376254952	0.0
76-77	6.583081353507009E-4	4.3533279918352803E-4	0.0	0.11932365846888995	0.0
78-79	6.583081353507009E-4	4.671864186359813E-4	0.0	0.1212030220165847	0.0
80-81	6.583081353507009E-4	4.7780429178679906E-4	0.0	0.12171267992782395	0.0
82-83	6.689260085015187E-4	4.990400380884346E-4	0.0	0.12221171996591237	0.0
84-85	6.795438816523364E-4	5.2027578439007E-4	0.0	0.122466548921532	0.0
86-87	6.795438816523364E-4	5.521294038425234E-4	0.0	0.12259396339934182	0.0
88	6.795438816523364E-4	5.733651501441589E-4	0.0	0.12259396339934182	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6315	0.0	46.559677	1
GTATCAA	10915	0.0	36.765182	1
ATCAACG	13010	0.0	30.612347	3
CAACGCA	13185	0.0	30.270859	5
TCAACGC	13265	0.0	29.991653	4
AACGCAG	13805	0.0	29.00422	6
TATCAAC	13850	0.0	28.943293	2
ACGCAGA	15565	0.0	25.669682	7
CGCAGAG	15725	0.0	25.381319	8
GTGGTAT	3090	0.0	21.71381	1
GCAGAGT	18445	0.0	21.615286	9
TGGTATC	2960	0.0	20.646156	2
GAGTACT	11220	0.0	19.10012	12-13
CAGAGTA	18485	0.0	18.667282	10-11
AGAGTAC	17235	0.0	18.48393	10-11
GTACTTT	12655	0.0	16.850042	14-15
AGTACTT	12060	0.0	16.405582	12-13
TACATGG	12875	0.0	16.364193	2
TATACTG	2660	0.0	16.225489	5
GTACATG	13355	0.0	16.128036	1
>>END_MODULE