FastQCFastQC Report
Fri 27 May 2016
SRR2139395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5831459
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76150.050.526851
CTTATAC64050.043.5739141
GTATCAA138700.036.9874531
TATACAC91400.034.028583
ATCAACG169800.030.0849083
TCAACGC171450.029.7953784
CAACGCA175000.029.288345
TATCAAC178250.029.0197412
TTATACA102950.028.7648982
AACGCAG182600.028.1393286
ACACATC112600.025.0109546
ACATCTC113450.024.7860098
ACGCAGA207000.024.7606747
CGCAGAG209400.024.5175768
TACACAT124400.024.1454835
GTGGTAT36250.021.6278931
CACATCT128900.021.5837757
ATACACA151350.021.5069144
CATCTCC130700.021.4495149
GCAGAGT243600.021.0579629
Produced by FastQC (version 0.11.5)